LOCUS       sequence-456269-       15350 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The STU backbone vector for rice genome editing is based on 
            Mb3Cas12a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15350)
  AUTHORS   Liu S, He Y, Fan T, Zhu M, Qi C, Ma Y, Yang M, Yang L, Tang X, Zhou 
            J, Zhong Z, An X, Qi Y, Zhang Y
  TITLE     PAM-relaxed and temperature-tolerant CRISPR-Mb3Cas12a single 
            transcript unit systems for efficient singular and multiplexed 
            genome editing in rice, maize, and tomato.
  JOURNAL   Plant Biotechnol J. 2024 Oct 10. doi: 10.1111/pbi.14486.
  PUBMED    39387219
REFERENCE   2  (bases 1 to 15350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2024 Oct 10. doi: 10.1111/pbi.14486."
COMMENT     Sequence Label: pGEL1025
FEATURES             Location/Qualifiers
     source          1..15350
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     164..184
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        173..219
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     polyA_signal    1317..1491
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    1569..1593
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2018..2812
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      2899..3487
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3388..3407
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3641..3658
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3673..3813
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3739..3758)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3889..3911
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4147..4341
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(4407..5480)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(5909..6538)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    7839..7863
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(7884..8132)
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     ncRNA           complement(8147..8214)
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     CDS             8541..8846
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8725..8744
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     ncRNA           complement(8874..8910)
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     CDS             complement(9008..9055)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(12860..12880)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      12883..12892
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac
       61 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt
      121 gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc
      181 cttcctctat ataaggaagt tcatttcatt tggagaggac acgctgaaat caccagtctc
      241 tctctacaaa tctatctctc ctaggatgaa aaagcctgaa ctcaccgcga cgtctgtcga
      301 gaagtttctg atcgaaaagt tcgacagcgt gtccgacctg atgcagctct cggagggcga
      361 agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag
      421 ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct
      481 cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc
      541 ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct
      601 gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg
      661 gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg
      721 cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc
      781 gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg
      841 gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac
      901 agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat
