LOCUS       sequence-456253-       21667 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR-Cas9 vector including GRF4-GIF1. For wheat and other monocots
            CRISPR experiments. Includes two AarI sites for gRNA cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21667)
  AUTHORS   Debernardi JM, Tricoli DM, Ercoli MF, Hayta S, Ronald P, Palatnik 
            JF, Dubcovsky J
  TITLE     A GRF-GIF chimeric protein improves the regeneration efficiency of 
            transgenic plants.
  JOURNAL   Nat Biotechnol. 2020 Oct 12. pii: 10.1038/s41587-020-0703-0. doi: 
            10.1038/s41587-020-0703-0.
  PUBMED    33046875
REFERENCE   2  (bases 1 to 21667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Oct 12. pii: 10.1038/s41587-020-0703-0. doi: 
            10.1038/s41587-020-0703-0."
COMMENT     Sequence Label: JD633
FEATURES             Location/Qualifiers
     source          1..21667
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1233..1862
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2291..3364
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3430..3624
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3860..3882)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3968..4108
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4023..4042
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4123..4140)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4294..4882)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4374..4393)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4969..5763)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    6188..6212
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    6290..6464
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(6505..7530)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     protein_bind    9470..9491
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9506..9511,9512..9529,9530..9536)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9544..9560
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9568..9584
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11629..11653
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      11650..11659
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(13568..13592)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             13621..13710
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      13744..13996
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             16022..20125
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             16022..20125
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             20126..20173
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      20184..20432
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     CDS             complement(20810..21115)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGTCL"
     primer_bind     20980..20996
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     21006..21025
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        21006..21024
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(21072..21092)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(21072..21090)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(21111..21127)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    21135..21151
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(21159..21165,21166..21183,21184..21189))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    21204..21225
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_RNA        21264..21339
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(21356..21372)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    21575..21599
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca
       61 acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa
