LOCUS       sequence-456255-       12477 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector for the expression of MpRTN1 in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12477)
  AUTHORS   Ishikawa K, Konno R, Hirano S, Fujii Y, Fujiwara M, Fukao Y, Kodama 
            Y
  TITLE     The ER membrane-bending protein RETICULON facilitates chloroplast 
            relocation movement in Marchantia polymorpha.
  JOURNAL   Plant J. 2022 Apr 27. doi: 10.1111/tpj.15787.
  PUBMED    35476214
REFERENCE   2  (bases 1 to 12477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2022 Apr 27. doi: 10.1111/tpj.15787."
COMMENT     Sequence Label: pMpGWB306-MpRTN1
FEATURES             Location/Qualifiers
     source          1..12477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    375..399
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             920..1711
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1957..2545
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2446..2465
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2699..2716
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2731..2871
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2797..2816)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2957..2979
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3215..3409
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(3475..4548)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(4977..5606)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    6907..6931
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     7119..7141
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7133..7150
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7134..7150
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7665..8010
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    8032..8056
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8789..8813)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(8824..8826,8827..8829,8830..9540)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8869..8890)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9130..9149)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9477..9498
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      9565..9817
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(9850..9866)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9850..9866)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9863..9885)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9874..9890
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9898..9904,9905..9922,9923..9928))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9943..9964
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    10049..10223
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(join(12312..12477,1..180))
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
ORIGIN
        1 ggtccatctt tgggaccact gtcggcagag gcatcttgaa cgatagcctt tcctttatcg
       61 caatkatggc atttgtaggt gccaccttcc ttttctactg tccttttgat gaagtgacag
      121 atagctgggc aatggaatcc gaggaggttt cccgatatta ccctttgttg aaaagtctca
      181 atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac gagagtgtcg
      241 tgctccacca tgttggcaag ctgctctagc caatacgcaa accgaattca gaaataaatt
      301 cagcctaatt cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt
      361 ctaagcgtca atttgtttac accacaatat atcctgccac cagccagcca acagctcccc
      421 gaccggcagc tcggcacaaa atcaccactc gatacaggca gcccatcagt ccgggacggc
      481 gtcagcggga gagccgttgt aaggcggcag actttgctca tgttaccgat gctattcgga
      541 agaacggcaa ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt
      601 gattgtaacg atgacagagc gttgctgcct gtgatcaatt cgggcacgaa cccagtggac
      661 ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc
      721 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
      781 tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
      841 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
      901 taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga ctcaactatc
      961 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
     1021 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt
     1081 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
     1141 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
     1201 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
     1261 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
     1321 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