      961 cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag
     1021 gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga
     1081 ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg
     1141 atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag
     1201 aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg
     1261 ccccagcact cgtccgggat cttggaggtg attaattaac gatcgacaag ctctattttc
     1321 tccataataa tgtgtgagta gttcccagat aagggaatta gggttcctat agggtttcgc
     1381 tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat
     1441 caataaaatt tctaattcct aaaaccaaaa tccagtacta aaatccagat cccccgaatt
     1501 aattcggcgt taattcagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc
     1561 gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg
     1621 cagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga cggcgtcagc
     1681 gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt cggaagaacg
     1741 gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt
     1801 aacgatgaca gagcgttgct gcctgtgatc accgcggttt caaaatcggc tccgtcgata
     1861 ctatgttata cgccaacttt gaaaacaact ttgaaaaagc tgttttctgg tatttaaggt
     1921 tttagaatgc aaggaacagt gaattggagt tcgtcttgtt ataattagct tcttggggta
     1981 tctttaaata ctgtagaaaa gaggaaggaa ataataaatg gctaaaatga gaatatcacc
     2041 ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc
     2101 tgctaaggta tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag
     2161 ccggtataaa gggaccacct atgatgtgga acgggaaaag gacatgatgc tatggctgga
     2221 aggaaagctg cctgttccaa aggtcctgca ctttgaacgg catgatggct ggagcaatct
     2281 gctcatgagt gaggccgatg gcgtcctttg ctcggaagag tatgaagatg aacaaagccc
     2341 tgaaaagatt atcgagctgt atgcggagtg catcaggctc tttcactcca tcgacatatc
     2401 ggattgtccc tatacgaata gcttagacag ccgcttagcc gaattggatt acttactgaa
     2461 taacgatctg gccgatgtgg attgcgaaaa ctgggaagaa gacactccat ttaaagatcc
     2521 gcgcgagctg tatgattttt taaagacgga aaagcccgaa gaggaacttg tcttttccca
     2581 cggcgacctg ggagacagca acatctttgt gaaagatggc aaagtaagtg gctttattga
     2641 tcttgggaga agcggcaggg cggacaagtg gtatgacatt gccttctgcg tccggtcgat
     2701 cagggaggat atcggggaag aacagtatgt cgagctattt tttgacttac tggggatcaa
     2761 gcctgattgg gagaaaataa aatattatat tttactggat gaattgtttt agtacctaga
     2821 atgcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     2881 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     2941 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     3001 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     3061 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     3121 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     3181 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     3241 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     3301 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     3361 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     3421 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     3481 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     3541 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     3601 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     3661 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     3721 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact
     3781 ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac
     3841 gcgcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtttcc gggagctgca
     3901 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc ttgatgtggg
     3961 cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt gcctggccgt
