LOCUS       sequence-456387-       11509 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Capture plasmid with apramycin resistance and pSG5 replicon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11509)
  AUTHORS   Xie F, Zhao H, Liu J, Yang X, Neuber M, Agrawal AA, Kaur A, Herrmann
            J, Kalinina OV, Wei X, Muller R, Fu C
  TITLE     Autologous DNA mobilization and multiplication expedite natural 
            products discovery from bacteria.
  JOURNAL   Science. 2024 Dec 13;386(6727):eabq7333. doi: 
            10.1126/science.abq7333. Epub 2024 Dec 13.
  PUBMED    39666857
REFERENCE   2  (bases 1 to 11509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2024-12-13"; volume: "386(6727):eabq7333. doi"; pages: " 
            10.1126/science.abq7333. Epub 2024 Dec 13"
COMMENT     Sequence Label: pCap-SG5-Apr
FEATURES             Location/Qualifiers
     source          1..11509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      285..816
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      293..903
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     CDS             1982..3424
                     /codon_start=1
                     /gene="rep"
                     /product="replication initiator protein from the 
                     Streptomyces ghanaensis plasmid pSG5"
                     /label=pSG5 Rep
                     /translation="MWKPGEATWGNTCRCNNVHTCPWCMSRILAVRGSNVQLAADGLAD
                     AGYGLHLGTNTLRHFERMAFGTVRKGMRHGLVAVLHDGWKGAYGSSGRRWRTMRDDFGI
                     IGYERAFEDTFGWGSGWHLHWHTLWVTREVLGPDAQAAFRDALAGAWAAGVESAGGYTV
                     SETCDRPGCSCEGKGHGTDVRPLNGADAADGDAGKQARYLYKDGDKTKGGVAKIGLELA
                     GQNFKAGRGDDRMGPLDLGDAAAAELQRLRRPGPFVEKYREREFGVFQVRKHYRSQNLN
                     RLIKELGIQQDVRTEEEITDDTEGLVAIAVIPAYIWYRYIARVAGRRLDLIKVAETYGL
                     PGVRRLVESWGLVWGKDVLDPPAPEAPAAPGDLDADQMRFEVMSEEEAAFREARRKANE
                     ARTEELAASLDRVRQPKKEAIRPTISLRKRLKPKPVTVDVKTPPPGAASPVCRRCKGKL
                     APVLQPWGQHPGDCLRVDTAVA"
     oriT            4086..4195
                     /note="incP origin of transfer"
     CDS             4511..5311
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDSFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELLAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      complement(5555..6143)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5635..5654)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6314..7105,7106..7174))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6937..6956
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7175..7279)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    7390..7863
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(7936..8907)
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(8907..10082)
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     misc_feature    10408..10658
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(10661..11416)
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      join(11507..11509,1..217)
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
ORIGIN
        1 cttctgaggg caatttgtca cagggttaag ggcaatttgt cacagacagg actgtcattt
       61 gagggtgatt tgtcacactg aaagggcaat ttgtcacaac accttctcta gaaccagcat
      121 ggataaaggc ctacaaggcg ctctaaaaaa gaagatctaa aaactataaa aaaaataatt
      181 ataaaaatat ccccgtggat aagtggataa ccccaaggga agttttttca ggcatcgtgt