      121 cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg
      181 ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca
      241 ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga
      301 cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt
      361 ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca
      421 cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg
      481 cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc
      541 agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg
      601 cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc
      661 caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca
      721 cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg
      781 cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga
      841 ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc
      901 cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa
      961 ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag
     1021 ccgcccgcgc acggctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc
     1081 aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa
     1141 aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga
     1201 tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga
     1261 aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg
     1321 gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg
     1381 tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg
     1441 tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact
     1501 tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt
     1561 acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg
     1621 atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg
     1681 gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc
     1741 agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg
     1801 agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt
     1861 gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc
     1921 gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa
     1981 gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg
     2041 ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat
     2101 gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc
     2161 aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc
     2221 caggcgtaag cggctgggtt gcctgccggc cctgcaatgg cactggaacc cccaagcccg
     2281 aggaatcggc gtgagcggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg
     2341 tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc
     2401 agaagcacgc cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg
     2461 gcaaccgccg gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc
     2521 agattttttc gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga
     2581 cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga
     2641 gcttccagac gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga
     2701 ttacgacctg gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga
     2761 agggaaggga gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt
     2821 ctgccggcga gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt
     2881 aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac
     2941 ggtatccgag ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg
     3001 gccggagtac atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa
     3061 gaacccggac gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg
     3121 ttttctctac cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa
     3181 gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg
     3241 caagctgatc gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc
     3301 tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc
     3361 ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg
