LOCUS       sequence-456432-       12960 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12960)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2085
FEATURES             Location/Qualifiers
     source          1..12960
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    5898..6486
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5951..5971)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6369..6380)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6558..6791
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6869..6990
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6906..6925)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6960..6979
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7030..7165
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7185..7204)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7186..7204)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7214..7231)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7214..7230)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7223..7245)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7372..7827
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7459..7478)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7669..7690
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7853..7957
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7958..8026,8027..8818)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8176..8195)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8989..9577
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9478..9497
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9731..9748
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9865..9886
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9901..9906,9907..9924,9925..9931)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9939..9955
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9944..9966
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9963..9979
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9963..9979
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9998..10018
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10000..10018
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10046..10272
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10273..10453
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10500..10625
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11118..11351
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11536..11580
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        11811..12021
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        11833..12029
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      11880..12015
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             12061..12663
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(12061..12080)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     12557..12577
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    12706..12823
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgcc
     1201 ctcggacttc atctcattgc tcagcgcgga cctagacctg gaatcgccca agtccctcta
     1261 ctcgcgagaa tctgtctatg atcttctccc aaaggagtta cagttacctc catctagaga
     1321 aacatctgta gcatcaatga gtcagacaag cggtggtgag gcaggctcgc ctcctccagc
     1381 tgttgttgct gctgatgctt cttcagctcc ctcctcttcc tccatgggcg gtgcttgcag
     1441 ctcctttacc acctcttcca gccctaccat ttattctacc tcagtcaccg acagcaaggc
     1501 tatgcaagtg gagagctgct cctcagccgt gggggtaagt aacagagggg taagtgaaaa
     1561 gcagttaacc agtaacacag ttcagcagca tccatcaaca ccgaagaggc acacagtctt
     1621 gtacatctca ccaccacctg aggacttgct ggataacagt cggatgtcct gccaggatga
     1681 ggggtgtgga ttggaatctg agcagagctg cagtatgtgg atggaggatt ccccctccaa
     1741 cttcagtaac atgagcacca gttcctacaa tgataacact gaggtacctc gtaaatcacg
     1801 aaaacgaaat ccaaagcaga ggccgggggt caaacgacga gattgtgaag aatctaatat
     1861 ggatatattt gatgccgaca gtgccaaagc acctcactat gtgctttctc agcttaccac
     1921 ggacaacaaa ggcaactcaa aagcgggaaa tggaacattg gaaaaccaaa aaggaactgg
     1981 agtaaagaag agccctatgt tgtgtggaca atatcctgtt aaaagtgagg gaaaggagct
     2041 gaagatagtt gtacaacctg agacacagca ccgagctcgg tacctgactg agggcagccg
     2101 tggctcagtg aaagatagaa cacagcaagg ctttcctaca gtaaagctgg aaggccataa
     2161 tgaacctgta gtgttgcaag tgtttgtggg caacgactct ggacgagtga aaccacatgg
     2221 attttatcag gcctgcagag taactggacg aaatacaact ccttgcaaag aagtggacat
     2281 tgaaggcact actgttatag aagtcggcct tgatcctagc aacaacatga cactggcggt
     2341 ggactgcgta gggatattga aattgaggaa tgctgatgtc gaagccagaa taggaattgc
     2401 tggttccaag aagaaaagca ctcgtgccag attggttttt cgagttaata tcatgaggaa
     2461 agatggctcc actttgacac tgcaaacacc ctcttctcca attttgtgta ctcagccagc
     2521 aggagtgcca gaaatcttaa agaaaagctt gcatagctgt tcagtgaaag gagaagaaga
     2581 agtgttttta atcggcaaga actttctgaa aggaactaaa gttattttcc aagaaaatgt
     2641 ttctgatgaa aactcttgga agtcagaagc tgaaattgat atggaactat ttcatcagaa
     2701 tcatcttatt gtgaaggttc ctccctatca tgaccaacat ataactttgc ctgtgtcagt
     2761 gggaatatat gtagtgacaa atgctggaag atctcatgat gttcaaccat tcacttacac
     2821 tccagaccca gcagcagctg gtgctttgaa tgtaaatgtg aagaaggaaa tatctagtcc
     2881 agcaagacct tgctcttttg aagaggccat gaaagcaatg aaaactactg gatgtaattt
     2941 agataaggta aatattatcc ctaatgccct gatgactcca ctcataccaa gcagtatgat
     3001 taagagtgaa gatgttactc caatggaagt aacagcagaa aaaagatctt ccactatttt
     3061 taagactaca aagtctgttg gatcaactca gcaaacatta gaaaacatct caaacatagc
