LOCUS       sequence-456250-       13194 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for the expression of MpPHOT fused to the N-terminal half of 
            YFP in plants (for Agrobacterium-mediated genetic transformation.).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13194)
  AUTHORS   Ishikawa K, Konno R, Hirano S, Fujii Y, Fujiwara M, Fukao Y, Kodama 
            Y
  TITLE     The ER membrane-bending protein RETICULON facilitates chloroplast 
            relocation movement in Marchantia polymorpha.
  JOURNAL   Plant J. 2022 Apr 27. doi: 10.1111/tpj.15787.
  PUBMED    35476214
REFERENCE   2  (bases 1 to 13194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2022 Apr 27. doi: 10.1111/tpj.15787."
COMMENT     Sequence Label: pB4GWnY-MpPHOT
FEATURES             Location/Qualifiers
     source          1..13194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(385..1179)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(496..515)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1106..1125
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1200..1383)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(1236..1415)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    1589..1613
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2134..2925
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3171..3759
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3660..3679
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    3945..4085
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4011..4030)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4171..4193
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4429..4623
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(4689..5762)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(6191..6820)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    8121..8145
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     8333..8355
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8347..8364
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8348..8364
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8879..9224
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     9167..9187
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    9246..9270
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(12619..12643)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(12654..12656,12657..12659,12660..13172)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(12699..12720)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(12960..12979)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tcatccgggt ctgtggcggg aactccacga aaatatccga acgcagcaag atatcgcggt
       61 gcatctcggt cttgcctggg cagtcgccgc cgacgccgtt gatgtggacg ccgggcccga
      121 tcatattgtc gctcaggatc gtggcgttgt gcttgtcggc cgttgctgtc gtaatgatat
      181 cggcaccttc gaccgcctgt tccgcagaga tcccgtgggc gaagaactcc agcatgagat
      241 ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt
      301 catagaaggc ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg
      361 tcatttcgaa ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat
      421 gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc
      481 gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac
      541 acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg
      601 caagcaggca tcgccatggg tcacgacgag atcatcgccg tcgggcatgc gcgccttgag
      661 cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc
      721 gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc
      781 gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga
      841 tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa
      901 tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc
      961 cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga
     1021 caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc
     1081 atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc
     1141 ggccggagaa cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc cagatccggt
     1201 gcagattatt tggattgaga gtgaatatga gactctaatt ggataccgag gggaatttat
     1261 ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg accttaggcg
     1321 acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa actccagaaa
     1381 cccgcggctg agtggctcct tcaacgttgc ggttctgtca gttccaaacg taaaacggct
     1441 tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctccgc tcatgatgaa
     1501 ttcagaaata aattcagcct aattcggcgt taattcagta cattaaaaac gtccgcaatg
     1561 tgttattaag ttgtctaagc gtcaatttgt ttacaccaca atatatcctg ccaccagcca
     1621 gccaacagct ccccgaccgg cagctcggca caaaatcacc actcgataca ggcagcccat
     1681 cagtccggga cggcgtcagc gggagagccg ttgtaaggcg gcagactttg ctcatgttac
     1741 cgatgctatt cggaagaacg gcaactaagc tgccgggttt gaaacacgga tgatctcgcg
     1801 gagggtagca tgttgattgt aacgatgaca gagcgttgct gcctgtgatc aattcgggca
     1861 cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc
     1921 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt
     1981 ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag
     2041 caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag
     2101 cagggcagtc gccctaaaac aaagttaaac atcatggggg aagcggtgat cgccgaagta
     2161 tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg
     2221 gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat
     2281 ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac
     2341 cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc
     2401 attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt
     2461 ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt
     2521 gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg
     2581 gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc
     2641 ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg
     2701 ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc
     2761 gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag
     2821 gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt
     2881 gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataatgtct agctagaaat
     2941 tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca agcgttagat gcactaagca
