LOCUS       sequence-456389-       11858 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Capture plasmid with hygromycin resistance and pSG5 replicon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11858)
  AUTHORS   Xie F, Zhao H, Liu J, Yang X, Neuber M, Agrawal AA, Kaur A, Herrmann
            J, Kalinina OV, Wei X, Muller R, Fu C
  TITLE     Autologous DNA mobilization and multiplication expedite natural 
            products discovery from bacteria.
  JOURNAL   Science. 2024 Dec 13;386(6727):eabq7333. doi: 
            10.1126/science.abq7333. Epub 2024 Dec 13.
  PUBMED    39666857
REFERENCE   2  (bases 1 to 11858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2024-12-13"; volume: "386(6727):eabq7333. doi"; pages: " 
            10.1126/science.abq7333. Epub 2024 Dec 13"
COMMENT     Sequence Label: pCap-SG5-Hgy
FEATURES             Location/Qualifiers
     source          1..11858
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..474
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(547..1518)
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(1518..2693)
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     misc_feature    3019..3269
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(3272..4027)
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      4118..4337
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      4405..4936
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      4413..5023
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     CDS             6102..7544
                     /codon_start=1
                     /gene="rep"
                     /product="replication initiator protein from the 
                     Streptomyces ghanaensis plasmid pSG5"
                     /label=pSG5 Rep
                     /translation="MWKPGEATWGNTCRCNNVHTCPWCMSRILAVRGSNVQLAADGLAD
                     AGYGLHLGTNTLRHFERMAFGTVRKGMRHGLVAVLHDGWKGAYGSSGRRWRTMRDDFGI
                     IGYERAFEDTFGWGSGWHLHWHTLWVTREVLGPDAQAAFRDALAGAWAAGVESAGGYTV
                     SETCDRPGCSCEGKGHGTDVRPLNGADAADGDAGKQARYLYKDGDKTKGGVAKIGLELA
                     GQNFKAGRGDDRMGPLDLGDAAAAELQRLRRPGPFVEKYREREFGVFQVRKHYRSQNLN
                     RLIKELGIQQDVRTEEEITDDTEGLVAIAVIPAYIWYRYIARVAGRRLDLIKVAETYGL
                     PGVRRLVESWGLVWGKDVLDPPAPEAPAAPGDLDADQMRFEVMSEEEAAFREARRKANE
                     ARTEELAASLDRVRQPKKEAIRPTISLRKRLKPKPVTVDVKTPPPGAASPVCRRCKGKL
                     APVLQPWGQHPGDCLRVDTAVA"
     oriT            8206..8315
                     /note="incP origin of transfer"
     rep_origin      complement(10024..10612)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10104..10123)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10783..11574,11575..11643))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11406..11425
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11644..11748)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacaatacaa gtgggaccgt ggtcccagac cgataatcag accgacaaca cgagtgggat
       61 cgtggtccca gactaataat cagaccgacg atacgagtgg gaccgtggtc ccagactaat
      121 aatcagaccg acgatacgag tgggaccgtg gttccagact aataatcaga ccgacgatac
      181 gagtgggacc gtggtcccag actaataatc agaccgacga tacgagtggg accatggtcc
      241 cagactaata atcagaccga cgatacgagt gggaccgtgg tcccagtctg attatcagac
      301 cgacgatacg agtgggaccg tggtcccaga ctaataatca gaccgacgat acgagtggga
      361 ccgtggtccc agactaataa tcagaccgac gatacgagtg ggaccgtggt cccagtctga
      421 ttatcagacc gacgatacaa gtggaacagt gggcccagag agaatattca ggccagttat
