LOCUS       sequence-456243-       10132 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CROP-seq-puro-v2 with scaffold sequence removed for the insertion of
            CRISPRmap Opool with guide, scaffold and barcode sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10132)
  AUTHORS   Gu J, Iyer A, Wesley B, Taglialatela A, Leuzzi G, Hangai S, Decker 
            A, Gu R, Klickstein N, Shuai Y, Jankovic K, Parker-Burns L, Jin Y, 
            Zhang JY, Hong J, Niu X, Costa JA, Pezet MG, Chou J, Snoeck HW, 
            Landau DA, Azizi E, Chan EM, Ciccia A, Gaublomme JT
  TITLE     Mapping multimodal phenotypes to perturbations in cells and tissue 
            with CRISPRmap.
  JOURNAL   Nat Biotechnol. 2024 Oct 7. doi: 10.1038/s41587-024-02386-x.
  PUBMED    39375448
REFERENCE   2  (bases 1 to 10132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Oct 7. doi: 10.1038/s41587-024-02386-x."
COMMENT     Sequence Label: CROP-seq-puro-v2-no-scaffold
FEATURES             Location/Qualifiers
     source          1..10132
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1222..1402
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    1480..1601
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1517..1536)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1571..1590
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1641..1776
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1796..1815)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1797..1815)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1825..1842)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1825..1841)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1834..1856)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1983..2438
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2070..2089)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2280..2301
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2464..2568
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2569..2637,2638..3429)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2787..2806)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3600..4188
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4089..4108
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4342..4359
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4476..4497
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4512..4517,4518..4535,4536..4542)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4550..4566
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4555..4577
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4574..4590
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4574..4590
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4609..4629
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4611..4629
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4657..4883
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4884..5064
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5111..5236
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5729..5962
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6147..6191
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6372..6489
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6628..7806
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6859..7797
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7754..7774
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             7819..8418
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7819..7838)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8315..8335
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8434..9022
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8487..8507)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8905..8916)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        9178..9426
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     9178..9198
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     9349..9368
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 cttgttcaat atgaattaac acaaactgtt gcaaaatcaa attcaattgc ttcatcacat
       61 aattgtttaa tttcagcttt cgtagcatct tgttttaata atgtgtgatc tatatatttg
      121 tttagtttca ttttttctcc tatatattca tttttaattt taattcttta ataatttcgt
      181 ctactttaac tttagcgttt tgaacagatt caccaacacc tataaaataa atttttagtt
      241 taggttcagt tccacttggg cgaacagcaa atcatgactt atcttctaaa taaaatttta
      301 gtaagtcttg tcctggcata ttatacattc catcgatgta gtcttcaaca ttaacaactt
      361 taagtccagc aatttgagtt aagggtgttg ctctcaatga tttcattaat ggttcaattt
      421 ttaatttctt ttcttctggt ttaaaattca agtttaaagt gaaagtgtaa tatgcaccca
      481 tttctttaaa taaatcttct aaatagtcta ctaatgtttt attttgtttt ttataaaatc
      541 aagcagcctc tgctattaat atagaagctt gtattccatc tttatctcta gctgagtcat
      601 caattacata tccataactt tcttcataag caaaaacaaa atttaatccg ttatcttctt
      661 ctttagcaat ttctctaccc attcatttaa atccagttaa agtttttaca atattaactc
      721 catatttttc atgagcgatt ctatcaccca aatcacttgt tacaaaactt gaatatagag
      781 ccggattttt tggaatgcta tttaagcgtt ttagatttga taattttcaa tcaattaaaa
      841 ttggtcctgt ttgatttcca tctaatctta caaaatgacc atcatgtttt attgccattc
      901 caaatctgtc agcatctggg tcattcataa taataatatc tgcatcatgt ttaataccat
      961 attcaagcgg tatttttcat gcaggatcaa attctggatt tggatttaca acatttttaa
     1021 atgtttcatc ttcaaatgca tgctcttcaa cctcaataac gttatatcct gattcacgta
     1081 atatttttgg ggtaaattta gttcctgttc cattaactgc gctaaaaata atttttaaat
     1141 cttttttagc ttcttgctct tttttgtacg tctctaagct tggcgtaact agatcttgag
     1201 acactgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     1261 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     1321 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     1381 tcagtgtgga aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat
     1441 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     1501 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     1561 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     1621 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     1681 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     1741 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
     1801 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     1861 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     1921 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     1981 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     2041 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     2101 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     2161 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     2221 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     2281 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     2341 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     2401 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     2461 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     2521 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     2581 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     2641 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     2701 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     2761 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     2821 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     2881 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     2941 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     3001 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     3061 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     3121 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     