LOCUS       sequence-45630-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Banko MR, Allen JJ, Schaffer BE, Wilker EW, Tsou P, White JL, Villen
            J, Wang B, Kim SR, Sakamoto K, Gygi SP, Cantley LC, Yaffe MB, Shokat
            KM, Brunet A
  TITLE     Chemical Genetic Screen for AMPKalpha2 Substrates Uncovers a Network
            of Proteins Involved in Mitosis.
  JOURNAL   Mol Cell. 2011 Dec 23;44(6):878-92. Epub 2011 Dec 1.
  PUBMED    22137581
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2011-12-23"; volume: "44(6)"; pages: "878-92. Epub 2011 Dec 
            1"
COMMENT     Sequence Label: pECE-M2-GPHN
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             56..79
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agaggctttt ttggaggaga tctaagcttg gtaccgagct cggatcctca ggatggacta
       61 caaggacgat gacgataaac ccgggaattc ggcgaccgag ggaatgatcc ttactaacca
      121 cgaccatcaa atccgtgtcg gagtccttac agtgagtgat agttgcttca ggaatcttgc
      181 agaagaccgc agtgggataa atctcaaaga tctcgtacaa gatccttctt tgttgggtgg
      241 gactatatca gcatacaaga tagtaccaga tgaaatagaa gaaatcaagg aaaccctgat
      301 agattggtgt gatgaaaagg aacttaattt gatattaaca actggaggaa caggatttgc
      361 accacgagat gtcactccag aggccacaaa agaagtaata gaacgggaag caccagggat
      421 ggccctggca atgctgatgg gatcacttaa tgttacacct ctgggcatgc tctctaggcc
      481 tgtatgtgga atcagaggga aaacgcttat aattaacctg ccaggtagca agaaaggatc
      541 tcaggaatgc tttcaattca tactgccagc tctacctcat gccattgacc ttttacgtga
      601 tgccattgta aaagtaaagg aggtgcatga tgaacttgaa gatttgcctt ccccacctcc
      661 ccctctttcc cctcctccta ctaccagccc ccataaacag acagaagaca aaggagttca
      721 atgtgaggaa gaggaagaag agaagaaaga cagtggtgtt gcttcaacag aagatagttc
      781 ctcatcacat ataactgcag cagccattgc tgccaagaag catccattct acaccagtcc
      841 tgctgttgtc atggcacacg gtgaacagcc catccctggt ctcatcaatt
//
