LOCUS       sequence-456260-       19100 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The Dual Pol II backbone vector for rice genome editing is based on 
            Mb3Cas12a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19100)
  AUTHORS   Liu S, He Y, Fan T, Zhu M, Qi C, Ma Y, Yang M, Yang L, Tang X, Zhou 
            J, Zhong Z, An X, Qi Y, Zhang Y
  TITLE     PAM-relaxed and temperature-tolerant CRISPR-Mb3Cas12a single 
            transcript unit systems for efficient singular and multiplexed 
            genome editing in rice, maize, and tomato.
  JOURNAL   Plant Biotechnol J. 2024 Oct 10. doi: 10.1111/pbi.14486.
  PUBMED    39387219
REFERENCE   2  (bases 1 to 19100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2024 Oct 10. doi: 10.1111/pbi.14486."
COMMENT     Sequence Label: pGEL1024
FEATURES             Location/Qualifiers
     source          1..19100
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1297..1926
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2355..3428
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3494..3688
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3924..3946)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4032..4172
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4087..4106
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4187..4204)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4358..4946)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4438..4457)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6371..7165)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    7590..7614
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    7692..7866
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(7907..8932)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     CDS             complement(7907..8932)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(8999..9675)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(9042..9062)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    9866..9887
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9902..9907,9908..9925,9926..9932)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9940..9956
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9945..9967
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9964..9980
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9964..9980
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     regulatory      12018..12027
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             12030..12050
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             15855..15902
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      15921..16169
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     ncRNA           17733..17769
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     CDS             complement(17798..18103)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(17900..17919)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     ncRNA           18430..18497
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     primer_bind     complement(18853..18870)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(18853..18869)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(18862..18884)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    19072..19096
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg
       61 ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggatcaa agtactttga
      121 tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg
      181 aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg cccttttcct
      241 ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac tagaaccgga
      301 gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc cgcgtcagca
      361 ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc tgcaccaagc
