LOCUS       sequence-456210-        2019 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Recombination to BV DNA (Tn7).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Lai Z, Flanigan SF, Boudes M, Davidovich C
  TITLE     Modular cloning of multigene vectors for the baculovirus system and 
            yeast.
  JOURNAL   J Mol Biol. 2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943.
  PUBMED    39814169
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943."
COMMENT     Sequence Label: pMB.003
FEATURES             Location/Qualifiers
     source          1..2019
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  16..181
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     terminator      complement(384..418)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(489..1148)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1063..1082
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1330..1918
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1819..1838
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat acatcaacca gataagtgaa atctagttcc aaactatttt gtcattttta
       61 attttcgtat tagcttacga cgctacaccc agttcccatc tattttgtca ctcttcccta
      121 aataatcctt aaaaactcca tttccacccc tcccagttcc caactatttt gtccgcccac
      181 agcggggcat ttttcttcct gttatgtttt taatcaaaca tcctgccaac tccatgtgac
      241 aaaccgtcat cttcggctac tttttctctg tcacagaatg aaaatttttc tgtcatctct
      301 tcgttattaa tgtttgtaat tgactgaata tcaacgctta tttgcagcct gaatggcgaa
      361 tggggggagt tgagaccaga ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca
      421 aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg
      481 atatcaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
      541 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
      601 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
      661 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgaaacgaaa
      721 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
      781 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
      841 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
      901 accagctcac cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca
      961 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     1021 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     1081 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     1141 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     1201 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgcccgat caatcatgac
     1261 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1321 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1381 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1441 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     1501 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1561 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1621 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1681 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     1741 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     1801 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     1861 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     1921 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     1981 ctttcctgcg ttatcccctg attctgtgga taaccgtag
//