     4021 aggccagcca tttttgagcg gccagcggcc gcgataggcc gacgcgaagc ggcggggcgt
     4081 agggagcgca gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt gcgctggcca
     4141 gacagttatg cacaggccag gcgggtttta agagttttaa taagttttaa agagttttag
     4201 gcggaaaaat cgcctttttt ctcttttata tcagtcactt acatgtgtga ccggttccca
     4261 atgtacggct ttgggttccc aatgtacggg ttccggttcc caatgtacgg ctttgggttc
     4321 ccaatgtacg tgctatccac aggaaagagg ccttttcgac ctttttcccc tgctagggca
     4381 atttgcccta gcatctgctc cgtacattag gaaccggcgg atgcttcgcc ctcgatcagg
     4441 ttgcggtagc gcatgactag gatcgggcca gcctgccccg cctcctcctt caaatcgtac
     4501 tccggcaggt catttgaccc gatcagcttg cgcacggtga aacagaactt cttgaactct
     4561 ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc atctggcttc tgccttgcct
     4621 gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat caaaaagtaa
     4681 tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg gtacatccaa
     4741 tcagctagct cgatctcgat gtactccggc cgcccggttt cgctctttac gatcttgtag
     4801 cggctaatca aggcttcacc ctcggatacc gtcaccaggc ggccgttctt ggccttcttc
     4861 gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac caggtcgtct
     4921 ttctgctttc cgccatcggc tcgccggcag aacttgagta cgtccgcaac gtgtggacgg
     4981 aacacgcggc cgggcttgtc tcccttccct tcccggtatc ggttcatgga ttcggttaga
     5041 tgggaaaccg ccatcagtac caggtcgtaa tcccacacac tggccatgcc ggccggccct
     5101 gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc agctcgtcgg
     5161 tcacgcttcg acagacggaa aacggccacg tccatgatgc tgcgactatc gcgggtgccc
     5221 acgtcataga gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg cggcttccta
     5281 atcgacggcg caccggctgc cggcggttgc cgggattctt tgcggattcg atcagcggcc
     5341 gcttgccacg attcaccggg gcgtgcttct gcctcgatgc gttgccgctg ggcggcctgc
     5401 gcggccttca acttctccac caggtcatca cccagcgccg cgccgatttg taccgggccg
     5461 gatggtttgc gaccgctcac gccgattcct cgggcttggg ggttccagtg ccattgcagg
     5521 gccggcaggc aacccagccg cttacgcctg gccaaccgcc cgttcctcca cacatggggc
     5581 attccacggc gtcggtgcct ggttgttctt gattttccat gccgcctcct ttagccgcta
     5641 aaattcatct actcatttat tcatttgctc atttactctg gtagctgcgc gatgtattca
     5701 gatagcagct cggtaatggt cttgccttgg cgtaccgcgt acatcttcag cttggtgtga
     5761 tcctccgccg gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc caggctggcc
     5821 aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt
     5881 ttgctcattt tctctttacc tcattaactc aaatgagttt tgatttaatt tcagcggcca
     5941 gcgcctggac ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg
     6001 cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga atgggcagct
     6061 cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg
     6121 acacgacaaa ggccgcttgt agccttccat ccgtgacctc aatgcgctgc ttaaccagct
     6181 ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga atcagcacga
     6241 agtcggctgc cttgatcgcg gacacagcca agtccgccgc ctggggcgct ccgtcgatca
     6301 ctacgaagtc gcgccggccg atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc
     6361 cgacaacggt tagcggttga tcttcccgca cggccgccca atcgcgggca ctgccctggg
     6421 gatcggaatc gactaacaga acatcggccc cggcgagttg cagggcgcgg gctagatggg
     6481 ttgcgatggt cgtcttgcct gacccgcctt tctggttaag tacagcgata accttcatgc
     6541 gttccccttg cgtatttgtt tatttactca tcgcatcata tacgcagcga ccgcatgacg
     6601 caagctgttt tactcaaata cacatcacct ttttagacgg cggcgctcgg tttcttcagc
     6661 ggccaagctg gccggccagg ccgccagctt ggcatcagac aaaccggcca ggatttcatg
     6721 cagccgcacg gttgagccgt gcgcgggcgg ctcgaacacg tacccggccg cgatcatctc
     6781 cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt gcggtttcat