     1381 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
     1441 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
     1501 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
     1561 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
     1621 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa
     1681 aggcgagatc accaaggtag tcggcaaata atgtctagct agaaattcgt tcaagccgac
     1741 gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac taagcacata attgctcaca
     1801 gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat aagccctaca
     1861 caaattggga gatatatcat gcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     1921 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1981 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     2041 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     2101 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     2161 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     2221 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     2281 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     2341 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     2401 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     2461 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     2521 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     2581 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     2641 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     2701 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
     2761 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
     2821 gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac
     2881 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     2941 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     3001 cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg
     3061 tccgcgccct ggtagattgc ctggccgtag gccagccatt tttgagcggc cagcggccgc
     3121 gataggccga cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt
     3181 ttgcagctct tcggctgtgc gctggccaga cagttatgca caggccaggc gggttttaag
     3241 agttttaata agttttaaag agttttaggc ggaaaaatcg ccttttttct cttttatatc
     3301 agtcacttac atgtgtgacc ggttcccaat gtacggcttt gggttcccaa tgtacgggtt
     3361 ccggttccca atgtacggct ttgggttccc aatgtacgtg ctatccacag gaaagagacc
     3421 ttttcgacct ttttcccctg ctagggcaat ttgccctagc atctgctccg tacattagga
     3481 accggcggat gcttcgccct cgatcaggtt gcggtagcgc atgactagga tcgggccagc
     3541 ctgccccgcc tcctccttca aatcgtactc cggcaggtca tttgacccga tcagcttgcg
     3601 cacggtgaaa cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt
     3661 gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg
     3721 gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt
     3781 gccttctgtg atctcgcggt acatccaatc agctagctcg atctcgatgt actccggccg
     3841 cccggtttcg ctctttacga tcttgtagcg gctaatcaag gcttcaccct cggataccgt
     3901 caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa
     3961 ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa
     4021 cttgagtacg tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc ccttcccttc
     4081 ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc
     4141 ccacacactg gccatgccgg ccggccctgc ggaaacctct acgtgcccgt ctggaagctc
     4201 gtagcggatc acctcgccag ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc
     4261 catgatgctg cgactatcgc gggtgcccac gtcatagagc atcggaacga aaaaatctgg
     4321 ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca ccggctgccg gcggttgccg
     4381 ggattctttg cggattcgat cagcggccgc ttgccacgat tcaccggggc gtgcttctgc
     4441 ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac ttctccacca ggtcatcacc
     4501 cagcgccgcg ccgatttgta ccgggccgga tggtttgcga ccgctcacgc cgattcctcg
     4561 ggcttggggg ttccagtgcc attgcagggc cggcagacaa cccagccgct tacgcctggc
     4621 caaccgcccg ttcctccaca catggggcat tccacggcgt cggtgcctgg ttgttcttga
     4681 ttttccatgc cgcctccttt agccgctaaa attcatctac tcatttattc atttgctcat
     4741 ttactctggt agctgcgcga tgtattcaga tagcagctcg gtaatggtct tgccttggcg
     4801 taccgcgtac atcttcagct tggtgtgatc ctccgccggc aactgaaagt tgacccgctt
     4861 catggctggc gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg
     4921 acggccggca cttagcgtgt ttgtgctttt gctcattttc tctttacctc attaactcaa
     4981 atgagttttg atttaatttc agcggccagc gcctggacct cgcgggcagc gtcgccctcg
     5041 ggttctgatt caagaacggt tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc
     5101 gtgatacggg actcaagaat gggcagctcg tacccggcca gcgcctcggc aacctcaccg
