LOCUS       sequence-456463-        9799 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Homology arms and linker-mEGFP sequence for internal insertion of 
            CAGGS-mEGFP at the AAVS1 safe harbor location in human cells as a 
            reporter for cytoplasmic mEGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9799)
  AUTHORS   Allen Institute for Cell Science
  TITLE     Allen Institute for Cell Science Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AICSDP-35:AAVS1-mEGFP
FEATURES             Location/Qualifiers
     source          1..9799
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..804
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     promoter        complement(839..857)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(878..894)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    902..918
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(926..932,933..950,951..956))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    971..992
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1109..1126)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1280..1868)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1360..1379)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(1992..2060,2061..2852)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2210..2229)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(3213..3232)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3827..3846
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             complement(4249..4551)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(4351..4370)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     4674..4696
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4688..4705
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4689..4705
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4712..4731
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4712..4730
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    4765..5601
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     protein_bind    5675..5696
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5711..5716,5717..5734,5735..5741)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5749..5765
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5773..5789
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6132..6151
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    6150..6205
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     6251..6270
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(6360..7073,7074..7076,7077..7079))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6405..6426)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6754..6773
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7013..7034
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      7076..7085
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(7114..7133)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          7141..8157
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(7195..7217)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     enhancer        8435..8814
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    8820..9044
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     9033..9050
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(9082..9681)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9165..9185)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     9662..9681
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(9691..9744)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    9768..9793
                     /label=SA
                     /note="splice acceptor site"
ORIGIN
        1 gccccactgt ggggtggagg ggacagataa aagtacccag aaccagagcc acattaaccg
       61 gccctgggaa tataaggtgg tcccagctcg gggacacagg atccctggag gcagcaaaca
      121 tgctgtcctg aagtggacat aggggcccgg gttggaggaa gaagactagc tgagctctcg
      181 gacccctgga agatgccatg acagggggct ggaagagcta gcacagacta gagaggtaag
      241 gggggtaggg gagctgccca aatgaaagga gtgagaggtg acccgaatcc acaggagaac
      301 ggggtgtcca ggcaaagaaa gcaagaggat ggagaggtgg ctaaagccag ggagacgggg
      361 tactttgggg ttgtccagaa aaacggtgat gatgcaggcc tacaagaagg ggaggcggga
      421 cgcaagggag acatccgtcg gagaaggcca tcctaagaaa cgagagatgg cacaggcccc
      481 agaaggagaa ggaaaaggga acccagcgag tgaagacggc atggggttgg gtgagggagg
      541 agagatgccc ggagaggacc cagacacggg gaggatccgc tcagaggaca tcacgtggtg
      601 cagcgccgag aaggaagtgc tccggaaaga gcatccttgg gcagcaacac agcagagagc
      661 aaggggaaga gggagtggag gaagacggaa cctgaaggag gcggcaggga aggatctggg
      721 ccagccgtag aggtgaccca ggccacaagc tgcagacaga aagcggcaca ggcccagggg
      781 agagaatgct ggtcagagaa agcaaagggc gaattcgttt aaacctgcag gactagtccc
      841 tttagtgagg gttaattctg agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
      901 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
