LOCUS       sequence-456184-        5594 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses hHus1 in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   Lindsey-Boltz LA, Bermudez VP, Hurwitz J, Sancar A
  TITLE     Purification and characterization of human DNA damage checkpoint Rad
            complexes.
  JOURNAL   Proc Natl Acad Sci U S A. 2001 Sep 25;98(20):11236-41. doi: 
            10.1073/pnas.201373498.
  PUBMED    11572977
REFERENCE   2  (bases 1 to 5594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1073/pnas"; 
            journalName: "Proc Natl Acad Sci U S A"; date: "2001-09-25- 25"; 
            volume: "98"; issue: "20"; pages: "11236-41"
COMMENT     Sequence Label: pFast-hHus1
FEATURES             Location/Qualifiers
     source          1..5594
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  57..222
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      406..860
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(493..512)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     702..723
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        886..990
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(991..1059,1060..1851)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1209..1228)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2022..2610
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2511..2530
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2764..2781
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2915..3139
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(3206..3739)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3928..3933,3934..3950,3951..3956))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        4308..4399
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     4324..4341
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    join(5488..5594,1..28)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5525..5544)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     join(5579..5594,1..4)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 catcaatgta tcttatcatg tctggatctg atcactgctt gagcctagga gatccgaacc
       61 agataagtga aatctagttc caaactattt tgtcattttt aattttcgta ttagcttacg
      121 acgctacacc cagttcccat ctattttgtc actcttccct aaataatcct taaaaactcc
      181 atttccaccc ctcccagttc ccaactattt tgtccgccca cagcggggca tttttcttcc
      241 tgttatgttt ttaatcaaac atcctgccaa ctccatgtga caaaccgtca tcttcggcta
      301 ctttttctct gtcacagaat gaaaattttt ctgtcatctc ttcgttatta atgtttgtaa
      361 ttgactgaat atcaacgctt atttgcagcc tgaatggcga atgggacgcg ccctgtagcg
      421 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
      481 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
      541 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
      601 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
      661 cggtttttcg ccctttgacg ttggagtcca cgttcttaat agtggactct tgttccaaac
      721 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
      781 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
      841 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
      901 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
      961 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     1021 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     1081 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     1141 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     1201 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     1261 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     1321 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     1381 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     1441 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     1501 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     1561 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     1621 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     1681 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     1741 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     1801 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     1861 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     1921 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     1981 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2041 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2101 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2161 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2221 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2281 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2341 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2401 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2461 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2521 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2581 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     2641 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     2701 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     2761 cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg
     2821 catctgtgcg gtatttcaca ccgcatagac cagccgcgta acctggcaaa atcggttacg
     2881 gttgagtaat aaatggatgc cctgcgtaag cgggtgtggg cggacaataa agtcttaaac
     2941 tgaacaaaat agatctaaac tatgacaata aagtcttaaa ctagacagaa tagttgtaaa
     3001 ctgaaatcag tccagttatg ctgtgaaaaa gcatactgga cttttgttat ggctaaagca
     3061 aactcttcat tttctgaagt gcaaattgcc cgtcgtatta aagaggggcg tggccaaggg
     3121 catggtaaag actatattcg cggcgttgtg acaatttacc gaacaactcc gcggccggga
     3181 agccgatctc ggcttgaacg aattgttagg tggcggtact tgggtcgata tcaaagtgca
     3241 tcacttcttc ccgtatgccc aactttgtat agagagccac tgcgggatcg tcaccgtaat
     3301 ctgcttgcac gtagatcaca taagcaccaa gcgcgttggc ctcatgcttg aggagattga
     3361 tgagcgcggt ggcaatgccc tgcctccggt gctcgccgga gactgcgaga tcatagatat
     3421 agatctcact acgcggctgc tcaaacttgg gcagaacgta agccgcgaga gcgccaacaa
     3481 ccgcttcttg gtcgaaggca gcaagcgcga tgaatgtctt actacggagc aagttcccga
     3541 ggtaatcgga gtccggctga tgttgggagt aggtggctac gtctccgaac tcacgaccga
     3601 aaagatcaag agcagcccgc atggatttga cttggtcagg gccgagccta catgtgcgaa
     3661 tgatgcccat acttgagcca cctaactttg ttttagggcg actgccctgc tgcgtaacat
     3721 cgttgctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc cacggcgtaa
     3781 cgcgcttgct gcttggatgc ccgaggcata gactgtacaa aaaaacagtc ataacaagcc
     3841 atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg
     3901 cgtgagcgca tacgctactt gcattacagt ttacgaaccg aacaggctta tgtcaactgg
     3961 gttcgtgcct tcatccgttt ccacggtgtg cgtcacccgg caaccttggg cagcagcgaa
     4021 gtcgaggcat ttctgtcctg gctggcgaac gagcgcaagg tttcggtctc cacgcatcgt
     4081 caggcattgg cggccttgct gttcttctac ggcaaggtgc tgtgcacgga tctgccctgg
     4141 cttcaggaga tcggaagacc tcggccgtcg cggcgcttgc cggtggtgct gaccccggat
     4201 gaagtggttc gcatcctcgg ttttctggaa ggcgagcatc gtttgttcgc ccaggactct
     4261 agctatagtt ctagtggttg gctacgtata ctccggaata ttaatagatc atggagataa
     4321 ttaaaatgat aaccatctcg caaataaata agtattttac tgttttcgta acagttttgt
     4381 aataaaaaaa cctataaata ttccggatta ttcataccgt cccaccatcg ggcgcggatc
     4441 ccggtccgaa gcgcgcggaa ttcaaaggcc tacgtcgaca ccatgaagtt tcgggccaag
     4501 atcgtggacg gggcctgtct gaaccacttc acacgaatca gtaacatgat agccaagctt
     4561 gccaaaacct gcaccctccg catcagccct gataagctta acttcatcct ttgtgacaag
     4621 ctggctaatg gaggagtgag catgtggtgt gagctggaac aggagaactt cttcaacgaa
     4681 tttcaaatgg agggtgtctc tgcagaaaac aatgagattt atttagagct aacatcggaa
     4741 aacttatctc gagccttgaa gactgcccag aatgccaggg ctttgaaaat caaactgact
     4801 aataaacact ttccctgcct cacggtctcc gtggagctgt tatctatgtc aagcagtagc
     4861 cgcattgtga cccatgacat ccccataaag gtgattccta ggaaattgtg gaaggactta
     4921 caagaaccgg ttgtcccaga tcctgatgtt agtatttatt taccagtctt gaagactatg
     4981 aagagtgttg tggaaaaaat gaaaaacatc agcaatcacc ttgttattga agcaaaccta
     5041 gatggagaat tgaatttgaa aatagaaact gaattagtat gtgttacaac tcattttaaa
     5101 gatcttggaa atcctccatt agcctctgaa agcacccatg aggacagaaa cgtggaacac
     5161 atggctgaag tgcacataga tattaggaag ctcctacagt ttcttgctgg acaacaagta
     5221 aatcccacaa aggccttatg caatattgtg aataacaaga tggtgcattt tgatctgctt
     5281 catgaagacg tgtcccttca gtatttcatc cctgcgctgt cctagtctag agcctgcagt
     5341 ctcgaggcat gcggtaccaa gcttgtcgag aagtactaga ggatcataat cagccatacc
     5401 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     5461 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     5521 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     5581 ggtttgtcca aact
//