      241 gtaagcagaa tatataagtg ctgttccctg gtgcttcctc gctcactcga ccgggagggt
      301 tcgagaaggg ggggcacccc ccttcggcgt gcgcggtcac gcgcacaggg cgcagccctg
      361 gttaaaaaca aggtttataa atattggttt aaaagcaggt taaaagacag gttagcggtg
      421 gccgaaaaac gggcggaaac ccttgcaaat gctggatttt ctgcctgtgg acagcccctc
      481 aaatgtcaat aggtgcgccc ctcatctgtc agcactctgc ccctcaagtg tcaaggatcg
      541 cgcccctcat ctgtcagtag tcgcgcccct caagtgtcaa taccgcaggg cacttatccc
      601 caggcttgtc cacatcatct gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg
      661 cgaggctggc cagctccacg tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc
      721 cgcgccgggt gagtcggccc ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc
      781 caagttttcc gcgaggtatc cacaacgccg gcggccgcgg tgtctcgcac acggcttcga
      841 cggcgtttct ggcgcgtttg cagggccata gacggccgcc agcccagcgg cgagggcaac
      901 cagccagatc cgaaaacccc aagttacgga tctgagggct tcgccctgtc gctcaactgc
      961 ggcgagcact actggctgta aaaggacaga ccacatcatg gttctgtgtt cattaggttg
     1021 ttctgtccat tgctgacata atccgctcca cttcaacgta acaccgcacg aagatttcta
     1081 ttgttcctga aggcatattc aaatcgtttt cgttaccgct tgcaggcatc atgacagaac
     1141 actacttcct ataaacgcta cacaggctcc tgagattaat aatgcggatc tctacgataa
     1201 tgggagattt tcccgactgt ttcgttcgct tctcagtgga taacagccag cttctctgtt
     1261 taacagacaa aaacagcata tccactcagt tccacatttc catataaagg ccaaggcatt
     1321 tattctcagg ataattgttt cagcatcgca accgcatcag actccggcat cgcaaactgc
     1381 acccggtgcc gggcagccac atccagcgca aaaaccttcg tgtagacttc cgttgaactg
     1441 atggacttat gtcccatcag gctttgcaga actttcagcg gtataccggc atacagcatg
     1501 tgcatcgcat aggaatggcg gaacgtatgt ggtgtgaccg gaacagagaa cgtcacaccg
     1561 tcagcagcag cggcggcaac cgcctcccca atccaggtcc tgaccgttct gtccgtcact
     1621 tcccagatcc gcgctttctc tgtccttcct gtgcgacggt tacgccgctc catgagctta
     1681 tcgcgaataa atacctgtga cggaagatca cttcgcagaa taaataaatc ctggtgtccc
     1741 tgttgatacc gggaagccct gggccaactt ttggcgaaaa tgagacgttg atcggcacgt
     1801 aagaggttcc aactttcacc ataatgaaat aagatcacta ccggacgagg gtgccgcgac
     1861 agccgggcgg gaggacccgc gcgacgagcg gttcgcccgg cgggaccggc accagacggt
     1921 gtcgctgaac gaggcgttca agggctgcgg gacccggctc actgccggga cggccgagct
     1981 gatgtggaag ccgggcgagg cgacgtgggg caacacgtgc cgctgcaaca acgtccacac
     2041 ctgcccgtgg tgcatgagcc gcatcctggc cgtacggggc tcgaacgtgc agctcgccgc
     2101 cgacggtctc gcggacgccg ggtacgggct gcaccttggc acgaacacgt tgcgccactt
     2161 cgagcgcatg gcgttcggca ccgtccgcaa gggaatgcgg cacggcctgg tcgcggtctt
     2221 gcacgacggc tggaagggcg cgtacggctc gtccggccgc cgctggcgca cgatgcgcga
     2281 cgacttcggg atcatcggct acgagcgggc cttcgaggac accttcggct ggggctccgg
     2341 ctggcacctg cactggcaca cgctctgggt cacccgcgag gtgctcggcc cggacgccca
     2401 ggccgcgttc cgcgacgcac tcgccggagc ctgggccgcc ggggtcgaat ccgcgggcgg
     2461 ctacaccgtc agcgagacgt gcgaccggcc cggttgctcc tgtgagggca agggccacgg
     2521 caccgacgtg cgcccactca acggcgcgga cgccgctgac ggcgatgcgg gcaagcaggc
     2581 ccgctacctc tacaaggacg gcgacaagac caagggcggc gtcgccaaga tcggcctgga
     2641 gctggccgga cagaacttca aggccggtcg cggcgacgac cgcatgggac cgctcgacct
     2701 gggcgacgcg gcggccgccg agctgcagcg gcttcgtcgg cccggcccgt tcgtcgagaa
     2761 gtaccgcgag cgcgagttcg gcgtcttcca ggtccgcaag cactaccggt cgcagaacct
     2821 gaaccggctg atcaaggaac tcggcatcca gcaggacgtc cgcaccgagg aggagatcac
     2881 cgacgacacc gagggcctgg tcgccatcgc ggtcatcccg gcctacatct ggtaccgata
     2941 catcgcccgc gtcgccggac gtcgcctcga cctcatcaag gtcgccgaga cgtacggcct