      421 tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg
      481 accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg gcccgcagca
      541 cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc
      601 tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg accgtgttcg
      661 ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc gggcgcgagg
      721 ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg gcacagatcg
      781 cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc
      841 ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca
      901 ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc gacgccctgg
      961 cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg acggccagga
     1021 cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg ggtacgtgtt
     1081 cgagccgccc gcgcacggct caaccgtgcg gctgcatgaa atcctggccg gtttgtctga
     1141 tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc gccgccgtct
     1201 aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat
     1261 gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc tgtacttaac
     1321 cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc cctgcaactc
     1381 gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg cgattgggcg
     1441 gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac gattgaccgc
     1501 gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg
     1561 gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt gcagccaagc
     1621 ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg cattgaggtc
     1681 acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc
     1741 ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga gtcccgtatc
     1801 acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct tgaatcagaa
     1861 cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa atcaaaactc
     1921 atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta agtgccggcc
     1981 gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca
     2041 tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg
     2101 tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag ctaccagagt
     2161 aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg cggcatggaa
     2221 aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt
     2281 tggccaggcg taagcggctg ggttgcctgc cggccctgca atggcactgg aacccccaag
     2341 cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc
     2401 tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg
     2461 aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat
     2521 cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc
     2581 aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca
     2641 tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct
     2701 acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt
     2761 gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc
     2821 gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca
     2881 agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc
     2941 ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg
     3001 tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg
     3061 ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag
     3121 gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg
     3181 gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt
     3241 tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg
     3301 tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc
     3361 aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg
     3421 gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa
     3481 aaggcctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc
     3541 ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga
     3601 ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa
     3661 ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc caagagctgc
