LOCUS       sequence-456245-       10254 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for the expression of MpRTN1 fused to the C-terminal half of 
            YFP in plants (for Agrobacterium-mediated genetic transformation.).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10254)
  AUTHORS   Ishikawa K, Konno R, Hirano S, Fujii Y, Fujiwara M, Fukao Y, Kodama 
            Y
  TITLE     The ER membrane-bending protein RETICULON facilitates chloroplast 
            relocation movement in Marchantia polymorpha.
  JOURNAL   Plant J. 2022 Apr 27. doi: 10.1111/tpj.15787.
  PUBMED    35476214
REFERENCE   2  (bases 1 to 10254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2022 Apr 27. doi: 10.1111/tpj.15787."
COMMENT     Sequence Label: pB4GWcY-MpRTN1
FEATURES             Location/Qualifiers
     source          1..10254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(62..83)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    228..252
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(985..1009)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(1031..1376)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(1068..1088)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     complement(1891..1908)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1891..1907)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1900..1922)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    2110..2134
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3435..4064
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4493..5566
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      5632..5826
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6062..6084)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6170..6310
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6225..6244
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6325..6342)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6496..7084)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6576..6595)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7330..8121)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    8642..8666
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        8840..9019
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        8872..9055
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             9076..9870
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9130..9149)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9740..9759
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 aattcgagct ctaagcttac ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg
       61 tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg
      121 actggtagct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt
      181 tctgctggta gtggtcggcg agctgcacgc tgcccatgct gttatcaacc actttgtaca
      241 agaaagctgg gtcctgcgcc ttcttgtcct tgggcgcagc cctagggatt ttactcagca
      301 ggagagcatc gatctttttg tactggacat tagcctgatc aactgcaacc ttgccgtact
      361 tgtcaacttc atcttcgtac ttctcataca gagctgggac cgtatgggag gcgacaacac
      421 cgatgtacac aagtgtcaag aagttgaacc atccaccaat aacagagagc acccagaggc
      481 cggcaaccac cttcagaaag agcttccaat cccttccgag agcaacatcg tgtgctattg
      541 caaggatttt gttgatttca acgcggagag tttttgcagt gtgaaccacg gtgtcttcag
      601 atagcgcaag ttcaggtagt ggaggtggag ctctgttcag aagaccagca gcattcgacc
      661 acaagaagaa gatgacaatg aggaagagaa gcacgttcga gacgagggac agtagagtgt
      721 aaccagacca ctcgaataga atccaaccga ttgtcgctcc cgctagaaca ccaccggaca
      781 aaggcttgtg cttccacaaa aggacgtccg cagctttgcc accgccaagt agctgatgaa
      841 ctgtcttttc cctgttaaat agccggccat gtgaagattt gcccacggca tgtgatgatg
      901 tcgccgcggg gagcttcgac aggttttcca ccacgttgtt tacagcggcc gccactgctc
      961 cttccgccac atgttcagac atgaagcctg cttttttgta caaacttgtt gataactcta
     1021 gagtcccccg tgttctctcc aaatgaaatg aacttcctta tatagaggaa gggtcttgcg
     1081 aaggatagtg ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt
     1141 gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc
     1201 catctttggg accactgtcg gcagaggcat cttcaacgat ggcctttcct ttatcgcaat
     1261 gatggcattt gtaggagcca ccttcctttt ccactatctt cacaataaag tgacagatag
     1321 ctgggcaatg gaatccgagg aggtttccgg atattaccct ttgttgaaaa gtctcaattg
     1381 ccctttggtc ttctgagact gtatctttga tatttttgga gtagacaagt gtgtcgtgct
     1441 ccaccatgtt gacgaagatt ttcttcttgt cattgagtcg taagagactc tgtatgaact
     1501 gttcgccagt ctttacggcg agttctgtta ggtcctctat ttgaatcttt gactccatgg
     1561 cctttgattc agtgggaact acctttttag agactccaat ctctattact tgccttggtt
     1621 tgtgaagcaa gccttgaatc gtccatactg gaatagtact tctgatcttg agaaatatat
     1681 ctttctctgt gttcttgatg cagttagtcc tgaatctttt gactgcatct ttaaccttct
     1741 tgggaaggta tttgatttcc tggagattat tgctcgggta gatcgtcttg atgagacctg
     1801 ctgcgtaagc ctctctaacc atctgtgggt tagcattctt tctgaaattg aaaaggctaa
     1861 tctggggacc tgcaggcatg caagcttggc actggccgtc gttttacaac gtcgtgactg
     1921 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     1981 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     2041 cgaatgctag agcagcttga gcttggatca gattgtcgtt tcccgccttc agtttaaact
     2101 atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa
     2161 taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg
     2221 ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc gctgctatag
     2281 tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt cgcacaagtc
     2341 ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt
     2401 tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca tgaacaagag
     2461 cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg acttgaccaa
     2521 ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga agatcaccgg
     2581 caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc ctggcgacgt
     2641 tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac tggacattgc
     2701 cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt gggccgacac
     2761 caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg
     2821 ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa
     2881 gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg agctgatcga
     2941 ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct
     3001 gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc
     3061 cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga atgaacgcca
     3121 agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt cattaccgaa
     3181 gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc gcacgtctca
     3241 accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc ggcctggccg
     3301 gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag
     3361 taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata
     3421 cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg tcaggcaaga
     3481 cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc cggggccgat gttctgttag
     3541 tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc
     3601 taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc
     3661 gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg tccgcgatca
     3721 aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata tgggccaccg
     3781 ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg
     3841 cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc
     3901 tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg agctacccag
     3961 gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac gctgcccgcg