     3421 cctctttcct gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa
     3481 cccgtacatt gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga
     3541 tataaaagag aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct
     3601 taaaacccgc ctggcctgtg cataactgtc tggccagcgc acagcccaag agctgcaaaa
     3661 agcgcctacc cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc
     3721 ggccgctggc cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca
     3781 agccgcgccg tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt
     3841 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggaaacggtc acagcttgtc
     3901 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     3961 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
     4021 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     4081 atgcgtaagg agaaaatacc gcatcaggcc ctcttccgct tcctcgctca ctgactcgct
     4141 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     4201 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     4261 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     4321 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     4381 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     4441 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     4501 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     4561 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     4621 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     4681 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     4741 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     4801 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     4861 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     4921 gtggaacgaa aactcacgtt aagggatttt ggtcatgcat tctaggtact aaaacaattc
     4981 atccagtaaa atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa
     5041 aaatagctcg acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc
     5101 aatgtcatac cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt
     5161 gccatctttc acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc
     5221 ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc
     5281 ttcttcccag ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc
     5341 ggctaagcgg ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa
     5401 gagcctgatg cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata
     5461 ctcttccgag caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg
     5521 ccgttcaaag tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc
     5581 cttttcccgt tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata
     5641 taggttttca ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc
     5701 ttttacgcag cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc
     5761 catttattat ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat
     5821 aacaagacga actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca
     5881 gctttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg
     5941 aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc
     6001 cgcgagatca tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg
     6061 gtaacatgag caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg
     6121 atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc
     6181 tggctggtgg caggatatat tgtggtgtaa acaaattgac gcttagacaa cttaataaca
     6241 cattgcggac gtttttaatg tactgaatta acgccgaatt aattcggggg atctggattt
     6301 tagtactgga ttttggtttt aggaattaga aattttattg atagaagtat tttacaaata
     6361 caaatacata ctaagggttt cttatatgct caacacatga gcgaaaccct ataggaaccc
     6421 taattccctt atctgggaac tactcacaca ttattatgga gaaactcgag cttgtcgatc
     6481 gacagatccc ggtcggcatc tactctattt ctttgccctc ggacgagtgc tggggcgtcg