     5161 ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc
     5221 gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg cggtggccca tatgtcgtaa
     5281 gggcttggct gcaccggaat cagcacgaag tcggctgcct tgatcgcgga cacagccaag
     5341 tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc gccggccgat ggccttcacg
     5401 tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc ttcccgcacg
     5461 gccgcccaat cgcgggcact gccctgggga tcggaatcga ctaacagaac atcggccccg
     5521 gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga cccgcctttc
     5581 tggttaagta cagcgataac cttcatgcgt tccccttgcg tatttgttta tttactcatc
     5641 gcatcatata cgcagcgacc gcatgacgca agctgtttta ctcaaataca catcaccttt
     5701 ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc gccagcttgg
     5761 catcagacaa accggccagg atttcatgca gccgcacggt tgagacgtgc gcgggcggct
     5821 cgaacacgta cccggccgcg atcatctccg cctcgatctc ttcggtaatg aaaaacggtt
     5881 cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat tctcggcggc
     5941 cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc
     6001 ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc
     6061 aagcagtgca gccgcctctt tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg
     6121 cgcgatctgt gccggggtga gggtagggcg ggggccaaac ttcacgcctc gggccttggc
     6181 ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa cgctcgaact cggcaatgcc
     6241 ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc acggctctgc
     6301 caggctacgc aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg
     6361 ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg
     6421 gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa
     6481 cagcttggtg cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc
     6541 ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg
     6601 tctccggttc tagtcgcaag tattctactt tatgcgacta aaacacgcga caagaaaacg
     6661 ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt
     6721 ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg
     6781 gatcaaagta ctttgatccc gaggggaacc ctgtggttgg catgcacata caaatggacg
     6841 aacggataaa ccttttcacg cccttttaaa tatccgatta ttctaataaa cgctcttttc
     6901 tcttaggttt acccgccaat atatcctgtc aaacactgat agtttaaact gaaggcggga
     6961 aacgacaatc tgatccaagc tcaagctgct ctagcattcg ccattcaggc tgcgcaactg
     7021 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     7081 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     7141 gacggccagt gccaagcttg catgcctgca ggtccccaga ttagcctttt caatttcaga
     7201 aagaatgcta acccacagat ggttagagag gcttacgcag caggtctcat caagacgatc
     7261 tacccgagca ataatctcca ggaaatcaaa taccttccca agaaggttaa agatgcagtc
     7321 aaaagattca ggactaactg catcaagaac acagagaaag atatatttct caagatcaga
     7381 agtactattc cagtatggac gattcaaggc ttgcttcaca aaccaaggca agtaatagag
     7441 attggagtct ctaaaaaggt agttcccact gaatcaaagg ccatggagtc aaagattcaa
     7501 atagaggacc taacagaact cgccgtaaag actggcgaac agttcataca gagtctctta
     7561 cgactcaatg acaagaagaa aatcttcgtc aacatggtgg agcacgacac acttgtctac
     7621 tccaaaaata tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa
     7681 agggtaatat ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg
     7741 aagatagtgg aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc
     7801 atcgttgaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
     7861 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc
     7921 tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata
     7981 taaggaagtt catttcattt ggagagaaca cgggggactc tagagttatc aacaagtttg
     8041 tacaaaaaag caggcttcat gtctgaacat gtggcggaag gagcagtggc ggccgctgta
     8101 aacaacgtgg tggaaaacct gtcgaagctc cccgcggcga catcatcaca tgccgtgggc
     8161 aaatcttcac atggccggct atttaacagg gaaaagacag ttcatcagct acttggcggt
     8221 ggcaaagctg cggacgtcct tttgtggaag cacaagcctt tgtccggtgg tgttctagcg
     8281 ggagcgacaa tcggttggat tctattcgag tggtctggtt acactctact gtccctcgtc
     8341 tcgaacgtgc ttctcttcct cattgtcatc ttcttcttgt ggtcgaatgc tgctggtctt
     8401 ctgaacagag ctccacctcc actacctgaa cttgcgctat ctgaagacac cgtggttcac
     8461 actgcaaaaa ctctccgcgt tgaaatcaac aaaatccttg caatagcaca cgatgttgct
     8521 ctcggaaggg attggaagct ctttctgaag gtggttgccg gcctctgggt gctctctgtt
     8581 attggtggat ggttcaactt cttgacactt gtgtacatcg gtgttgtcgc ctcccatacg
     8641 gtcccagctc tgtatgagaa gtacgaagat gaagttgaca agtacggcaa ggttgcagtt
     8701 gatcaggcta atgtccagta caaaaagatc gatgctctcc tgctgagtaa aatccctagg
