LOCUS       sequence-456264-       15428 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The STU backbone vector for rice genome editing is based on 
            Mb3Cas12a-RRR (D172R/N573R/K579R).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15428)
  AUTHORS   Liu S, He Y, Fan T, Zhu M, Qi C, Ma Y, Yang M, Yang L, Tang X, Zhou 
            J, Zhong Z, An X, Qi Y, Zhang Y
  TITLE     PAM-relaxed and temperature-tolerant CRISPR-Mb3Cas12a single 
            transcript unit systems for efficient singular and multiplexed 
            genome editing in rice, maize, and tomato.
  JOURNAL   Plant Biotechnol J. 2024 Oct 10. doi: 10.1111/pbi.14486.
  PUBMED    39387219
REFERENCE   2  (bases 1 to 15428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2024 Oct 10. doi: 10.1111/pbi.14486."
COMMENT     Sequence Label: pGEL1027
FEATURES             Location/Qualifiers
     source          1..15428
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    195..219
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(240..488)
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     ncRNA           complement(503..570)
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     CDS             897..1202
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1081..1100
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     ncRNA           complement(1230..1266)
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     CDS             complement(1371..1418)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(5226..5246)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      5249..5258
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        7335..8011
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     7948..7968
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     polyA_signal    9101..9275
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    9353..9377
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             9802..10596
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      10683..11271
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11172..11191
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11425..11442
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    11457..11597
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(11523..11542)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11673..11695
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      11931..12125
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(12191..13264)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(13693..14322)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc cgactgcact atagcagcgg
       61 aggggttgga tcaaagtact ttgatcccga ggggaaccct gtggttggca tgcacataca
      121 aatggacgaa cggataaacc ttttcacgcc cttttaaata tccgattatt ctaataaacg
      181 ctcttttctc ttaggtttac ccgccaatat atcctgtcaa acactgatag tttgagctcc
      241 ttatctttaa tcatattcca tagtccatac catagcacat acagtagtta tatgctgcag
      301 aagagatcca acaaaacatt cacaatggat tatagaaaca tttgtttatt cattataatg
      361 tgatcttaca ttcatttaat attagaaaaa gccacaaatt cataacacaa caagccaaga
      421 aaaaaacaca aacttaagca cacaagcttt ttatttgaca caccaaatat ttcatcttca
      481 tcttcatatt ctagactcga gtgtcccatt cgccatgccg aagcatgttg cccagccggc
      541 gccagcgagg aggctgggac catgccggcc tgagaccaag ccagataaca gtatgcgtat
      601 ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca
      661 aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt
      721 tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga
      781 agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt
      841 cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc
      901 agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga
      961 gtgatattat tgacactccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc
     1021 tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc
     1081 gcatgatgac caccgatatg gccagtgtgc cggtttccgt tatcggggaa gaagtggctg
     1141 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat
     1201 aaggtctcca tctaccatag tagaaattag acgagcttac tcgtttcgtc ctcacggact
     1261 catcagtaat tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
     1321 tttttttttt tttttttttt tttttttttt tttttttttt ttttttttca cttctttttc
     1381 ttagcctgtc cggccttttt ggtggcagca ggacgcttgc ggttctgcgc gaaattcagc