     6541 gtttccacta tcggcgagta cttctacaca gccatcggtc cagacggccg cgcttctgcg
     6601 ggcgatttgt gtacgcccga cagtcccggc tccggatcgg acgattgcgt cgcatcgacc
     6661 ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag ctctgataga gttggtcaag
     6721 accaatgcgg agcatatacg cccggagtcg tggcgatcct gcaagctccg gatgcctccg
     6781 ctcgaagtag cgcgtctgct gctccataca agccaaccac ggcctccaga agaagatgtt
     6841 ggcgacctcg tattgggaat ccccgaacat cgcctcgctc cagtcaatga ccgctgttat
     6901 gcggccattg tccgtcagga cattgttgga gccgaaatcc gcgtgcacga ggtgccggac
     6961 ttcggggcag tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga cggacgcact
     7021 gacggtgtcg tccatcacag tttgccagtg atacacatgg ggatcagcaa tcgcgcatat
     7081 gaaatcacgc catgtagtgt attgaccgat tccttgcggt ccgaatgggc cgaacccgct
     7141 cgtctggcta agatcggccg cagcgatcgc atccatagcc tccgcgaccg gttgtagaac
     7201 agcgggcagt tcggtttcag gcaggtcttg caacgtgaca ccctgtgaac ggcgggagat
     7261 gcaataggtc aggctctcgc taaactcccc aatgtcaagc acttccggaa tcgggagcgc
     7321 ggccgatgca aagtgccgat aaacataacg atctttgtag aaaccatcgg cgcagctatt
     7381 tacccgcagg acatatccac gccctcctac atcgaagctg aaagcacgag attcttcgcc
     7441 ctccgagagc tgcatcaggt cggacacgct gtcgaacttt tcgatcagaa acttctcgac
     7501 agacgtcgcg gtgagttcag gctttttcat aagcttctgc aaaagagaac cagacaacag
     7561 ggtaagtgcc tagcagtaaa caaacagaac tcatcacaag caaacagcaa catcatattc
     7621 ataccaacag gtcatgtgtg ttcatcacat cattagtact aagcatgcca tcatccaagt
     7681 atatcaaagt aagggcaaag agcatgcatg atcatcaggt gcacaaaaga atcatcaaat
     7741 tgtagcagta catatcttca tctatcatgc atatctatcc ataacaggac gatgcatgtt
     7801 gaccaggtaa aagctacagg atcctatagg aacagcagcg tatatatctt caccaatctt
     7861 agcatcttaa tcatgtggca catgcagttt caatttaagc acatgagcta gttgattatg
     7921 aggtaccaga gaatcatcaa actgatgtag cagcatatat cctcatctat catgcaatct
     7981 aatctaatct aactaaacag gaaaggtgtg ctattcagtt aaaagctacc gcatcatata
     8041 caaacggcag cataagaaaa agcataatca tcttaatcat gcaacaaacg cagattcata
     8101 ataagcccaa gagctagctt gtgatgatct tattctactc tgatctacag caatcagata
     8161 acgacctaac cttgcacatg gcaacaaaac aatcgatcgg acgaatcagt tgtttgttcc
     8221 tagctagcac catcgaacca gataatagat gcacgtacag atcccgaaaa cgaacccaaa
     8281 aacagggcag acctagctga acctaggcag cgacccagca gatcgtgaga acgatctcat
     8341 ctacgaacag cctagaagca accccacgat tcccggacaa acgacctaaa atccccacaa
     8401 atcacatgag catgacagga taaacagcgg aaccgatcag atctacacga aaaccccacc
     8461 tcccagccac ccacgatcag gaaacacgcg gatctagcat gatttcgtca acgcctcagc
     8521 ctagttccta gccacagacc aagcagaacc accaaaccac gccgagcgag gagatggggc
     8581 aagaggacgg gggagacgat cgccgtacct tgaagcgggg gaaggatcgc cgagggtcgc
     8641 gaggagagca atttggattt ggaacccggg ggttgtgcgc tccgaacgat gagacgatgt
     8701 gagattgtgg gaagaggcgc ggagggccct gtatttatgg gctgcgacgg ggggaggaga
     8761 ggtggggagg gttggggaag gaatccccca cccgtgccgt gacggttccg ggccgtgtga
     8821 gaggagcccg ctcgtctccg ccacgcaatt tccgcgatcg gagcggagct ttcgagaggc
     8881 ggctggatgg ttggtggccg ttagatttgt agacgccgtt aacgcctcgc ctccaccggg
     8941 aagagttttg agcagccgct tatgacaatg gcttaacgac gttagacgga gcgttagtgg
     9001 caggccatga tagaatagac gtattgcaat gggattataa ttaaataaat aagaatataa
     9061 taagatatgg caagtcggca ctcatgacat ggtcttcgaa atgatagtgc tcactttctt
     9121 agccgagaaa gttgacgcga ctgatttaga agttaagata ttatttctct cttcttttct
     9181 ttcctcgtca tataaggatg aaataaactt tagagattgc cggtagtgat tttggatttc
     9241 ggcgatcagg cttggtttgc cggtttcgga cggtgtgcct taggccaccc gcagtgtatc
     9301 ttgtaatgtt caaccgataa gcaagggtgg ggctcaagca agtagtaaac aactatgtca
     9361 aatgtcacca tggttatggt cttgtttagt tggcttcttg gccgattcat taatgcagct
     9421 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     9481 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     9541 gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt acgaattcga