     3121 aggaaatggc tctttttcat caccatcatc ttcccaccta ccttctgaaa atgaaaaaca
     3181 gcagcagatt cagcccaagg catacaaccc agagaccctg acaactattc aaacccagga
     3241 catctcacag cctggtactt ttccagcagt ttctgcttct agtcagctgc ccaacagcga
     3301 tgcactattg cagcaggcta cacagtttca gacaagagaa actcagtcta gagagatatt
     3361 acagtcagat ggtacagtgg ttaatttgtc acaactgact gaggcatcac aacaacagca
     3421 gcagtcacca ctacaagaac aagcacagac tttacagcag cagatttcat caaatatttt
     3481 tccatcacca aatagtgtga gtcagcttca gaatactatt cagcagctgc aagcagggag
     3541 tttcacaggc agtactgcta gtggcagcag tggaagtgtt gacttggtcc aacaagtttt
     3601 agaggcacag cagcagttat cttcagtttt attttctgct ccagatggta atgagaatgt
     3661 tcaagagcag cttagtgcag atatttttca acaagtcagt caaattcaga gtggtgtaag
     3721 ccctggaatg ttttcctcaa cagagccaac agtccatacc agaccagata atttattacc
     3781 tggaagagct gaaagtgttc atccacagtc tgaaaacacg ttatctaatc aacagcagca
     3841 gcagcagcag caacagcaag tgatggaatc ttcagccgca atggtgatgg agatgcaaca
     3901 gagtatctgc caggcagctg cccagattca gtcagagtta ttcccttcaa ctgcttcagc
     3961 aaatggaaac cttcagcaat cgccagttta ccagcagact tctcacatga tgagtgcatt
     4021 gtctaccaat gaggatatgc aaatgcagtg tgaattgttt tcttctcctc ctgcagtttc
     4081 tggaaatgaa acttctacaa ctaccacaca gcaggttgca acccctggca ctaccatgtt
     4141 tcagacatca agttcaggag atggagaaga aactggaaca caagcaaaac agattcagaa
     4201 cagtgtcttt cagaccatgg tccaaatgca acatagtggg gacaatcaac ctcaagttaa
     4261 ccttttttca tccacaaaaa gtatgatgag tgttcagaat agtggtaccc aacaacaagg
     4321 taatggttta ttccagcaag ggaatgagat gatgtcactt caatctggaa attttttgca
     4381 gcagtcttct cattcacagg cccaactttt tcatcctcaa aatcctattg ccgatgctca
     4441 gaacctttcc caggaaactc aaggttctct ctttcatagt ccaaatccta ttgtccacag
     4501 tcagacttct acaacctcct ctgaacaaat gcagcctcca atgtttcact ctcaaagtac
     4561 cattgctgtg ttacagggct cttcagttcc tcaagaccag cagtcaacca acatatttct
     4621 ttcccagagt cccatgaata atcttcagac taacacagta gcccaagaag cattttttgc
     4681 agcaccgaac tcaatttctc cacttcagtc aacatcaaac agtgaacaac aagctgcttt
     4741 ccaacagcaa gctccaatat cacacatcca gactcctatg ctttcccaag aacaggcaca
     4801 acccccgcag cagggtttat ttcagcctca ggtggccctg ggctcccttc cacctaatcc
     4861 aatgcctcaa agccaacaag gaaccatgtt ccagtcacag cactcaatag ttgccatgca
     4921 gagtaactct ccatcccagg aacagcagca gcagcagcaa cagcagcagc aacagcagca
     4981 gcaacaacaa cagagcattt tattcagtaa tcagaatacc atggctacaa tggcgtctcc
     5041 aaagcaacca ccaccaaaca tgatattcaa cccaaatcaa aatccaatgg ctaatcagga
     5101 gcaacagaac cagtcaattt ttcaccaaca aagtaacatg gccccaatga atcaagagca
     5161 acagcccatg caatttcaga gtcagtccac agtttcctca cttcagaacc caggtcctac
     5221 ccagtcggaa tcatcacaga cccccttgtt ccatagctct cctcagattc agttggtaca
     5281 agggtcacct agttctcaag agcagcaagt aactctcttc ttatctccag catccatgtc
     5341 tgccttgcag accagtataa atcaacaaga tatgcaacag tctcctcttt attcccctca
     5401 gaacaacatg cctggaattc aaggagccac atcttcgcct caaccacagg ctactttatt
     5461 tcacaacaca gcaggaggca caatgaacca actgcagaat tctcctggct catctcagca
     5521 gacatcagga atgttcttat ttggcattca aaataactgt agtcagcttt taacctctgg
     5581 accagctaca ttgcctgatc agttgatggc cataagtcag ccaggccaac cacaaaacga
     5641 gggccagcca cctgtgacaa cacttctttc tcagcaaatg ccagagaatt ctccactggc
     5701 atcctctata aacaccaacc agaacatcga aaagattgat ttgcttgttt cattgcaaaa
     5761 ccaagggaac aacttgactg gctcctttta gtaatgaact agtccgtaac ttgaaagtat
     5821 ttcgatttct tggctttata tatcttgtgg aaaggacgag ctcactccga agccaaggaa
     5881 tccacgcgtt aagcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     5941 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     6001 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     6061 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     6121 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     6181 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     6241 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     6301 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     6361 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     6421 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     6481 ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta gccacttttt
     6541 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     6601 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     6661 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     6721 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     6781 aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt tataacttgc
     6841 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     6901 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     6961 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     7021 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     7081 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     7141 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt
     7201 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     7261 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     7321 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
     7381 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     7441 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     7501 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     7561 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     7621 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     7681 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     7741 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     7801 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
