LOCUS       sequence-456268-       12474 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The STU backbone vector for tomato genome editing is based on 
            Mb3Cas12a-RRR (D172R/N573R/K579R).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12474)
  AUTHORS   Liu S, He Y, Fan T, Zhu M, Qi C, Ma Y, Yang M, Yang L, Tang X, Zhou 
            J, Zhong Z, An X, Qi Y, Zhang Y
  TITLE     PAM-relaxed and temperature-tolerant CRISPR-Mb3Cas12a single 
            transcript unit systems for efficient singular and multiplexed 
            genome editing in rice, maize, and tomato.
  JOURNAL   Plant Biotechnol J. 2024 Oct 10. doi: 10.1111/pbi.14486.
  PUBMED    39387219
REFERENCE   2  (bases 1 to 12474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2024 Oct 10. doi: 10.1111/pbi.14486."
COMMENT     Sequence Label: pGEL1029
FEATURES             Location/Qualifiers
     source          1..12474
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             33..53
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3861..3908
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     ncRNA           4013..4049
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     CDS             complement(4077..4382)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(4179..4198)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     ncRNA           4709..4776
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     terminator      4791..5039
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    5060..5084
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6385..7014
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7443..8516
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8582..8776
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9012..9034)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9110..9250
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9165..9184
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9265..9282)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9436..10024)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9516..9535)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10111..10905)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11330..11354
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    11432..11606
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(11632..12426)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11743..11762)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     12353..12372
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 aacacggggg actcctgcag gccgccatgg ctcctaagaa gaagcggaag gttggtattc
       61 acggggtgcc tgcggctatg ctcttccagg acttcaccca cctctaccca ctgtccaaga
      121 cagtgcggtt cgagctgaag cctatcggca agaccctcga gcacattcat gccaagaact
      181 tcctcaatca ggacgagaca atggccgata tgtaccagaa ggtcaaggcg atcctggacg
      241 attaccatag ggacttcatt gccgatatga tgggcgaggt gaagctcacc aagctggcgg
      301 agttctacga tgtctacctg aagttccgca agaacccaaa ggacgatggc ctccagaagc
      361 agctcaagga cctccaggcc gtgctgcgga aggagatcgt caagcctatt ggcaatggcg
      421 gcaagtacaa ggccggctac gataggctgt tcggcgcgaa gctcttcaag gacggcaagg
      481 agctcggcga tctggccaag ttcgtgattg cccaggaggg agagtccagc cctaagctcg
      541 cccacctggc gcatttcgag aagttctcaa cctacttcac aggcttccac aggaacagga
      601 agaatatgta ctctgacgag gataagcaca ccgccatcgc gtaccggctg attcatgaga
      661 acctcccgag gttcattgat aatctccaga tcctggccac cattaagcag aagcattccg
      721 cgctgtacga ccagatcatt aacgagctca cagccagcgg cctcgatgtc tcactggcgt
      781 ctcacctcga cggctaccat aagctcctga cacaggaggg catcaccgcc tacaatacac
      841 tcctgggcgg catttcagga gaggccggca gccgcaagat ccagggcatt aacgagctca
      901 tcaattcaca ccataaccag cactgccata agtctgagag gatcgccaag ctccgcccgc
      961 tgcacaagca gattctgtcc gatggcatgg gcgtgtcctt cctcccaagc aagttcgccg
     1021 acgatagcga ggtgtgccag gcggtcaacg agttctaccg gcattacgcc gacgtgttcg
     1081 cgaaggtcca gtccctcttc gatggcttcg acgattacca gaaggacggc atctacgtcg
     1141 agtacaagaa cctcaatgag ctgagcaagc aggccttcgg cgatttcgcc ctcctgggca
     1201 gggtgctgga tggatactac gtcgatgtgg tcaatccgga gttcaacgag cgcttcgcca
     1261 aggcgaagac cgataacgcc aaggcgaagc tcacaaagga gaaggacaag ttcattaagg
     1321 gagtgcactc actggccagc ctggagcagg ccattgagca ctacaccgcc aggcatgacg
     1381 atgagtcagt gcaggccggc aagctgggac agtacttcaa gcatggcctc gccggcgtgg