     9601 gctcggtacc cggggatctg caggcgcgtg cagcgtgacc cggtcgtgcc cctctctaga
     9661 gataatgagc attgcatgtc taagttataa aaaattacca catatttttt ttgtcacact
     9721 tgtttgaagt gcagtttatc tatctttata catatattta aactttactc tacgaataat
     9781 ataatctata gtactacaat aatatcagtg ttttagagaa tcatataaat gaacagttag
     9841 acatggtcta aaggacaatt gagtattttg acaacaggac tctacagttt tatcttttta
     9901 gtgtgcatgt gttctccttt ttttttgcaa atagcttcac ctatataata cttcatccat
     9961 tttattagta catccattta gggtttaggg ttaatggttt ttatagacta atttttttag
    10021 tacatctatt ttattctatt ttagcctcta aattaagaaa actaaaactc tattttagtt
    10081 tttttattta ataatttaga tataaaatag aataaaataa agtgactaaa aattaaacaa
    10141 atacccttta agaaattaaa aaaactaagg aaacattttt cttgtttcga gtagataatg
    10201 ccagcctgtt aaacgccgtc gacgagtcta acggacacca accagcgaac cagcagcgtc
    10261 gcgtcgggcc aagcgaagca gacggcacgg catctctgtc gctgcctctg gacccctctc
    10321 gagagttccg ctccaccgtt ggacttgctc cgctgtcggc atccagaaat tgcgtggcgg
    10381 agcggcagac gtgagccggc acggcaggcg gcctcctcct cctctcacgg caccggcagc
    10441 tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc cgtaataaat
    10501 agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc acacacacac
    10561 aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg ccgctcgtcc
    10621 tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg ttagggcccg
    10681 gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat ccgtgctgct
    10741 agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta acttgccagt
    10801 gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga tcgatttcat
    10861 gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt tcaatatatg
    10921 ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt tgtgatgatg
    10981 tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac tacctggtgg
    11041 atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac gaattgaaga
    11101 tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt ttactgatgc
    11161 atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt gggcggtcgt
    11221 tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat ttattaattt
    11281 tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg gatggaaata
    11341 tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata catgatggca
    11401 tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa taaacaagta
    11461 tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag cagctatatg
    11521 tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt ttcttttgtc
    11581 gatgctcacc ctgttgtttg gtgttacttc tgcagactag agttatcaac aagtttgtac
    11641 aaaaaagctg ccaccatggc gatgccgtat gcctctcttt ccccggcagg cgaccgccgc
    11701 tcctccccgg ccgccaccgc caccgcctcc ctcctcccct tctgccgctc ctcccccttc
    11761 tccgccggcg gcaatggcgg catgggggag gaggcgcgga tggacgggag gtggatggcg
    11821 aggccggtgc ccttcacggc ggcgcagtac gaggagctgg agcaccaggc gctcatatac
    11881 aagtacctgg tggccggcgt gtccgtcccg ccggatctcg tgctccccat ccgccgcggc
    11941 atcgagtccc tcgccgcccg cttctaccac aaccccctcg ccatcgggta cggatcgtac
    12001 ctgggcaaga aggtggatcc ggagccgggc cggtgccggc gcacggacgg caagaagtgg
    12061 cggtgcgcca aggaggccgc ctccgactcc aagtattgcg agcgccacat gcaccgcggc
    12121 cgcaaccgtt caagaaagcc tgtggaaacg cagctcgtct cgcactccca gccgccggcc
    12181 gcctccgtcg tgccgcccct cgccaccggc ttccacaacc actccctcta ccccgccatc
    12241 ggcggcacca acggtggtgg aggcgggggg aacaacggca tgtccatgcc cggcacgttc
    12301 tcctccgcgc tggggccgcc tcagcagcac atgggcaaca atgccgcctc tccctacgcg
    12361 gctctcggcg gcgccggaac atgcaaagat ttcaggtata ccgcatatgg aataagatct
    12421 ttggcagacg agcagagtca gctcatgaca gaagccatga acacctccgt ggagaaccca
    12481 tggcgcctgc cgccatcttc tcaaacgact acattcccgc tctcaagcta ctctcctcag
