LOCUS       sequence-456317-        1766 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N terminal 3xFlag-6xHis affinity tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Lai Z, Flanigan SF, Boudes M, Davidovich C
  TITLE     Modular cloning of multigene vectors for the baculovirus system and 
            yeast.
  JOURNAL   J Mol Biol. 2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943.
  PUBMED    39814169
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943."
COMMENT     Sequence Label: pM.008
FEATURES             Location/Qualifiers
     source          1..1766
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             16..87
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     CDS             91..108
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      complement(131..165)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(236..895)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     810..829
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1077..1665
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1566..1585
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa agatggatta caaggacgat gatgataagg actacaaaga tgatgatgac
       61 aaagattata aggacgatga tgataaaggt catcatcatc atcatcactc cggtatgtga
      121 gaccagacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
      181 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg
      241 ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga
      301 agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
      361 gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt
      421 ttaaatcaaa actggtgaaa ctcacccagg gattggctga aacgaaaaac atattctcaa
      481 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata
      541 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag
      601 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
      661 ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa
      721 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
      781 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
      841 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
      901 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
      961 cattatggtg aaagttggaa cctcttacgt gcccgatcaa tcatgaccaa aatcccttaa
     1021 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1081 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1141 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1201 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     1261 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1321 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1381 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1441 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1501 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1561 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     1621 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     1681 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     1741 tcccctgatt ctgtggataa ccgtag
//