3181 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     3241 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     3301 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     3361 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     3421 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     3481 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     3541 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3601 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3661 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3721 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3781 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3841 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3901 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3961 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     4021 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     4081 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     4141 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     4201 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     4261 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     4321 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     4381 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     4441 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     4501 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     4561 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
     4621 cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt
     4681 agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc
     4741 gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga
     4801 cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta
     4861 agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc
     4921 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     4981 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     5041 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     5101 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     5161 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     5221 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     5281 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     5341 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     5401 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     5461 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     5521 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     5581 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     5641 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     5701 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     5761 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     5821 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     5881 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     5941 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     6001 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     6061 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     6121 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     6181 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     6241 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     6301 atagagttag gcagggatat tcaccattat cgtttcagaa tcttgagaca aatggcagta
     6361 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     6421 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     6481 caaaattttc gggtttatta cagggacagc agagatccac tttggcgccg gctcgagggg
     6541 gcccgggtgc aaagatggat aaagttttaa acagagagga atctttgcag ctaatggacc
     6601 ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca
     6661 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgatccgg tgcctagaga
     6721 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
     6781 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
     6841 tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg
     6901 ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat
     6961 cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct
     7021 tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg
     7081 tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga
     7141 tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg
     7201 cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc
     7261 acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct
     7321 caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg
     7381 gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc
     7441 cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca
     7501 cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag
     7561 taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta
     7621 ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa
     7681 gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga
     7741 tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg
     7801 tcgtgacgta cggccaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     7861 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     7921 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     7981 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgccgtggcg
     8041 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     8101 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     8161 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggagtctc gcccgaccac
     8221 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     8281 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     8341 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     8401 cgcaagcccg gtgcctgaac gcgttaagtc gacaatcaac ctctggatta caaaatttgt
     8461 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     8521 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     8581 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     8641 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     8701 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     8761 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     8821 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     8881 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     8941 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     9001 tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag
     9061 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     9121 gacaagattt caccattatc gtttcagacc cacctcccaa ccccgagggg acccagagag
     9181 ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata
     9241 attagaatta atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag
     9301 taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg
     9361 cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
     9421 aacaccggag acggttgtaa atgagcacac aaaatacaca tgctaaaata ttatattcta
     9481 tgacctttat aaaatcaacc aaaatcttct ttttaataac tttagtatca ataattagaa
     9541 tttttatgtt cctttttgca aacttttaat aaaaatgagc aaaataaaaa aacgctagtt
     9601 ttagtaactc gcgttgtttt cttcaccttt aataatagct actccaccac ttgttcctaa
     9661 gcggtcagct cctgcttcaa tcattttttg agcatcttca aatgttctaa ctccaccagc
     9721 tgctttaact aaagcattgt ctttaacaac tgacttcatt agtttaacat cttcaaatgt
     9781 tgcacctgat tttgaaaatc ctgttgatgt tttaacaaat tctaatccag cttcaacagc
     9841 tatttcacaa gctttcatga tttcttcttt tgttaataaa caattttcca taatacattt
     9901 aacaacatgt gatccagctg ctttttttac agctttcatg tcttctaaaa ctaattcata
     9961 atttttgtct tttaatgcac caatatttaa taccatatca atttctgttg caccatcttt
    10021 aattgcttca gaaacttcga atgcttttgt agctgttgtg catgcaccta gaggaaaacc
    10081 tacaacattt gttattccta catttgtgcc ttttaataat tctttacaat ag
//