    12541 cttggagcaa cgagtgacct gggtcagaac aacagcagca acaacaacag cggcgtcaag
    12601 gccgagggac agcagcagca gcagccgctc tccttcccgg ggtgcggcga cttcggcagc
    12661 ggcgactccg cgaagcagga gaaccagacg ctgcggccgt tcttcgacga gtggccgaag
    12721 acgagggact cgtggtcgga cctgaccgac gacaactcga acgtcgcctc cttctcggcc
    12781 acccagctgt cgatctcgat acccatgacg tcctccgact tctccgccgc cagctcccag
    12841 tcgcccaacg gcatgctgtt cgccggcgaa atgtacgcgg ccgctgccat gcagcagcaa
    12901 cacctgatgc agatgaacca gagcatgatg gggggctacg cttcctctac cactgccacc
    12961 actgatctca ttcagcagta cctggatgag aacaagcagc tgatcctggc catcctcgac
    13021 aaccagaaca acggcaaggt ggaggagtgc gcacggaacc aagctaagct ccagcagaac
    13081 ctcatgtacc tcgccgccat cgccgacagc cagcctccgc agacggcatc gctgtctcag
    13141 tacccgtcca acctgatgat gcagtccggg ccgcggtaca tgcagcagca gtcggcgcag
    13201 atgatgtcgc cgcagtcgct gatggcggcg cggtcgtcga tgatgtacgc gcagcaggcc
    13261 atgtcgccgc tccagcagca gcagcagcag cagcagcacc aggcggccgc gcacggccag
    13321 ctggggatgt cctccggcgc gaccaccggg ttcaacctcc tgcacggtga ggccagcatg
    13381 ggcggcggcg gcggcgccac tggcaacagc atgatgaacg ccagcgtctt ctcggactat
    13441 ggccgcggcg gcagcggcgc caaggagggg tcgacctcgc tgtcggccga cgctcgcggc
    13501 gccaactctg gcgcgcacag cggcgacggg gagtacctca agggcaccga ggaggaagga
    13561 agctagcgtt cagctttctt gtacaaagtg gttgataaca gcgggttaat taacatcttt
    13621 tacccatacg atgttcctga ctatgcgggc tatccctatg acgtcccgga ctatgcagga
    13681 tcctatccat atgacgttcc agattacgct gctcagtgca gcgcttagag ctcgaatttc
    13741 cccgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt
    13801 gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa
    13861 tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa
    13921 tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca
    13981 tctatgttac tagatcgggg attagcgcgc ctgcagtgca gcgtgacccg gtcgtgcccc
    14041 tctctagaga taatgagcat tgcatgtcta agttataaaa aattaccaca tatttttttt
    14101 gtcacacttg tttgaagtgc agtttatcta tctttataca tatatttaaa ctttactcta
    14161 cgaataatat aatctatagt actacaataa tatcagtgtt ttagagaatc atataaatga
    14221 acagttagac atggtctaaa ggacaattga gtattttgac aacaggactc tacagtttta
    14281 tctttttagt gtgcatgtgt tctccttttt ttttgcaaat agcttcacct atataatact
    14341 tcatccattt tattagtaca tccatttagg gtttagggtt aatggttttt atagactaat
    14401 ttttttagta catctatttt attctatttt agcctctaaa ttaagaaaac taaaactcta
    14461 ttttagtttt tttatttaat aatttagata taaaatagaa taaaataaag tgactaaaaa
    14521 ttaaacaaat accctttaag aaattaaaaa aactaaggaa acatttttct tgtttcgagt
    14581 agataatgcc agcctgttaa acgccgtcga cgagtctaac ggacaccaac cagcgaacca
    14641 gcagcgtcgc gtcgggccaa gcgaagcaga cggcacggca tctctgtcgc tgcctctgga
    14701 cccctctcga gagttccgct ccaccgttgg acttgctccg ctgtcggcat ccagaaattg
    14761 cgtggcggag cggcagacgt gagccggcac ggcaggcggc ctcctcctcc tctcacggca
    14821 ccggcagcta cgggggattc ctttcccacc gctccttcgc tttcccttcc tcgcccgccg
    14881 taataaatag acaccccctc cacaccctct ttccccaacc tcgtgttgtt cggagcgcac
    14941 acacacacaa ccagatctcc cccaaatcca cccgtcggca cctccgcttc aaggtacgcc
    15001 gctcgtcctc cccccccccc ctctctacct tctctagatc ggcgttccgg tccatggtta
    15061 gggcccggta gttctacttc tgttcatgtt tgtgttagat ccgtgtttgt gttagatccg
    15121 tgctgctagc gttcgtacac ggatgcgacc tgtacgtcag acacgttctg attgctaact
    15181 tgccagtgtt tctctttggg gaatcctggg atggctctag ccgttccgca gacgggatcg
    15241 atttcatgat tttttttgtt tcgttgcata gggtttggtt tgcccttttc ctttatttca
    15301 atatatgccg tgcacttgtt tgtcgggtca tcttttcatg cttttttttg tcttggttgt
    15361 gatgatgtgg tctggttggg cggtcgttct agatcggagt agaattaatt ctgtttcaaa
    15421 ctacctggtg gatttattaa ttttggatct gtatgtgtgt gccatacata ttcatagtta
    15481 cgaattgaag atgatggatg gaaatatcga tctaggatag gtatacatgt tgatgcgggt
    15541 tttactgatg catatacaga gatgcttttt gttcgcttgg ttgtgatgat gtggtgtggt
