LOCUS       sequence-456400-       11381 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Release plasmid with Cas9 working cassette, hygromycin resistance, 
            and pSG5 replicon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11381)
  AUTHORS   Xie F, Zhao H, Liu J, Yang X, Neuber M, Agrawal AA, Kaur A, Herrmann
            J, Kalinina OV, Wei X, Muller R, Fu C
  TITLE     Autologous DNA mobilization and multiplication expedite natural 
            products discovery from bacteria.
  JOURNAL   Science. 2024 Dec 13;386(6727):eabq7333. doi: 
            10.1126/science.abq7333. Epub 2024 Dec 13.
  PUBMED    39666857
REFERENCE   2  (bases 1 to 11381)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2024-12-13"; volume: "386(6727):eabq7333. doi"; pages: " 
            10.1126/science.abq7333. Epub 2024 Dec 13"
COMMENT     Sequence Label: pRel-Hyg
FEATURES             Location/Qualifiers
     source          1..11381
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             423..1865
                     /codon_start=1
                     /gene="rep"
                     /product="replication initiator protein from the 
                     Streptomyces ghanaensis plasmid pSG5"
                     /label=pSG5 Rep
                     /translation="MWKPGEATWGNTCRCNNVHTCPWCMSRILAVRGSNVQLAADGLAD
                     AGYGLHLGTNTLRHFERMAFGTVRKGMRHGLVAVLHDGWKGAYGSSGRRWRTMRDDFGI
                     IGYERAFEDTFGWGSGWHLHWHTLWVTREVLGPDAQAAFRDALAGAWAAGVESAGGYTV
                     SETCDRPGCSCEGKGHGTDVRPLNGADAADGDAGKQARYLYKDGDKTKGGVAKIGLELA
                     GQNFKAGRGDDRMGPLDLGDAAAAELQRLRRPGPFVEKYREREFGVFQVRKHYRSQNLN
                     RLIKELGIQQDVRTEEEITDDTEGLVAIAVIPAYIWYRYIARVAGRRLDLIKVAETYGL
                     PGVRRLVESWGLVWGKDVLDPPAPEAPAAPGDLDADQMRFEVMSEEEAAFREARRKANE
                     ARTEELAASLDRVRQPKKEAIRPTISLRKRLKPKPVTVDVKTPPPGAASPVCRRCKGKL
                     APVLQPWGQHPGDCLRVDTAVA"
     oriT            2527..2636
                     /note="incP origin of transfer"
     rep_origin      4465..5052
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4953..4972
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5206..5223
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5340..5361
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5376..5381,5382..5399,5400..5406)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     misc_RNA        5614..5689
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        5908..6012
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6013..6081,6082..6873)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6231..6250)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             7139..11245
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7139..11242
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 tttgctctgt gacgagcgta cgagagaaca gcgcgtcgac accaccgcgc cccgccggac
       61 cgaagtgcca ctgcggcacc ccgatcgtcc acatccccgg gaagcgcccc aaaatctact
      121 gctcggacgc ctgccgaaag cgcgcaaagc gtctcattgc ggaccagtca cgcagcgtag
      181 caagcacgac tacagcgggt aatccggacg cgggctttag tagtaaggga aaatctccgt
      241 ccactgcagg tcagagcgct gtaactggaa cgggttctac cccaaacgag ggtgccgcga
      301 cagccgggcg ggaggacccg cgcgacgagc ggttcgcccg gcgggaccgg caccagacgg
      361 tgtcgctgaa cgaggcgttc aagggctgcg ggacccggct cactgccggg acggccgagc
