LOCUS       sequence-456316-        2381 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N terminal mRuby2 fluorescence tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Lai Z, Flanigan SF, Boudes M, Davidovich C
  TITLE     Modular cloning of multigene vectors for the baculovirus system and 
            yeast.
  JOURNAL   J Mol Biol. 2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943.
  PUBMED    39814169
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943."
COMMENT     Sequence Label: pM.007
FEATURES             Location/Qualifiers
     source          1..2381
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             13..723
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     terminator      complement(746..780)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(851..1510)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1425..1444
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1692..2280
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2181..2200
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa agatggtgtc caaaggagag gagttaatca aggaaaacat gagaatgaaa
       61 gttgtcatgg agggctccgt taatggtcac caattcaagt gtacagggga aggtgaaggt
      121 aatccttaca tgggtacaca aactatgaga attaaagtaa ttgaaggcgg accactacca
      181 tttgcatttg acattctggc aacgtcattc atgtacggat cacgaacttt catcaagtac
      241 cctaaaggta taccagactt tttcaagcaa tcttttccag agggttttac atgggaaagg
      301 gttacaagat acgaagatgg gggtgtcgtc acagttatgc aagatacttc attagaagat
      361 ggctgccttg tctatcatgt gcaagtaaga ggggtgaatt ttccttctaa cggacctgtg
      421 atgcagaaaa agaccaaagg ttgggaacca aatactgaaa tgatgtaccc agctgatgga
      481 ggtttgagag gctacacaca catggcgctt aaagttgatg gtggaggtca tttgtcttgt
      541 agttttgtta ccacttatcg ttctaaaaag actgttggca atatcaaaat gccaggaata
      601 catgctgtag accacagact agaaagactc gaagagagcg ataacgaaat gttcgttgta
      661 cagagagagc atgccgtagc caaatttgct ggcttaggcg gtggtatgga tgaattgtat
      721 aagtccggta tgtgagacca gaccaataaa aaacgcccgg cggcaaccga gcgttctgaa
      781 caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct
      841 cgatatcaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca
      901 ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca
      961 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt
     1021 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgaaacga
     1081 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca
     1141 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg
     1201 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata
     1261 tcaccagctc accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg
     1321 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa
     1381 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg
     1441 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt
     1501 ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg
     1561 gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaatcatg
     1621 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     1681 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     1741 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     1801 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     1861 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     1921 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     1981 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     2041 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     2101 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     2161 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     2221 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     2281 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     2341 ttctttcctg cgttatcccc tgattctgtg gataaccgta g
//