    15601 tgggcggtcg ttcattcgtt ctagatcgga gtagaatact gtttcaaact acctggtgta
    15661 tttattaatt ttggaactgt atgtgtgtgt catacatctt catagttacg agtttaagat
    15721 ggatggaaat atcgatctag gataggtata catgttgatg tgggttttac tgatgcatat
    15781 acatgatggc atatgcagca tctattcata tgctctaacc ttgagtacct atctattata
    15841 ataaacaagt atgttttata attattttga tcttgatata cttggatgat ggcatatgca
    15901 gcagctatat gtggattttt ttagccctgc cttcatacgc tatttatttg cttggtactg
    15961 tttcttttgt cgatgctcac cctgttgttt ggtgttactt cctgcagggc gatctattcg
    16021 aatggacaag aagtactcga tcggcctcga catcgggacg aactcagttg gctgggccgt
    16081 gatcaccgac gagtacaagg tgccctctaa gaagttcaag gtcctgggga acaccgaccg
    16141 ccattccatc aagaagaacc tcatcggcgc tctcctgttc gacagcgggg agaccgctga
    16201 ggctacgagg ctcaagagaa ccgctaggcg ccggtacacg agaaggaaga acaggatctg
    16261 ctacctccaa gagattttct ccaacgagat ggccaaggtt gacgattcat tcttccaccg
    16321 cctggaggag tctttcctcg tggaggagga taagaagcac gagcggcatc ccatcttcgg
    16381 caacatcgtg gacgaggttg cctaccacga gaagtaccct acgatctacc atctgcggaa
    16441 gaagctcgtg gactccaccg ataaggcgga cctcagactg atctacctcg ctctggccca
    16501 catgatcaag ttccgcggcc atttcctgat cgagggggat ctcaacccag acaacagcga
    16561 tgttgacaag ctgttcatcc aactcgtgca gacctacaac caactcttcg aggagaaccc
    16621 gatcaacgcc tctggcgtgg acgcgaaggc tatcctgtcc gcgaggctct cgaagtccag
    16681 gaggctggag aacctgatcg ctcagctccc aggcgagaag aagaacggcc tgttcgggaa
    16741 cctcatcgct ctcagcctgg ggctcacccc gaacttcaag tcgaacttcg atctcgctga
    16801 ggacgccaag ctgcaactct ccaaggacac ctacgacgat gacctcgata acctcctggc
    16861 ccagatcggc gatcaatacg cggacctgtt cctcgctgcc aagaacctgt cggacgccat
    16921 cctcctgtca gatatcctcc gcgtgaacac cgagatcacg aaggctccac tctctgcctc
    16981 catgatcaag cgctacgacg agcaccatca ggatctgacc ctcctgaagg cgctggtccg
    17041 ccaacagctc ccggagaagt acaaggagat tttcttcgat cagtcgaaga acggctacgc
    17101 tgggtacatc gacggcgggg cctcacaaga ggagttctac aagttcatca agccaatcct
    17161 ggagaagatg gacggcacgg aggagctcct ggtgaagctc aacagggagg acctcctgcg
    17221 gaagcagaga accttcgata acggcagcat cccccaccaa atccatctcg gggagctgca
    17281 cgccatcctg agaaggcaag aggacttcta ccctttcctc aaggataacc gggagaagat
    17341 cgagaagatc ctgaccttca gaatcccata ctacgtcggc cctctcgcgc gggggaactc
    17401 aagattcgct tggatgaccc gcaagtctga ggagaccatc acgccgtgga acttcgagga
    17461 ggtggtggac aagggcgcta gcgctcagtc gttcatcgag aggatgacca acttcgacaa
    17521 gaacctgccc aacgagaagg tgctccctaa gcactcgctc ctgtacgagt acttcaccgt
    17581 ctacaacgag ctcacgaagg tgaagtacgt caccgagggc atgcgcaagc cagcgttcct
    17641 gtccggggag cagaagaagg ctatcgtgga cctcctgttc aagaccaacc ggaaggtcac
    17701 ggttaagcaa ctcaaggagg actacttcaa gaagatcgag tgcttcgatt cggtcgagat
    17761 cagcggcgtt gaggaccgct tcaacgccag cctcgggacc taccacgatc tcctgaagat
    17821 catcaaggat aaggacttcc tggacaacga ggagaacgag gatatcctgg aggacatcgt
    17881 gctgaccctc acgctgttcg aggacaggga gatgatcgag gagcgcctga agacgtacgc
    17941 ccatctcttc gatgacaagg tcatgaagca actcaagcgc cggagataca ccggctgggg
    18001 gaggctgtcc cgcaagctca tcaacggcat ccgggacaag cagtccggga agaccatcct
    18061 cgacttcctg aagagcgatg gcttcgccaa caggaacttc atgcaactga tccacgatga
    18121 cagcctcacc ttcaaggagg atatccaaaa ggctcaagtg agcggccagg gggactcgct
    18181 gcacgagcat atcgcgaacc tcgctggctc ccccgcgatc aagaagggca tcctccagac
    18241 cgtgaaggtt gtggacgagc tcgtgaaggt catgggccgg cacaagcctg agaacatcgt
    18301 catcgagatg gccagagaga accaaaccac gcagaagggg caaaagaact ctagggagcg
    18361 catgaagcgc atcgaggagg gcatcaagga gctggggtcc caaatcctca aggagcaccc
    18421 agtggagaac acccaactgc agaacgagaa gctctacctg tactacctcc agaacggcag
    18481 ggatatgtac gtggaccaag agctggatat caaccgcctc agcgattacg acgtcgatca
    18541 tatcgttccc cagtctttcc tgaaggatga ctccatcgac aacaaggtcc tcaccaggtc
