LOCUS       sequence-456150-        8865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral or overpexression of cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8865)
  AUTHORS   Klingbeil O, Skopelitis D, Tonelli C, Yoshimoto T, Alpsoy A, 
            Panepinto MC, Minicozzi F, Merrill JR, Cafiero AM, Aggarwal D, Russo
            S, Ha T, Demerdash OE, Wee TL, Spector DL, Lyons SK, Tuveson DA, 
            Cifani P, Vakoc CR
  TITLE     MARK2/MARK3 kinases are catalytic co-dependencies of YAP/TAZ in 
            human cancer.
  JOURNAL   Cancer Discov. 2024 Jul 26. doi: 10.1158/2159-8290.CD-23-1529.
  PUBMED    39058094
REFERENCE   2  (bases 1 to 8865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2024 Jul 26. doi: 10.1158/2159-8290.CD-23-1529."
COMMENT     Sequence Label: HA-MST2-S444A
FEATURES             Location/Qualifiers
     source          1..8865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1723..1840
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1909..2120
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2149..2175
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2149..2175
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2179..2205
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     RBS             2865..2873
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    join(3697..4271,4272..4274,4275..4283)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3864..3881)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4091..4110
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    4722..5310
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4775..4795)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5193..5204)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5382..5615
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5693..5814
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5730..5749)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5784..5803
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5854..5989
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6009..6028)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6010..6028)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6038..6055)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6038..6054)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6047..6069)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6196..6651
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6283..6302)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6493..6514
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6677..6781
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6782..6850,6851..7642)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7000..7019)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7813..8401
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8302..8321
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8555..8572
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8689..8710
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8725..8730,8731..8748,8749..8755)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8763..8779
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8768..8790
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8787..8803
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8787..8803
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8822..8842
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8824..8842
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagaccac aattttaaaa gaaaaggggg
     1741 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     1801 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     1861 cagagatcca ctttggcgcc ggctcgagtg gctccggtgc ccgtcagtgg gcagagcgca
     1921 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     1981 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2041 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2101 ggtttgccgc cagaacacag gtgtcgtgac gcgggatccg ccaccatgta cccctacgac
     2161 gtgcccgact acgccggcta tccgtatgat gtcccggact atgcaggaag cggagagcag
     2221 ccgccggcgc ctaagagtaa actaaaaaag ctgagtgaag acagtttgac taagcagcct
     2281 gaagaagttt ttgatgtatt agagaagctt ggagaagggt cttatggaag tgtatttaaa
     2341 gcaatacaca aggaatccgg tcaagttgtc gcaattaaac aagtacctgt tgaatcagat
     2401 cttcaggaaa taatcaaaga aatttccata atgcagcaat gtgacagccc atatgttgta
     2461 aagtactatg gcagttattt taagaataca gacctctgga ttgttatgga gtactgtggc
     2521 gctggctctg tctcagacat aattagatta cgaaacaaga cattaataga agatgaaatt
     2581 gcaaccattc ttaaatctac attgaaagga ctagaatatt tgcactttat gagaaaaata
     2641 cacagagata taaaagctgg aaatattctc ctcaatacag aaggacatgc aaaattggca
     2701 gattttggag tggctggtca gttaacagat acaatggcaa aacgcaatac tgtaatagga
     2761 actccatttt ggatggctcc tgaggtgatt caagaaatag gctataactg tgtggccgac
     2821 atctggtccc ttggcattac ttctatagaa atggctgaag gaaaacctcc ttatgctgat
     2881 atacatccaa tgagggctat ttttatgatt cccacaaatc caccaccaac attcagaaag
     2941 ccagaacttt ggtccgatga tttcaccgat tttgttaaaa agtgtttggt gaagaatcct
     3001 gagcagagag ctactgcaac acaactttta cagcatcctt ttatcaagaa tgccaaacct
     3061 gtatcaatat taagagacct gatcacagaa gctatggaga tcaaagctaa aagacatgag
     3121 gaacagcaac gagaattgga agaggaagaa gaaaattcgg atgaagatga gctggattcc
     3181 cacaccatgg tgaagactag tgtggagagt gtgggcacca tgcgggccac aagcacgatg
     3241 agtgaagggg cccagaccat gattgaacat aatagcacga tgttggaatc cgacttgggg
     3301 accatggtga taaacagtga ggatgaggaa gaagaagatg gaactatgaa aagaaatgca
     3361 acctcaccac aagtacaaag accatctttc atggactact ttgataagca agacttcaag
     3421 aataagagtc acgaaaactg taatcagaac atgcatgaac ccttccctat gtccaaaaac
     3481 gtttttcctg ataactggaa agttcctcaa gatggagact ttgacttttt gaaaaatcta
     3541 gctttagaag aactacagat gcggttaaaa gcactggacc ccatgatgga acgggagata
     3601 gaagaacttc gtcagagata cactgcgaaa agacagccca ttctggatgc gatggatgca
     3661 aagaaaagaa ggcagcaaaa cttttgagca attccgcccc tctccctccc ccccccctaa
     3721 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
     3781 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
     3841 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
     3901 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg
     3961 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
     4021 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
     4081 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
     4141 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
     4201 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     4261 acgatgataa tatggccaca accatgaaga ccttcaacat ctctcagcag gaccttgagc
     4321 tggtggaagt tgccactgag aaaatcacca tgctctatga ggacaacaag caccatgttg
     4381 gtgctgccat caggaccaag acaggagaaa tcatctctgc tgtccacatt gaagcctaca
     4441 ttggcagggt cactgtgtgt gcagaggcca ttgccattgg gtctgcagtc tccaatgggc
     4501 agaaggactt tgacaccatt gtggctgtga ggcaccccta cagtgatgag gtggacaggt
     4561 ccatcagagt ggtgtccccc tgtggcatgt gcagggagct catctcagac tatgcccctg
     4621 attgctttgt tctgattgag atgaatggca agctggtcaa gacaaccatt gaggagctga
     4681 ttccactgaa atacaccaga aactaaacgc gttaagtcga caatcaacct ctggattaca
     4741 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     4801 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     4861 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     4921 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     4981 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     5041 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     5101 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     5161 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     5221 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     5281 gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga cttacaaggc
     5341 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     5401 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     5461 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     5521 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     5581 cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt catgtcatct
     5641 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     5701 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     5761 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     5821 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     5881 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     5941 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac
     6001 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
     6061 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     6121 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     6181 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     6241 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     6301 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     6361 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     6421 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     6481 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     6541 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     6601 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     6661 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     6721 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     6781 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     6841 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     6901 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     6961 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     7021 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     7081 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     7141 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     7201 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     7261 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     7321 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     7381 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     7441 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     7501 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     7561 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     7621 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     7681 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     7741 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     7801 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     7861 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     7921 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     7981 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     8041 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     8101 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     8161 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     8221 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     8281 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     8341 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     8401 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     8461 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     8521 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     8581 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     8641 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     8701 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     8761 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
     8821 cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagc
//
