LOCUS       sequence-456368-        1542 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses zebrafish dgat1a with a C-terminal FLAG tag under control 
            of the CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Wilson MH, Hensley MR, Shen MC, Lu HY, Quinlivan VH, Busch-Nentwich 
            EM, Rawls JF, Farber SA
  TITLE     Zebrafish are resilient to the loss of major diacylglycerol 
            acyltransferase enzymes.
  JOURNAL   J Biol Chem. 2024 Nov 6;300(12):107973. doi: 
            10.1016/j.jbc.2024.107973.
  PUBMED    39510175
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2024-11-6"; volume: "300(12):107973. doi"; pages: " 
            10.1016/j.jbc.2024.107973"
COMMENT     Sequence Label: pcDNA3.1+ dgat1a-FLAG
FEATURES             Location/Qualifiers
     source          1..1542
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1510..1533
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ggtaccgcaa acatgggcga cagaaacgag aagggctcgg cgaagcaccg gcggaggact
       61 acgatctccg gagaggcggc ggtgactcaa gccaaacgcg gcggcgcagc agagacgctg
      121 agcgggcagg tgaaggagaa agagccgcag aaacacgaga atgcagccgg tagacagaag
      181 aaccacagcg atgctggaga ggacacattc agctgtcaca agctgcagga gtcactgctc
      241 agttcggcca gtggttttag caactacaga ggaatcctca actggtgtgt ggtcatgctg
      301 gttttgtcta acgcacggct cgtgctggag aaccttataa aatatggcgt tttggtggac
      361 cccatccaga tcatctctct gttcgtgaag gacccctgca gctggccggc tctgtgcctg
      421 atcatcgtca ctaacgtctt cataatggcg gctctgtaca ccgagagaaa gctgtctgtg
      481 ggcaccatct ctgagagaac cggatctttc cttcactgca tcaatctgtc tgcactgttg
      541 tttgtgccgg caggaacggt gctaagcctg agctccgtca caccagtggg cggtgtgatg
      601 gcactgagta tctgcactgt tctcttcctc aagctttact cgtacacaga tgttaataag
      661 tggtgccgag aacagagaca agccaaagct cgaaccctgt ctcgctctca ctcctgtccg
      721 tccgtgcaca aagcaaatgg cacagcggga tacacacacg tgacgtatcc gggaaacctc
      781 acacacagag acatttatta ttttgctttt gcaccgactc tgtgttacga gctgaatttt
      841 ccgcgctcac cacgaatacg caaacgtttc ctactgcggc gactgctgga gatgctgttt
      901 ctaatgcaac tgatggtcgg attaatacag cagtggatgg ttccgacgat tcagaactct
      961 atgaaaccct ttcaggacat ggatttctcc agaatggtgg agcggatgct gaaactcgct
     1021 gtgcccaatc actttatctg gctcatcttt ttctattggt atttccactc ctctatgaac
     1081 ttcgtagcag agatcatgca gttcggggac cgcgagtttt accgcgactg gtggaactct
     1141 gaaacgattc cttatttctg gtcaaactgg aacattcctg ttcacaagtg gtgcctacga
     1201 cacttttata agcccatgtt gatgcgagga gcaaatcgat tagcagctca gatcgccgtc
     1261 ttcttcctct ccgctttttt ccacgagtat ttagtgagtg ttcctctcaa gatgtttcgg
     1321 ctctgggcgt ttctgggtat gatgctgcag gtcccgctgg ccattttggt tggccgatac
     1381 ctgaggggta actatggcaa cgccgccgtg tggatgtctc tgatcatcgg ccagccaatc
     1441 gcagtgctca tgtacgtcca cgactactac gtgttgcatt atggcagcgc gtctgaggcc
     1501 gcagcgccgg actataaaga cgatgatgac aaatgaggta cc
//