      481 gctttctggc ctgtaacaaa ggacattaag taaagacaga taaacgtaga ctaaaacgtg
      541 gtcgcatcag ggtgctggct tttcaagttc cttaagaatg gcctcaattt tctctataca
      601 ctcagttgga acacgagacc tgtccaggtt aagcaccatt ttatcgccct tatacaatac
      661 tgtcgctcca ggagcaaact gatgtcgtga gcttaaacta gttcttgatg cagatgacgt
      721 tttaagcaca gaagttaaaa gagtgataac ttcttcagct tcaaatatca ccccagcttt
      781 tttctgctca tgaaggttag atgcctgctg cttaagtaat tcctctttat ctgtaaaggc
      841 tttttgaagt gcatcacctg accgggcaga tagttcaccg gggtgagaaa aaagagcaac
      901 aactgattta ggcaatttgg cggtgttgat acagcgggta ataatcttac gtgaaatatt
      961 ttccgcatca gccagcgcag aaatatttcc agcaaattca ttctgcaatc ggcttgcata
     1021 acgctgacca cgttcataag cacttgttgg gcgataatcg ttacccaatc tggataatgc
     1081 agccatctgc tcatcatcca gctcgccaac cagaacacga taatcacttt cggtaagtgc
     1141 agcagcttta cgacggcgac tcccatcggc aatttctatg acaccagata ctcttcgacc
     1201 gaacgccggt gtctgttgac cagtcagtag aaaagaaggg atgagatcat ccagtgcgtc
     1261 ctcagtaagc agctcctggt cacgttcatt acctgaccat acccgagagg tcttctcaac
     1321 actatcaccc cggagcactt caagagtaaa cttcacatcc cgaccacata caggcaaagt
     1381 aatggcatta ccgcgagcca ttactcctac gcgcgcaatt aacgaatcca ccatcggggc
     1441 agctggtgtc gataacgaag tatcttcaac cggttgagta ttgagcgtat gttttggaat
     1501 aacaggcgca cgcttcatta tctaatctcc cagcgtggtt taatcagacg atcgaaaatt
     1561 tcattgcaga caggttccca aatagaaaga gcatttctcc aggcaccagt tgaagagcgt
     1621 tgatcaatgg cctgttcaaa aacagttctc atccggatct gacctttacc aacttcatcc
     1681 gtttcacgta caacattttt tagaaccatg cttccccagg catcccgaat ttgctcctcc
     1741 atccacgggg actgagagcc attactattg ctgtatttgg taagcaaaat acgtacatca
     1801 ggctcgaacc ctttaagatc aacgttcttg agcagatcac gaagcatatc gaaaaactgc
     1861 agtgcggagg tgtagtcaaa caactcagca ggcgtgggaa caatcagcac atcagcagca
     1921 catacgacat taatcgtgcc gatacccagg ttaggcgcgc tgtcaataac tatgacatca
     1981 tagtcatgag caacagtttc aatggccagt cggagcatca ggtgtggatc ggtgggcagt
     2041 ttaccttcat caaatttgcc cattaactca gtttcaatac ggtgcagagc cagacaggaa
     2101 ggaataatgt caagccccgg ccagcaagtg ggctttattg cataagtgac atcgtccttt
     2161 tccccaagat agaaaggcag gagagtgtct tctgcatgaa tatgaagatc tggtacccat
     2221 ccgtgataca ttgaggctgt tccctggggg tcgttacctt ccacgagcaa aacacgtagc
     2281 cccttcagag ccagatcctg agcaagatga acagaaactg aggttttgta aacgccacct
     2341 ttatgggcag caaccccgat caccggtgga aatacgtctt cagcacgtcg caatcgcgta
     2401 ccaaacacat cacgcatatg attaatttgt tcaattgtat aaccaacacg ttgctcaacc
     2461 cgtcctcgaa tttccatatc cgggtgcggt agtcgccctg ctttctcggc atctctgata
     2521 gcctgagaag aaaccccaac taaatccgct gcttcaccta ttctccagcg ccgggttatt
     2581 ttcctcgctt ccgggctgtc atcattaaac tgtgcaatgg cgatagcctt cgtcatttca
     2641 tgaccagcgt ttatgcactg gttaagtgtt tccatgagtt tcattctgaa catcctttaa
     2701 tcattgcttt gcgttttttt attaaatctt gcaatttact gcaaagcaac aacaaaatcg
     2761 caaagtcatc aaaaaaccgc aaagttgttt aaaataagag caacactaca aaaggagata
     2821 agaagagcac atacctcagt cacttattat cactagcgct cgccgcagcc gtgtaaccga
     2881 gcatagcgag cgaactggcg aggaagcaaa gaagaactgt tctgtcagat agctcttacg
     2941 ctcagcgcaa gaagaaatat ccaccgtggg aaaaactcca ggtagaggta cacacgcgga
     3001 tagccaattc agagtaataa actgtgataa tcaaccctca tcaatgatga cgaactaacc
     3061 cccgatatca ggtcacatga cgaagggaaa gagaaggaaa tcaactgtga caaactgccc
     3121 tcaaatttgg cttccttaaa aattacagtt caaaaagtat gagaaaatcc atgcaggctg
     3181 aaggaaacag caaaactgtg acaaattacc ctcagtaggt cagaacaaat gtgacgaacc