     6841 gcttgttcct cttggcgttc attctcggcg gccgccaggg cgtcggcctc ggtcaatgcg
     6901 tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc gctgcgctca
     6961 agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg cagccgcctc tttcacggtg
     7021 cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt gagggtaggg
     7081 cgggggccaa acttcacgcc tcgggccttg gcggcctcgc gcccgctccg ggtgcggtcg
     7141 atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac catgcggccg
     7201 gccggcgtgg tggtgtcggc ccacggctct gccaggctac gcaggcccgc gccggcctcc
     7261 tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg gtctagcctg
     7321 gtcactgtca caacgtcgcc agggcgtagg tggtcaagca tcctggccag ctccgggcgg
     7381 tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg tgcagccggc cgcgtgcagt
     7441 tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata gcccagcagg
     7501 ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt tctagtcgca agtattctac
     7561 tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa agggcagggc ggcagcctgt
     7621 cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag acggctgcac tgaacgtcag
     7681 aagccgactg cactatagca gcggaggggt tggatcaaag tactttgatc ccgaggggaa
     7741 ccctgtggtt ggcatgcaca tacaaatgga cgaacggata aaccttttca cgccctttta
     7801 aatatccgat tattctaata aacgctcttt tctcttaggt ttacccgcca atatatcctg
     7861 tcaaacactg atagtttgag ctccttatct ttaatcatat tccatagtcc ataccatagc
     7921 acatacagta gttatatgct gcagaagaga tccaacaaaa cattcacaat ggattataga
     7981 aacatttgtt tattcattat aatgtgatct tacattcatt taatattaga aaaagccaca
     8041 aattcataac acaacaagcc aagaaaaaaa cacaaactta agcacacaag ctttttattt
     8101 gacacaccaa atatttcatc ttcatcttca tattctagac tcgagtgtcc cattcgccat
     8161 gccgaagcat gttgcccagc cggcgccagc gaggaggctg ggaccatgcc ggcctgagac
     8221 caagccagat aacagtatgc gtatttgcgc gctgattttt gcggtataag aatatatact
     8281 gatatgtata cccgaagtat gtcaaaaaga ggtatgctat gaagcagcgt attacagtga
     8341 cagttgacag cgacagctat cagttgctca aggcatatat gatgtcaata tctccggtct
     8401 ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga
     8461 aaatcaggaa gggatggctg aggtcgcccg gtttattgaa atgaacggct cttttgctga
     8521 cgagaacagg ggctggtgaa atgcagttta aggtttacac ctataaaaga gagagccgtt
     8581 atcgtctgtt tgtggatgta cagagtgata ttattgacac tcccgggcga cggatggtga
     8641 tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg
     8701 tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggttt
     8761 ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca
     8821 ttaacctgat gttctgggga atataaggtc tccatctacc atagtagaaa ttagacgagc
     8881 ttactcgttt cgtcctcacg gactcatcag taattttttt tttttttttt tttttttttt
     8941 tttttttttt tttttttttt tttttttttt tttttttttt ttcttttttt tttttttttt
     9001 tttttcactt ctttttctta gcctgtccgg cctttttggt ggcagcagga cgcttgcggt
     9061 tctgcgcgaa attcagccat gtctgattgt caatggcgag cttcaccttg ttcagatcgt
     9121 ctgagttctt gagctcattc aggagccaca ggcccttgag ggcaatgtgg tatgcgccgt
     9181 tggcatccgc attctgtggc tgtgtatcat cggccagtgc tgaattgaag aagacgccct
     9241 catcgttggc cacaggagag aggatgaaat cctcgtcgct ggaggcgttt gagtacctga
     9301 gggccaggag tgtcttcagg aggtacatca gagacttatg gaactccttg tcattgttct
     9361 ggcagatatc catgacgaga tttggctgct tgtcgttaat gtggtaccgg gtgaacagtg
     9421 acttgagctc atcattgacg ttcacgccga ttgtcgcgcc cttattctgg ttggcggtct
     9481 tgtcgtacac gtagcgctta tcgccatggc tgcaaatctt ccagatctgc ctggaattct
     9541 tcgccttgtc gttgaacttg gcgtaatcaa tgtggaactc gaagtagccc cggtccgcat
     9601 tgtagcagat cttatcgaac ttgccgaaga acgcctgaga ctgggcaatg ttctcgtagc
