LOCUS       sequence-456247-       10578 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for the expression of MpRTN1 fused to the N-terminal half of 
            YFP in plants (for Agrobacterium-mediated genetic transformation.).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10578)
  AUTHORS   Ishikawa K, Konno R, Hirano S, Fujii Y, Fujiwara M, Fukao Y, Kodama 
            Y
  TITLE     The ER membrane-bending protein RETICULON facilitates chloroplast 
            relocation movement in Marchantia polymorpha.
  JOURNAL   Plant J. 2022 Apr 27. doi: 10.1111/tpj.15787.
  PUBMED    35476214
REFERENCE   2  (bases 1 to 10578)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2022 Apr 27. doi: 10.1111/tpj.15787."
COMMENT     Sequence Label: pB4GWnY-MpRTN1
FEATURES             Location/Qualifiers
     source          1..10578
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(23..535,536..538,539..541))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     216..235
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     475..496
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    552..576
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1309..1333)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(1355..1700)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(1392..1412)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     complement(2215..2232)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2215..2231)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2224..2246)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    2434..2458
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3759..4388
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4817..5890
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      5956..6150
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6386..6408)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6494..6634
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6549..6568
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6649..6666)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6820..7408)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6900..6919)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7654..8445)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    8966..8990
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        9164..9343
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        9196..9379
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             9400..10194
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9454..9473)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10064..10083
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 aattcgagct ctaagcctag tcctcgatgt tgtggcggat cttgaagttc accttgatgc
       61 cgttcttctg cttgtcggcc atgatataga cgttgtggct gttgtagttg tactccagct
      121 tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca
      181 ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga
      241 agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct
      301 gcttcatgtg gtcggggtag cgggcgaagc actgcaggcc gtagccgaag gtggtcacga
      361 gggtgggcca gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc
      421 cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc
      481 cgtccagctc gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca
      541 tgctgttatc aaccactttg tacaagaaag ctgggtcctg cgccttcttg tccttgggcg
      601 cagccctagg gattttactc agcaggagag catcgatctt tttgtactgg acattagcct
      661 gatcaactgc aaccttgccg tacttgtcaa cttcatcttc gtacttctca tacagagctg
      721 ggaccgtatg ggaggcgaca acaccgatgt acacaagtgt caagaagttg aaccatccac
      781 caataacaga gagcacccag aggccggcaa ccaccttcag aaagagcttc caatcccttc
      841 cgagagcaac atcgtgtgct attgcaagga ttttgttgat ttcaacgcgg agagtttttg
      901 cagtgtgaac cacggtgtct tcagatagcg caagttcagg tagtggaggt ggagctctgt
      961 tcagaagacc agcagcattc gaccacaaga agaagatgac aatgaggaag agaagcacgt
     1021 tcgagacgag ggacagtaga gtgtaaccag accactcgaa tagaatccaa ccgattgtcg
     1081 ctcccgctag aacaccaccg gacaaaggct tgtgcttcca caaaaggacg tccgcagctt
     1141 tgccaccgcc aagtagctga tgaactgtct tttccctgtt aaatagccgg ccatgtgaag
     1201 atttgcccac ggcatgtgat gatgtcgccg cggggagctt cgacaggttt tccaccacgt
     1261 tgtttacagc ggccgccact gctccttccg ccacatgttc agacatgaag cctgcttttt
     1321 tgtacaaact tgttgataac tctagagtcc cccgtgttct ctccaaatga aatgaacttc
     1381 cttatataga ggaagggtct tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag
     1441 tggagatatc acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca
     1501 cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttcaa
     1561 cgatggcctt tcctttatcg caatgatggc atttgtagga gccaccttcc ttttccacta
     1621 tcttcacaat aaagtgacag atagctgggc aatggaatcc gaggaggttt ccggatatta
     1681 ccctttgttg aaaagtctca attgcccttt ggtcttctga gactgtatct ttgatatttt
     1741 tggagtagac aagtgtgtcg tgctccacca tgttgacgaa gattttcttc ttgtcattga
     1801 gtcgtaagag actctgtatg aactgttcgc cagtctttac ggcgagttct gttaggtcct
     1861 ctatttgaat ctttgactcc atggcctttg attcagtggg aactaccttt ttagagactc
     1921 caatctctat tacttgcctt ggtttgtgaa gcaagccttg aatcgtccat actggaatag
     1981 tacttctgat cttgagaaat atatctttct ctgtgttctt gatgcagtta gtcctgaatc
     2041 ttttgactgc atctttaacc ttcttgggaa ggtatttgat ttcctggaga ttattgctcg
     2101 ggtagatcgt cttgatgaga cctgctgcgt aagcctctct aaccatctgt gggttagcat
     2161 tctttctgaa attgaaaagg ctaatctggg gacctgcagg catgcaagct tggcactggc
     2221 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     2281 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     2341 ccaacagttg cgcagcctga atggcgaatg ctagagcagc ttgagcttgg atcagattgt
     2401 cgtttcccgc cttcagttta aactatcagt gtttgacagg atatattggc gggtaaacct
     2461 aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa aaggtttatc
     2521 cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc aaagtacttt
     2581 gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc agccgtcttc
     2641 tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc tgcccttttc
     2701 ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg actagaaccg
     2761 gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg cccgcgtcag
     2821 caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa
     2881 gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct
     2941 tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc tggcccgcag
     3001 cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg gcctgcgtag
     3061 cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt tgaccgtgtt
     3121 cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga gcgggcgcga
     3181 ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc cggcacagat
     3241 cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg cggctgcact
     3301 gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc
     3361 caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg ccgacgccct
     3421 ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca ggacggccag
     3481 gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg
     3541 ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct
     3601 gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga gcgccgccgt
     3661 ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg
     3721 atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc gctgtactta
     3781 accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc gccctgcaac
     3841 tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg
     3901 cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc
     3961 gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg
     4021 cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg gtgcagccaa