     3241 accctcaaat ctgtgacaga taaccctcag actatcctgt cgtcatggaa gtgatatcgc
     3301 ggaaggaaaa tacgatatga gtcgtctggc ggcctttctt tttctcaatg tatgagaggc
     3361 gcattggagt tctgctgttg atctcattaa cacagacctg caggaagcgg cggcggaagt
     3421 caggcatacg ctggtaactt tgaggcagct ggtaacgctc tatgatccag tcgattttca
     3481 gagagacgat gcctgagcca tccggcttac gatactgaca cagggattcg tataaacgca
     3541 tggcatacgg attggtgatt tcttttgttt cactaagccg aaactgcgta aaccggttct
     3601 gtaacccgat aaagaaggga atgagatatg ggttgatatg tacactgtaa agccctctgg
     3661 atggactgtg cgcacgtttg ataaaccaag gaaaagattc atagcctttt tcatcgccgg
     3721 catcctcttc agggcgataa aaaaccactt ccttccccgc gaaactcttc aatgcctgcc
     3781 gtatatcctt actggcttcc gcagaggtca atccgaatat ttcagcatat ttagcaacat
     3841 ggatctcgca gataccgtca tgttcctgta gggtgccatc agattttctg atctggtcaa
     3901 cgaacagata cagcatacgt ttttgatccc gggagagact atatgccgcc tcagtgaggt
     3961 cgtttgactg gacgattcgc gggctatttt tacgtttctt gtgattgata accgctgttt
     4021 ccgccatgac agatccatgt gaagtgtgac aagtttttag attgtcacac taaataaaaa
     4081 agagtcaata agcagggata actttgtgaa aaaacagctt cttctgaggg caatttgtca
     4141 cagggttaag ggcaatttgt cacagacagg actgtcattt gagggtgatt tgtcacactg
     4201 aaagggcaat ttgtcacaac accttctcta gaaccagcat ggataaaggc ctacaaggcg
     4261 ctctaaaaaa gaagatctaa aaactataaa aaaaataatt ataaaaatat ccccgtggat
     4321 aagtggataa ccccaaggga agttttttca ggcatcgtgt gtaagcagaa tatataagtg
     4381 ctgttccctg gtgcttcctc gctcactcga ccgggagggt tcgagaaggg ggggcacccc
     4441 ccttcggcgt gcgcggtcac gcgcacaggg cgcagccctg gttaaaaaca aggtttataa
     4501 atattggttt aaaagcaggt taaaagacag gttagcggtg gccgaaaaac gggcggaaac
     4561 ccttgcaaat gctggatttt ctgcctgtgg acagcccctc aaatgtcaat aggtgcgccc
     4621 ctcatctgtc agcactctgc ccctcaagtg tcaaggatcg cgcccctcat ctgtcagtag
     4681 tcgcgcccct caagtgtcaa taccgcaggg cacttatccc caggcttgtc cacatcatct
     4741 gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg cgaggctggc cagctccacg
     4801 tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc cgcgccgggt gagtcggccc
     4861 ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc caagttttcc gcgaggtatc
     4921 cacaacgccg gcggccgcgg tgtctcgcac acggcttcga cggcgtttct ggcgcgtttg
     4981 cagggccata gacggccgcc agcccagcgg cgagggcaac cagccagatc cgaaaacccc
     5041 aagttacgga tctgagggct tcgccctgtc gctcaactgc ggcgagcact actggctgta
     5101 aaaggacaga ccacatcatg gttctgtgtt cattaggttg ttctgtccat tgctgacata
     5161 atccgctcca cttcaacgta acaccgcacg aagatttcta ttgttcctga aggcatattc
     5221 aaatcgtttt cgttaccgct tgcaggcatc atgacagaac actacttcct ataaacgcta
     5281 cacaggctcc tgagattaat aatgcggatc tctacgataa tgggagattt tcccgactgt
     5341 ttcgttcgct tctcagtgga taacagccag cttctctgtt taacagacaa aaacagcata
     5401 tccactcagt tccacatttc catataaagg ccaaggcatt tattctcagg ataattgttt
     5461 cagcatcgca accgcatcag actccggcat cgcaaactgc acccggtgcc gggcagccac
     5521 atccagcgca aaaaccttcg tgtagacttc cgttgaactg atggacttat gtcccatcag
     5581 gctttgcaga actttcagcg gtataccggc atacagcatg tgcatcgcat aggaatggcg
     5641 gaacgtatgt ggtgtgaccg gaacagagaa cgtcacaccg tcagcagcag cggcggcaac
     5701 cgcctcccca atccaggtcc tgaccgttct gtccgtcact tcccagatcc gcgctttctc
     5761 tgtccttcct gtgcgacggt tacgccgctc catgagctta tcgcgaataa atacctgtga
     5821 cggaagatca cttcgcagaa taaataaatc ctggtgtccc tgttgatacc gggaagccct
     5881 gggccaactt ttggcgaaaa tgagacgttg atcggcacgt aagaggttcc aactttcacc
     5941 ataatgaaat aagatcacta ccggacgagg gtgccgcgac agccgggcgg gaggacccgc