     4021 aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat gaggtaaaga
     4081 gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac gcagcagcaa
     4141 ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca actttcagtt
     4201 gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca agaccattac
     4261 cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat gaataaatga
     4321 gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg
     4381 acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta agcggctggg
     4441 ttgtctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg gcgtgagcgg
     4501 tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa
     4561 gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac gccccggtga
     4621 atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc cggcagccgg
     4681 tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt tcgttccgat
     4741 gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg ttttccgtct
     4801 gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag acgggcacgt
     4861 agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc tggtactgat
     4921 ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg gagacaagcc
     4981 cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc gagccgatgg
     5041 cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc
     5101 catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg agggtgaagc
     5161 cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt acatcgagat
     5221 cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg acgtgctgac
     5281 ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct accgcctggc
     5341 acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct acgaacgcag
     5401 tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa
     5461 tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga tcctagtcat
     5521 gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta cggagcagat
     5581 gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggtctctttc ctgtggatag
     5641 cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca ttgggaaccc
     5701 aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag agaaaaaagg
     5761 cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc gcctggcctg
     5821 tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta cccttcggtc
     5881 gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa
     5941 aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc cgtcgccact
     6001 cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat gacggtgaaa
     6061 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     6121 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
     6181 cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat
     6241 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     6301 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     6361 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     6421 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     6481 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     6541 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     6601 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     6661 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     6721 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     6781 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     6841 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     6901 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     6961 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7021 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7081 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7141 ttaagggatt ttggtcatgc atgatatatc tcccaatttg tgtagggctt attatgcacg
     7201 cttaaaaata ataaaagcag acttgacctg atagtttggc tgtgagcaat tatgtgctta
     7261 gtgcatctaa cgcttgagtt aagccgcgcc gcgaagcggc gtcggcttga acgaatttct
     7321 agctagacat tatttgccga ctaccttggt gatctcgcct ttcacgtagt ggacaaattc
     7381 ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat aagcctgtct
     7441 agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc agtcggcagc
     7501 gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg acaacgtaag
     7561 cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg ttaaggtttc
     7621 atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct ccgccgctgg
     7681 acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca gatcaatgtc
     7741 gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt ctccaaattg
     7801 cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac
     7861 ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt
     7921 gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca gcaaatcaat
     7981 atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta cggccagcaa
     8041 cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag tcgatacttc
     8101 ggcgatcacc gcttccccca tgatgtttaa ctttgtttta gggcgactgc cctgctgcgt
     8161 aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt
     8221 ggatgcccga ggcatagact gtaccccaaa aaaacagtca taacaagcca tgaaaaccgc
     8281 cactgcgccg ttaccaccgc tgcgttcggt caaggttctg gaccagttgc gtgagcgcat
     8341 acgctacttg cattacagct tacgaaccga acaggcttat gtccactggg ttcgtgcccg
     8401 aattgatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag
     8461 atcatccgtg tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca
     8521 tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc
     8581 tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg
     8641 gtggcaggat atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc
     8701 ggacgttttt aatgtactga attaacgccg aattaggctg aatttatttc tgaattcatc
     8761 atgagcggag aattaaggga gtcacgttat gacccccgcc gatgacgcgg gacaagccgt
     8821 tttacgtttg gaactgacag aaccgcaacg ttgaaggagc cactcagccg cgggtttctg
     8881 gagtttaatg agctaagcac atacgtcaga aaccattatt gcgcgttcaa aagtcgccta
     8941 aggtcactat cagctagcaa atatttcttg tcaaaaatgc tccactgacg ttccataaat
     9001 tcccctcggt atccaattag agtctcatat tcactctcaa tccaaataat ctgcaccgga
     9061 tctggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     9121 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     9181 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     9241 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     9301 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     9361 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     9421 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     9481 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     9541 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     9601 gcgcgcatgc ccgacggcga tgatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     9661 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     9721 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     9781 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     9841 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
     9901 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     9961 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
    10021 tgctggagtt cttcgcccac gggatctctg cggaacaggc ggtcgaaggt gccgatatca
    10081 ttacgacagc aacggccgac aagcacaacg ccacgatcct gagcgacaat atgatcgggc
    10141 ccggcgtcca catcaacggc gtcggcggcg actgcccagg caagaccgag atgcaccgcg
    10201 atatcttgct gcgttcggat attttcgtgg agttcccgcc acagacccgg atga
//