      421 tgatgtggaa gccgggcgag gcgacgtggg gcaacacgtg ccgctgcaac aacgtccaca
      481 cctgcccgtg gtgcatgagc cgcatcctgg ccgtacgggg ctcgaacgtg cagctcgccg
      541 ccgacggtct cgcggacgcc gggtacgggc tgcaccttgg cacgaacacg ttgcgccact
      601 tcgagcgcat ggcgttcggc accgtccgca agggaatgcg gcacggcctg gtcgcggtct
      661 tgcacgacgg ctggaagggc gcgtacggct cgtccggccg ccgctggcgc acgatgcgcg
      721 acgacttcgg gatcatcggc tacgagcggg ccttcgagga caccttcggc tggggctccg
      781 gctggcacct gcactggcac acgctctggg tcacccgcga ggtgctcggc ccggacgccc
      841 aggccgcgtt ccgcgacgca ctcgccggag cctgggccgc cggggtcgaa tccgcgggcg
      901 gctacaccgt cagcgagacg tgcgaccggc ccggttgctc ctgtgagggc aagggccacg
      961 gcaccgacgt gcgcccactc aacggcgcgg acgccgctga cggcgatgcg ggcaagcagg
     1021 cccgctacct ctacaaggac ggcgacaaga ccaagggcgg cgtcgccaag atcggcctgg
     1081 agctggccgg acagaacttc aaggccggtc gcggcgacga ccgcatggga ccgctcgacc
     1141 tgggcgacgc ggcggccgcc gagctgcagc ggcttcgtcg gcccggcccg ttcgtcgaga
     1201 agtaccgcga gcgcgagttc ggcgtcttcc aggtccgcaa gcactaccgg tcgcagaacc
     1261 tgaaccggct gatcaaggaa ctcggcatcc agcaggacgt ccgcaccgag gaggagatca
     1321 ccgacgacac cgagggcctg gtcgccatcg cggtcatccc ggcctacatc tggtaccgat
     1381 acatcgcccg cgtcgccgga cgtcgcctcg acctcatcaa ggtcgccgag acgtacggcc
     1441 taccgggcgt ccggcggctc gtcgagtcct ggggcctggt gtggggcaag gacgtcctgg
     1501 acccgcccgc cccggaagcg ccggccgcgc cgggcgacct tgacgctgac cagatgaggt
     1561 tcgaggtcat gtccgaggag gaagctgctt tccgcgaggc gcgccggaag gccaacgagg
     1621 cccgcacgga ggagctggcc gcttcgctcg accgagtgcg tcagccgaag aaggaggcga
     1681 tccgcccgac gatcagcctt cggaagcgcc tgaagccgaa gccggtcacc gtcgacgtca
     1741 agactccccc gcccggcgcc gcgtcgcccg tgtgccgccg ctgcaagggc aagctcgccc
     1801 cggtactcca gccctggggc cagcaccccg gagactgcct ccgcgtcgac acagcggtcg
     1861 cgtagggccg ctgagggacc gccatgagac cgccaccccg gacatccggg tggcggtctc
     1921 atggcggtcc cggcgcgaaa gccgagggtc tgcctgccgt gaggtggccg gcggacctgg
     1981 gttactgctg gaatcccgcg cccagtccga gcgtccgccg gcgcttttca cctggctccg
     2041 ggtgctcgtc gatgagcacc aggccgtcga cgacgacgcg tccgcctgcc tgcttttggc
     2101 cggcgggccg ggccgcggtt gtccgccggg tcggaccggt cccttggtcg accgatgcct
     2161 cggccacgac gatgctgggc ccgaagcggt acgcgacccg tgccgggccg cagcggatcg
     2221 cggcagcagg agctccccgc ccgccacggc tggctggatg gtcgcctcac gcgcccgcag
     2281 gggtgacggc gtgggtgcgg cggtcagccc cgtgatcggg cgtcagacgg ccgctgaggg
     2341 accgcgacca cccctccctt cccgcctgcc gtctttcacc gcccggccgg tccgcagacg
     2401 ccgtacggcc aggccacgga ggattacggg cagatggcgc ccacccggca cccgggctgg
     2461 ggcaccctgg acagcacgaa ggtccgcccc actcaccggg gcgggccttc gcgttgagct
     2521 agagttcccg ccagcctcgc agagcaggat tcccgttgag caccgccagg tgcgaataag
     2581 ggacagtgaa gaaggaacac ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct
     2641 gacgccgttg gatacaccaa ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc
     2701 gtgcgaaaaa ggatggatat accgaaaaaa tcgctataat gaccccgaag cagggttatg
     2761 cagcggaaaa tgcagctcac ggtaactgat gccgtatttg cagtaccagc gtacggccca
     2821 cagaatgatg tcacgctgaa aatgccggcc tttgaatggg ttcatgtgca gctccataag
     2881 caaaagggga tgataagttt atcaccaccg actatttgca atgtaggctg gagctgcttc
     2941 gatttaaata atacttgaca tatcactgtg attcacatat aatatgcgca ggttggctga
     3001 caaagtgcgt aggatgactg tactacaatc aaaaaaataa taagaaggta aatcatgacc