     3721 aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta
     3781 tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg
     3841 gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg
     3901 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggaaac ggtcacagct
     3961 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     4021 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta
     4081 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     4141 acagatgcgt aaggagaaaa taccgcatca ggccctcttc cgcttcctcg ctcactgact
     4201 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     4261 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     4321 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     4381 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     4441 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     4501 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     4561 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     4621 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     4681 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     4741 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     4801 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     4861 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     4921 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     4981 ctcagtgctg agagatcccc tcataatttc cccaaagcgt aaccatgtgt gaataaattt
     5041 tgagctagta gggttgcagc cacgagtaag tcttcccttg ttattgtgta gccagaatgc
     5101 cgcaaaactt ccatgcctaa gcgaactgtt gagagtacgt ttcgatttct gactgtgtta
     5161 gcctggaagt gcttgtccca accttgtttc tgagcatgaa cgcccgcaag ccaacatgtt
     5221 agttgaagca tcagggcgat tagcagcatg atatcaaaac gctctgagct gctcgttcgg
     5281 ctatggcgta ggcctagtcc gtaggcagga cttttcaagt ctcggaaggt ttcttcaatc
     5341 tgcattcgct tcgaatagat attaacaagt tgtttgggtg ttcgaatttc aacaggtaag
     5401 ttagttgcta gaatccatgg ctcctttgcc gacgctgagt agattttagg tgacgggtgg
     5461 tgacaatgag tccgtgtcga gcgctgattt tttcggcctt tagagcgaga tttatacaat
     5521 agaatttggc atgagattgg attgctttta gtcagcctct tatagcctaa agtctttgag
     5581 tgactagatg acatatcatg taagttgctg ataggtttcc agttttccgc tcctaggtct
     5641 gcatattgta cttttcctct tactcgactt aaccagtacc aacccagctt ctcaacggat
     5701 ttataccatg gcactttaaa gccagcatca ctgacaatga gcggtgtggt gttactcggt
     5761 agaatgctcg caaggtcggc tagaaattgg tcatgagctt tctttgaaca ttgctctgaa
     5821 agcgggaacg ctttctcata aagagtaaca gaacgaccgt gtagtgcgac tgaagctcgc
     5881 aataccataa gccgtttttg ctcacggata tcagaccagt caacaagtac aatgggcatc
     5941 gtattgcccg aacagataaa gctagcatgc caacggtata cagcgagtcg ctctttgtgg
     6001 aggtgacgat tacctaacaa tcggtcgatt cgtttgatgt tatgttttgt tctcgctttg
     6061 gttggcaggt tacggccaag ttcggtaaga gtgagagttt tacagtcaag taaggcgtgg
     6121 caagccaacg ttaagctgtt gagtcgtttt aagtgtaatt cggggcagaa ttggtaaaga
     6181 gagtcgtgta aaatatcgag ttcgcacatt ttgttgtctg attattgatt tttggcgaaa
     6241 ccatttgatc atatgacaag atgtgtatct accttaactt aatgattttg ataaaaatca
     6301 ttaggggatt catcagcgct cagtggaacg aaaactcacg ttaagggatt ttggtcatgc
     6361 attctaggta ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag
     6421 gcttgatccc cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc
     6481 tgatcgaccg gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa
     6541 gatcaataaa gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc
     6601 cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc
     6661 gcggatcttt aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt
     6721 tattcagtaa gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat
     6781 ccgatatgtc gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt
     6841 cagggctttg ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga
