LOCUS       sequence-456376-        8958 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of  RAF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8958)
  AUTHORS   Noeparast A, Giron P, Noor A, Bahadur Shahi R, De Brakeleer S, 
            Eggermont C, Vandenplas H, Boeckx B, Lambrechts D, De Greve J, 
            Teugels E
  TITLE     CRAF mutations in lung cancer can be oncogenic and predict 
            sensitivity to combined type II RAF and MEK inhibition.
  JOURNAL   Oncogene. 2019 Aug;38(31):5933-5941. doi: 10.1038/s41388-019-0866-7.
            Epub 2019 Jul 8.
  PUBMED    31285551
REFERENCE   2  (bases 1 to 8958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41388-019-0866-7"; journalName: "Oncogene"; date: 
            "2019-08"; volume: "38"; issue: "31"; pages: "5933-5941"
COMMENT     Sequence Label: pLenti-puro/RAF1
FEATURES             Location/Qualifiers
     source          1..8958
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        3174..3377
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3328..3348
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    3379..3397
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3400..3418
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             5450..5467
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5490..5507)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(5519..5539)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5524..5853
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5704..5839
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5863..6462
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5863..5882)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6359..6379
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6534..6767
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6839..6960
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6876..6895)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6930..6949
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7000..7135
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7155..7174)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7156..7174)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7184..7201)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7184..7200)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7193..7215)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7342..7797
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7429..7448)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7639..7660
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7823..7927
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7928..7996,7997..8788)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8146..8165)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2821 attcgattag tgaacggatc tcgacggtat cgataagctt gggagttccg cttacataac
     2881 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     2941 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     3001 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     3061 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     3121 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     3181 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     3241 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     3301 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     3361 tctatataag cagagctctc cctatcagtg atagagatct ccctatcagt gatagagatc
     3421 gtcgactagt ggatccagtg tggtggaatt ctgcagatat ccagcacagt ggcggccgct
     3481 cgagtctaga atggagcaca tacagggagc ttggaagacg atcagcaatg gttttggatt
     3541 caaagatgcc gtgtttgatg gctccagctg catctctcct acaatagttc agcagtttgg
     3601 ctatcagcgc cgggcatcag atgatggcaa actcacagat ccttctaaga caagcaacac
     3661 tatccgtgtt ttcttgccga acaagcaaag aacagtggtc aatgtgcgaa atggaatgag
     3721 cttgcatgac tgccttatga aagcactcaa ggtgaggggc ctgcaaccag agtgctgtgc
     3781 agtgttcaga cttctccacg aacacaaagg taaaaaagca cgcttagatt ggaatactga
     3841 tgctgcgtct ttgattggag aagaacttca agtagatttc ctggatcatg ttcccctcac
     3901 aacacacaac tttgctcgga agacgttcct gaagcttgcc ttctgtgaca tctgtcagaa
     3961 attcctgctc aatggatttc gatgtcagac ttgtggctac aaatttcatg agcactgtag
     4021 caccaaagta cctactatgt gtgtggactg gagtaacatc agacaactct tattgtttcc
     4081 aaattccact attggtgata gtggagtccc agcactacct tctttgacta tgcgtcgtat
     4141 gcgagagtct gtttccagga tgcctgttag ttctcagcac agatattcta cacctcacgc
     4201 cttcaccttt aacacctcca gtccctcatc tgaaggttcc ctctcccaga ggcagaggtc
     4261 gacatccaca cctaatgtcc acatggtcag caccaccctg cctgtggaca gcaggatgat
     4321 tgaggatgca attcgaagtc acagcgaatc agcctcacct tcagccctgt ccagtagccc
     4381 caacaatctg agcccaacag gctggtcaca gccgaaaacc cccgtgccag cacaaagaga
     4441 gcgggcacca gtatctggga cccaggagaa aaacaaaatt aggcctcgtg gacagagaga
     4501 ttcaagctat tattgggaaa tagaagccag tgaagtgatg ctgtccactc ggattgggtc
     4561 aggctctttt ggaactgttt ataagggtaa atggcacgga gatgttgcag taaagatcct
     4621 aaaggttgtc gacccaaccc cagagcaatt ccaggccttc aggaatgagg tggctgttct
     4681 gcgcaaaaca cggcatgtga acattctgct tttcatgggg tacatgacaa aggacaacct
     4741 ggcaattgtg acccagtggt gcgagggcag cagcctctac aaacacctgc atgtccagga
     4801 gaccaagttt cagatgttcc agctaattga cattgcccgg cagacggctc agggaatgga
     4861 ctatttgcat gcaaagaaca tcatccatag agacatgaaa tccaacaata tatttctcca
     4921 tgaaggctta acagtgaaaa ttggagattt tggtttggca acagtaaagt cacgctggag
     4981 tggttctcag caggttgaac aacctactgg ctctgtcctc tggatggccc cagaggtgat
     5041 ccgaatgcag gataacaacc cattcagttt ccagtcggat gtctactcct atggcatcgt
     5101 attgtatgaa ctgatgacgg gggagcttcc ttattctcac atcaacaacc gagatcagat
     5161 catcttcatg gtgggccgag gatatgcctc cccagatctt agtaagctat ataagaactg
     5221 ccccaaagca atgaagaggc tggtagctga ctgtgtgaag aaagtaaagg aagagaggcc
     5281 tctttttccc cagatcctgt cttccattga gctgctccaa cactctctac cgaagatcaa
     5341 ccggagcgct tccgagccat ccttgcatcg ggcagcccac actgaggata tcaatgcttg
     5401 cacgctgacc acgtccccga ggctgcctgt cttctagacg cgtaccggtc atcatcacca
     5461 tcaccattga gtttaaacct gctgatcagc ctcgactgtg ccttctagtc gaccctgtgg
     5521 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     5581 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     5641 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     5701 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     5761 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     5821 ggaggctttt ttggaggcct aggcttttgc aaaaagctta ccatgaccga gtacaagccc
     5881 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     5941 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     6001 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     6061 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     6121 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     6181 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     6241 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     6301 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     6361 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     6421 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaggtacctt taagaccaat
     6481 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     6541 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
     6601 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     6661 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     6721 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     6781 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     6841 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     6901 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     6961 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     7021 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     7081 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg
     7141 acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
     7201 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     7261 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     7321 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     7381 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     7441 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     7501 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     7561 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     7621 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     7681 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     7741 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     7801 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     7861 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     7921 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     7981 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     8041 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     8101 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     8161 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     8221 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     8281 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     8341 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     8401 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     8461 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     8521 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     8581 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     8641 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     8701 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     8761 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     8821 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     8881 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     8941 aaagatcaaa ggatcttc
//