     3061 caggagtccc tgctgctgct ggaccgcatc gacagcgacg acagctacgc gagcctgcgc
     3121 agcgaccagg agttctggga gccgctcgcc cgccgcgcgc tggaagagct gggcctgccg
     3181 gtcccgccgg tgctgcgcgt gccgggtgag tccaccaacc cggtgctggt gggcgagccg
     3241 gacccggtca tcaagctgtt cggggagcac tggtgcggcc cggagagcct ggcgagcgag
     3301 agcgaggcgt acgcggtgct ggccgacgcc ccggtcccgg tgccgcgcct gctgggtcgc
     3361 ggcgagctgc gcccaggcac gggtgcctgg ccgtggccgt acctggtgat gtcccgcatg
     3421 acgggcacca cgtggcgcag cgcgatggac ggcaccaccg accgcaacgc cctgctggcc
     3481 ctggcgcgcg aactgggtcg cgtcctgggt cgcctgcatc gcgtcccgct gaccggcaac
     3541 acggtgctga ccccgcactc cgaagtgttc ccggagctgc tgcgcgagcg ccgcgccgcc
     3601 accgtcgagg accaccgggg ctggggctac ctgagcccgc gcctgctgga ccgcctggaa
     3661 gactggctgc cggacgtcga cacgctgctg gcgggtcgcg agccgcgctt cgtgcacggc
     3721 gacctgcacg gcaccaacat cttcgtcgac ctggcggcca ccgaggtcac cggcatcgtc
     3781 gacttcacgg acgtgtacgc cggtgactcc cgctactccc tggtccagct tcatctgaac
     3841 gccttccgcg gtgaccgcga gatcctggcg gcgctgctgg acggtgcgca gtggaagcgc
     3901 accgaggact tcgcgcgcga gctgctggcc ttcacgttcc tccacgactt cgaggtcttc
     3961 gaagaaaccc cgctggacct gagcggcttc accgacccgg aagaactggc gcagttcctg
     4021 tggggtccgc cggacacggc gccgggcgcg tgataagata gctcatgagc ggagaacgag
     4081 atgacgttgg aggggcaagg tcgcgctgat tgctggggca acacgtggag cggatcgggg
     4141 attgtctttc ttcagctcgc tgatgatatg ctgacgctca atgccgtagt tcctattcga
     4201 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     4261 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     4321 cggggatctc atgctggagt tcttcgccca ccccaaaagg atctaggtga agatcctttt
     4381 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     4441 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     4501 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     4561 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     4621 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     4681 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     4741 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gtcgtgcaca
     4801 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     4861 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     4921 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     4981 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     5041 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     5101 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     5161 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     5221 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     5281 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     5341 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     5401 atgttgtgtg gaattgtgag tttaaacgcg gataacaatt tcacacagtc gacttctagt
     5461 gcagctagtg tcgactagag gatccccggg tatgcggtcg atcttgacgg ctggcgagag
     5521 gtgcggggag gatctgaccg acgcggtcca cacgtggcac cgcgatgctg ttgtgggcac
     5581 aatcgtgccg gttggtagga tcgacggcca tgggttttag gtctagaaat agcaagttaa
     5641 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttacgt
     5701 aggcgtcagc tacgatgttc cggggactgc tgatccggtc agcaggtgga agagggactg
     5761 gattccaaag ttctcaatgc tgcttgctgt tcttgaatgg ggggtcgttg acgacgacat
     5821 ggctcgattg gcgcgacaag ttgctgcgat tctcaccaat aaaaaacgcc cggcggcaga