     6901 gcagattgct ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc
     6961 cttccagcca tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc
     7021 ggctgtccgt catttttaaa tataggtttt cattttctcc caccagctta tataccttag
     7081 caggagacat tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt
     7141 ccggtgatat tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa
     7201 gataccccaa gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta
     7261 aaaccttaaa taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat
     7321 agtatcgacg gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg
     7381 ttacaatcaa catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt
     7441 gccgttcttc cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc
     7501 ccgctgacgc cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc
     7561 tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacaaattg
     7621 acgcttagac aacttaataa cacattgcgg acgtttttaa tgtactgaat taacgccgaa
     7681 ttaattcggg ggatctggat tttagtactg gattttggtt ttaggaatta gaaattttat
     7741 tgatagaagt attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat
     7801 gagcgaaacc ctataggaac cctaattccc ttatctggga actactcaca cattattatg
     7861 gagaaactcg agcttgtcga tcgacagatc ccggtcggca tctactctat ttctttgccc
     7921 tcggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca cagccatcgg
     7981 tccagacggc cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg gctccggatc
     8041 ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca
     8101 agctctgata gagttggtca agaccaatgc ggagcatata cgcccggagt cgtggcgatc
     8161 ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg ctgctccata caagccaacc
     8221 acggcctcca gaagaagatg ttggcgacct cgtattggga atccccgaac atcgcctcgc
     8281 tccagtcaat gaccgctgtt atgcggccat tgtccgtcag gacattgttg gagccgaaat
     8341 ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc ccaaagcatc agctcatcga
     8401 gagcctgcgc gacggacgca ctgacggtgt cgtccatcac agtttgccag tgatacacat
     8461 ggggatcagc aatcgcgcat atgaaatcac gccatgtagt gtattgaccg attccttgcg
     8521 gtccgaatgg gccgaacccg ctcgtctggc taagatcggc cgcagcgatc gcatccatgg
     8581 cctccgcgac cggctgcaga acagcgggca gttcggtttc aggcaggtct tgcaacgtga
     8641 caccctgtgc acggcgggag atgcaatagg tcaggctctc gctgaattcc ccaatgtcaa
     8701 gcacttccgg aatcgggagc gcggccgatg caaagtgccg ataaacataa cgatctttgt
     8761 agaaaccatc ggcgcagcta tttacccgca ggacatatcc acgccctcct acatcgaagc
     8821 tgaaagcacg agattcttcg ccctccgaga gctgcatcag gtcggacacg ctgtcgaact
     8881 tttcgatcag aaacttctcg acagacgtcg cggtgagttc aggctttttc atatctcatt
     8941 gccccccgga tctgcgaaag ctcgagagag atagatttgt agagagagac tggtgatttc
     9001 agcgtgtcct ctccaaatga aatgaacttc cttatataga ggaagggtct tgcgaaggat
     9061 agtgggattg tgcgtcatcc cttacgtcag tggagatatc acatcaatcc acttgctttg
     9121 aagacgtggt tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt
     9181 tgggaccact gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc
     9241 atttgtaggt gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc
     9301 aatggaatcc gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt
     9361 ggtcttctga gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca
     9421 tgttcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg
     9481 ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata
     9541 gcctttcctt tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt
     9601 ttgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt
     9661 tgttgaaaag tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag
     9721 tagacgagag tgtcgtgctc caccatgttg gcaagctgct ctagccaata cgcaaaccgc
     9781 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     9841 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     9901 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     9961 acacaggaaa cagctatgac atgattacga attcgagctc ggtacccggg gatcggcgcg