     4081 gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag cgcattgagg
     4141 tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca
     4201 tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt gagtcccgta
     4261 tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag
     4321 aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac
     4381 tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc taagtgccgg
     4441 ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag acacgccagc
     4501 catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga agatgtacgc
     4561 ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc agctaccaga
     4621 gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga ggcggcatgg
     4681 aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga ggaacgggcg
     4741 gttggccagg cgtaagcggc tgggttgtct gccggccctg caatggcact ggaaccccca
     4801 agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc
     4861 gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat
     4921 cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga
     4981 atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca agggcgacga
     5041 gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata gtcgcagcat
     5101 catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg
     5161 ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca tggccagtgt
     5221 gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca tgaaccgata
     5281 ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg cggacgtact
     5341 caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag aaacctgcat
     5401 tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga acggccgcct
     5461 ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa agagcgaaac
     5521 cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg agatcacaga
     5581 aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg atcccggcat
     5641 cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag ccagatggtt
     5701 gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt tctgtttcac
     5761 cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg
     5821 gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc
     5881 cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg
     5941 aaaaggtctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt acattgggaa
     6001 ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac acatgtaagt
     6061 gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa aacttattaa
     6121 aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag ccgaagagct
     6181 gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc
     6241 tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc taccagggcg
     6301 cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca ccctgcctcg
     6361 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     6421 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     6481 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct
     6541 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     6601 gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga
     6661 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     6721 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     6781 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     6841 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     6901 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     6961 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     7021 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     7081 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     7141 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     7201 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     7261 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     7321 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     7381 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     7441 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgcatgata tatctcccaa
     7501 tttgtgtagg gcttattatg cacgcttaaa aataataaaa gcagacttga cctgatagtt
     7561 tggctgtgag caattatgtg cttagtgcat ctaacgcttg agttaagccg cgccgcgaag
     7621 cggcgtcggc ttgaacgaat ttctagctag acattatttg ccgactacct tggtgatctc
     7681 gcctttcacg tagtggacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc
     7741 ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact gggccggcag
     7801 gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct
     7861 gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg
     7921 cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg
     7981 atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt
     8041 cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc
     8101 attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat
     8161 gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga
     8221 agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac
     8281 ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat ccactgcgga
     8341 gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac
     8401 ctctgatagt tgagtcgata cttcggcgat caccgcttcc cccatgatgt ttaactttgt
     8461 tttagggcga ctgccctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc
     8521 cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtaccc caaaaaaaca
     8581 gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt
     8641 tctggaccag ttgcgtgagc gcatacgcta cttgcattac agcttacgaa ccgaacaggc
     8701 ttatgtccac tgggttcgtg cccgaattga tcacaggcag caacgctctg tcatcgttac
     8761 aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg
     8821 ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc
     8881 tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc
     8941 ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc
     9001 ttagacaact taataacaca ttgcggacgt ttttaatgta ctgaattaac gccgaattag
     9061 gctgaattta tttctgaatt catcatgagc ggagaattaa gggagtcacg ttatgacccc
     9121 cgccgatgac gcgggacaag ccgttttacg tttggaactg acagaaccgc aacgttgaag
     9181 gagccactca gccgcgggtt tctggagttt aatgagctaa gcacatacgt cagaaaccat
     9241 tattgcgcgt tcaaaagtcg cctaaggtca ctatcagcta gcaaatattt cttgtcaaaa
     9301 atgctccact gacgttccat aaattcccct cggtatccaa ttagagtctc atattcactc
     9361 tcaatccaaa taatctgcac cggatctgga tcgtttcgca tgattgaaca agatggattg
     9421 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     9481 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     9541 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     9601 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     9661 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     9721 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     9781 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     9841 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     9901 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgatgatct cgtcgtgacc
     9961 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
    10021 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
    10081 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
    10141 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
    10201 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
    10261 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
    10321 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccacgggatc tctgcggaac
    10381 aggcggtcga aggtgccgat atcattacga cagcaacggc cgacaagcac aacgccacga
    10441 tcctgagcga caatatgatc gggcccggcg tccacatcaa cggcgtcggc ggcgactgcc
    10501 caggcaagac cgagatgcac cgcgatatct tgctgcgttc ggatattttc gtggagttcc
    10561 cgccacagac ccggatga
//