     5881 acaaatccag atggagttca ctagttgcgc ggaaccccta tttgtttatt tttctaaata
     5941 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     6001 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     6061 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     6121 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     6181 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     6241 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     6301 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     6361 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     6421 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     6481 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     6541 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     6601 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     6661 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     6721 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     6781 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     6841 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     6901 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     6961 gataatctca tgaccaaaat gctagctgag gtcattactg gaccggatcg gggatctggg
     7021 ctgagggagc cgacggcacg cggcggctca cggcgtggca cgcggaacgt ccgggcttgc
     7081 acctcacgtc acgtgaggag gcagcgtgga cggcgtcaga gaagggagcg gacaattgat
     7141 ggacaagaag tactccatcg gcctggacat cggcaccaac tccgtcggct gggccgtcat
     7201 caccgacgag tacaaggtcc cgtccaagaa gttcaaggtc ctgggcaaca ccgaccgcca
     7261 ctccatcaag aagaacctga tcggcgccct gctgttcgac tccggcgaga ccgccgaggc
     7321 cacccgcctg aagcgcaccg cccgccgccg ctacacccgc cgcaagaacc gcatctgcta
     7381 cctgcaggag atcttctcca acgagatggc caaggtcgac gactccttct tccaccgcct
     7441 ggaggagtcc ttcctggtcg aggaggacaa gaagcacgag cgccacccga tcttcggcaa
     7501 catcgtcgac gaggtcgcct accacgagaa gtacccgacc atctaccacc tgcgcaagaa
     7561 gctggtcgac tccaccgaca aggccgacct gcgcctgatc tacctggccc tggcccacat
     7621 gatcaagttc cgcggccact tcctgatcga gggcgacctg aacccggaca actccgacgt
     7681 cgacaagctg ttcatccagc tggtccagac ctacaaccag ctgttcgagg agaacccgat
     7741 caacgcctcc ggcgtcgacg ccaaggccat cctgtccgcc cgcctgtcca agtcccgccg
     7801 cctggagaac ctgatcgccc agctgccggg cgagaagaag aacggcctgt tcggcaacct
     7861 gatcgccctg tccctgggcc tgaccccgaa cttcaagtcc aacttcgacc tggccgagga
     7921 cgccaagctg cagctgtcca aggacaccta cgacgacgac ctggacaacc tgctggccca
     7981 gatcggcgac cagtacgccg acctgttcct ggccgccaag aacctgtccg acgccatcct
     8041 gctgtccgac atcctgcgcg tcaacaccga gatcaccaag gccccgctgt ccgcctccat
     8101 gatcaagcgc tacgacgagc accaccagga cctgaccctg ctgaaggccc tggtccgcca
     8161 gcagctgccg gagaagtaca aggagatctt cttcgaccag tccaagaacg gctacgccgg
     8221 ctacatcgac ggcggcgcct cccaggagga gttctacaag ttcatcaagc cgatcctgga
     8281 gaagatggac ggcaccgagg agctgctggt caagctgaac cgcgaggacc tgctgcgcaa
     8341 gcagcgcacc ttcgacaacg gctccatccc gcaccagatc cacctgggcg agctgcacgc
     8401 catcctgcgc cgccaggagg acttctaccc gttcctgaag gacaaccgcg agaagatcga
     8461 gaagatcctg accttccgca tcccgtacta cgtcggcccg ctggcccgcg gcaactcccg
     8521 cttcgcctgg atgacccgca agtccgagga gaccatcacc ccgtggaact tcgaggaggt
     8581 cgtcgacaag ggcgcctccg cccagtcctt catcgagcgc atgaccaact tcgacaagaa
     8641 cctgccgaac gagaaggtcc tgccgaagca ctccctgctg tacgagtact tcaccgtcta
     8701 caacgagctg accaaggtca agtacgtcac cgagggcatg cgcaagccgg ccttcctgtc
