LOCUS       sequence-456369-        1566 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses zebrafish dgat1b with a C-terminal FLAG tag under control 
            of the CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Wilson MH, Hensley MR, Shen MC, Lu HY, Quinlivan VH, Busch-Nentwich 
            EM, Rawls JF, Farber SA
  TITLE     Zebrafish are resilient to the loss of major diacylglycerol 
            acyltransferase enzymes.
  JOURNAL   J Biol Chem. 2024 Nov 6;300(12):107973. doi: 
            10.1016/j.jbc.2024.107973.
  PUBMED    39510175
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2024-11-6"; volume: "300(12):107973. doi"; pages: " 
            10.1016/j.jbc.2024.107973"
COMMENT     Sequence Label: pcDNA3.1+ dgat1b-FLAG
FEATURES             Location/Qualifiers
     source          1..1566
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1534..1557
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gatatcgcaa acatgattga caaggatgat ttcgggcccg tcacccgaaa tcggcggcgc
       61 gcgaccatca cgagtaccag gagcgagtct gtgaagctgc gaaacggcgc gagcgcgaca
      121 gcagcagccg ctgacagtgg tctcaaaagc ggacacaagg cgcgggagtc tctggcgaaa
      181 aacttgcagc tcaacgaaga aaacaagaga aaatgcgacc gctacgacag aatgagttgc
      241 cacaagttgc aggagtcgat gttgagttca gcgagcagct tcaaaaacta cagagggatc
      301 ctgaactggt gtgtggtaat gctggttttg agcaacgcac gtctcttttt agaaaacctt
      361 ctcaggtatg gcattttagt ggaccccatt caggtggtgt ctttgtttct caaggaccct
      421 tatagctggc ctgctgcatg tctggttatt gtttgtaatg cttttatact tgtggcactg
      481 tacaccgaga gaaagctagc catggggtcg atcagtgaaa aggtcggcct gctgatctac
      541 atctttaatt taacaattat cttgtgtttt ccaatggttg ttgttttaaa actaccatct
      601 attacgccag ttggaggggc atttgcctta ggaatctaca ccatcctgtt tttgaaactg
      661 tattcataca aagacgttaa caagtggtgc agagagagaa ctcaagcaaa ggcccgcagc
      721 ctctctagat ctctttcatg tccatcacct ccatctacat ccagcagtat gcagtcttat
      781 gtgtcttacc caggaaacct ctctctcagg gatatatact attttgtttt tgctccgacc
      841 ctgtgctacg agcttaattt cccccgatct gaatcaatcc gaatgggatt tctcctcagg
      901 aggctctttg agatgttgct tctcactcag ttattggttg gtttaacgca gcagtggatg
      961 gtgccaatca ttcggagttc aatgaagcca ttgcaggaaa tggactacac tagaatgaca
     1021 gagcggctgc ttaggctggc agtgcccaat catctcctct ggctaatatt cttctactcg
     1081 tttttctatt cctcaatgaa cttcatggct gagctgttga ggtttggaga tcgggaattt
     1141 taccgtgact ggtggaattc tgaaacgata acgtacttct ggcaaaactg gaatattccc
     1201 gtgcacaagt ggtgcctccg gcacttttac aagccgctgc tgagacgagg tgccggcaag
     1261 ttactgagcc aatcagctgt gtttttcgcc tctgcttttt ttcatgagta tctggtcagt
     1321 gttcctctca ggatgttcag actgtgggca tttatgggca tgatggcaca gattccactg
     1381 gcatggttag tgtccaggtt tcttcgaggc aactatggaa atgctgctgt gtggctctca
     1441 ctcattatcg gtcaacctat cgccgttctc atgtatgttc atgactacta tgtcacaaat
     1501 caccaagacg acctgacaat caatgaagat ctggactata aagacgatga tgacaaatga
     1561 actagt
//