     3001 cataattgct cacagccaaa ctatcaggtc aagtctgctt ttattatttt taagcgtgca
     3061 taataagccc tacacaaatt gggagatata tcatgcatga ccaaaatccc ttaacgtgag
     3121 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     3181 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     3241 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     3301 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     3361 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     3421 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3481 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     3541 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     3601 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     3661 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     3721 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     3781 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     3841 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     3901 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
     3961 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
     4021 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg
     4081 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     4141 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     4201 cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc
     4261 gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc catttttgag
     4321 cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga
     4381 agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta tgcacaggcc
     4441 aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa atcgcctttt
     4501 ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg ctttgggttc
     4561 ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta cgtgctatcc
     4621 acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc tagcatctgc
     4681 tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta gcgcatgact
     4741 aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag gtcatttgac
     4801 ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct gccactgcgt
     4861 tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg
     4921 cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc
     4981 acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag ctcgatctcg
     5041 atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat caaggcttca
     5101 ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg catggcaacg
     5161 tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt tccgccatcg
     5221 gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg gccgggcttg
     5281 tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac cgccatcagt
     5341 accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac ctctacgtgc
     5401 ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg
     5461 aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga
     5521 acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct
     5581 gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg
     5641 gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt caacttctcc
     5701 accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc
     5761 acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag acaacccagc
     5821 cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg gcgtcggtgc
     5881 ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat ctactcattt
     5941 attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag ctcggtaatg
     6001 gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc cggcaactga
     6061 aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc agccttgctg
     6121 ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat tttctcttta
     6181 cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg acctcgcggg
     6241 cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt
     6301 agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg gccagcgcct
     6361 cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt
     6421 gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg
     6481 cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct gccttgatcg
     6541 cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag tcgcgccggc
     6601 cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt
     6661 gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa tcgactaaca
     6721 gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc
     6781 ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct tgcgtatttg
     6841 tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt tttactcaaa
     6901 tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc tggccggcca
     6961 ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca cggttgagac
     7021 gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga tctcttcggt
     7081 aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt
     7141 tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg
     7201 cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc
     7261 gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc
     7321 agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg
     7381 cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg
     7441 aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt ggtggtgtcg
     7501 gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg ctcggcaatg
     7561 tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt cacaacgtcg
     7621 ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct ggtgccggtg
     7681 atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc
     7741 aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc ggcgctcttg
     7801 ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg actaaaacac
     7861 gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg
     7921 tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac tgcactatag
     7981 cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg ttggcatgca
     8041 catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg attattctaa
     8101 taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta
     8161 aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc
