LOCUS       sequence-456216-        1831 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  polH Promoter for Baculovirus expression with Nterminal tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Lai Z, Flanigan SF, Boudes M, Davidovich C
  TITLE     Modular cloning of multigene vectors for the baculovirus system and 
            yeast.
  JOURNAL   J Mol Biol. 2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943.
  PUBMED    39814169
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943."
COMMENT     Sequence Label: pMB.006
FEATURES             Location/Qualifiers
     source          1..1831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        49..140
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     65..82
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     terminator      complement(196..230)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(301..960)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     875..894
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1142..1730
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1631..1650
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acgctagtgg ttggctacat actccggaat attaatagat catggagata
       61 attaaaatga taaccatctc gcaaataaat aagtatttta ctgttttcgt aacagttttg
      121 taataaaaaa acctataaat attccggatt attcataccg tcccaccatc gggcgcggaa
      181 gatgagacca gaccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga
      241 tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa
      301 ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac
      361 atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc
      421 gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc
      481 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgaaacga aaaacatatt
      541 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga
      601 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt
      661 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc
      721 accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg
      781 aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat
      841 atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg
      901 ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat
      961 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
     1021 tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaatcatg accaaaatcc
     1081 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     1141 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     1201 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     1261 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     1321 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     1381 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     1441 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     1501 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     1561 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     1621 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     1681 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     1741 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     1801 cgttatcccc tgattctgtg gataaccgta g
//