     3001 accgggcgtc cggcggctcg tcgagtcctg gggcctggtg tggggcaagg acgtcctgga
     3061 cccgcccgcc ccggaagcgc cggccgcgcc gggcgacctt gacgctgacc agatgaggtt
     3121 cgaggtcatg tccgaggagg aagctgcttt ccgcgaggcg cgccggaagg ccaacgaggc
     3181 ccgcacggag gagctggccg cttcgctcga ccgagtgcgt cagccgaaga aggaggcgat
     3241 ccgcccgacg atcagccttc ggaagcgcct gaagccgaag ccggtcaccg tcgacgtcaa
     3301 gactcccccg cccggcgccg cgtcgcccgt gtgccgccgc tgcaagggca agctcgcccc
     3361 ggtactccag ccctggggcc agcaccccgg agactgcctc cgcgtcgaca cagcggtcgc
     3421 gtagggccgc tgagggaccg ccatgagacc gccaccccgg acatccgggt ggcggtctca
     3481 tggcggtccc ggcgcgaaag ccgagggtct gcctgccgtg aggtggccgg cggacctggg
     3541 ttactgctgg aatcccgcgc ccagtccgag cgtccgccgg cgcttttcac ctggctccgg
     3601 gtgctcgtcg atgagcacca ggccgtcgac gacgacgcgt ccgcctgcct gcttttggcc
     3661 ggcgggccgg gccgcggttg tccgccgggt cggaccggtc ccttggtcga ccgatgcctc
     3721 ggccacgacg atgctgggcc cgaagcggta cgcgacccgt gccgggccgc agcggatcgc
     3781 ggcagcagga gctccccgcc cgccacggct ggctggatgg tcgcctcacg cgcccgcagg
     3841 ggtgacggcg tgggtgcggc ggtcagcccc gtgatcgggc gtcagacggc cgctgaggga
     3901 ccgcgaccac ccctcccttc ccgcctgccg tctttcaccg cccggccggt ccgcagacgc
     3961 cgtacggcca ggccacggag gattacgggc agatggcgcc cacccggcac ccgggctggg
     4021 gcaccctgga cagcacgaag gtccgcccca ctcaccgggg cgggccttcg cgttgagcta
     4081 gagttcccgc cagcctcgca gagcaggatt cccgttgagc accgccaggt gcgaataagg
     4141 gacagtgaag aaggaacacc cgctcgcggg tgggcctact tcacctatcc tgcccggctg
     4201 acgccgttgg atacaccaag gaaagtctac acgaaccctt tggcaaaatc ctgtatatcg
     4261 tgcgaaaaag gatggatata ccgaaaaaat cgctataatg accccgaagc agggttatgc
     4321 agcggaaaat gcagctcacg gtaactgatg ccgtatttgc agtaccagcg tacggcccac
     4381 agaatgatgt cacgctgaaa atgccggcct ttgaatgggt tcatgtgcag ctccataagc
     4441 aaaaggggat gataagttta tcaccaccga ctatttgcaa cagtgccgtt gatcgtgcta
     4501 tgatcgactg atgtcatcag cggtggagtg caatgtcgtg caatacgaat ggcgaaaagc
     4561 cgagctcatc ggtcagcttc tcaaccttgg ggttaccccc ggcggtgtgc tgctggtcca
     4621 cagctccttc cgtagcgtcc ggcccctcga agatgggcca cttggactga tcgaggccct
     4681 gcgtgctgcg ctgggtccgg gagggacgct cgtcatgccc tcgtggtcag gtctggacga
     4741 cgagccgttc gatcctgcca cgtcgcccgt tacaccggac cttggagttg tctctgactc
     4801 attctggcgc ctgccaaatg taaagcgcag cgcccatcca tttgcctttg cggcagcggg
     4861 gccacaggca gagcagatca tctctgatcc attgcccctg ccacctcact cgcctgcaag
     4921 cccggtcgcc cgtgtccatg aactcgatgg gcaggtactt ctcctcggcg tgggacacga
     4981 tgccaacacg acgctgcatc ttgccgagtt gttggcaaag gttccctatg gggtgccgag
     5041 acactgcacc attcttcagg atggcaagtt ggtacgcgtc gattatctcg agaatgacca
     5101 ctgctgtgag cgctttgcct tggcggacag gtggctcaag gagaagagcc ttcagaagga
     5161 aggtccagtc ggtcatgcct ttgctcggtt gatccgctcc cgcgacattg tggcgacagc
     5221 cctgggtcaa ctgggccgag atccgttgat cttcctgcat ccgccagagg cgggatgcga
     5281 agaatgcgat gccgctcgcc agtcgattgg ctgagctcat aagttcctat tccgaagttc
     5341 ctattcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattg atggagctgc