     1441 acaacccaat ccagaagatt cacaacaatc atagcacaat caagggcttc ctcgagcggg
     1501 agcgcccggc cggcgagagg gccctgccaa agatcaagtc cgataagtct ccagagattc
     1561 ggcagctgaa ggaactcctg gacaacgccc tcaatgtcgc ccacttcgcg aagctcctga
     1621 ccacaaagac cacactccat aatcaggatg gcaacttcta cggcgagttc ggcgcgctgt
     1681 acgacgagct cgccaagatc gcgaccctgt acaacaaggt gcgcgattac ctctcacaga
     1741 agcctttctc taccgagaag tacaagctca acttcggcaa tccgacactc ctgcggggct
     1801 gggacctgaa cagggagaag gataatttcg gcgtcatcct gcagaaggac ggctgctact
     1861 acctcgccct cctggataag gcgcacaaga aggtgttcga caacgccccg aatacaggca
     1921 agagcgtcta ccagaagatg atctacaagc tcctgccggg cccaaacaag atgctgccaa
     1981 aggtgttctt cgcgaagtcc aacctcgatt actacaatcc tagcgccgag ctcctggaca
     2041 agtacgcgca gggcacccac aagaagggcg acaacttcaa tctgaaggat tgccatgccc
     2101 tcatcgactt cttcaaggcg ggcattaata agcacccgga gtggcagcat ttcggcttca
     2161 agttctcccc aacatcatct taccaggacc tcagcgattt ctaccgcgag gtggagccac
     2221 agggctacca ggtgaagttc gtggacatca acgccgatta cattaatgag ctcgtcgagc
     2281 agggccagct ctacctgttc cagatctaca acaaggattt ctccccaaag gcccacggca
     2341 agcctaatct ccataccctg tacttcaagg cgctgttcag cgaggacaac ctcgtgaatc
     2401 ctatctacaa gctcaacggc gaggccgaga ttttctaccg gaaggcgtcc ctggatatga
     2461 acgagacaac aattcacagg gccggcgagg tgctcgagaa caagaatcct gacaacccga
     2521 agaagcgcca gttcgtctac gacatcatta aggataagcg gtacacacag gataagttca
     2581 tgctccatgt gccgatcacc atgaatttcg gcgtccaggg catgacaatt aaggagttca
     2641 ataagaaggt gaaccagtca atccagcagt acgatgaggt gaacgtcatt ggaattgatc
     2701 gcggcgagag gcacctcctg tacctcaccg tcatcaattc taagggcgag attctggagc
     2761 agaggtcact caacgatatc accacagcct ctgcgaatgg cacacagatg accacaccat
     2821 accataagat cctggacaag agggagattg agaggctcaa tgcccgggtg ggatggggcg
     2881 agatcgagac aatcaaggag ctgaagtcag gctacctctc tcacgtggtc catcagatca
     2941 gccagctcat gctgaagtac aacgccattg tggtcctcga ggacctgaat ttcggcttca
     3001 agcggggcag gttcaaggtg gagaagcaga tctaccagaa cttcgagaat gcgctgatta
     3061 agaagctcaa ccacctcgtc ctgaaggata aggccgacga tgagatcggc tcatacaaga
     3121 atgcgctcca gctgaccaac aatttcacag acctgaagtc tatcggcaag cagaccggct
     3181 tcctcttcta cgtgcctgcc tggaacacct caaagattga tccggagaca ggcttcgtgg
     3241 acctcctgaa gcctcgctac gagaacattg cccagtctca ggcgttcttc ggcaagttcg
     3301 ataagatctg ctacaatgcg gaccggggct acttcgagtt ccacattgat tacgccaagt
     3361 tcaacgacaa ggcgaagaat tccaggcaga tctggaagat ttgcagccat ggcgataagc
     3421 gctacgtgta cgacaagacc gccaaccaga ataagggcgc gacaatcggc gtgaacgtca
     3481 atgatgagct caagtcactg ttcacccggt accacattaa cgacaagcag ccaaatctcg
     3541 tcatggatat ctgccagaac aatgacaagg agttccataa gtctctgatg tacctcctga
     3601 agacactcct ggccctcagg tactcaaacg cctccagcga cgaggatttc atcctctctc
     3661 ctgtggccaa cgatgagggc gtcttcttca attcagcact ggccgatgat acacagccac
     3721 agaatgcgga tgccaacggc gcataccaca ttgccctcaa gggcctgtgg ctcctgaatg
     3781 agctcaagaa ctcagacgat ctgaacaagg tgaagctcgc cattgacaat cagacatggc
     3841 tgaatttcgc gcagaaccgc aagcgtcctg ctgccaccaa aaaggccgga caggctaaga
     3901 aaaagaagtg aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     3961 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaat tactgatgag
     4021 tccgtgagga cgaaacgagt aagctcgtct aatttctact atggtagatg gagaccttat
     4081 attccccaga acatcaggtt aatggcgttt ttgatgtcat tttcgcggtg gctgagatca
     4141 gccacttctt ccccgataac ggaaaccggc acactggcca tatcggtggt catcatgcgc
     4201 cagctttcat ccccgatatg caccaccggg taaagttcac gggagacttt atctgacagc
     4261 agacgtgcac tggccagggg gatcaccatc cgtcgcccgg gagtgtcaat aatatcactc
     4321 tgtacatcca caaacagacg ataacggctc tctcttttat aggtgtaaac cttaaactgc
     4381 atttcaccag cccctgttct cgtcagcaaa agagccgttc atttcaataa accgggcgac
     4441 ctcagccatc ccttcctgat tttccgcttt ccagcgttcg gcacgcagac gacgggcttc
     4501 attctgcatg gttgtgctta ccagaccgga gatattgaca tcatatatgc cttgagcaac
     4561 tgatagctgt cgctgtcaac tgtcactgta atacgctgct tcatagcata cctctttttg
     4621 acatacttcg ggtatacata tcagtatata ttcttatacc gcaaaaatca gcgcgcaaat
     4681 acgcatactg ttatctggct tggtctcagg ccggcatggt cccagcctcc tcgctggcgc
     4741 cggctgggca acatgcttcg gcatggcgaa tgggacactc gagtctagaa tatgaagatg
     4801 aagatgaaat atttggtgtg tcaaataaaa agcttgtgtg cttaagtttg tgtttttttc
     4861 ttggcttgtt gtgttatgaa tttgtggctt tttctaatat taaatgaatg taagatcaca