     9661 gaggcttcag gaggtccacg aagcctgtct ccggatcaat ctttgaggtg ttccaggcag
     9721 gcacgtagaa gaggaagccg gtctgcttgc cgatagactt caggtctgtg aaattgttgg
     9781 tcagctggag cgcattcttg tatgagccga tctcatcgtc ggccttatcc ttcaggacga
     9841 ggtggttgag cttcttaatc agcgcattct cgaagttctg gtagatctgc ttctccacct
     9901 tgaacctgcc ccgcttgaag ccgaaattca ggtcctcgag gaccacaatg gcgttgtact
     9961 tcagcatgag ctggctgatc tgatggacca cgtgagagag gtagcctgac ttcagctcct
    10021 tgattgtctc gatctcgccc catcccaccc gggcattgag cctctcaatc tccctcttgt
    10081 ccaggatctt atggtatggt gtggtcatct gtgtgccatt cgcagaggct gtggtgatat
    10141 cgttgagtga cctctgctcc agaatctcgc ccttagaatt gatgacggtg aggtacagga
    10201 ggtgcctctc gccgcgatca attccaatga cgttcacctc atcgtactgc tggattgact
    10261 ggttcacctt cttattgaac tccttaattg tcatgccctg gacgccgaaa ttcatggtga
    10321 tcggcacatg gagcatgaac ttatcctgtg tgtaccgctt atccttaatg atgtcgtaga
    10381 cgaactggcg cttcttcggg ttgtcaggat tcttgttctc gagcacctcg ccggccctgt
    10441 gaattgttgt ctcgttcata tccagggacg ccttccggta gaaaatctcg gcctcgccgt
    10501 tgagcttgta gataggattc acgaggttgt cctcgctgaa cagcgccttg aagtacaggg
    10561 tatggagatt aggcttgccg tgggcctttg gggagaaatc cttgttgtag atctggaaca
    10621 ggtagagctg gccctgctcg acgagctcat taatgtaatc ggcgttgatg tccacgaact
    10681 tcacctggta gccctgtggc tccacctcgc ggtagaaatc gctgaggtcc tggtaagatg
    10741 atgttgggga gaacttgaag ccgaaatgct gccactccgg gtgcttatta atgcccgcct
    10801 tgaagaagtc gatgagggca tggcaatcct tcagattgaa gttgtcgccc ttcttgtggg
    10861 tgccctgcgc gtacttgtcc aggagctcgg cgctaggatt gtagtaatcg aggttggact
    10921 tcgcgaagaa cacctttggc agcatcttgt ttgggcccgg caggagcttg tagatcatct
    10981 tctggtagac gctcttgcct gtattcgggg cgttgtcgaa caccttcttg tgcgccttat
    11041 ccaggagggc gaggtagtag cagccgtcct tctgcaggat gacgccgaaa ttatccttct
    11101 ccttgttcag gtcccagcca ttcaggagtg tcggattgcc gaagttgagc ttgtacttct
    11161 cggtagagaa aggcttctgt gagaggtaat cgcgcacctt gttgtacagg gtcgcgatct
    11221 tggcgagctc gtcgtacagc gcgccgaact cgccgtagaa gttgccatcc tgattatgga
    11281 gtgtggtctt tgtggtcagg agcttcgcga agtgggcgac attgagggcg ttgtccagga
    11341 gttccttcag ctgccgaatc tctggagact tatcggactt gatctttggc agggccctct
    11401 cgccggccgg gcgctcccgc tcgaggaagc ccttgattgt gctatgattg ttgtgaatct
    11461 tctggattgg gttgtccacg ccggcgaggc catgcttgaa gtactgtccc agcttgccgg
    11521 cctgcactga ctcatcgtca tgcctggcgg tgtagtgctc aatggcctgc tccaggctgg
    11581 ccagtgagtg cactccctta atgaacttgt ccttctcctt tgtgagcttc gccttggcgt
    11641 tatcggtctt cgccttggcg aagcgctcgt tgaactccgg attgaccaca tcgacgtagt
    11701 atccatccag caccctgccc aggagggcga aatcgccgaa ggcctgcttg ctcagctcat
    11761 tgaggttctt gtactcgacg tagatgccgt ccttctggta atcgtcgaag ccatcgaaga
    11821 gggactggac cttcgcgaac acgtcggcgt aatgccggta gaactcgttg accgcctggc
    11881 acacctcgct atcgtcggcg aacttgcttg ggaggaagga cacgcccatg ccatcggaca
    11941 gaatctgctt gtgcagcggg cggagcttgg cgatcctctc agacttatgg cagtgctggt
    12001 tatggtgtga attgatgagc tcgttaatgc cctggatctt gcggctgccg gcctctcctg
    12061 aaatgccgcc caggagtgta ttgtaggcgg tgatgccctc ctgtgtcagg agcttatggt
    12121 agccgtcgag gtgagacgcc agtgagacat cgaggccgct ggctgtgagc tcgttaatga
    12181 tctggtcgta cagcgcggaa tgcttctgct taatggtggc caggatctgg agattatcaa
    12241 tgaacctcgg gaggttctca tgaatcagcc ggtacgcgat ggcggtgtgc ttatcctcgt
    12301 cagagtacat attcttcctg ttgtcgtgga agcctgtgaa gtaggttgag aacttctcga
    12361 aatgcgccag gtgggcgagc ttagggctgg actctccctc ctgggcaatc acgaacttgg
    12421 ccagatcgcc gagctccttg ccgtccttga agagcttcgc gccgaacagc ctatcgtagc