     8761 cggcgagcag aagaaggcca tcgtcgacct gctgttcaag accaaccgca aggtcaccgt
     8821 caagcagctg aaggaggact acttcaagaa gatcgagtgc ttcgactccg tcgagatctc
     8881 cggcgtcgag gaccgcttca acgcctccct gggcacctac cacgacctgc tgaagatcat
     8941 caaggacaag gacttcctgg acaacgagga gaacgaggac atcctggagg acatcgtcct
     9001 gaccctgacc ctgttcgagg accgcgagat gatcgaggag cgcctgaaga cctacgccca
     9061 cctgttcgac gacaaggtca tgaagcagct gaagcgccgc cgctacaccg gctggggccg
     9121 cctgtcccgc aagctgatca acggcatccg cgacaagcag tccggcaaga ccatcctgga
     9181 cttcctgaag tccgacggct tcgccaaccg caacttcatg cagctgatcc acgacgactc
     9241 cctgaccttc aaggaggaca tccagaaggc ccaggtctcc ggccagggcg actccctgca
     9301 cgagcacatc gccaacctgg ccggctcccc ggccatcaag aagggcatcc tgcagaccgt
     9361 caaggtcgtc gacgagctgg tcaaggtcat gggccgccac aagccggaga acatcgtcat
     9421 cgagatggcc cgcgagaacc agaccaccca gaagggccag aagaactccc gcgagcgcat
     9481 gaagcgcatc gaggagggca tcaaggagct gggctcccag atcctgaagg agcacccggt
     9541 cgagaacacc cagctgcaga acgagaagct gtacctgtac tacctgcaga acggccgcga
     9601 catgtacgtc gaccaggagc tggacatcaa ccgcctgtcc gactacgacg tcgaccacat
     9661 cgtcccgcag tccttcctga aggacgactc catcgacaac aaggtcctga cccgctccga
     9721 caagaaccgc ggcaagtccg acaacgtccc gtccgaggag gtcgtcaaga agatgaagaa
     9781 ctactggcgc cagctgctga acgccaagct gatcacccag cgcaagttcg acaacctgac
     9841 caaggccgag cgcggcggcc tgtccgagct ggacaaggcc ggcttcatca agcgccagct
     9901 ggtcgagacc cgccagatca ccaagcacgt cgcccagatc ctggactccc gcatgaacac
     9961 caagtacgac gagaacgaca agctgatccg cgaggtcaag gtcatcaccc tgaagtccaa
    10021 gctggtctcc gacttccgca aggacttcca gttctacaag gtccgcgaga tcaacaacta
    10081 ccaccacgcc cacgacgcct acctgaacgc cgtcgtcggc accgccctga tcaagaagta
    10141 cccgaagctg gagtccgagt tcgtctacgg cgactacaag gtctacgacg tccgcaagat
    10201 gatcgccaag tccgagcagg agatcggcaa ggccaccgcc aagtacttct tctactccaa
    10261 catcatgaac ttcttcaaga ccgagatcac cctggccaac ggcgagatcc gcaagcgccc
    10321 gctgatcgag accaacggcg agaccggcga gatcgtctgg gacaagggcc gcgacttcgc
    10381 caccgtccgc aaggtcctgt ccatgccgca ggtcaacatc gtcaagaaga ccgaggtcca
    10441 gaccggcggc ttctccaagg agtccatcct gccgaagcgc aactccgaca agctgatcgc
    10501 ccgcaagaag gactgggacc cgaagaagta cggcggcttc gactccccga ccgtcgccta
    10561 ctccgtcctg gtcgtcgcca aggtcgagaa gggcaagtcc aagaagctga agtccgtcaa
    10621 ggagctgctg ggcatcacca tcatggagcg ctcctccttc gagaagaacc cgatcgactt
    10681 cctggaggcc aagggctaca aggaggtcaa gaaggacctg atcatcaagc tgccgaagta
    10741 ctccctgttc gagctggaga acggccgcaa gcgcatgctg gcctccgccg gcgagctgca
    10801 gaagggcaac gagctggccc tgccgtccaa gtacgtcaac ttcctgtacc tggcctccca
    10861 ctacgagaag ctgaagggct ccccggagga caacgagcag aagcagctgt tcgtcgagca
    10921 gcacaagcac tacctggacg agatcatcga gcagatctcc gagttctcca agcgcgtcat
    10981 cctggccgac gccaacctgg acaaggtcct gtccgcctac aacaagcacc gcgacaagcc
    11041 gatccgcgag caggccgaga acatcatcca cctgttcacc ctgaccaacc tgggcgcccc
    11101 ggccgccttc aagtacttcg acaccaccat cgaccgcaag cgctacacct ccaccaagga
    11161 ggtcctggac gccaccctga tccaccagtc catcaccggc ctgtacgaga cccgcatcga
    11221 cctgtcccag ctgggcggcg actaaggatc ggacgaacaa taaggggagc gggaaaccgc
    11281 tccccttttt tattgataac aaaaaagctt ctcgtcgaag gcactagaag gcctatatgc
    11341 ggggatcgac cgcgcagcat gccgagtgag gacgtttaaa c
//