     7861 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     7921 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     7981 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     8041 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     8101 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     8161 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     8221 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     8281 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     8341 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     8401 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     8461 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     8521 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     8581 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     8641 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     8701 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     8761 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     8821 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     8881 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     8941 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     9001 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     9061 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     9121 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     9181 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     9241 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     9301 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     9361 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     9421 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     9481 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     9541 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     9601 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     9661 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     9721 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     9781 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     9841 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     9901 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     9961 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
    10021 acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac
    10081 atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga
    10141 ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca
    10201 tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct
    10261 cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
    10321 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
    10381 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
    10441 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
    10501 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
    10561 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
    10621 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
    10681 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
    10741 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
    10801 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
    10861 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
    10921 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
    10981 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
    11041 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
    11101 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
    11161 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
    11221 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
    11281 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
    11341 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
    11401 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
    11461 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
    11521 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
    11581 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
    11641 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
    11701 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccatgcat
    11761 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
    11821 gtatgcaaag catgcgtctc aattagtcag caaccatagt cccgccccta actccgccca
    11881 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
    11941 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
    12001 gcttttttgg aggcctaggc ttttgcaaaa agctttctag aggtaccacc ggagcttacc
    12061 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
    12121 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
    12181 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
    12241 atcggcaagg tgtgggtcgc ggacgacggc gccgccgtgg cggtctggac cacgccggag
    12301 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
    12361 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
    12421 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
    12481 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
    12541 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
    12601 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgccagt
    12661 taacgaattc tagatcttga gacaaatggc agtattcatc cacaatttta aaagaaaagg
    12721 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
    12781 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
    12841 cagcagagat ccactttggc gccggctcga ggcctgcagg tgcaaagatg gataaagttt
    12901 taaacagaga ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt
//
