LOCUS       sequence-45615-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, 
            Waterman ML
  TITLE     A uniform human Wnt expression library reveals a shared secretory 
            pathway and unique signaling activities.
  JOURNAL   Differentiation. 2012 Sep;84(2):203-13. doi: 
            10.1016/j.diff.2012.06.004. Epub 2012 Jul 9.
  PUBMED    22784633
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.diff.2012.06.004"; journalName: "Differentiation"; date: 
            "2012-09"; volume: "84"; issue: "2"; pages: "203-13"
COMMENT     Sequence Label: pcDNA-Wnt7B-V5
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..57)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    83..107
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttactaaccg gtacgcgtag aatcgagacc gaggagaggg ttagggatag gcttaccttc
       61 gaaccgcggg ccctctagat caaccacttt gtacaagaaa gctgggtcgg cgcgcccacc
      121 cttcagaatg cacttgcagg tgaagacctc ggtgcgctcg ctgcaggtgt tgcacttgac
      181 gaagcagcac cagtggaatt tgcagttgca ctgccacacc ttggtgtact ggtgggtgtt
      241 gtagcctcgg ccgcagcaca tggtgtcaca gccgtccgcg ccgggcgacg tgcggttgca
      301 gagacggccc tgcgtgccca cgctgcccgt ggccgcgtcc tcctcgcagt agttgggcga
      361 cttctcaatg tacaccaggt ctgtctccat gggcttctga tagctgcgca gctgtttgat
      421 gcgcaggaag gtgggctgcc gcagacggct ggcccgcacc acctccacct gcacggccgc
      481 gttgtacttc tccttcagca ggtggcccac ctctcggaac ttgggcagcg tggtccagca
      541 ggttttggtg gtgcaggagc cagacacgcc gtggcacttg cactccagct gcatccggtc
      601 ctctagaacc ttcctgccgg cctcattgtt atgcaggttc atgaggcgcc gcgcgttctt
      661 cttgatctcc cgagcgtcca cgaagcgccg ggagaagtcg atgccgtaac gcacgtcggc
      721 cgagcagccg ccccacttcc agccctcggc ttggttgtag tagccctgct tctcgcggtc
      781 gcagccgcag ttgctcaggt tcccttggct gcaggcagcg gtgacggcgt gcgccacgcc
      841 agccgcggtg atggcgtacg tgaaggcagc ctcacggctc cctactcgga
//