     6001 gcgacgagcg gttcgcccgg cgggaccggc accagacggt gtcgctgaac gaggcgttca
     6061 agggctgcgg gacccggctc actgccggga cggccgagct gatgtggaag ccgggcgagg
     6121 cgacgtgggg caacacgtgc cgctgcaaca acgtccacac ctgcccgtgg tgcatgagcc
     6181 gcatcctggc cgtacggggc tcgaacgtgc agctcgccgc cgacggtctc gcggacgccg
     6241 ggtacgggct gcaccttggc acgaacacgt tgcgccactt cgagcgcatg gcgttcggca
     6301 ccgtccgcaa gggaatgcgg cacggcctgg tcgcggtctt gcacgacggc tggaagggcg
     6361 cgtacggctc gtccggccgc cgctggcgca cgatgcgcga cgacttcggg atcatcggct
     6421 acgagcgggc cttcgaggac accttcggct ggggctccgg ctggcacctg cactggcaca
     6481 cgctctgggt cacccgcgag gtgctcggcc cggacgccca ggccgcgttc cgcgacgcac
     6541 tcgccggagc ctgggccgcc ggggtcgaat ccgcgggcgg ctacaccgtc agcgagacgt
     6601 gcgaccggcc cggttgctcc tgtgagggca agggccacgg caccgacgtg cgcccactca
     6661 acggcgcgga cgccgctgac ggcgatgcgg gcaagcaggc ccgctacctc tacaaggacg
     6721 gcgacaagac caagggcggc gtcgccaaga tcggcctgga gctggccgga cagaacttca
     6781 aggccggtcg cggcgacgac cgcatgggac cgctcgacct gggcgacgcg gcggccgccg
     6841 agctgcagcg gcttcgtcgg cccggcccgt tcgtcgagaa gtaccgcgag cgcgagttcg
     6901 gcgtcttcca ggtccgcaag cactaccggt cgcagaacct gaaccggctg atcaaggaac
     6961 tcggcatcca gcaggacgtc cgcaccgagg aggagatcac cgacgacacc gagggcctgg
     7021 tcgccatcgc ggtcatcccg gcctacatct ggtaccgata catcgcccgc gtcgccggac
     7081 gtcgcctcga cctcatcaag gtcgccgaga cgtacggcct accgggcgtc cggcggctcg
     7141 tcgagtcctg gggcctggtg tggggcaagg acgtcctgga cccgcccgcc ccggaagcgc
     7201 cggccgcgcc gggcgacctt gacgctgacc agatgaggtt cgaggtcatg tccgaggagg
     7261 aagctgcttt ccgcgaggcg cgccggaagg ccaacgaggc ccgcacggag gagctggccg
     7321 cttcgctcga ccgagtgcgt cagccgaaga aggaggcgat ccgcccgacg atcagccttc
     7381 ggaagcgcct gaagccgaag ccggtcaccg tcgacgtcaa gactcccccg cccggcgccg
     7441 cgtcgcccgt gtgccgccgc tgcaagggca agctcgcccc ggtactccag ccctggggcc
     7501 agcaccccgg agactgcctc cgcgtcgaca cagcggtcgc gtagggccgc tgagggaccg
     7561 ccatgagacc gccaccccgg acatccgggt ggcggtctca tggcggtccc ggcgcgaaag
     7621 ccgagggtct gcctgccgtg aggtggccgg cggacctggg ttactgctgg aatcccgcgc
     7681 ccagtccgag cgtccgccgg cgcttttcac ctggctccgg gtgctcgtcg atgagcacca
     7741 ggccgtcgac gacgacgcgt ccgcctgcct gcttttggcc ggcgggccgg gccgcggttg
     7801 tccgccgggt cggaccggtc ccttggtcga ccgatgcctc ggccacgacg atgctgggcc
     7861 cgaagcggta cgcgacccgt gccgggccgc agcggatcgc ggcagcagga gctccccgcc
     7921 cgccacggct ggctggatgg tcgcctcacg cgcccgcagg ggtgacggcg tgggtgcggc
     7981 ggtcagcccc gtgatcgggc gtcagacggc cgctgaggga ccgcgaccac ccctcccttc
     8041 ccgcctgccg tctttcaccg cccggccggt ccgcagacgc cgtacggcca ggccacggag
     8101 gattacgggc agatggcgcc cacccggcac ccgggctggg gcaccctgga cagcacgaag
     8161 gtccgcccca ctcaccgggg cgggccttcg cgttgagcta gagttcccgc cagcctcgca
     8221 gagcaggatt cccgttgagc accgccaggt gcgaataagg gacagtgaag aaggaacacc
     8281 cgctcgcggg tgggcctact tcacctatcc tgcccggctg acgccgttgg atacaccaag
     8341 gaaagtctac acgaaccctt tggcaaaatc ctgtatatcg tgcgaaaaag gatggatata
     8401 ccgaaaaaat cgctataatg accccgaagc agggttatgc agcggaaaat gcagctcacg
     8461 gtaactgatg ccgtatttgc agtaccagcg tacggcccac agaatgatgt cacgctgaaa
     8521 atgccggcct ttgaatgggt tcatgtgcag ctccataagc aaaaggggat gataagttta
     8581 tcaccaccga cttgtaggct ggagctgctt cgatttaaat aatacttgac atatcactgt
     8641 gattcacata taatatgcgc aggttggctg acaaagtgcg taggatgact gtactacaat
     8701 caaaaaaata ataagaaggt aaatcatgac ccaggagtcc ctgctgctgc tggaccgcat