     5401 acatgaaccg tttaaacctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     5461 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     5521 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     5581 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     5641 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     5701 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     5761 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     5821 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     5881 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     5941 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     6001 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     6061 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     6121 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     6181 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     6241 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     6301 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     6361 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     6421 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     6481 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     6541 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     6601 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     6661 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     6721 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     6781 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     6841 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     6901 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     6961 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     7021 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     7081 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     7141 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     7201 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     7261 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     7321 ttattatcat gacattaacc tataaaaata ggcgtatcac gtttaaactc tcacgctgat
     7381 agtctgatcg acaatacaag tgggaccgtg gtcccagacc gataatcaga ccgacaacac
     7441 gagtgggatc gtggtcccag actaataatc agaccgacga tacgagtggg accgtggtcc
     7501 cagactaata atcagaccga cgatacgagt gggaccgtgg ttccagacta ataatcagac
     7561 cgacgatacg agtgggaccg tggtcccaga ctaataatca gaccgacgat acgagtggga
     7621 ccatggtccc agactaataa tcagaccgac gatacgagtg ggaccgtggt cccagtctga
     7681 ttatcagacc gacgatacga gtgggaccgt ggtcccagac taataatcag accgacgata
     7741 cgagtgggac cgtggtccca gactaataat cagaccgacg atacgagtgg gaccgtggtc
     7801 ccagtctgat tatcagaccg acgatacaag tggaacagtg ggcccagaga gaatattcag
     7861 gccagttatg ctttctggcc tgtaacaaag gacattaagt aaagacagat aaacgtagac
     7921 taaaacgtgg tcgcatcagg gtgctggctt ttcaagttcc ttaagaatgg cctcaatttt
     7981 ctctatacac tcagttggaa cacgagacct gtccaggtta agcaccattt tatcgccctt
     8041 atacaatact gtcgctccag gagcaaactg atgtcgtgag cttaaactag ttcttgatgc
     8101 agatgacgtt ttaagcacag aagttaaaag agtgataact tcttcagctt caaatatcac
     8161 cccagctttt ttctgctcat gaaggttaga tgcctgctgc ttaagtaatt cctctttatc
     8221 tgtaaaggct ttttgaagtg catcacctga ccgggcagat agttcaccgg ggtgagaaaa
     8281 aagagcaaca actgatttag gcaatttggc ggtgttgata cagcgggtaa taatcttacg
     8341 tgaaatattt tccgcatcag ccagcgcaga aatatttcca gcaaattcat tctgcaatcg
     8401 gcttgcataa cgctgaccac gttcataagc acttgttggg cgataatcgt tacccaatct
     8461 ggataatgca gccatctgct catcatccag ctcgccaacc agaacacgat aatcactttc
     8521 ggtaagtgca gcagctttac gacggcgact cccatcggca atttctatga caccagatac
     8581 tcttcgaccg aacgccggtg tctgttgacc agtcagtaga aaagaaggga tgagatcatc
     8641 cagtgcgtcc tcagtaagca gctcctggtc acgttcatta cctgaccata cccgagaggt
     8701 cttctcaaca ctatcacccc ggagcacttc aagagtaaac ttcacatccc gaccacatac