     1441 catgtctgat tgtcaatggc gagcttcacc ttgttcagat cgtctgagtt cttgagctca
     1501 ttcaggagcc acaggccctt gagggcaatg tggtatgcgc cgttggcatc cgcattctgt
     1561 ggctgtgtat catcggccag tgctgaattg aagaagacgc cctcatcgtt ggccacagga
     1621 gagaggatga aatcctcgtc gctggaggcg tttgagtacc tgagggccag gagtgtcttc
     1681 aggaggtaca tcagagactt atggaactcc ttgtcattgt tctggcagat atccatgacg
     1741 agatttggct gcttgtcgtt aatgtggtac cgggtgaaca gtgacttgag ctcatcattg
     1801 acgttcacgc cgattgtcgc gcccttattc tggttggcgg tcttgtcgta cacgtagcgc
     1861 ttatcgccat ggctgcaaat cttccagatc tgcctggaat tcttcgcctt gtcgttgaac
     1921 ttggcgtaat caatgtggaa ctcgaagtag ccccggtccg cattgtagca gatcttatcg
     1981 aacttgccga agaacgcctg agactgggca atgttctcgt agcgaggctt caggaggtcc
     2041 acgaagcctg tctccggatc aatctttgag gtgttccagg caggcacgta gaagaggaag
     2101 ccggtctgct tgccgataga cttcaggtct gtgaaattgt tggtcagctg gagcgcattc
     2161 ttgtatgagc cgatctcatc gtcggcctta tccttcagga cgaggtggtt gagcttctta
     2221 atcagcgcat tctcgaagtt ctggtagatc tgcttctcca ccttgaacct gccccgcttg
     2281 aagccgaaat tcaggtcctc gaggaccaca atggcgttgt acttcagcat gagctggctg
     2341 atctgatgga ccacgtgaga gaggtagcct gacttcagct ccttgattgt ctcgatctcg
     2401 ccccatccca cccgggcatt gagcctctca atctccctct tgtccaggat cttatggtat
     2461 ggtgtggtca tctgtgtgcc attcgcagag gctgtggtga tatcgttgag tgacctctgc
     2521 tccagaatct cgcccttaga attgatgacg gtgaggtaca ggaggtgcct ctcgccgcga
     2581 tcaattccaa tgacgttcac ctcatcgtac tgctggattg actggttcac cttcttattg
     2641 aactccttaa ttgtcatgcc ctggacgccg aaattcatgg tgatcggcac atggagcatg
     2701 aacttatcct gtgtgtaccg cttatcctta atgatgtcgt agacgaactg gcgcttcttc
     2761 gggttgtcag gattcttgtt ctcgagcacc tcgccggccc tgtgaattgt tgtctcgttc
     2821 atatccaggg acgccttccg gtagaaaatc tcggcctcgc cgttgagctt gtagatagga
     2881 ttcacgaggt tgtcctcgct gaacagcgcc ttgaagtaca gggtatggag attaggcttg
     2941 ccgtgggcct ttggggagaa atccttgttg tagatctgga acaggtagag ctggccctgc
     3001 tcgacgagct cattaatgta atcggcgttg atgtccacga acttcacctg gtagccctgt
     3061 ggctccacct cgcggtagaa atcgctgagg tcctggtaag atgatgttgg ggagaacttg
     3121 aagccgaaat gctgccactc cgggtgctta ttaatgcccg ccttgaagaa gtcgatgagg
     3181 gcatggcaat ccttcagatt gaagttgtcg cccttcttgt gggtgccctg cgcgtacttg
     3241 tccaggagct cggcgctagg attgtagtaa tcgaggttgg acttcgcgaa gaacaccttt
     3301 ggcagcatct tgtttgggcc cggcaggagc ttgtagatca tcttctggta gacgctcttg
     3361 cctgtattcg gggcgttgtc gaacaccttc ttgtgcgcct tatccaggag ggcgaggtag
     3421 tagcagccgt ccttctgcag gatgacgccg aaattatcct tctccctgtt caggtcccag
     3481 ccccgcagga gtgtcggatt gccgaagttg agcttgtact tctcggtaga gaaaggcttc
     3541 tgtgagaggt aatcgcgcac cttgttgtac agggtcgcga tcttggcgag ctcgtcgtac
     3601 agcgcgccga actcgccgta gaagttgcca tcctgattat ggagtgtggt ctttgtggtc
     3661 aggagcttcg cgaagtgggc gacattgagg gcgttgtcca ggagttcctt cagctgccga
     3721 atctctggag acttatcgga cttgatcttt ggcagggccc tctcgccggc cgggcgctcc
     3781 cgctcgagga agcccttgat tgtgctatga ttgttgtgaa tcttctggat tgggttgtcc
     3841 acgccggcga ggccatgctt gaagtactgt cccagcttgc cggcctgcac tgactcatcg
     3901 tcatgcctgg cggtgtagtg ctcaatggcc tgctccaggc tggccagtga gtgcactccc
     3961 ttaatgaact tgtccttctc ctttgtgagc ttcgccttgg cgttatcggt cttcgccttg
     4021 gcgaagcgct cgttgaactc cggattgacc acatcgacgt agtatccatc cagcaccctg
     4081 cccaggaggg cgaaatcgcc gaaggcctgc ttgctcagct cattgaggtt cttgtactcg
     4141 acgtagatgc cgtccttctg gtaatcgtcg aagccatcga agagggactg gaccttcgcg
     4201 aacacgtcgg cgtaatgccg gtagaactcg ttgaccgcct ggcacacctc gctatcgtcg
     4261 gcgaacttgc ttgggaggaa ggacacgccc atgccatcgg acagaatctg cttgtgcagc
     4321 gggcggagct tggcgatcct ctcagactta tggcagtgct ggttatggtg tgaattgatg
     4381 agctcgttaa tgccctggat cttgcggctg ccggcctctc ctgaaatgcc gcccaggagt
     4441 gtattgtagg cggtgatgcc ctcctgtgtc aggagcttat ggtagccgtc gaggtgagac
     4501 gccagtgaga catcgaggcc gctggctgtg agctcgttaa tgatctggtc gtacagcgcg
     4561 gaatgcttct gcttaatggt ggccaggatc tggagattat caatgaacct cgggaggttc
     4621 tcatgaatca gccggtacgc gatggcggtg tgcttatcct cgtcagagta catattcttc
     4681 ctgttcctgt ggaagcctgt gaagtaggtt gagaacttct cgaaatgcgc caggtgggcg
     4741 agcttagggc tggactctcc ctcctgggca atcacgaact tggccagatc gccgagctcc
     4801 ttgccgtcct tgaagagctt cgcgccgaac agcctatcgt agccggcctt gtacttgccg