    12481 cggccttgta cttgccgcca ttgccaatag gcttgacgat ctccttccgc agcacggcct
    12541 ggaggtcctt gagctgcttc tggaggccat cgtcctttgg gttcttgcgg aacttcaggt
    12601 agacatcgta gaactccgcc agcttggtga gcttcacctc gcccatcata tcggcaatga
    12661 agtccctatg gtaatcgtcc aggatcgcct tgaccttctg gtacatatcg gccattgtct
    12721 cgtcctgatt gaggaagttc ttggcatgaa tgtgctcgag ggtcttgccg ataggcttca
    12781 gctcgaaccg cactgtcttg gacagtgggt agaggtgggt gaagtcctgg aagagagccg
    12841 caggcacccc gtgaatacca accttccgct tcttcttagg agccatggcg gcctgcagga
    12901 agtaacacca aacaacaggg tgagcatcga caaaagaaac agtaccaagc aaataaatag
    12961 cgtatgaagg cagggctaaa aaaatccaca tatagctgct gcatatgcca tcatccaagt
    13021 atatcaagat caaaataatt ataaaacata cttgtttatt ataatagata ggtactcaag
    13081 gttagagcat atgaatagat gctgcatatg ccatcatgta tatgcatcag taaaacccac
    13141 atcaacatgt atacctatcc tagatcgata tttccatcca tcttaaactc gtaactatga
    13201 agatgtatga cacacacata cagttccaaa attaataaat acaccaggta gtttgaaaca
    13261 gtattctact ccgatcttga acgaatgaac gaccgcccaa ccacaccaca tcatcacaac
    13321 caagcgaaca aaaagcatct ctgtatatgc atcagtaaaa cccgcatcaa catgtatacc
    13381 tatcctagat cgatatttcc atccatcatc ttcaattcgt aactatgaat atgtatggca
    13441 cacacataca gatccaaaat taataaatcc accaggtagt ttgaaacaga attaattcta
    13501 ctccgatctt gaacgaccgc ccaaccagac cacatcatca caaccaagac aaaaaaaagc
    13561 atgaaaagat gacccgacaa acaagtgcac ggcatatatt gaaataaagg aaaagggcaa
    13621 accaaaccct atgcaacgaa acaaaaaaaa tcatgaaatc gatcccgtct gcggaacggc
    13681 tagagccatc ccaggattcc ccaaagagaa acactggcaa gttagcaatc agaacgtgtc
    13741 tgacgtacag gtcgcatccg tgtacgaacg ctagtagcac ggatctaaca caaacacgga
    13801 tctaacacaa acatgaacag aagtagaact accgggccct aaccatggac cggaacgccg
    13861 atcttgagaa ggtagagagg gggggggggg aggacgagcg gcgtaccttg aagcggaggt
    13921 gccgacgggt ggatttgggg gagttctggt tgtgtgtgtg tgcgctccga acaacacgag
    13981 gttggggaaa gagggtgtgg agggggtgtc tatttattac ggcgggcgag gaagggaaag
    14041 cgaaggagcg gtgggaaagg aatcccccgt agctgccggt gccgtgagag gaggaggagg
    14101 ccgcctgccg tgccggctca cgtctgccgc tccgccacgc aatttctgga tgccgacagc
    14161 ggagcaagtc caacggtgga gcggaactct cgagaggggt ccagaggcag cgacagagat
    14221 gccgtgccgt ctgcttcgct tggcccgacg cgacgctgct ggttcgctgg ttggtgtccg
    14281 ttagactcgt cgacggcgtt taacaggctg gcattatcta ctcgaaacaa gaaaaatgtt
    14341 tccttagttt ttttaatttc ttaaagggta tttgtttaat ttttagtcac tttattttat
    14401 tctattttat atctaaatta ttaaataaaa aaactaaaat agagttttag ttttcttaat
    14461 ttagaggcta aaatagaata aaatagatgt actaaaaaaa ttagtctata aaaaccatta
    14521 accctaaacc ctaaatggat gtactaataa aatggatgaa gtattatata ggtgaagcta
    14581 tttgcaaaaa aaaaggagaa cacatgcaca ctaaaaagat aaaactgtag agtcctgttg
    14641 tcaaaatact caattgtcct ttagaccatg tctaactgtt catttatatg attctctaaa
    14701 acactgatat tattgtagta ctatagatta tattattcgt agagtaaagt ttaaatatat
    14761 gtataaagat agataaactg cacttcaaac aagtgtgaca aaaaaaatat gtggtaattt
    14821 tttataactt agacatgcaa tgctcacctt atctcaagag aggggcacga ccgggtcacg
    14881 ctgcactgca ggcgcgccca tggtggagca cgacactctc gtctactcca agaatatcaa
    14941 agatacagtc tcagaagacc aaagggctat tgagactttt caacaaaggg taatatcggg
    15001 aaacctcctc ggattccatt gcccagctat ctgtcacttc atcaaaagga cagtagaaaa
    15061 ggaaggtggc acctacaaat gccatcattg cgataaagga aaggctatcg ttcaagatgc
    15121 ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga
    15181 agacgttcca accacgtctt caaagcaagt ggattgatgt gaacatggtg gagcacgaca
    15241 ctctcgtcta ctccaagaat atcaaagata cagtctcaga agaccaaagg gctattgaga
    15301 cttttcaaca aagggtaata tcgggaaacc tcctcggatt ccattgccca
//