      961 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     1021 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     1081 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     1141 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     1201 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     1261 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     1321 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1381 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     1441 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     1501 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     1561 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     1621 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     1681 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     1741 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     1801 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1861 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     1921 cacgttaagg gattttggtc atgagacaat aaccctgata aatgcttcaa taatattgaa
     1981 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     2041 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     2101 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     2161 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     2221 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     2281 agaatgactt ggttgagtat tcaccagtca cagaaaagca tcttacggat ggcatgacag
     2341 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     2401 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     2461 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     2521 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     2581 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     2641 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     2701 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     2761 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     2821 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     2881 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     2941 ataatctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     3001 cgcgcacatt tccccgaaaa gtgccacctg atgcggtgtg aaataccgca cagatgcgta
     3061 aggagaaaat accgcatcag gaaattgtaa gcgttaataa ttcagaagaa ctcgtcaaga
     3121 aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag
     3181 cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc
     3241 tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt
     3301 tccaccatga tattcggcaa gcaggcatcg ccatgcatgg gtcacgacga gatcctcgcc
     3361 gtcgggcatg ctcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc
     3421 ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat
     3481 gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg
     3541 cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg acaggagatc
     3601 ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag
     3661 cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcttg
     3721 cagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc
     3781 tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc
     3841 gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatcat
     3901 gcgaaacgat cctcatcctg tctcttgatc agagcttgat cccctgcgcc atcagatcct
     3961 tggcggcaag aaagccatcc agtttacttt gcagggcttc ccaaccttac cagagggcgc
     4021 cccagctggc aattccggtt cgcttgctgt ccataaaacc gcccagtcta gctatcgcca
     4081 tgtaagccca ctgcaagcta cctgctttct ctttgcgctt gcgttttccc ttgtccagat
     4141 agcccagtag ctgacattca tccggggtca gcaccgtttc tgcggactgg ctttctacgt
     4201 gaaaaggatc taggtgaaga tcctttttga taatctcatg cctgacattt atattcccca
     4261 gaacatcagg ttaatggcgt ttttgatgtc attttcgcgg tggctgagat cagccacttc
     4321 ttccccgata acggagaccg gcacactggc catatcggtg gtcatcatgc gccagctttc
     4381 atccccgata tgcaccaccg ggtaaagttc acgggagact ttatctgaca gcagacgtgc
     4441 actggccagg gggatcacca tccgtcgccc cggcgtgtca ataatatcac tctgtacatc
     4501 cacaaacaga cgataacggc tctctctttt ataggtgtaa accttaaact gccgtacgta
     4561 taggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     4621 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
     4681 acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg gcgaattgaa
     4741 tttagcggcc gcgaattcgc ccttagagca gagccaggaa cccctgtagg gaaggggcag
     4801 gagagccagg ggcatgagat ggtggacgag gaagggggac agggaagcct gagcgcctct
     4861 cctgggcttg ccaaggactc aaacccagaa gcccagagca gggccttagg gaagcgggac
     4921 cctgctctgg gcggaggaat atgtcccaga tagcactggg gactctttaa ggaaagaagg
     4981 atggagaaag agaaagggag tagaggcggc cacgacctgg tgaacaccta ggacgcacca
     5041 ttctcacaaa gggagttttc cacacggaca cccccctcct caccacagcc ctgccaggac
     5101 ggggctggct actggcctta tctcacaggt aaaactgacg cacggaggaa caatataaat
     5161 tggggactag aaaggtgaag agccaaagtt agaactcagg accaacttat tctgattttg
     5221 tttttccaaa ctgcttctcc tcttgggaag tgtaaggaag ctgcagcacc aggatcagtg
     5281 aaacgcacca gacagccgcg tcagagcagc tcaggttctg ggagagggta gcgcagggtg
     5341 gccactgaga accgggcagg tcacgcatcc cccccttccc tcccaccccc tgccaagctc
     5401 tccctcccag gatcctctct ggctccatcg taagcaaacc ttagaggttc tggcaaggag
     5461 agagatggct ccaggaaatg ggggtgtgtc accagataag gaatctgcct aacaggaggt
     5521 gggggttaga cccaatatca ggagactagg aaggaggagg cctaaggatg gggcttttct
     5581 gtcaccaatc ctgtccctag taaagcttag tactgtcgat ccgctggcac gacaggtttc
