LOCUS       sequence-456172-        2348 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pTDH3 Promoter for yeast expression with Nterminal tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Lai Z, Flanigan SF, Boudes M, Davidovich C
  TITLE     Modular cloning of multigene vectors for the baculovirus system and 
            yeast.
  JOURNAL   J Mol Biol. 2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943.
  PUBMED    39814169
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943."
COMMENT     Sequence Label: pMY.001
FEATURES             Location/Qualifiers
     source          1..2348
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(74..93)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             764..1423
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(830..849)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1494..1528
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     promoter        complement(1548..2214)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(1655..1678)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     rep_origin      complement(join(2342..2348,1..582))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
       61 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      121 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      181 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      241 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      301 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      361 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      421 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      481 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      541 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      601 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgatt
      661 gatcgggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt
      721 attttttgag ttatcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
      781 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
      841 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
      901 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
      961 gatgaatgct catccggaat ttcgtatggc aatgaaagac ggtgagctgg tgatatggga
     1021 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     1081 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     1141 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtttc
     1201 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     1261 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     1321 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     1381 tgaattacaa cagtactgcg atgagtggca gggcggggcg taatttgata tcgagctcgc
     1441 ttggactcct gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa
     1501 cgctcggttg ccgccgggcg ttttttattg gtctggtctc atcttgattt gtttgtttat
     1561 gtgtgtttat tcgaaactaa gttcttggtg ttttaaaact aaaaaaaaga ctaactataa
     1621 aagtagaatt taagaagttt aagaaataga tttacagaat tacaatcaat acctaccgtc
     1681 tttatatact tattagtcaa gtaggggaat aatttcaggg aactggtttc aacctttttt
     1741 ttcagctttt tccaaatcag agagagcaga aggtaataga aggtgtaaga aaatgagata
     1801 gatacatgcg tgggtcaatt gccttgtgtc atcatttact ccaggcaggt tgcatcactc
     1861 cattgaggtt gtgcccgttt tttgcctgtt tgtgcccctg ttctctgtag ttgcgctaag
     1921 agaatggacc tatgaactga tggttggtga agaaaacaat attttggtgc tgggattctt
     1981 tttttttctg gatgccagct taaaaagcgg gctccattat atttagtgga tgccaggaat
     2041 aaactgttca cccagacacc tacgatgtta tatattctgt gtaacccgcc ccctattttg
     2101 ggcatgtacg ggttacagca gaattaaaag gctaattttt tgactaaata aagttaggaa
     2161 aatcactact attaattatt tacgtattct ttgaaatggc agtattgata atgataaact
     2221 cgaactgcgt ttgagaccga ctacggttat ccacagaatc aggggataac gcaggaaaga
     2281 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     2341 ttttccat
//
