LOCUS       sequence-456374-        8418 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mouse EHMT1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8418)
  AUTHORS   Biligiri KK, Sharma NR, Mohanty A, Sarkar DP, Vemula PK, Rampalli S.
  TITLE     A cytoplasmic form of EHMT1N methylates viral proteins to enable 
            inclusion body maturation and efficient viral replication
  JOURNAL   PLOS Biology 22(11): e3002871.
REFERENCE   2  (bases 1 to 8418)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLOS 
            Biology 22(11): e3002871."
COMMENT     Sequence Label: mCherry_EHMT1_full length
FEATURES             Location/Qualifiers
     source          1..8418
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    2555..2676
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2592..2611)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2646..2665
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2683..3138)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2820..2841)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3032..3051
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3165..3269
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3271..3628
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3271..3462
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3294..3314)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3479..3614
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3663..4457
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3663..4457
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3717..3736)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4327..4346
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4645..4664)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4689..4736
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5065..5653
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5554..5573
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        5828..6131
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6132..6335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6286..6306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      6374..6383
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6380..7087
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6525..6543)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6793..6812
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tagaaagaag ccgagcagca tgcttggttc cgaggcttgt aagtcatctc caggaagcat
       61 ggagcaggca gctctgggag acagcgctgg ctatatggaa gtttccctgg attccctgga
      121 tctccgtgtc agaggaattc tgtcttccca gacagaaaat gaagggctgg ccagtggtcc
      181 ggatgtgctg gggacggatg gcctccagga agtgcctctc tgcagctgcc gaatggaaac
      241 ccccaagagc cgcgagatca gcaccctggc caacaaccag tgcatggcca ctgagagcgt
      301 ggatcacgaa ggtaacttca tggaatgcca gcctgagagc agcatctctc atcgtttcca
      361 taaggactgt gcctctcgag tcaacaatgc cagctactgt ccccattgtg gggaagaagc
      421 ttccaaggcc aaagaggtga ccatagcaaa agcagacaca acttccacag tgaccctagc
      481 ccctggacag gagaagagcc tggctgctga aggcagggct gacacgacca cgggcagcat
      541 tgctggagcc ccagaggatg aaagatcgca gagcacagcc ccccaggcac cagagtgctt
      601 cgaccctgcc ggaccggctg ggctcgtgag gccgacatct ggcctttccc agggcccagg
      661 aaaggaaacc ttggaaagtg ctctaatcgc tctagactct gaaaaaccca agaaacttcg
      721 cttccaccca aagcagctgt acttctctgc caggcagggt gagctgcaga aggtgcttct
      781 catgctggtt gatggaattg atcccaactt caaaatggag caccaaagta agcgttcccc
      841 attacatgct gctgcggagg ctggccacgt ggacatctgc cacatgctgg ttcaggcggg
      901 tgccaatatt gacacttgct cagaggacca gcggacccca ctgatggagg ctgcagagaa
      961 caaccacttg gatgcagtga agtacctcat caaggctgga gcacaggtgg atccgaagga
     1021 cgcagagggc tccacatgtt tgcatttggc tgccaagaaa ggccactatg atgtggttca
     1081 gtatctgctt tcaaatggac agatggatgt caactgccag gatgacggtg gatggacacc
     1141 tatgatctgg gccactgagt acaagcacgt ggagctggtg aagctgctgc tgtctaaggg
     1201 ctctgacatc aacatccggg acaacgagga gaacatttgt ctgcactggg cagcattttc
     1261 aggctgtgtg gacatagctg aaatacttct ggctgccaag tgtgacctgc atgctgtgaa
     1321 tatccatgga gactcacccc tgcacatcgc agccagggag aatcgctacg actgtgttgt
     1381 cctctttctt tctcgggatt cagatgttac tctgaaaaac aaggaaggag agactccctt
     1441 gcagtgtgca agtctcagtt cgcaggtgtg gagtgcattg cagatgagca aagcacttcg
     1501 ggactcagcc cctgacaagc ccgttgctgt tgagaagacg gtgagcaggg atatcgctcg
     1561 agggtatgag cgcattccca ttccctgtgt caatgctgtg gacagtgagc tgtgtcctac
     1621 caactataag tatgtctccc agaactgtgt gacatccccc atgaacattg acaggaacat
     1681 cactcatttg cagtactgcg tgtgtgtaga tgactgctcc tctagcacct gcatgtgtgg
     1741 ccagctgagc atgcgctgct ggtatgataa ggatggccga cttctgcctg agtttaacat
     1801 ggcagaacca cccttgatct tcgagtgcaa tcatgcctgc tcatgctgga ggaactgccg
     1861 caatcgtgtg gtgcaaaatg gtctcagggc aaggctgcag ctttatcgga cacaggacat
     1921 gggctggggt gtgcggtccc tccaggatat cccactgggc acctttgtct gcgaatacgt
     1981 aggggagctg atttcggact ctgaagctga tgttcgggaa gaggactctt acctctttga
     2041 tcttgacaat aaggatggag aggtatactg cattgacgct cggttctatg ggaatgtcag
     2101 ccggttcata aaccaccact gcgaacccaa ccttgtgcct gtgcgagtgt tcatgtcaca
     2161 ccaggacctg cggtttccca ggattgcctt cttcagtacc cgcctgattc aggctgggga
     2221 gcagctcggg ttcgactacg gggagcgctt ttgggacgtc aagggcaagc tcttcagttg
     2281 ccggtgtggg tcttccaagt gtcggcactc aagcgcagcc ctggcccaga ggcaagccag
     2341 tgcagcccag gagcctcagg agaatggcct tccagatacc agctctgcag ccgctgctga
     2401 ccccctatga ggtaccgcgg gcccgggatc caccggatct agataactga tcataatcag
     2461 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     2521 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     2581 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     2641 tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta agcgttaata