     8221 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     8281 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     8341 cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggtccc cagattagcc
     8401 ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac gcagcaggtc
     8461 tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt cccaagaagg
     8521 ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag aaagatatat
     8581 ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt cacaaaccaa
     8641 ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca aaggccatgg
     8701 agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc gaacagttca
     8761 tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg gtggagcacg
     8821 acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa agggcaattg
     8881 agacttttca acaaagggta atatccggaa acctcctcgg attccattgc ccagctatct
     8941 gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc catcattgcg
     9001 ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa gatggacccc
     9061 cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg
     9121 attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag
     9181 acccttcctc tatataagga agttcatttc atttggagag aacacggggg actctagagt
     9241 tatcaacaag tttgtacaaa aaagcaggct tcatgatgcc ctccacggat tcatcgtctg
     9301 cgaagcctta caatctgcgc aagcaagggg acacgcccac aaaacccgac gtgggtcgag
     9361 acgctcgagg ctctctcgaa gtattcggcg gggcgccaca aacctcagca ttgctgttct
     9421 cgaggtcagc tgcggaagat gccttggcag cggccggaat tcgaccctcg caaattctga
     9481 gcccctcggg ctccggcagg cttctgcccg cgctcggccc gttagggtcg gacggacagg
     9541 cgctgccgct gtcgcagtcc atgctcaagg agcgcgagaa gcaagttctg ggcacagagc
     9601 gcggcgtgag aacggactcg ggacgcgcca gggatcaagt ggacaggtgg ctggctactg
     9661 tcgacgagcg cgctccgcct tccaaaggct cggccaagga gccgagcacg gatttctcgt
     9721 tggacgacct ggagctgcca tcttccggcg ctgctgctgg cccaggagcc agaggcgcag
     9781 cagccttggc ctcgcccgaa tgggctgggg ccaaaggcaa agctgtggtc gagaaggagc
     9841 cgagcgaagt ggccggagga ggcttccagg cgcccgaagg tgtgtttccc ctggggggcg
     9901 acaaggtggt gcccatggtg tcgggcccgt cggacttgtc agaggaggtg caggccgagc
     9961 gagccgcgca atggggagtg ctgatgaaga gcacggcaga tctggcctcg accggccgcg
    10021 cgtccgggtc caaggacgag cggatttcgg cctccggcat gtcccgcaga acttcggaag
    10081 gatccgaagg cgaggttcct cgtgtctcta gagatttgaa agatgctctc gcttctttcc
    10141 agcagacctt tgtggtctcg gatgccacac gcacggactt ccccattctg tatgccagtg
    10201 cgggattttt cgccatgaca ggttacactc ccagagaggt catcggaaga aactgtcgat
    10261 ttctccaggg acctgatacc gaccaaagcg acatagagag aattcgaact gctctcaagg
    10321 aggggaagag ttattgtgga agacttctga attacaggaa agacggaact cctttctgga
    10381 accttctgac aattgcacct atcaaggacg acactgggaa agtgctcaaa tacattggaa
    10441 tgcaggtcga ggtcagcaaa tacacagaag gaaacaagtc caatgaagtt cggccgaacg
    10501 gaatgccggc ttcacttatc aaatacgacg ctcggcagca ggacagagcc acgagttctg
    10561 ttcaggaact ggtgggagct ttgaaacatc ctcaccaatc accgcaagat aagccctcgg
    10621 aaggaggcat gcaatcgatg ttttcagttc ctccattgaa agacgaactg cagttgaagg
    10681 taccctcacg cttttcaacg cagtcggttg tcgcactgcc gctcgagggt agccaatcgg
    10741 cgaaagcaag ggcaaaaagt gagtcagtga gtcgactgac cgatgtccca gaacaacctg
    10801 gaatggctga tggcggcctt cgaggggggc agagccaccg cagatcgtcg gggttgttgc
    10861 aaatgctcag gaaaccgaaa tcacaacagc cagaagctga tgtcgtcgac atcgatgact
    10921 tggaccttga cgatgatgcc ccacagacca tggatgagag gccagagagt attgatgaca
    10981 gtgagcgagc caaggaaatt cgtaggggaa tggatttggc cacaacgtta gagcgaattg
    11041 agaagaattt cgtgattaca gatcctcgat tgccggataa ccccattatt ttcgcatccg
    11101 accacttctt agagttaacg gagtatactc gagaagaaat tatcggaaga aattgtcggt
    11161 ttctgcaagg gcccgatacg gacatggatg ttgttcggaa aattagtgat gctatcaaac
    11221 aacagcaaaa catcactgtt caacttctga attacaccaa aggcggaaaa ccgttttgga
    11281 atttgtttca cttggaagca atgaaggaca acaagggtga gttgcaatat tttatcggag
    11341 tccagctcga tggaagtgag cacatcgaac ccatcaggcg acgactctcc gaaaagactg
    11401 aagaggaagg caaaaggatt gtgcaagcta cagcgaagaa tgttgacgga gcagtgaggg
    11461 agcttccaga tgcgaatatg agcatcgagg atttgtgggc gaatcactct cgtgttgtct
    11521 ttcctaggcc tcacaaacgt cagagttcca catggcatgc catgcgaaag atactcagtt
    11581 ctggagacaa attgggtctc aaccacttca ggccaatcaa acctcttggt tgtggagata
    11641 ccggcagtgt acatctggtg gagctgagag ggacgagtga gttctatgcc atgaaagcca
    11701 tggataagaa tgttatggtg aatcggaata aggttcaccg tgctcgggca gagagagaaa
    11761 ttctggagaa aatggatcat ccgtttttgc ccacgttata tggctccttc cagactagga
    11821 cacatgtgtg tctcatcacc gatttttgtc ctggtggtga gctgttcttg cttttggaaa
    11881 gacaacccca gaagaagttc cgtgaggagt ctgctaggtt ttatgcagcc gagattgttc
    11941 ttgccctcga atatcttcac tgcatgggtg ttgtatacag ggacctgaaa cctgaaaacg
    12001 ttctggtcca aaaagatggg catgtgcagc tcactgattt cgacctttcc ttcttgactg
    12061 cctctcgtcc tcagctgctg aaaccagtcg taccatctgg ccgaaagaat agacgagcaa
    12121 gggagaacct acgaccaatc ctccttacag agccaatagc aaagtctaac tccttcgttg
    12181 gaactgagga gtatattgcg ccggaaatta tccttggttt gggacacaac agctctgtag
    12241 attggtgggc tctaggtatt cttctgtacg agatgttatt tggacgaaca ccctttagag
    12301 gacgcaaccg gcaaaggact ttcgcaaatg ttctgcataa agatcttgcc ttcccaagct
    12361 ccatacctgt aagcttggct gccaaacaag ttattcgtgg gctgctacag agagatccca
    12421 agaagaggtt ggggtcagac aagggagccc acgacttaaa acaacatcaa ttcttccggg
    12481 gcatcaattg gcctctcatc cgctgcatga ctcctccacc gctagaaaca ccaatctacc
    12541 aaatcggcaa agaagcggac tccaaagacc tggactggga ggagttggat cccagtgccg
    12601 cctcatttga tgaatatgac ccagctttct tgtacaaagt ggttgataac agcatggtga
    12661 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
    12721 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
    12781 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
    12841 ccaccttcgg ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg
    12901 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
    12961 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
    13021 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
    13081 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
    13141 aggtgaactt caagatccgc cacaacatcg aggactaggc ttagagctcg aatt
//