     4921 ttataatgaa taaacaaatg tttctataat ccattgtgaa tgttttgttg gatctcttct
     4981 gcagcatata actactgtat gtgctatggt atggactatg gaatatgatt aaagataagg
     5041 agctcaaact atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg
     5101 tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg
     5161 tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc
     5221 gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt
     5281 cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg
     5341 tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca
     5401 tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg
     5461 acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga
     5521 agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc
     5581 ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac
     5641 tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt
     5701 gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg
     5761 agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc
     5821 gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg
     5881 agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc
     5941 gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc
     6001 ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga
     6061 atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt
     6121 cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc
     6181 gcacggctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc
     6241 ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg
     6301 tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa
     6361 taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg
     6421 tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc cggggccgat
     6481 gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa
     6541 gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc
     6601 atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg
     6661 tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata
     6721 tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg
     6781 ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt
     6841 gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg
     6901 agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac
     6961 gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat
     7021 gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac
     7081 gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca
     7141 actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca
     7201 agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat
     7261 gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa
     7321 ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta
     7381 agcggctggg ttgcctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg
     7441 gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc
     7501 tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac
     7561 gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc
     7621 cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt
     7681 tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg
     7741 ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag
     7801 acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc
     7861 tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg
     7921 gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc
     7981 gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca
     8041 cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg
     8101 agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt
     8161 acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg
     8221 acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct
     8281 accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct
     8341 acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga
     8401 tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga
     8461 tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta
     8521 cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggcctctttc
     8581 ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca
     8641 ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag
     8701 agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc
     8761 gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta
     8821 cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg
     8881 gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc
     8941 cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat
     9001 gacggtgaaa acctctgaca catgcagctc ccggaaacgg tcacagcttg tctgtaagcg
     9061 gatgccggga gcagacaagc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
     9121 cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat
     9181 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     9241 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     9301 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     9361 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     9421 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     9481 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     9541 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     9601 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     9661 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     9721 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     9781 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     9841 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     9901 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     9961 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
    10021 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
    10081 ttaagggatt ttggtcatgc attctaggta ctaaaacaat tcatccagta aaatataata
    10141 ttttattttc tcccaatcag gcttgatccc cagtaagtca aaaaatagct cgacatactg
    10201 ttcttccccg atatcctccc tgatcgaccg gacgcagaag gcaatgtcat accacttgtc
    10261 cgccctgccg cttctcccaa gatcaataaa gccacttact ttgccatctt tcacaaagat
    10321 gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt
    10381 taaaaaatca tacagctcgc gcggatcttt aaatggagtg tcttcttccc agttttcgca
    10441 atccacatcg gccagatcgt tattcagtaa gtaatccaat tcggctaagc ggctgtctaa
    10501 gctattcgta tagggacaat ccgatatgtc gatggagtga aagagcctga tgcactccgc
    10561 atacagctcg ataatctttt cagggctttg ttcatcttca tactcttccg agcaaaggac
    10621 gccatcggcc tcactcatga gcagattgct ccagccatca tgccgttcaa agtgcaggac
    10681 ctttggaaca ggcagctttc cttccagcca tagcatcatg tccttttccc gttccacatc
    10741 ataggtggtc cctttatacc ggctgtccgt catttttaaa tataggtttt cattttctcc
    10801 caccagctta tataccttag caggagacat tccttccgta tcttttacgc agcggtattt
    10861 ttcgatcagt tttttcaatt ccggtgatat tctcatttta gccatttatt atttccttcc
    10921 tcttttctac agtatttaaa gataccccaa gaagctaatt ataacaagac gaactccaat
    10981 tcactgttcc ttgcattcta aaaccttaaa taccagaaaa cagctttttc aaagttgttt
    11041 tcaaagttgg cgtataacat agtatcgacg gagccgattt tgaaaccgcg gtgatcacag
    11101 gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc tccgcgagat catccgtgtt
    11161 tcaaacccgg cagcttagtt gccgttcttc cgaatagcat cggtaacatg agcaaagtct
    11221 gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg cctgtatcga
    11281 gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt ggcaggatat
    11341 attgtggtgt aaacaaattg acgcttagac aacttaataa cacattgcgg acgtttttaa
    11401 tgtactgaat taacgccgaa ttaattcggg ggatctggat tttagtactg gattttggtt
    11461 ttaggaatta gaaattttat tgatagaagt attttacaaa tacaaataca tactaagggt
    11521 ttcttatatg ctcaacacat gagcgaaacc ctataggaac cctaattccc ttatctggga
    11581 actactcaca cattattatg gagaaaatag agcttgtcga tcgttaatta atcagaagaa
    11641 ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag
    11701 cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa
    11761 cgctatgtcc tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa
    11821 gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc
    11881 atcgccgtcg ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg
    11941 atgctcttcg tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg
    12001 ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag
    12061 ccgccgcatt gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag
    12121 gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac
    12181 gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc
    12241 gtcctgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc
    12301 ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc
    12361 atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc
    12421 aatcatccta ggagagatag atttgtagag agagactggt gatttcagcg tgtc
//