     5641 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     5701 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     5761 aacaatttca cacaggaaac agctatgacc atgattacgc caagcttggg ctgcaggtcg
     5821 agggatctcc ataagagaag agggacagct atgactggga gtagtcagga gaggaggaaa
     5881 aatctggcta gtaaaacatg taaggaaaat tttagggatg ttaaagaaaa aaataacaca
     5941 aaacaaaata taaaaaaaat ctaacctcaa gtcaaggctt ttctatggaa taaggaatgg
     6001 acagcagggg gctgtttcat atactgatga cctctttata gccaaccttt gttcatggca
     6061 gccagcatat gggcatatgt tgccaaactc taaaccaaat actcattctg atgttttaaa
     6121 tgatttgccc tcccatatgt ccttccgagt gagagacaca aaaaattcca acacactatt
     6181 gcaatgaaaa taaatttcct ttattagcca gaagtcagat gctcaagggg cttcatgatg
     6241 tccccataat ttttggcaga gggaaaaaga tctcagtggt atttgtgagc cagggcattg
     6301 gccacaccag ccaccacctt ctgataggca gcctgcacct gaggagtgaa ttcacgcgtt
     6361 tacttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg
     6421 accatgtgat cgcgcttctc gttggggtct ttgctcagct tggactgggt gctcaggtag
     6481 tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg
     6541 gcgagctgca cgctgccgtc ctcgatgttg tggcggatct tgaagttcac cttgatgccg
     6601 ttcttctgct tgtcggccat gatatagacg ttgtggctgt tgtagttgta ctccagcttg
     6661 tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc
     6721 agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag
     6781 aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc
     6841 ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg
     6901 gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg
     6961 taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg
     7021 tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcaccatg
     7081 gtggcggaat tctttgccaa aatgatgaga cagcacaata accagcacgt tgcccaggag
     7141 ctgtaggaaa aagaagaagg catgaacatg gttagcagag gctctagagc cgccggtcac
     7201 acgccagaag ccgaaccccg ccctgccccg tcccccccga aggcagccgt ccccccgcgg
     7261 acagccccga ggctggagag ggagaagggg acggcggcgc ggcgacgcac gaaggccctc
     7321 cccgcccatt tccttcctgc cggcgccgca ccgcttcgcc ccgcgcccgc tagagggggt
     7381 gcggcggcgc ctcccagatt tcggctccgc acagatttgg gacaaaggaa gtccctgcgc
     7441 cctctcgcac gattaccata aaaggcaatg gctgcggctc gccgcgcctc gacagccgcc
     7501 ggcgctccgg gggccgccgc gcccctcccc cgagcccycc ccggcccgag gcggccccgc
     7561 cccgcccggc acccccacct gccgccaccc cccgcccggc acggcgagcc ccgcgccacg
     7621 ccccgtacgg agccccgcac ccgaagccgg gccgtgctca gcaactcggg gaggggggtg
     7681 cagggggggt tgcagcccga ccgacgcgcc cacaccccct gctcaccccc ccacgcacac
     7741 accccgcacg cagcctttgt tcccctcgca gccccccccg caccgcgggg caccgccccc
     7801 ggccgcgctc ccctcgcgca cactgcggag cgcacaaagc cccgcgccgc gcccgcagcg
     7861 ctcacagccg ccgggcagcg cggagccgca cgcggcgctc cccacgcaca cacacacgca
     7921 cgcacccccc gagccgctcc ccccgcacaa agggccctcc cggagcccct caaggctttc
     7981 acgcagccac agaaaagaaa caagccgtca ttaaaccaag cgctaattac agcccggagg
     8041 agaagggccg tcccgcccgc tcacctgtgg gagtaacgcg gtcagtcaga gccggggcgg
     8101 gcggcgcgag gcggcggcgg agcggggcac ggggcgaagg cagcgcgcag cgactcccgc
     8161 ccgccgcgcg cttcgctttt tatagggccg ccgccgccgc cgcctcgcca taaaaggaaa
     8221 ctttcggagc gcgccgctct gattggctgc cgccgcacct ctccgccycs ccccsccccs
     8281 ccccccsccc cccccccccc ccccyggcgc gcsccccccc cccccccgcc cccatcgctg
     8341 cacaaaataa ttaaaaaata aataaataca aaattggggg tggggagggg ggggagatgg
     8401 ggagagtgaa gcagaacgtg gggctcacct cgaccatggt aatagcgatg actaatacgt
     8461 agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa tgccaggcgg
     8521 gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac acttgatgta
     8581 ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg aaagtcccta
     8641 ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg
     8701 gtcagccagg cgggccattt accgtaagtt atgtaacgcg gaactccata tatgggctat
     8761 gaactaatga ccccgtaatt gattactatt aataactagt caataatcaa tgtcgagacc
     8821 catagagccc accgcatccc cagcatgcct gctattgtct tcccaatcct cccccttgct
     8881 gtcctgcccc accccacccc ccagaataga atgacaccta ctcagacaat gcgatgcaat
     8941 ttcctcattt tattaggaaa ggacagtggg agtggcacct tccagggtca aggaaggcac
     9001 gggggagggg caaacaacag atggctggca actagaaggc acagtcgagg ctgatcagcg
     9061 ggtttaaacg ggccctctag atcaggcacc gggcttgcgg gtcatgcacc aggtgcgcgg
     9121 tccttcgggc acctcgacgt cggcggtgac ggtgaagccg agccgctcgt agaaggggag
     9181 gttgcggggc gcggaggtct ccaggaaggc gggcaccccg gcgcgctcgg ccgcctccac
     9241 tccggggagc acgacggcgc tgcccagacc cttgccctgg tggtcgggcg agacgccgac
     9301 ggtggccagg aaccacgcgg gctccttggg ccggtgcggc gccaggaggc cttccatctg
     9361 ttgctgcgcg gccagccggg aaccgctcaa ctcggccatg cgcgggccga tctcggcgaa
     9421 caccgccccc gcttcgacgc tctccggcgt ggtccagacc gccaccgcgg cgccgtcgtc
     9481 cgcgacccac accttgccga tgtcgagccc gacgcgcgtg aggaagagtt cttgcagctc
     9541 ggtgacccgc tcgatgtggc ggtccggatc gacggtgtgg cgcgtggcgg ggtagtcggc
     9601 gaacgcggcg gcgagggtgc gtacggccct ggggacgtcg tcgcgggtgg cgaggcgcac
     9661 cgtgggcttg tactcggtca tctcgagcct agggccggga ttctcctcca cgtcaccgca
     9721 tgttagaaga cttcctctgc cctctccgct gccagatctc tcgaggccct gtgggaggaa
     9781 gagaagaggt cagaagctt
//