     2701 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     2761 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     2821 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     2881 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     2941 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     3001 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     3061 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     3121 cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     3181 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     3241 tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt
     3301 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3361 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     3421 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     3481 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     3541 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     3601 cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc
     3661 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     3721 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     3781 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     3841 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     3901 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     3961 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     4021 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     4081 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     4141 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg
     4201 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     4261 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     4321 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     4381 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     4441 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
     4501 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     4561 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     4621 cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg
     4681 cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa
     4741 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg
     4801 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
     4861 agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat
     4921 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     4981 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5041 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5101 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     5161 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     5221 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     5281 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     5341 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     5401 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     5461 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     5521 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     5581 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     5641 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     5701 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     5761 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     5821 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     5881 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     5941 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     6001 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     6061 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     6121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
     6181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     6241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     6301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
     6361 agcgctaccg gtcgccacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa
     6421 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
     6481 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     6541 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
     6601 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
     6661 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
     6721 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
     6781 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
     6841 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
     6901 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
     6961 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac
     7021 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct
     7081 gtacaagtcc ggactcagat ctatggccgc cgctgatgct gagcaggcag ttctggccaa
     7141 gcaagagacc aagcaggatt gctgcatgaa aactgagctg ctaagggaag atacacctat
     7201 ggctgctgat gaaggttcca cagagaaaca agaaggagag actcccatgg ctgcagatgg
     7261 agaaacaaat gggtcttgtg aaaagagtgg ggatcccagc catctaaatg cacccaaaca
     7321 cactcaggag aacacaagag ctagcccaca ggaaggcacc aacagagtgt ctcgggtggc
     7381 agaaaatggg gtttcagaaa gagacacaga agtggggaag caaaaccatg tcacagctga
     7441 cgacttcatg cagacatctg tcattggcag caatggatat ttcttaaata aaccagccct
     7501 gcaggggcag ccgttgagga ctcccaacat tctaacctcc tcgcttcctg gtcatgctgc
     7561 aaaaactctt cctggaggag ccagtaaatg caggactctg agtgcacttc ctcagacacc
     7621 aaccacagca cccactgtgc ctggggaagg gagtgcagac acagaggaca gaaagcctac
     7681 agcctcgggc actgatgtca gggttcacag ggcacgcaag accatgccga agtccatctt
     7741 gggcctgcat gcagccagca aagaccatag agaagttcaa gaccataagg aaccaaaaga
     7801 ggatatcaac agaaacattt ctgaatgtgg acgacagcag cttttaccaa ccttcccagc
     7861 cctccaccag tcgctacctc agaatcagtg ctacatggcc accacaaagt cccagacagc
     7921 agctgcagta tctcggaaga aaaaacgaag aatgggaacc tatagtttag ttcccaagaa
     7981 aaagaccaaa gtattaaaac agaggacggt gattgagatg tttaaaagca taacccattc
     8041 cactgtgggc gccaagggcg agaaagcctt agatgatagt gccctgcatg taaatggcga
     8101 gagcttggag atggactcag aagatgaaga ctccgatgag ctggaggatg acgaggacca
     8161 tggagctgag caagcggctg catttcccac cgaggatagt aggacttcta aagagagcat
     8221 gtctgagact gaccgggctg caaagatgga tggagattca gaggaggaac aggagtctcc
     8281 cgacacaggg gaggatgaag atggtggaga cgagtctgac ctgagttctg aatccagtat
     8341 caagaagaaa tttctcaaga ggagagggaa gactgacagc ccctggatca aacctgctcg
     8401 gaaaaggagg cggagaag
//
