LOCUS       sequence-45628-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Banko MR, Allen JJ, Schaffer BE, Wilker EW, Tsou P, White JL, Villen
            J, Wang B, Kim SR, Sakamoto K, Gygi SP, Cantley LC, Yaffe MB, Shokat
            KM, Brunet A
  TITLE     Chemical Genetic Screen for AMPKalpha2 Substrates Uncovers a Network
            of Proteins Involved in Mitosis.
  JOURNAL   Mol Cell. 2011 Dec 23;44(6):878-92. Epub 2011 Dec 1.
  PUBMED    22137581
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2011-12-23"; volume: "44(6)"; pages: "878-92. Epub 2011 Dec 
            1"
COMMENT     Sequence Label: pECE-M2-CDC27 S379A
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             56..79
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agaggctttt ttggaggaga tctaagcttg gtaccgagct cggatcctca ggatggacta
       61 caaggacgat gacgataaac ccgggaattc gacggtgctg caggaacccg tccaggctgc
      121 tatatggcaa gcactaaacc actatgctta ccgagatgcg gttttcctcg cagaacgcct
      181 ttatgcagaa gtacactcag aagaagcctt gtttttactg gcaacctgtt attaccgctc
      241 aggaaaggca tataaagcat atagactctt gaaaggacac agttgtacta caccgcaatg
      301 caaatacctg cttgcaaaat gttgtgttga tctcagcaag cttgcagaag gggaacaaat
      361 cttatctggt ggagtgttta ataagcagaa aagccatgat gatattgtta ctgagtttgg
      421 tgattcagct tgctttactc tttcattgtt gggacatgta tattgcaaga cagatcggct
      481 tgccaaagga tcagaatgtt accaaaagag ccttagttta aatcctttcc tctggtctcc
      541 ctttgaatca ttatgtgaaa taggtgaaaa gccagatcct gaccaaacat ttaaattcac
      601 atctttacag aactttagca actgtctgcc caactcttgc acaacacaag tacctaatca
      661 tagtttatct cacagacagc ctgagacagt tcttacggaa acaccccagg acacaattga
      721 attaaacaga ttgaatttag aatcttccaa ttcaaagtac tccttgaata cagattcctc
      781 agtgtcttat attgattcag ctgtaatttc acctgatact gtcccactgg gaacaggaac
      841 ttccata
//
