LOCUS       sequence-456176-        2344 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pHHF2 Promoter for yeast expression with Nterminal tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Lai Z, Flanigan SF, Boudes M, Davidovich C
  TITLE     Modular cloning of multigene vectors for the baculovirus system and 
            yeast.
  JOURNAL   J Mol Biol. 2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943.
  PUBMED    39814169
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943."
COMMENT     Sequence Label: pMY.004
FEATURES             Location/Qualifiers
     source          1..2344
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(709..743)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(814..1473)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1388..1407
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1655..2243
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2144..2163
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acgtgtggag tgtttgcttg gattctttag taaaagggga agaacagttg
       61 gaagggccaa agtggaagtc acaaaacagt ggtcctatat aaaagaacaa gaaaaagatt
      121 atttatatac aactgcggtc acaagaagca acgcgagaga gcacaacacg ctgttatcac
      181 gcaaactatg ttttgacacc gagccatagc cgtgattgtg cgtcacattg ggcgataatg
      241 aacgctaaat gaccaactcc catccgtagg agccccttag ggcgtgccaa tagtttcacg
      301 cgcttaatgc gaagtgctcg gaacggacaa ctgtggtcgt ttggcaccgg gaaagtggta
      361 ctagaccgag agtttcgcat ttgtatggca ggacgttctg ggagcttcgc gtctaaagct
      421 ttttcgggcg cgaaatgcag accagaccag aacaaaacaa ctgacaagaa ggcgtttaat
      481 ttaatatgtt gttcactcgc gcctgggctg ttgttattcg gctagataca tacgtgtttg
      541 tgcgtatgta gttatatcat atataagtat attaggatga ggcggtgaaa gagatttttt
      601 ttttttcgct taatttattc ttttctctat cttttttcct acatcttgtt caaaagagta
      661 gcaaaaacaa caatcaatac aataaaatat caagatgaga ccagaccaat aaaaaacgcc
      721 cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct
      781 atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcatcgca
      841 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg
      901 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt
      961 gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact
     1021 cacccaggga ttggctgaaa cgaaaaacat attctcaata aaccctttag ggaaataggc
     1081 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc
     1141 gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta
     1201 acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc
     1261 cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt
     1321 atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt
     1381 acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc
     1441 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct
     1501 cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc
     1561 tcttacgtgc ccgatcaatc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     1621 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     1681 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     1741 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     1801 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     1861 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     1921 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     1981 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     2041 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     2101 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     2161 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     2221 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     2281 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     2341 gtag
//