     8761 cgacagcgac gacagctacg cgagcctgcg cagcgaccag gagttctggg agccgctcgc
     8821 ccgccgcgcg ctggaagagc tgggcctgcc ggtcccgccg gtgctgcgcg tgccgggtga
     8881 gtccaccaac ccggtgctgg tgggcgagcc ggacccggtc atcaagctgt tcggggagca
     8941 ctggtgcggc ccggagagcc tggcgagcga gagcgaggcg tacgcggtgc tggccgacgc
     9001 cccggtcccg gtgccgcgcc tgctgggtcg cggcgagctg cgcccaggca cgggtgcctg
     9061 gccgtggccg tacctggtga tgtcccgcat gacgggcacc acgtggcgca gcgcgatgga
     9121 cggcaccacc gaccgcaacg ccctgctggc cctggcgcgc gaactgggtc gcgtcctggg
     9181 tcgcctgcat cgcgtcccgc tgaccggcaa cacggtgctg accccgcact ccgaagtgtt
     9241 cccggagctg ctgcgcgagc gccgcgccgc caccgtcgag gaccaccggg gctggggcta
     9301 cctgagcccg cgcctgctgg accgcctgga agactggctg ccggacgtcg acacgctgct
     9361 ggcgggtcgc gagccgcgct tcgtgcacgg cgacctgcac ggcaccaaca tcttcgtcga
     9421 cctggcggcc accgaggtca ccggcatcgt cgacttcacg gacgtgtacg ccggtgactc
     9481 ccgctactcc ctggtccagc ttcatctgaa cgccttccgc ggtgaccgcg agatcctggc
     9541 ggcgctgctg gacggtgcgc agtggaagcg caccgaggac ttcgcgcgcg agctgctggc
     9601 cttcacgttc ctccacgact tcgaggtctt cgaagaaacc ccgctggacc tgagcggctt
     9661 caccgacccg gaagaactgg cgcagttcct gtggggtccg ccggacacgg cgccgggcgc
     9721 gtgataagat agctcatgag cggagaacga gatgacgttg gaggggcaag gtcgcgctga
     9781 ttgctggggc aacacgtgga gcggatcggg gattgtcttt cttcagctcg ctgatgatat
     9841 gctgacgctc aatgccgtga tggagctgca catgaaccgt ttaaacctgc ggcgagcggt
     9901 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     9961 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
    10021 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
    10081 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
    10141 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
    10201 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
    10261 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
    10321 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
    10381 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
    10441 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
    10501 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
    10561 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
    10621 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
    10681 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
    10741 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
    10801 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
    10861 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
    10921 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
    10981 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    11041 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    11101 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    11161 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    11221 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    11281 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    11341 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    11401 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    11461 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    11521 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    11581 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    11641 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    11701 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    11761 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
    11821 gcgtatcacg tttaaactct cacgctgata gtctgatc
//
