LOCUS       sequence-456214-        1834 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  polH Promoter for Baculovirus expression without Nterminal tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Lai Z, Flanigan SF, Boudes M, Davidovich C
  TITLE     Modular cloning of multigene vectors for the baculovirus system and 
            yeast.
  JOURNAL   J Mol Biol. 2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943.
  PUBMED    39814169
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943."
COMMENT     Sequence Label: pMB.005
FEATURES             Location/Qualifiers
     source          1..1834
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        49..140
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     65..82
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     terminator      complement(199..233)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(304..963)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     878..897
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1145..1733
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1634..1653
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acgctagtgg ttggctacat actccggaat attaatagat catggagata
       61 attaaaatga taaccatctc gcaaataaat aagtatttta ctgttttcgt aacagttttg
      121 taataaaaaa acctataaat attccggatt attcataccg tcccaccatc gggcgctccg
      181 gtatgtgaga ccagaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc
      241 agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc
      301 aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc
      361 gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt
      421 gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt
      481 ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaaa cgaaaaacat
      541 attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg
      601 cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa
      661 cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag
      721 ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat
      781 gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt
      841 aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa
      901 atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc
      961 cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga
     1021 tcttatttca ttatggtgaa agttggaacc tcttacgtgc ccgatcaatc atgaccaaaa
     1081 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1141 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1201 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     1261 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     1321 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1381 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     1441 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     1501 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     1561 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     1621 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     1681 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     1741 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     1801 ctgcgttatc ccctgattct gtggataacc gtag
//
