LOCUS       sequence-456326-        1825 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C terminal Strep affinity tag with TEV cleavage site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Lai Z, Flanigan SF, Boudes M, Davidovich C
  TITLE     Modular cloning of multigene vectors for the baculovirus system and 
            yeast.
  JOURNAL   J Mol Biol. 2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943.
  PUBMED    39814169
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943."
COMMENT     Sequence Label: pM.010
FEATURES             Location/Qualifiers
     source          1..1825
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             20..40
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             80..103
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             140..163
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     terminator      complement(190..224)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(295..954)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     869..888
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1136..1724
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1625..1644
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa tcctcaggtg agaatttgta ttttcagggt gagctcaaaa ccgcggctct
       61 tgcgcaacac gatgaagcct ggtcgcatcc acaatttgag aaaggtggag gttccggagg
      121 tggatcggga ggtggatcgt ggagccaccc gcagttcgaa aaataactcg agtggctgag
      181 accagaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt
      241 tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc
      301 cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa
      361 gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg
      421 cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt
      481 taaatcaaaa ctggtgaaac tcacccaggg attggctgaa acgaaaaaca tattctcaat
      541 aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat
      601 gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt
      661 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc
      721 tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa
      781 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag
      841 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt
      901 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
      961 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc
     1021 attatggtga aagttggaac ctcttacgtg cccgatcaat catgaccaaa atcccttaac
     1081 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     1141 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     1201 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     1261 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     1321 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     1381 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     1441 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     1501 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     1561 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     1621 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     1681 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     1741 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     1801 cccctgattc tgtggataac cgtag
//