    10021 cctgcagtgc agcgtgaccc ggtcgtgccc ctctctagag ataatgagca ttgcatgtct
    10081 aagttataaa aaattaccac atattttttt tgtcacactt gtttgaagtg cagtttatct
    10141 atctttatac atatatttaa actttactct acgaataata taatctatag tactacaata
    10201 atatcagtgt tttagagaat catataaatg aacagttaga catggtctaa aggacaattg
    10261 agtattttga caacaggact ctacagtttt atctttttag tgtgcatgtg ttctcctttt
    10321 tttttgcaaa tagcttcacc tatataatac ttcatccatt ttattagtac atccatttag
    10381 ggtttagggt taatggtttt tatagactaa tttttttagt acatctattt tattctattt
    10441 tagcctctaa attaagaaaa ctaaaactct attttagttt ttttatttaa taatttagat
    10501 ataaaataga ataaaataaa gtgactaaaa attaaacaaa taccctttaa gaaattaaaa
    10561 aaactaagga aacatttttc ttgtttcgag tagataatgc cagcctgtta aacgccgtcg
    10621 acgagtctaa cggacaccaa ccagcgaacc agcagcgtcg cgtcgggcca agcgaagcag
    10681 acggcacggc atctctgtcg ctgcctctgg acccctctcg agagttccgc tccaccgttg
    10741 gacttgctcc gctgtcggca tccagaaatt gcgtggcgga gcggcagacg tgagccggca
    10801 cggcaggcgg cctcctcctc ctctcacggc accggcagct acgggggatt cctttcccac
    10861 cgctccttcg ctttcccttc ctcgcccgcc gtaataaata gacaccccct ccacaccctc
    10921 tttccccaac ctcgtgttgt tcggagcgca cacacacaca accagatctc ccccaaatcc
    10981 acccgtcggc acctccgctt caaggtacgc cgctcgtcct cccccccccc ctctctacct
    11041 tctctagatc ggcgttccgg tccatggtta gggcccggta gttctacttc tgttcatgtt
    11101 tgtgttagat ccgtgtttgt gttagatccg tgctgctagc gttcgtacac ggatgcgacc
    11161 tgtacgtcag acacgttctg attgctaact tgccagtgtt tctctttggg gaatcctggg
    11221 atggctctag ccgttccgca gacgggatcg atttcatgat tttttttgtt tcgttgcata
    11281 gggtttggtt tgcccttttc ctttatttca atatatgccg tgcacttgtt tgtcgggtca
    11341 tcttttcatg cttttttttg tcttggttgt gatgatgtgg tctggttggg cggtcgttct
    11401 agatcggagt agaattaatt ctgtttcaaa ctacctggtg gatttattaa ttttggatct
    11461 gtatgtgtgt gccatacata ttcatagtta cgaattgaag atgatggatg gaaatatcga
    11521 tctaggatag gtatacatgt tgatgcgggt tttactgatg catatacaga gatgcttttt
    11581 gttcgcttgg ttgtgatgat gtggtgtggt tgggcggtcg ttcattcgtt ctagatcgga
    11641 gtagaatact gtttcaaact acctggtgta tttattaatt ttggaactgt atgtgtgtgt
    11701 catacatctt catagttacg agtttaagat ggatggaaat atcgatctag gataggtata
    11761 catgttgatg tgggttttac tgatgcatat acatgatggc atatgcagca tctattcata
    11821 tgctctaacc ttgagtacct atctattata ataaacaagt atgttttata attattttga
    11881 tcttgatata cttggatgat ggcatatgca gcagctatat gtggattttt ttagccctgc
    11941 cttcatacgc tatttatttg cttggtactg tttcttttgt cgatgctcac cctgttgttt
    12001 ggtgttactt cctgcaggcc gccatggctc ctaagaagaa gcggaaggtt ggtattcacg
    12061 gggtgcctgc ggctctcttc caggacttca cccacctcta cccactgtcc aagacagtgc
    12121 ggttcgagct gaagcctatc ggcaagaccc tcgagcacat tcatgccaag aacttcctca
    12181 atcaggacga gacaatggcc gatatgtacc agaaggtcaa ggcgatcctg gacgattacc
    12241 atagggactt cattgccgat atgatgggcg aggtgaagct caccaagctg gcggagttct
    12301 acgatgtcta cctgaagttc cgcaagaacc caaaggacga tggcctccag aagcagctca
    12361 aggacctcca ggccgtgctg cggaaggaga tcgtcaagcc tattggcaat ggcggcaagt
    12421 acaaggccgg ctacgatagg ctgttcggcg cgaagctctt caaggacggc aaggagctcg
    12481 gcgatctggc caagttcgtg attgcccagg agggagagtc cagccctaag ctcgcccacc
    12541 tggcgcattt cgagaagttc tcaacctact tcacaggctt ccacgacaac aggaagaata
    12601 tgtactctga cgaggataag cacaccgcca tcgcgtaccg gctgattcat gagaacctcc
    12661 cgaggttcat tgataatctc cagatcctgg ccaccattaa gcagaagcat tccgcgctgt
    12721 acgaccagat cattaacgag ctcacagcca gcggcctcga tgtctcactg gcgtctcacc
    12781 tcgacggcta ccataagctc ctgacacagg agggcatcac cgcctacaat acactcctgg
    12841 gcggcatttc aggagaggcc ggcagccgca agatccaggg cattaacgag ctcatcaatt
    12901 cacaccataa ccagcactgc cataagtctg agaggatcgc caagctccgc ccgctgcaca
    12961 agcagattct gtccgatggc atgggcgtgt ccttcctccc aagcaagttc gccgacgata