     4861 ccattgccaa taggcttgac gatctccttc cgcagcacgg cctggaggtc cttgagctgc
     4921 ttctggaggc catcgtcctt tgggttcttg cggaacttca ggtagacatc gtagaactcc
     4981 gccagcttgg tgagcttcac ctcgcccatc atatcggcaa tgaagtccct atggtaatcg
     5041 tccaggatcg ccttgacctt ctggtacata tcggccattg tctcgtcctg attgaggaag
     5101 ttcttggcat gaatgtgctc gagggtcttg ccgataggct tcagctcgaa ccgcactgtc
     5161 ttggacagtg ggtagaggtg ggtgaagtcc tggaagagca tagccgcagg caccccgtga
     5221 ataccaacct tccgcttctt cttaggagcc atggcggcct gcaggaagta acaccaaaca
     5281 acagggtgag catcgacaaa agaaacagta ccaagcaaat aaatagcgta tgaaggcagg
     5341 gctaaaaaaa tccacatata gctgctgcat atgccatcat ccaagtatat caagatcaaa
     5401 ataattataa aacatacttg tttattataa tagataggta ctcaaggtta gagcatatga
     5461 atagatgctg catatgccat catgtatatg catcagtaaa acccacatca acatgtatac
     5521 ctatcctaga tcgatatttc catccatctt aaactcgtaa ctatgaagat gtatgacaca
     5581 cacatacagt tccaaaatta ataaatacac caggtagttt gaaacagtat tctactccga
     5641 tctagaacga atgaacgacc gcccaaccac accacatcat cacaaccaag cgaacaaaaa
     5701 gcatctctgt atatgcatca gtaaaacccg catcaacatg tatacctatc ctagatcgat
     5761 atttccatcc atcatcttca attcgtaact atgaatatgt atggcacaca catacagatc
     5821 caaaattaat aaatccacca ggtagtttga aacagaatta attctactcc gatctagaac
     5881 gaccgcccaa ccagaccaca tcatcacaac caagacaaaa aaaagcatga aaagatgacc
     5941 cgacaaacaa gtgcacggca tatattgaaa taaaggaaaa gggcaaacca aaccctatgc
     6001 aacgaaacaa aaaaaatcat gaaatcgatc ccgtctgcgg aacggctaga gccatcccag
     6061 gattccccaa agagaaacac tggcaagtta gcaatcagaa cgtgtctgac gtacaggtcg
     6121 catccgtgta cgaacgctag cagcacggat ctaacacaaa cacggatcta acacaaacat
     6181 gaacagaagt agaactaccg ggccctaacc atggaccgga acgccgatct agagaaggta
     6241 gagagggggg ggggggagga cgagcggcgt accttgaagc ggaggtgccg acgggtggat
     6301 ttgggggaga tctggttgtg tgtgtgtgcg ctccgaacaa cacgaggttg gggaaagagg
     6361 gtgtggaggg ggtgtctatt tattacggcg ggcgaggaag ggaaagcgaa ggagcggtgg
     6421 gaaaggaatc ccccgtagct gccggtgccg tgagaggagg aggaggccgc ctgccgtgcc
     6481 ggctcacgtc tgccgctccg ccacgcaatt tctggatgcc gacagcggag caagtccaac
     6541 ggtggagcgg aactctcgag aggggtccag aggcagcgac agagatgccg tgccgtctgc
     6601 ttcgcttggc ccgacgcgac gctgctggtt cgctggttgg tgtccgttag actcgtcgac
     6661 ggcgtttaac aggctggcat tatctactcg aaacaagaaa aatgtttcct tagttttttt
     6721 aatttcttaa agggtatttg tttaattttt agtcacttta ttttattcta ttttatatct
     6781 aaattattaa ataaaaaaac taaaatagag ttttagtttt cttaatttag aggctaaaat
     6841 agaataaaat agatgtacta aaaaaattag tctataaaaa ccattaaccc taaaccctaa
     6901 atggatgtac taataaaatg gatgaagtat tatataggtg aagctatttg caaaaaaaaa
     6961 ggagaacaca tgcacactaa aaagataaaa ctgtagagtc ctgttgtcaa aatactcaat
     7021 tgtcctttag accatgtcta actgttcatt tatatgattc tctaaaacac tgatattatt
     7081 gtagtactat agattatatt attcgtagag taaagtttaa atatatgtat aaagatagat
     7141 aaactgcact tcaaacaagt gtgacaaaaa aaatatgtgg taatttttta taacttagac
     7201 atgcaatgct cattatctct agagaggggc acgaccgggt cacgctgcac tgcaggcgcg
     7261 cccatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa
     7321 gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc
     7381 cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg tggcacctac
     7441 aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctgc cgacagtggt
     7501 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg
     7561 tcttcaaagc aagtggattg atgtgaacat ggtggagcac gacactctcg tctactccaa
     7621 gaatatcaaa gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt
     7681 aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac
     7741 agtagaaaag gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt
     7801 tcaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt
     7861 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac
     7921 tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg
     7981 aagttcattt catttggaga ggacacgctg aaatcaccag tctctctcta caaatctatc
     8041 tctcctagga tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa
     8101 aagttcgaca gcgtgtccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc
     8161 agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc
     8221 tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg
     8281 cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt
     8341 gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag
     8401 gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga
     8461 ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc
     8521 catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct
     8581 ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg
     8641 gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg
     8701 agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg
     8761 tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca
     8821 ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc
     8881 ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc
     8941 cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg
     9001 accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg
     9061 ggatcttgga ggtgattaat taacgatcga caagctctat tttctccata ataatgtgtg
     9121 agtagttccc agataaggga attagggttc ctatagggtt tcgctcatgt gttgagcata
     9181 taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat
     9241 tcctaaaacc aaaatccagt actaaaatcc agatcccccg aattaattcg gcgttaattc
     9301 agtacattaa aaacgtccgc aatgtgttat taagttgtct aagcgtcaat ttgtttacac
     9361 cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc ggcacaaaat
     9421 caccactcga tacaggcagc ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa
     9481 ggcggcagac tttgctcatg ttaccgatgc tattcggaag aacggcaact aagctgccgg
     9541 gtttgaaaca cggatgatct cgcggagggt agcatgttga ttgtaacgat gacagagcgt
     9601 tgctgcctgt gatcaccgcg gtttcaaaat cggctccgtc gatactatgt tatacgccaa
     9661 ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga atgcaaggaa
     9721 cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta aatactgtag
     9781 aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt gaaaaaactg
     9841 atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa ggtatataag
     9901 ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta taaagggacc
     9961 acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa gctgcctgtt
    10021 ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat gagtgaggcc
    10081 gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa gattatcgag
    10141 ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg tccctatacg
    10201 aatagcttag acagccgctt agccgaattg gattacttac tgaataacga tctggccgat
    10261 gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga gctgtatgat
    10321 tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga cctgggagac
    10381 agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg gagaagcggc
    10441 agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga ggatatcggg
    10501 gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga ttgggagaaa
    10561 ataaaatatt atattttact ggatgaattg ttttagtacc tagaatgcat gaccaaaatc
    10621 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
    10681 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
    10741 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
    10801 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
    10861 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
    10921 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
    10981 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
    11041 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
    11101 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
    11161 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
    11221 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
    11281 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
    11341 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
    11401 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
    11461 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
    11521 agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg
    11581 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgctt gtctgctccc
    11641 ggcatccgct tacagacaag ctgtgaccgt ttccgggagc tgcatgtgtc agaggttttc
    11701 accgtcatca ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg
    11761 gcgacggcgc ggcttgtccg cgccctggta gattgcctgg ccgtaggcca gccatttttg
    11821 agcggccagc ggccgcgata ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc
    11881 gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg gccagacagt tatgcacagg
    11941 ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt
    12001 ttttctcttt tatatcagtc acttacatgt gtgaccggtt cccaatgtac ggctttgggt
