LOCUS       sequence-45616-e         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, 
            Waterman ML
  TITLE     A uniform human Wnt expression library reveals a shared secretory 
            pathway and unique signaling activities.
  JOURNAL   Differentiation. 2012 Sep;84(2):203-13. doi: 
            10.1016/j.diff.2012.06.004. Epub 2012 Jul 9.
  PUBMED    22784633
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.diff.2012.06.004"; journalName: "Differentiation"; date: 
            "2012-09"; volume: "84"; issue: "2"; pages: "203-13"
COMMENT     Sequence Label: pcDNA-Wnt8B-V5
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..62)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    88..112
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 actcattact aaccggtacg cgtagaatcg agaccgagga gagggttagg gataggctta
       61 ccttcgaacc gcgggccctc tagatcaacc actttgtaca agaaagctgg gtcggcgcgc
      121 ccacccttca gaatgcaggg ttttctcccg ggtttgtgcg cagcgccccc ccgcggccgc
      181 tctgcgcggc tacagaagta cttggtgacc ctccggcggc actgctcgca gcggactgcg
      241 cagcaccagt ggaacttgca gttgcagctg gacacggtct cggcccggcg ctcctccacc
      301 gccagcccgc agtccccgca gagccggcgg cagctgcggc gttcccagcg acccagggcc
      361 cgcccgcgcc ttaggcactc tcggccttcg gtgcccagca gccctagcgt tttgttctcc
      421 aggcagtagt ccggggagtc ctccaggtgc accagctccc gggtagagat ggagcgaaag
      481 gtgtcggcga tggcgccgcg gccggccgcg ctgttgccag caccctgcag caggtccacc
      541 ttgagtgctg cgtggtactt ctccttcagg tgcgcgccca cctcgcggaa ctcgggcagc
      601 tgcagccaac aggtctgcgt ggtgcagctg ccagacacgc cgtggcactt gcacgtgcgt
      661 ttcatggtgc ccttcaccgc cttgcggcca gcctcgttgt tgtgcaggtt catggctgcc
      721 cgtgcatcct gtcctgtttc cagggcatcg acaaactgct tggaaatcgc ctctccgaag
      781 cccacattgt cactgcagcc tccccacagc cagccttgtc cccccagttg cccgttgcgg
      841 gagtcatcac agccacagtt atcaaaatct ccaaggctgc agtttctagt cagggtgtac
      901 atgactccag ca
//