    13021 gcgaggtgtg ccaggcggtc aacgagttct accggcatta cgccgacgtg ttcgcgaagg
    13081 tccagtccct cttcgatggc ttcgacgatt accagaagga cggcatctac gtcgagtaca
    13141 agaacctcaa tgagctgagc aagcaggcct tcggcgattt cgccctcctg ggcagggtgc
    13201 tggatggata ctacgtcgat gtggtcaatc cggagttcaa cgagcgcttc gccaaggcga
    13261 agaccgataa cgccaaggcg aagctcacaa aggagaagga caagttcatt aagggagtgc
    13321 actcactggc cagcctggag caggccattg agcactacac cgccaggcat gacgatgagt
    13381 cagtgcaggc cggcaagctg ggacagtact tcaagcatgg cctcgccggc gtggacaacc
    13441 caatccagaa gattcacaac aatcatagca caatcaaggg cttcctcgag cgggagcgcc
    13501 cggccggcga gagggccctg ccaaagatca agtccgataa gtctccagag attcggcagc
    13561 tgaaggaact cctggacaac gccctcaatg tcgcccactt cgcgaagctc ctgaccacaa
    13621 agaccacact ccataatcag gatggcaact tctacggcga gttcggcgcg ctgtacgacg
    13681 agctcgccaa gatcgcgacc ctgtacaaca aggtgcgcga ttacctctca cagaagcctt
    13741 tctctaccga gaagtacaag ctcaacttcg gcaatccgac actcctgaat ggctgggacc
    13801 tgaacaagga gaaggataat ttcggcgtca tcctgcagaa ggacggctgc tactacctcg
    13861 ccctcctgga taaggcgcac aagaaggtgt tcgacaacgc cccgaataca ggcaagagcg
    13921 tctaccagaa gatgatctac aagctcctgc cgggcccaaa caagatgctg ccaaaggtgt
    13981 tcttcgcgaa gtccaacctc gattactaca atcctagcgc cgagctcctg gacaagtacg
    14041 cgcagggcac ccacaagaag ggcgacaact tcaatctgaa ggattgccat gccctcatcg
    14101 acttcttcaa ggcgggcatt aataagcacc cggagtggca gcatttcggc ttcaagttct
    14161 ccccaacatc atcttaccag gacctcagcg atttctaccg cgaggtggag ccacagggct
    14221 accaggtgaa gttcgtggac atcaacgccg attacattaa tgagctcgtc gagcagggcc
    14281 agctctacct gttccagatc tacaacaagg atttctcccc aaaggcccac ggcaagccta
    14341 atctccatac cctgtacttc aaggcgctgt tcagcgagga caacctcgtg aatcctatct
    14401 acaagctcaa cggcgaggcc gagattttct accggaaggc gtccctggat atgaacgaga
    14461 caacaattca cagggccggc gaggtgctcg agaacaagaa tcctgacaac ccgaagaagc
    14521 gccagttcgt ctacgacatc attaaggata agcggtacac acaggataag ttcatgctcc
    14581 atgtgccgat caccatgaat ttcggcgtcc agggcatgac aattaaggag ttcaataaga
    14641 aggtgaacca gtcaatccag cagtacgatg aggtgaacgt cattggaatt gatcgcggcg
    14701 agaggcacct cctgtacctc accgtcatca attctaaggg cgagattctg gagcagaggt
    14761 cactcaacga tatcaccaca gcctctgcga atggcacaca gatgaccaca ccataccata
    14821 agatcctgga caagagggag attgagaggc tcaatgcccg ggtgggatgg ggcgagatcg
    14881 agacaatcaa ggagctgaag tcaggctacc tctctcacgt ggtccatcag atcagccagc
    14941 tcatgctgaa gtacaacgcc attgtggtcc tcgaggacct gaatttcggc ttcaagcggg
    15001 gcaggttcaa ggtggagaag cagatctacc agaacttcga gaatgcgctg attaagaagc
    15061 tcaaccacct cgtcctgaag gataaggccg acgatgagat cggctcatac aagaatgcgc
    15121 tccagctgac caacaatttc acagacctga agtctatcgg caagcagacc ggcttcctct
    15181 tctacgtgcc tgcctggaac acctcaaaga ttgatccgga gacaggcttc gtggacctcc
    15241 tgaagcctcg ctacgagaac attgcccagt ctcaggcgtt cttcggcaag ttcgataaga
    15301 tctgctacaa tgcggaccgg ggctacttcg agttccacat tgattacgcc aagttcaacg
    15361 acaaggcgaa gaattccagg cagatctgga agatttgcag ccatggcgat aagcgctacg
    15421 tgtacgacaa gaccgccaac cagaataagg gcgcgacaat cggcgtgaac gtcaatgatg
    15481 agctcaagtc actgttcacc cggtaccaca ttaacgacaa gcagccaaat ctcgtcatgg
    15541 atatctgcca gaacaatgac aaggagttcc ataagtctct gatgtacctc ctgaagacac
    15601 tcctggccct caggtactca aacgcctcca gcgacgagga tttcatcctc tctcctgtgg
    15661 ccaacgatga gggcgtcttc ttcaattcag cactggccga tgatacacag ccacagaatg
    15721 cggatgccaa cggcgcatac cacattgccc tcaagggcct gtggctcctg aatgagctca
    15781 agaactcaga cgatctgaac aaggtgaagc tcgccattga caatcagaca tggctgaatt
    15841 tcgcgcagaa ccgcaagcgt cctgctgcca ccaaaaaggc cggacaggct aagaaaaaga
    15901 agtgaacgcg tactcgagta tatgaagatg aagatgaaat atttggtgtg tcaaataaaa
    15961 agcttgtgtg cttaagtttg tgtttttttc ttggcttgtt gtgttatgaa tttgtggctt
    16021 tttctaatat taaatgaatg taagatcaca ttataatgaa taaacaaatg tttctataat