    12061 tcccaatgta cgggttccgg ttcccaatgt acggctttgg gttcccaatg tacgtgctat
    12121 ccacaggaaa gaggcctttt cgaccttttt cccctgctag ggcaatttgc cctagcatct
    12181 gctccgtaca ttaggaaccg gcggatgctt cgccctcgat caggttgcgg tagcgcatga
    12241 ctaggatcgg gccagcctgc cccgcctcct ccttcaaatc gtactccggc aggtcatttg
    12301 acccgatcag cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg ctgccactgc
    12361 gttcgtagat cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca
    12421 ggcggtagag aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca
    12481 gcacgtccgg gttcttgcct tctgtgatct cgcggtacat ccaatcagct agctcgatct
    12541 cgatgtactc cggccgcccg gtttcgctct ttacgatctt gtagcggcta atcaaggctt
    12601 caccctcgga taccgtcacc aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa
    12661 cgtgcgtggt gtttaaccga atgcaggttt ctaccaggtc gtctttctgc tttccgccat
    12721 cggctcgccg gcagaacttg agtacgtccg caacgtgtgg acggaacacg cggccgggct
    12781 tgtctccctt cccttcccgg tatcggttca tggattcggt tagatgggaa accgccatca
    12841 gtaccaggtc gtaatcccac acactggcca tgccggccgg ccctgcggaa acctctacgt
    12901 gcccgtctgg aagctcgtag cggatcacct cgccagctcg tcggtcacgc ttcgacagac
    12961 ggaaaacggc cacgtccatg atgctgcgac tatcgcgggt gcccacgtca tagagcatcg
    13021 gaacgaaaaa atctggttgc tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg
    13081 ctgccggcgg ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc cacgattcac
    13141 cggggcgtgc ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct
    13201 ccaccaggtc atcacccagc gccgcgccga tttgtaccgg gccggatggt ttgcgaccgc
    13261 tcacgccgat tcctcgggct tgggggttcc agtgccattg cagggccggc aggcaaccca
    13321 gccgcttacg cctggccaac cgcccgttcc tccacacatg gggcattcca cggcgtcggt
    13381 gcctggttgt tcttgatttt ccatgccgcc tcctttagcc gctaaaattc atctactcat
    13441 ttattcattt gctcatttac tctggtagct gcgcgatgta ttcagatagc agctcggtaa
    13501 tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc gccggcaact
    13561 gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc
    13621 tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt gcttttgctc attttctctt
    13681 tacctcatta actcaaatga gttttgattt aatttcagcg gccagcgcct ggacctcgcg
    13741 ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg
    13801 gtagctcacg cgctgcgtga tacgggactc aagaatgggc agctcgtacc cggccagcgc
    13861 ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc
    13921 ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc agctccacca ggtcggcggt
    13981 ggcccatatg tcgtaagggc ttggctgcac cggaatcagc acgaagtcgg ctgccttgat
    14041 cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg atcactacga agtcgcgccg
    14101 gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg
    14161 ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc tggggatcgg aatcgactaa
    14221 cagaacatcg gccccggcga gttgcagggc gcgggctaga tgggttgcga tggtcgtctt
    14281 gcctgacccg cctttctggt taagtacagc gataaccttc atgcgttccc cttgcgtatt
    14341 tgtttattta ctcatcgcat catatacgca gcgaccgcat gacgcaagct gttttactca
    14401 aatacacatc acctttttag acggcggcgc tcggtttctt cagcggccaa gctggccggc
    14461 caggccgcca gcttggcatc agacaaaccg gccaggattt catgcagccg cacggttgag
    14521 ccgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca tctccgcctc gatctcttcg
    14581 gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc
    14641 gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac
    14701 cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg
    14761 tcgagcgatg cacgccaagc agtgcagccg cctctttcac ggtgcggcct tcctggtcga
    14821 tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg ccaaacttca
    14881 cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct
    14941 cgaactcggc aatgccggcg aacacggtca acaccatgcg gccggccggc gtggtggtgt
    15001 cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa
    15061 tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact gtcacaacgt
    15121 cgccagggcg taggtggtca agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg
    15181 tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg
    15241 tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag caggccagcg gcggcgctct
    15301 tgttcatggc gtaatgtctc cggttctagt cgcaagtatt ctactttatg cgactaaaac
    15361 acgcgacaag aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta acttaggact
    15421 tgtgcgac
//
