LOCUS       sequence-456173-        2367 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pCCW12 Promoter for yeast expression with Nterminal tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Lai Z, Flanigan SF, Boudes M, Davidovich C
  TITLE     Modular cloning of multigene vectors for the baculovirus system and 
            yeast.
  JOURNAL   J Mol Biol. 2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943.
  PUBMED    39814169
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943."
COMMENT     Sequence Label: pMY.002
FEATURES             Location/Qualifiers
     source          1..2367
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(549..1137)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(629..648)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1319..1978
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1385..1404)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2049..2083
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 caaagatagc ggcgtaggtg gccgcgaaat gggaaattcc aaaacaaacg gtttttttac
       61 tcctgagaaa tacttgtacg ggataatcca gggcctacca cccacgcttc gaggattggc
      121 ttttattttt tttttttggt ggcgttttat ttctttcccg ctttctggga cttgtgcgga
      181 gttttgagag gggcgcgcgg caaaggattc ccaaaacgga aatcagacgc caatagccag
      241 cactcaaagc agttctggac ccattccgat tttcccattt ggttcttgcg cgtgctgatt
      301 ccgacacgcg cgtctataaa tagcatgaag tatccgcaca ccgcagcgtt agtgaggtga
      361 gggtggcagc aagctaattc ccgcatctgg aatctgaact gccccttttg gactaaccgt
      421 gtggttcatg ggtgcgtttg agaccgacta cggttatcca cagaatcagg ggataacgca
      481 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      541 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      601 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
      661 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
      721 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
      781 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
      841 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
      901 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
      961 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     1021 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1081 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1141 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     1201 attttggtca tgattgatcg ggcacgtaag aggttccaac tttcaccata atgaaataag
     1261 atcactaccg ggcgtatttt ttgagttatc gagattttca ggagctaagg aagctaaaat
     1321 ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca
     1381 ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat
     1441 tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca
     1501 cattcttgcc cgcctgatga atgctcatcc ggaatttcgt atggcaatga aagacggtga
     1561 gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac
     1621 gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc
     1681 gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa
     1741 tatgtttttc gtttcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc
     1801 caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga
     1861 caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt
     1921 cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaatt
     1981 tgatatcgag ctcgcttgga ctcctgttga tagatccagt aatgacctca gaactccatc
     2041 tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtctg gtctcatctt
     2101 gattattgat atagtgttta agcgaatgac agaagattaa tttcttggta tgttaggaaa
     2161 gaataaagga gaataagaat aattagaaca atgtaggatg gaaagaaaga ttatcaagca
     2221 tgccgacttt atatacttga acggaggcaa aggatgcaaa attttctcac atttctttct
     2281 gccgttatgt tggaagtaag actcccatta tcgcaatact gcaacacgaa tatgcaaaat
     2341 ttgctgagtt atcgcagata gttgttg
//
