LOCUS       sequence-456177-        5581 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses hRad1 in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5581)
  AUTHORS   Lindsey-Boltz LA, Bermudez VP, Hurwitz J, Sancar A
  TITLE     Purification and characterization of human DNA damage checkpoint Rad
            complexes.
  JOURNAL   Proc Natl Acad Sci U S A. 2001 Sep 25;98(20):11236-41. doi: 
            10.1073/pnas.201373498.
  PUBMED    11572977
REFERENCE   2  (bases 1 to 5581)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1073/pnas"; 
            journalName: "Proc Natl Acad Sci U S A"; date: "2001-09-25- 25"; 
            volume: "98"; issue: "20"; pages: "11236-41"
COMMENT     Sequence Label: pFast-hRad1
FEATURES             Location/Qualifiers
     source          1..5581
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  3..168
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      352..806
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(439..458)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     648..669
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        832..936
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(937..1005,1006..1797)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1155..1174)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1968..2556
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2457..2476
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2710..2727
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2861..3085
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(3152..3685)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3855..3860,3861..3877,3878..3883))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        4235..4326
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     4251..4268
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    5421..5555
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5458..5477)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5512..5531
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 cgaaccagat aagtgaaatc tagttccaaa ctattttgtc atttttaatt ttcgtattag
       61 cttacgacgc tacacccagt tcccatctat tttgtcactc ttccctaaat aatccttaaa
      121 aactccattt ccacccctcc cagttcccaa ctattttgtc cgcccacagc ggggcatttt
      181 tcttcctgtt atgtttttaa tcaaacatcc tgccaactcc atgtgacaaa ccgtcatctt
      241 cggctacttt ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt
      301 ttgtaattga ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct
      361 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
      421 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
      481 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
      541 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
      601 gatagacggt ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt
      661 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
      721 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
      781 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
      841 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
      901 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
      961 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     1021 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     1081 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     1141 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     1201 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     1261 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     1321 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     1381 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     1441 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     1501 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     1561 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     1621 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     1681 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     1741 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     1801 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     1861 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     1921 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     1981 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     2041 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     2101 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     2161 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     2221 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     2281 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     2341 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     2401 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     2461 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     2521 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     2581 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     2641 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     2701 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc
     2761 cttacgcatc tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg
     2821 gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc
     2881 ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt
     2941 tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct
     3001 aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc
     3061 caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg
     3121 ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa
     3181 agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac
     3241 cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga
     3301 gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat
     3361 agatatagat ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc
     3421 caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt
     3481 tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac
     3541 gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg
     3601 tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg
     3661 taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct
     3721 tggatgcccg aggcatagac tgtacaaaaa aacagtcata acaagccatg aaaaccgcca
     3781 ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac
     3841 gctacttgca ttacagttta cgaaccgaac aggcttatgt caactgggtt cgtgccttca
     3901 tccgtttcca cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc gaggcatttc
     3961 tgtcctggct ggcgaacgag cgcaaggttt cggtctccac gcatcgtcag gcattggcgg
     4021 ccttgctgtt cttctacggc aaggtgctgt gcacggatct gccctggctt caggagatcg
     4081 gaagacctcg gccgtcgcgg cgcttgccgg tggtgctgac cccggatgaa gtggttcgca
     4141 tcctcggttt tctggaaggc gagcatcgtt tgttcgccca ggactctagc tatagttcta
     4201 gtggttggct acgtatactc cggaatatta atagatcatg gagataatta aaatgataac
     4261 catctcgcaa ataaataagt attttactgt tttcgtaaca gttttgtaat aaaaaaacct
     4321 ataaatattc cggattattc ataccgtccc accatcgggc gcggatcccg gtccgaagcg
     4381 cgcggaattc aaaggcctac gtcgacacca tgccccttct gacccaacag atccaagacg
     4441 aggatgatca gtacagcctt gtggccagcc ttgacaacgt taggaatctc tccactatct
     4501 tgaaagctat tcatttccga gaacatgcca cgtgtttcgc aactaaaaat ggtatcaaag
     4561 taacagtgga aaatgcaaag tgtgtgcaag caaatgcttt tattcaggct ggaatatttc
     4621 aggagtttaa agttcaggaa gagtctgtta cttttcgaat taatttaact gtccttttag
     4681 actgtttatc tatttttgga tcaagtccta tgccagggac tttaactgca cttcgaatgt
     4741 gttaccaagg ttatggttac cctttgatgc tgttcctgga agaaggagga gtggtgacag
     4801 tctgcaaaat caatacacag gaacctgagg agaccctgga ctttgatttc tgcagcacca
     4861 atgttattaa taaaattatt ctgcagtcag aggggctccg tgaagcattt tctgaattgg
     4921 atatgacgag tgaagtccta caaattacca tgtctcctga caagccttat ttcaggttat
     4981 ctacttttgg aaatgcagga agttcccacc ttgactatcc caaagattct gatttgatgg
     5041 aagcatttca ttgtaatcag acccaagtca acagatacaa gatttcctta ctgaaaccct
     5101 ctacaaaggc attagtccta tcttgtaagg tatctattcg gacagataac agaggcttcc
     5161 tttcattaca gtatatgatt agaaatgaag atggacaaat atgttttgtg gaatattact
     5221 gctgccctga tgaagaagtt cctgaatctg agtcttgatc tagagcctgc agtctcgagg
     5281 catgcggtac caagcttgtc gagaagtact agaggatcat aatcagccat accacatttg
     5341 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     5401 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     5461 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     5521 ccaaactcat caatgtatct tatcatgtct ggatctgatc actgcttgag cctaggagat
     5581 c
//