    16081 ccattgtgaa tgttttgttg gatctcttct gcagcatata actactgtat gtgctatggt
    16141 atggactatg gaatatgatt aaagataagg agctcggacg gcgcgcccac atcagtctct
    16201 gcacaaagtg catcctgggc tgcttcaatt ataaagcccc attcaccaca tttgctagat
    16261 agtcgaaaag caccatcaat attgagcttc aggtattttt ggttgtgttg tggttggatt
    16321 gattctaata tataccaaat caatataatt cactaccaaa atataccata gccatcacaa
    16381 ctttattaat tttggtagct taagatggta tatataataa ccaattaaca actgattcta
    16441 attttactac ggcccagtat ctaccaatac aaaacaacga gtatgttttc ttccgtcgta
    16501 atcgtacaca gtacaaaaaa acctggccag cctttcttgg gctggggctc tctttcgaaa
    16561 ggtcacaaaa cgtacacggc agtaacgccg cttcgctgcg tgttaacggc caccaacccc
    16621 gccgtgagca aacggcatca gctttccacc tcctcgatat ctccgcggcg ccgtctggac
    16681 ccgccccctt tccgttcctt tctttccttc tcgcgtttgc gtggtgggga cggactcccc
    16741 aaaccgcctc tccctctctt tatttgtcta tattctcact gggccccacc caccgcaccc
    16801 ctgggcccac tcacgagtcc ccccctcccc acctataaat accccacccc ctcctcgcct
    16861 cttcctccat caatcgaatc cccaaaatcg cagagaaaaa aaaatctccc ctcgaagcga
    16921 agcgtcgaat cgccttctca aggtatgcga ttttctgatc ctctccgttc ctcgcgtttg
    16981 atttgatttc ccggcctgtt cgtgattgtg agatgttgtt gttagtctcc gttttgcgat
    17041 ctgtggtaga tttgaacagg gttagatggg gttcgcgtgg tatgctggat ctgtgattat
    17101 gagcgatgct gttcgtggtc caagtattta ttggttcgga tctagaagta gaactgtgct
    17161 agggttgtga tttgttccga tctgttcaat cagtaggatt tagtctctgt ttttctcgtt
    17221 gatccaagta gcagcttcag gtatattttg cttaggttgt ttttgattca gtccctctag
    17281 ttgcatagat tctactctgt tcatgtttaa tctaagggct gcgtcttgtt gattagtgat
    17341 tacatagcat agctttcagg atattttact tgcttatgcc tatcttatca actgttgcac
    17401 ctgtaaattc tagcctatgt taattaacct gccttatgtg ctctcgggat agtgctagta
    17461 gttattgaat cagtttgccg atggaattct agtagttcat agacctgcag attatttttg
    17521 tgaactcgag cacggtgcga ctctctattt tgttaggtca ctgttggtgt tgataggtac
    17581 actgatgtta ttgtggttta gatcgtgtat ctaacatatt ggaataattt gattgactga
    17641 tttctgctgt acttgcttgg tattgttata atttcatgtt catagttgct gaccatgctt
    17701 cggtaattgt gtgtgcagcc tgcaggaaat tactgatgag tccgtgagga cgaaacgagt
    17761 aagctcgtct aatttctact gtttgtagat ggagacctta tattccccag aacatcaggt
    17821 taatggcgtt tttgatgtca ttttcgcggt ggctgagatc agccacttct tccccgataa
    17881 cggaaaccgg cacactggcc atatcggtgg tcatcatgcg ccagctttca tccccgatat
    17941 gcaccaccgg gtaaagttca cgggagactt tatctgacag cagacgtgca ctggccaggg
    18001 ggatcaccat ccgtcgcccg ggcgtgtcaa taatatcact ctgtacatcc acaaacagac
    18061 gataacggct ctctctttta taggtgtaaa ccttaaactg catttcacca gcccctgttc
    18121 tcgtcagcaa aagagccgtt catttcaata aaccgggcga cctcagccat cccttcctga
    18181 ttttccgctt tccagcgttc ggcacgcaga cgacgggctt cattctgcat ggttgtgctt
    18241 accagaccgg agatattgac atcatatatg ccttgagcaa ctgatagctg tcgctgtcaa
    18301 ctgtcactgt aatacgctgc ttcatagcat acctcttttt gacatacttc gggtatacat
    18361 atcagtatat attcttatac cgcaaaaatc agcgcgcaaa tacgcatact gttatctggc
    18421 ttggtctcag gccggcatgg tcccagcctc ctcgctggcg ccggctgggc aacatgcttc
    18481 ggcatggcga atgggacact cgagtcctag acttgtccat cttctggatt ggccaagtta
    18541 attaatgtat gaaataaaag gatgcacaca tagtgacatg ctaatcacta taatgtgggc
    18601 atcaaagttg tgtgttatgt gtaattacta attatctgaa taagagaaag agatcatcca
    18661 tatttcttat cctaaatgaa tgtcacgtgt ctttataatt ctttgatgaa ccagatgcat
    18721 tttattaacc aattccatat acatataaat attaatcata tataattaat atcaattggg
    18781 ttagcaaaac aaatctagtc taggtgtgtt ttgcgagctc ccggggcgcg ccgatatcga
    18841 gctcagtgtg gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
    18901 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
    18961 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgct agagcagctt
    19021 gagcttggat cagattgtcg tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat
    19081 atattggcgg gtaaacctaa
//
