LOCUS       sequence-456318-        1818 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N terminal Strep affinity tag with TEV cleavage site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Lai Z, Flanigan SF, Boudes M, Davidovich C
  TITLE     Modular cloning of multigene vectors for the baculovirus system and 
            yeast.
  JOURNAL   J Mol Biol. 2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943.
  PUBMED    39814169
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943."
COMMENT     Sequence Label: pM.009
FEATURES             Location/Qualifiers
     source          1..1818
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             17..40
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             77..100
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             140..160
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     terminator      complement(183..217)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(288..947)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     862..881
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1129..1717
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1618..1637
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa agaatgtggt cgcatccaca atttgagaaa ggtggaggtt ccggaggtgg
       61 atcgggaggt ggatcgtgga gccacccgca gttcgaaaaa gagctcaaaa ccgcggctct
      121 tgcgcaacac gatgaagccg agaatttgta ttttcagggt tccggtatgt gagaccagac
      181 caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg
      241 tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc
      301 tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca
      361 caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa
      421 tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca
      481 aaactggtga aactcaccca gggattggct gaaacgaaaa acatattctc aataaaccct
      541 ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga
      601 aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca
      661 tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt
      721 gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga
      781 taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg
      841 gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc
      901 cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta
      961 gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg
     1021 tgaaagttgg aacctcttac gtgcccgatc aatcatgacc aaaatccctt aacgtgagtt
     1081 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     1141 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     1201 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     1261 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     1321 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     1381 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     1441 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     1501 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     1561 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     1621 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     1681 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     1741 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     1801 ttctgtggat aaccgtag
//