     8761 gctgcgccca aggacaagaa ggcgcaggac ccagctttct tgtacaaagt ggttgataac
     8821 agcatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     8881 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     8941 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     9001 accctcgtga ccaccttcgg ctacggcctg atgtgcttcg cccgctaccc cgaccacatg
     9061 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     9121 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     9181 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     9241 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     9301 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     9361 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     9421 cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     9481 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     9541 tgagcttaga gctcgaattt ccccgatcgt tcaaacattt ggcaataaag tttcttaaga
     9601 ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag
     9661 catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga
     9721 gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat
     9781 aaattatcgc gcgcggtgtc atctatgtta ctagatcggg aattggttcc ggaaccaatt
     9841 cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     9901 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     9961 attaggctga attaggcgcg cctatttctg aattcaacac attgcggacg tttttaatgt
    10021 actgaattaa cgccgaatta attcggggga tctggatttt agtactggat tttggtttta
    10081 ggaattagaa attttattga tagaagtatt ttacaaatac aaatacatac taagggtttc
    10141 ttatatgctc aacacatgag cgaaacccta taggaaccct aattccctta tctgggaact
    10201 actcacacat tattatggag aaactcgagt tagtatatag tccttccatc accctctgtg
    10261 ataatctcct taaaacttgc tcccccagga atcataggca gaacgtgctc ctggtgggga
    10321 actatcacat ccagtaaata aggaccggga gaatctagca tagtttgaat tgctgctctt
    10381 agttcatcct tcttcaccac acgcgcattt ggcaccttga aaccgtctgc aactgtacag
    10441 aagtcggggt agatctgctc ctcgtgatct ggatcaccta agtaagtatg tgctctgttg
    10501 gccttgtaga atctatcctc caattgtacg accattccta gatgttggtt gtttaagatc
    10561 atgatcttca cgggtaactt ctccacaaaa attgtagcca actcctgtac gttcatcaca
    10621 aagctaccat cgccatcgat gtcgatcaca ggaatatctg gttgagttgc ggcagccccc
    10681 aaagcagaag gaagtccgaa tcccatggct ccaagacctc cagatgtcaa ccaacgccgc
    10741 ggttcgttgt acttgaacca ctgggctgcc cacatttgat gttggccaac accagtagat
    10801 atgatggcat ttccaccagt gagttcgtac agcgtctgta tggcagattg aggagcaatg
    10861 gcatcctcga atacgggaaa cttcattggc catttctgct tttgttcttc tagttctgcc
    10921 acccagttgg agaagtcgaa agtcctcttt tgactcttca agagggcatt gatttcactt
    10981 agcgcaggtt tcacatcagc acacatggac acatggggct gtttgttctt tccaatttca
    11041 gcagggtcaa tatcgatatg aacgatgctg gacctgctag caaaagtctc cagtttacca
    11101 gtcactcgat cgtcgaaacg cactccaaat gccaacaaca gatctgcttg gtccacggcg
    11161 tagttggcgt aaacagttcc atgcattcca agcattttca aacttaatgg atcagactcg
    11221 ggatacgctc ccaaacccat taaagtcgat gtgataggaa ttccggtaag ctcgacaaat
    11281 tccctcaact cttcgctggc gtgattgcaa cctcctccag aatacactac aggcttctta
    11341 gaactagaaa tgagtcttat aatctgctcg attaacgttg gttccggtgg ccgaggcagt
    11401 cgttgtaagt agcccttcag ttgcattggc tgagaccaat ctggaatagc catttgctgt
    11461 tgcacgtcct tgggaatatc taccaagact gggcccggcc tacctgaagc tgccaaaaag
    11521 aaagcctccc tgattacgcg agggatgtca tcaatgttca taacaaggta attgtgcttg
    11581 gtgatcgatc tcgtaacctc cacaattgga gtttcttgaa aagcatcggt accaatcatc
    11641 ctactggata cttgacctgt aatggccact agtggcacac tgtccaagag ggcatcagcg
    11701 agcccggtaa caagattggt cgctccagga ccggatgtgg ctatacaaac ccctactcgt
    11761 ccagacgatt tagcataacc ttcagcagcg aaaatctccc cctgctcgtg gcgacagaga
    11821 atgttgcgga tgctgctgga acgtgtaaga gcctggtgaa tctccataga cgctccgccg
    11881 ggataagcga acaccgttct aatcccttct cgctcaagag cctccaccaa aatgtcggct
    11941 cctttgcggg gctcgtcatc cttgtagcga gactgaaagt cggacggggt tggtgcaacg
    12001 agtgacttcc cgctgctaag ccgtggtcca ctcacatcga ctgcagactg gatcttctcg
    12061 ctaacagctt gggctttcac ttccagtgaa cgctcagtct tccgagaggt gttgtatgca
    12121 tgtcccctca gagcggctcc cgagaagaaa ccactcctca cctttagctc tttagctccc
    12181 ttgcaacaag accttgccga tggctgcacc ttctccaggc tcgctatgct caaaggcaca
    12241 gctgcgcatc cactaattga cgccatgctc gagagagata gatttgtaga gagagactgg
    12301 tgatttcagc gtgtcctctc caaatgaaat gaacttcctt atatagagga agggtcttgc
    12361 gaaggatagt gggattgtgc gtcatccctt acgtcagtgg agatatcaca tcaatccact
    12421 tgctttgaag acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtggg
//