    18601 ggacaagaac cgcggcaagt cagataacgt tccatctgag gaggtcgtta agaagatgaa
    18661 gaactactgg aggcagctcc tgaacgccaa gctgatcacg caaaggaagt tcgacaacct
    18721 caccaaggct gagagaggcg ggctctcaga gctggacaag gccggcttca tcaagcggca
    18781 gctggtcgag accagacaaa tcacgaagca cgttgcgcaa atcctcgact ctcggatgaa
    18841 cacgaagtac gatgagaacg acaagctgat cagggaggtt aaggtgatca ccctgaagtc
    18901 taagctcgtc tccgacttca ggaaggattt ccagttctac aaggttcgcg agatcaacaa
    18961 ctaccaccat gcccatgacg cttacctcaa cgctgtggtc ggcaccgctc tgatcaagaa
    19021 gtacccaaag ctggagtccg agttcgtgta cggggactac aaggtttacg atgtgcgcaa
    19081 gatgatcgcc aagtcggagc aagagatcgg caaggctacc gccaagtact tcttctactc
    19141 aaacatcatg aacttcttca agaccgagat cacgctggcc aacggcgaga tccggaagag
    19201 accgctcatc gagaccaacg gcgagacggg ggagatcgtg tgggacaagg gcagggattt
    19261 cgcgaccgtc cgcaaggttc tctccatgcc ccaggtgaac atcgtcaaga agaccgaggt
    19321 ccaaacgggc gggttctcaa aggagtctat cctgcctaag cggaacagcg acaagctcat
    19381 cgccagaaag aaggactggg acccaaagaa gtacggcggg ttcgacagcc ctaccgtggc
    19441 ctactcggtc ctggttgtgg cgaaggttga gaagggcaag tccaagaagc tcaagagcgt
    19501 gaaggagctc ctggggatca ccatcatgga gaggtccagc ttcgagaaga acccaatcga
    19561 cttcctggag gccaagggct acaaggaggt gaagaaggac ctgatcatca agctcccgaa
    19621 gtactctctc ttcgagctgg agaacggcag gaagagaatg ctggcttccg ctggcgagct
    19681 ccagaagggg aacgagctcg cgctgccaag caagtacgtg aacttcctct acctggcttc
    19741 ccactacgag aagctcaagg gcagcccgga ggacaacgag caaaagcagc tgttcgtcga
    19801 gcagcacaag cattacctcg acgagatcat cgagcaaatc tccgagttca gcaagcgcgt
    19861 gatcctcgcc gacgcgaacc tggataaggt cctctccgcc tacaacaagc accgggacaa
    19921 gcccatcaga gagcaagcgg agaacatcat ccatctcttc accctgacga acctcggcgc
    19981 tcctgctgct ttcaagtact tcgacaccac gatcgatcgg aagagataca cctccacgaa
    20041 ggaggtcctg gacgcgaccc tcatccacca gtcgatcacc ggcctgtacg agacgaggat
    20101 cgacctctca caactcggcg gggataagag acccgcagca accaagaagg cagggcaagc
    20161 aaagaagaag aagtgactcg agatatgaag atgaagatga aatatttggt gtgtcaaata
    20221 aaaagcttgt gtgcttaagt ttgtgttttt ttcttggctt gttgtgttat gaatttgtgg
    20281 ctttttctaa tattaaatga atgtaagatc acattataat gaataaacaa atgtttctat
    20341 aatccattgt gaatgttttg ttggatctct tctgcagcat ataactactg tatgtgctat
    20401 ggtatggact atggaatatg attaaagata aggagctccg gtgacggacg accaagcccg
    20461 ttattctgac agttctggtg ctcaacacat ttatatttat caaggagcac attgttactc
    20521 actgctagga gggaatcgaa ctaggaatat tgatcagagg aactacgaga gagctgaaga
    20581 taactgccct ctagctctca ctgatctggg tcgcatagtg agatgcagcc cacgtgagtt
    20641 cagcaacggt ctagcgctgg gcttttaggc ccgcatgatc gggcttttgt cgggtggtcg
    20701 acgtgttcac gattggggag agcaacgcag cagttcctct tagtttagtc ccacctcgcc
    20761 tgtccagcag agttctgacc ggtttataaa ctcgcttgct gcatcagact tagaggcagg
    20821 tgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat
    20881 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
    20941 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc
    21001 gcgcgtaata cgactcacta tagggcgaat tgggtaccgg gccccccctc gaggtcctcc
    21061 agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg
    21121 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
    21181 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
    21241 ctgcccgctt tccacctgcg gtcgttttag agctagaaat agcaagttaa aataaggcta
    21301 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttcccg gtggcactgg
    21361 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
    21421 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
    21481 cccaacagtt gcgcagcctg aatggcgaat gctagagcag cttgagcttg gatcagattg
    21541 tcgtttcccg ccttcagttt aaactatcag tgtttgacag gatatattgg cgggtaaacc
    21601 taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat
    21661 ccgttcg
//