     8761 aggcaaagta atggcattac cgcgagccat tactcctacg cgcgcaatta acgaatccac
     8821 catcggggca gctggtgtcg ataacgaagt atcttcaacc ggttgagtat tgagcgtatg
     8881 ttttggaata acaggcgcac gcttcattat ctaatctccc agcgtggttt aatcagacga
     8941 tcgaaaattt cattgcagac aggttcccaa atagaaagag catttctcca ggcaccagtt
     9001 gaagagcgtt gatcaatggc ctgttcaaaa acagttctca tccggatctg acctttacca
     9061 acttcatccg tttcacgtac aacatttttt agaaccatgc ttccccaggc atcccgaatt
     9121 tgctcctcca tccacgggga ctgagagcca ttactattgc tgtatttggt aagcaaaata
     9181 cgtacatcag gctcgaaccc tttaagatca acgttcttga gcagatcacg aagcatatcg
     9241 aaaaactgca gtgcggaggt gtagtcaaac aactcagcag gcgtgggaac aatcagcaca
     9301 tcagcagcac atacgacatt aatcgtgccg atacccaggt taggcgcgct gtcaataact
     9361 atgacatcat agtcatgagc aacagtttca atggccagtc ggagcatcag gtgtggatcg
     9421 gtgggcagtt taccttcatc aaatttgccc attaactcag tttcaatacg gtgcagagcc
     9481 agacaggaag gaataatgtc aagccccggc cagcaagtgg gctttattgc ataagtgaca
     9541 tcgtcctttt ccccaagata gaaaggcagg agagtgtctt ctgcatgaat atgaagatct
     9601 ggtacccatc cgtgatacat tgaggctgtt ccctgggggt cgttaccttc cacgagcaaa
     9661 acacgtagcc ccttcagagc cagatcctga gcaagatgaa cagaaactga ggttttgtaa
     9721 acgccacctt tatgggcagc aaccccgatc accggtggaa atacgtcttc agcacgtcgc
     9781 aatcgcgtac caaacacatc acgcatatga ttaatttgtt caattgtata accaacacgt
     9841 tgctcaaccc gtcctcgaat ttccatatcc gggtgcggta gtcgccctgc tttctcggca
     9901 tctctgatag cctgagaaga aaccccaact aaatccgctg cttcacctat tctccagcgc
     9961 cgggttattt tcctcgcttc cgggctgtca tcattaaact gtgcaatggc gatagccttc
    10021 gtcatttcat gaccagcgtt tatgcactgg ttaagtgttt ccatgagttt cattctgaac
    10081 atcctttaat cattgctttg cgttttttta ttaaatcttg caatttactg caaagcaaca
    10141 acaaaatcgc aaagtcatca aaaaaccgca aagttgttta aaataagagc aacactacaa
    10201 aaggagataa gaagagcaca tacctcagtc acttattatc actagcgctc gccgcagccg
    10261 tgtaaccgag catagcgagc gaactggcga ggaagcaaag aagaactgtt ctgtcagata
    10321 gctcttacgc tcagcgcaag aagaaatatc caccgtggga aaaactccag gtagaggtac
    10381 acacgcggat agccaattca gagtaataaa ctgtgataat caaccctcat caatgatgac
    10441 gaactaaccc ccgatatcag gtcacatgac gaagggaaag agaaggaaat caactgtgac
    10501 aaactgccct caaatttggc ttccttaaaa attacagttc aaaaagtatg agaaaatcca
    10561 tgcaggctga aggaaacagc aaaactgtga caaattaccc tcagtaggtc agaacaaatg
    10621 tgacgaacca ccctcaaatc tgtgacagat aaccctcaga ctatcctgtc gtcatggaag
    10681 tgatatcgcg gaaggaaaat acgatatgag tcgtctggcg gcctttcttt ttctcaatgt
    10741 atgagaggcg cattggagtt ctgctgttga tctcattaac acagacctgc aggaagcggc
    10801 ggcggaagtc aggcatacgc tggtaacttt gaggcagctg gtaacgctct atgatccagt
    10861 cgattttcag agagacgatg cctgagccat ccggcttacg atactgacac agggattcgt
    10921 ataaacgcat ggcatacgga ttggtgattt cttttgtttc actaagccga aactgcgtaa
    10981 accggttctg taacccgata aagaagggaa tgagatatgg gttgatatgt acactgtaaa
    11041 gccctctgga tggactgtgc gcacgtttga taaaccaagg aaaagattca tagccttttt
    11101 catcgccggc atcctcttca gggcgataaa aaaccacttc cttccccgcg aaactcttca
    11161 atgcctgccg tatatcctta ctggcttccg cagaggtcaa tccgaatatt tcagcatatt
    11221 tagcaacatg gatctcgcag ataccgtcat gttcctgtag ggtgccatca gattttctga
    11281 tctggtcaac gaacagatac agcatacgtt tttgatcccg ggagagacta tatgccgcct
    11341 cagtgaggtc gtttgactgg acgattcgcg ggctattttt acgtttcttg tgattgataa
    11401 ccgctgtttc cgccatgaca gatccatgtg aagtgtgaca agtttttaga ttgtcacact
    11461 aaataaaaaa gagtcaataa gcagggataa ctttgtgaaa aaacagctt
//
