LOCUS       sequence-456313-        2387 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N terminal mTurquoise2 fluorescence tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Lai Z, Flanigan SF, Boudes M, Davidovich C
  TITLE     Modular cloning of multigene vectors for the baculovirus system and 
            yeast.
  JOURNAL   J Mol Biol. 2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943.
  PUBMED    39814169
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943."
COMMENT     Sequence Label: pM.005
FEATURES             Location/Qualifiers
     source          1..2387
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(14..602)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(94..113)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             784..1443
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(850..869)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1514..1548
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
       61 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
      121 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
      181 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
      241 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
      301 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
      361 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
      421 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
      481 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
      541 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
      601 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
      661 aagggatttt ggtcatgatt gatcgggcac gtaagaggtt ccaactttca ccataatgaa
      721 ataagatcac taccgggcgt attttttgag ttatcgagat tttcaggagc taaggaagct
      781 aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa
      841 gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg
      901 gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt
      961 attcacattc ttgcccgcct gatgaatgct catccggaat ttcgtatggc aatgaaagac
     1021 ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact
     1081 gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata
     1141 tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt
     1201 gagaatatgt ttttcgtttc agccaatccc tgggtgagtt tcaccagttt tgatttaaac
     1261 gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa
     1321 ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc
     1381 catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg
     1441 taatttgata tcgagctcgc ttggactcct gttgatagat ccagtaatga cctcagaact
     1501 ccatctggat ttgttcagaa cgctcggttg ccgccgggcg ttttttattg gtctggtctc
     1561 acataccgga tttgtacaat tcatccatac ccaaggtaat accagcagca gtaacaaatt
     1621 ctaacaagac catgtggtcc ctcttttcgt ttggatcttt ggataactta gattgagtgg
     1681 ataagtaatg gttgtctggt aacaagactg gaccatcacc aattggagta ttttgttgat
     1741 aatggtcagc taattgaaca ccaccatctt caatgttgtg tctaattttg aagttagctt
     1801 tgataccatt cttttgtttg tcagcagtga tgtaaacatt gtcagagaaa taattgtatt
     1861 ccaatttgtg acctaaaatg ttaccatctt ctttaaaatc aatacctttt aattcgattc
     1921 tattaactaa ggtatcacct tcaaacttga cttcagctct ggtcttgtag ttaccgtcat
     1981 ctttgaaaaa aatagttctt tcttgaacat aaccttctgg catggcagac ttgaaaaagt
     2041 catgttgttt catatgatct gggtatcttg caaaacattg aacaccccaa gataaagtag
     2101 tgactaaggt tggccatgga actggcaatt taccagtagt acaaataaat tttaaggtca
     2161 atttaccgta agtagcatca ccttcacctt caccggagac agaaaatttg tgaccattaa
     2221 catcaccatc taattcaacc aaaattggga caacaccagt gaataattct tcacctttag
     2281 aaaccatctt tgagaccgac tacggttatc cacagaatca ggggataacg caggaaagaa
     2341 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccg
//
