LOCUS       sequence-456242-       10272 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CROP-seq-puro-v2 with scaffold sequence removed for the insertion of
            CRISPRmap Opool with guide, scaffold and barcode sequences, using 
            copGFP as selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10272)
  AUTHORS   Gu J, Iyer A, Wesley B, Taglialatela A, Leuzzi G, Hangai S, Decker 
            A, Gu R, Klickstein N, Shuai Y, Jankovic K, Parker-Burns L, Jin Y, 
            Zhang JY, Hong J, Niu X, Costa JA, Pezet MG, Chou J, Snoeck HW, 
            Landau DA, Azizi E, Chan EM, Ciccia A, Gaublomme JT
  TITLE     Mapping multimodal phenotypes to perturbations in cells and tissue 
            with CRISPRmap.
  JOURNAL   Nat Biotechnol. 2024 Oct 7. doi: 10.1038/s41587-024-02386-x.
  PUBMED    39375448
REFERENCE   2  (bases 1 to 10272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Oct 7. doi: 10.1038/s41587-024-02386-x."
COMMENT     Sequence Label: CROP-seq-copGFP-v2-no-scaffold
FEATURES             Location/Qualifiers
     source          1..10272
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1489..1606
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1745..2923
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1976..2914
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2871..2891
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             2949..3611
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     misc_feature    3691..4279
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3744..3764)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4162..4173)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4435..4683
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4435..4455
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4606..4625
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     LTR             6611..6791
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6869..6990
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6906..6925)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6960..6979
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7030..7165
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7185..7204)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7186..7204)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7214..7231)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7214..7230)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7223..7245)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7372..7827
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7459..7478)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7669..7690
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7853..7957
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7958..8026,8027..8818)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8176..8195)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8989..9577
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9478..9497
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9731..9748
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9865..9886
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9901..9906,9907..9924,9925..9931)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9939..9955
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9944..9966
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9963..9979
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9963..9979
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9998..10018
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10000..10018
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10046..10272
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaatct tgagacaaat ggcagtattc atccacaatt ttaaaagaaa
     1501 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     1561 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     1621 ggacagcaga gatccacttt ggcgccggct cgagggggcc cgggtgcaaa gatggataaa
     1681 gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg
     1741 aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     1801 ggggggaggg gtcggcaatt gatccggtgc ctagagaagg tggcgcgggg taaactggga
     1861 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     1921 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
     1981 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
     2041 aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg
     2101 gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc
     2161 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
     2221 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
     2281 ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg
     2341 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
     2401 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
     2461 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
     2521 accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg
     2581 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
     2641 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
     2701 cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
     2761 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
     2821 gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca
     2881 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgacatggag agcgacgaga
     2941 gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt
     3001 tcgagctggt gggcggcgga gagggcaccc ccaagcaggg ccgcatgacc aacaagatga
     3061 agagcaccaa aggcgccctg accttcagcc cctacctgct gagccacgtg atgggctacg
     3121 gcttctacca cttcggcacc taccccagcg gctacgagaa ccccttcctg cacgccatca
     3181 acaacggcgg ctacaccaac acccgcatcg agaagtacga ggacggcggc gtgctgcacg
     3241 tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca
     3301 ccggcttccc cgaggacagc gtgatcttca ccgacaagat catccgcagc aacgccaccg
     3361 tggagcacct gcaccccatg ggcgataacg tgctggtggg cagcttcgcc cgcaccttca
     3421 gcctgcgcga cggcggctac tacagcttcg tggtggacag ccacatgcac ttcaagagcg
     3481 ccatccaccc cagcatcctg cagaacgggg gccccatgtt cgccttccgc cgcgtggagg
     3541 agctgcacag caacaccgag ctgggcatcg tggagtacca gcacgccttc aagaccccca
     3601 tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc cgtggacggc accgccggac
     3661 ccggctccac cggatctcgc ttaagtcgac aatcaacctc tggattacaa aatttgtgaa
     3721 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     3781 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     3841 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     3901 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     3961 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     4021 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     4081 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     4141 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     4201 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     4261 ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc
     4321 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     4381 aagatttcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cagagagggc
     4441 ctatttccca tgattccttc atatttgcat atacgataca aggctgttag agagataatt
     4501 agaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa
     4561 taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt
     4621 accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac
     4681 accggagacg gttgtaaatg agcacacaaa atacacatgc taaaatatta tattctatga
     4741 cctttataaa atcaaccaaa atcttctttt taataacttt agtatcaata attagaattt
     4801 ttatgttcct ttttgcaaac ttttaataaa aatgagcaaa ataaaaaaac gctagtttta
     4861 gtaactcgcg ttgttttctt cacctttaat aatagctact ccaccacttg ttcctaagcg
     4921 gtcagctcct gcttcaatca ttttttgagc atcttcaaat gttctaactc caccagctgc
     4981 tttaactaaa gcattgtctt taacaactga cttcattagt ttaacatctt caaatgttgc
     5041 acctgatttt gaaaatcctg ttgatgtttt aacaaattct aatccagctt caacagctat
     5101 ttcacaagct ttcatgattt cttcttttgt taataaacaa ttttccataa tacatttaac
     5161 aacatgtgat ccagctgctt tttttacagc tttcatgtct tctaaaacta attcataatt
     5221 tttgtctttt aatgcaccaa tatttaatac catatcaatt tctgttgcac catctttaat
     5281 tgcttcagaa acttcgaatg cttttgtagc tgttgtgcat gcacctagag gaaaacctac
     5341 aacatttgtt attcctacat ttgtgccttt taataattct ttacaatagc ttgttcaata
     5401 tgaattaaca caaactgttg caaaatcaaa ttcaattgct tcatcacata attgtttaat
     5461 ttcagctttc gtagcatctt gttttaataa tgtgtgatct atatatttgt ttagtttcat
     5521 tttttctcct atatattcat ttttaatttt aattctttaa taatttcgtc tactttaact
     5581 ttagcgtttt gaacagattc accaacacct ataaaataaa tttttagttt aggttcagtt
     5641 ccacttgggc gaacagcaaa tcatgactta tcttctaaat aaaattttag taagtcttgt
     5701 cctggcatat tatacattcc atcgatgtag tcttcaacat taacaacttt aagtccagca
     5761 atttgagtta agggtgttgc tctcaatgat ttcattaatg gttcaatttt taatttcttt
     5821 tcttctggtt taaaattcaa gtttaaagtg aaagtgtaat atgcacccat ttctttaaat
     5881 aaatcttcta aatagtctac taatgtttta ttttgttttt tataaaatca agcagcctct
     5941 gctattaata tagaagcttg tattccatct ttatctctag ctgagtcatc aattacatat
     6001 ccataacttt cttcataagc aaaaacaaaa tttaatccgt tatcttcttc tttagcaatt
     6061 tctctaccca ttcatttaaa tccagttaaa gtttttacaa tattaactcc atatttttca
     6121 tgagcgattc tatcacccaa atcacttgtt acaaaacttg aatatagagc cggatttttt
     6181 ggaatgctat ttaagcgttt tagatttgat aattttcaat caattaaaat tggtcctgtt
     6241 tgatttccat ctaatcttac aaaatgacca tcatgtttta ttgccattcc aaatctgtca
     6301 gcatctgggt cattcataat aataatatct gcatcatgtt taataccata ttcaagcggt
     6361 atttttcatg caggatcaaa ttctggattt ggatttacaa catttttaaa tgtttcatct
     6421 tcaaatgcat gctcttcaac ctcaataacg ttatatcctg attcacgtaa tatttttggg
     6481 gtaaatttag ttcctgttcc attaactgcg ctaaaaataa tttttaaatc ttttttagct
     6541 tcttgctctt ttttgtacgt ctctaagctt ggcgtaacta gatcttgaga cactgctttt
     6601 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     6661 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     6721 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     6781 aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt tataacttgc
     6841 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     6901 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     6961 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     7021 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     7081 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     7141 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt
     7201 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     7261 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     7321 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
     7381 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     7441 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     7501 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     7561 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     7621 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     7681 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     7741 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     7801 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
     7861 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     7921 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     7981 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     8041 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     8101 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     8161 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     8221 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     8281 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     8341 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     8401 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     8461 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     8521 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     8581 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     8641 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     8701 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     8761 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     8821 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     8881 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     8941 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     9001 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     9061 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     9121 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     9181 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     9241 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     9301 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     9361 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     9421 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     9481 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     9541 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     9601 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     9661 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     9721 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     9781 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     9841 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     9901 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     9961 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
    10021 acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac
    10081 atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga
    10141 ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca
    10201 tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct
    10261 cgatacataa ac
//
