LOCUS       sequence-456464-        8198 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8198)
  AUTHORS   Gottardi CJ, Wong E, Gumbiner BM
  TITLE     E-cadherin suppresses cellular transformation by inhibiting 
            beta-catenin signaling in an adhesion-independent manner.
  JOURNAL   J Cell Biol. 2001 May 28;153(5):1049-60.
  PUBMED    11381089
REFERENCE   2  (bases 1 to 8198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2001-05-28"; volume: "153(5)"; pages: "1049-60"
COMMENT     Sequence Label: hE-cadherin/Delta-beta-catenin-pcDNA3
FEATURES             Location/Qualifiers
     source          1..8198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3751..3769)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3752..3769)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3789..3806)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3795..4019
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4065..4493
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4152..4171)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4362..4383
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4502..4522)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4507..4836
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4687..4822
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4749..4768
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4903..5697
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4957..4976)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5567..5586
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5871..5992
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5908..5927)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5962..5981
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6041..6057)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6041..6057)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6054..6076)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6065..6081
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6089..6095,6096..6113,6114..6119))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6134..6155
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6272..6289)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6443..7031)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6523..6542)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7202..7993,7994..8062))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7825..7844
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8063..8167)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttctgcagat atcgaattcc
      961 ggaaagcacc tgtgagcttg cggaagtcag ttcagactcc agcccgctcc agcccggccc
     1021 gacccgaccg cacccggcgc ctgccctcgc tcggcgtccc cggccagcca tgggcccttg
     1081 gagccgcagc ctctcggcgc tgctgctgct gctgcaggtc tcctcttggc tctgccagga
     1141 gccggagccc tgccaccctg gctttgacgc cgagagctac acgttcacgg tgccccggcg
     1201 ccacctggag agaggccgcg tcctgggcag agtgaatttt gaagattgca ccggtcgaca
     1261 aaggacagcc tatttttccc tcgacacccg attcaaagtg ggcacagatg gtgtgattac
     1321 agtcaaaagg cctctacggt ttcataaccc acagatccat ttcttggtct acgcctggga
     1381 ctccacctac agaaagtttt ccaccaaagt cacgctgaat acagtggggc accaccaccg
     1441 ccccccgccc catcaggcct ccgtttctgg aatccaagca gaattgctca catttcccaa
     1501 ctcctctcct ggcctcagaa gacagaagag agactgggtt attcctccca tcagctgccc
     1561 agaaaatgaa aaaggcccat ttcctaaaaa cctggttcag atcaaatcca acaaagacaa
     1621 agaaggcaag gttttctaca gcatcactgg ccaaggagct gacacacccc ctgttggtgt
     1681 ctttattatt gaaagagaaa caggatggct gaaggtgaca gagcctctgg atagagaacg
     1741 cattgccaca tacactctct tctctcacgc tgtgtcatcc aacgggaatg cagttgagga
     1801 tccaatggag attttgatca cggtaaccga tcagaatgac aacaagcccg aattcaccca
     1861 ggaggtcttt aaggggtctg tcatggaagg tgctcttcca ggaacctctg tgatggaggt
     1921 cacagccaca gacgcggacg atgatgtgaa cacctacaat gccgccatcg cttacaccat
     1981 cctcagccaa gatcctgagc tccctgacaa aaatatgttc accattaaca ggaacacagg
     2041 agtcatcagt gtggtcacca ctgggctgga ccgagagagt ttccctacgt ataccctggt
     2101 ggttcaagct gctgaccttc aaggtgaggg gttaagcaca acagcaacag ctgtgatcac
     2161 agtcactgac accaacgata atcctccgat cttcaatccc accacgtaca agggtcaggt
     2221 gcctgagaac gaggctaacg tcgtaatcac cacactgaaa gtgactgatg ctgatgcccc
     2281 caatacccca gcgtgggagg ctgtatacac catattgaat gatgatggtg gacaatttgt
     2341 cgtcaccaca aatccagtga acaacgatgg cattttgaaa acagcaaagg gcttggattt
     2401 tgaggccaag cagcagtaca ttctacacgt agcagtgacg aatgtggtac cttttgaggt
     2461 ctctctcacc acctccacag ccaccgtcac cgtggatgtg ctggatgtga atgaagcccc
     2521 catctttgtg cctcctgaaa agagagtgga agtgtccgag gactttggcg tgggccagga
     2581 aatcacatcc tacactgccc aggagccaga cacatttatg gaacagaaaa taacatatcg
     2641 gatttggaga gacactgcca actggctgga gattaatccg gacactggtg ccatttccac
     2701 tcgggctgag ctggacaggg aggattttga gcacgtgaag aacagcacgt acacagccct
     2761 aatcatagct acagacaatg gttctccagt tgctactgga acagggacac ttctgctgat
     2821 cctgtctgat gtgaatgaca acgcccccat accagaacct cgaactatat tcttctgtga
     2881 gaggaatcca aagcctcagg tcataaacat cattgatgca gaccttcctc ccaatacatc
     2941 tcccttcaca gcagaactaa cacacggggc gagtgccaac tggaccattc agtacaacga
     3001 cccaacccaa gaatctatca ttttgaagcc aaagatggcc ttagaggtgg gtgactacaa
     3061 aatcaatctc aagctcatgg ataaccagaa taaagaccaa gtgaccacct tagaggtcag
     3121 cgtgtgtgac tgtgaagggg ccgccggcgt ctgtaggaag gcacagcctg tcgaagcagg
     3181 attgcaaatt cctgccattc tggggattct tggaggaatt cttgctttgc taattctgat
     3241 tctgctgctc ttgctgtttc ttcggaggag agcggtggtc aaagagccct tactgccccc
     3301 agaggatgac acccgggaca acgtttatta ctatgatgaa gaaggaggcg gagaagagga
     3361 ccaggacttt gacttgagcc agctgcacag gggcctggac gctcggcctg aagtgactcg
     3421 taacgacgtt gcaccaaccc tcatgagtgt cccccggtat cttccccgcc ctgccaatcc
     3481 cgatgaaatt ggaaatttta ttgatgaaaa tctgaaagcg gctgatactg accccacagc
     3541 cccgccttat gattctctgc tcgtgtttga ctatgaagga agcggttccg aagctgctag
     3601 tctgagctcc acttagacgg cgaggacgtg gcgatggcgc atgccgacgc gctagacgat
     3661 ttcgatctgg acatgttggg ggacggggat tccccgggtc cgggatcgat ccgtcgacat
     3721 atgctcgagc atgcatctag agggccctat tctatagtgt cacctaaatg ctagagctcg
     3781 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     3841 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     3901 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     3961 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     4021 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     4081 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     4141 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     4201 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     4261 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     4321 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     4381 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     4441 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
     4501 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     4561 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     4621 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     4681 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     4741 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     4801 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
     4861 attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
     4921 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     4981 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     5041 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg
     5101 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     5161 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     5221 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     5281 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     5341 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     5401 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg
     5461 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     5521 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     5581 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     5641 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
     5701 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
     5761 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     5821 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     5881 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     5941 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     6001 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     6061 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     6121 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     6181 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     6241 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     6301 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     6361 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     6421 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     6481 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     6541 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     6601 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     6661 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     6721 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     6781 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     6841 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     6901 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     6961 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     7021 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     7081 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     7141 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     7201 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     7261 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     7321 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     7381 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     7441 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     7501 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     7561 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     7621 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     7681 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     7741 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     7801 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     7861 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     7921 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     7981 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     8041 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     8101 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     8161 ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
