LOCUS       sequence-456472-        7229 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7229)
  AUTHORS   Kashatus DF, Lim KH, Brady DC, Pershing NL, Cox AD, Counter CM
  TITLE     RALA and RALBP1 regulate mitochondrial fission at mitosis.
  JOURNAL   Nat Cell Biol. 2011 Aug 7;13(9):1108-15. doi: 10.1038/ncb2310.
  PUBMED    21822277
REFERENCE   2  (bases 1 to 7229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Cell Biol"; date: "2011-08-7- 7"; volume: "13"; issue: "9"; 
            pages: "1108-15"
COMMENT     Sequence Label: pBABE-puro-hDrp1(K38A)
FEATURES             Location/Qualifiers
     source          1..7229
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..477
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    542..741
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             942..1358
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1288..1310
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1326..1342
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(3559..3579)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3564..3893
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3744..3879
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3903..4502
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3903..3922)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4399..4419
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4723..5192
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5322..5339)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5493..6081)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5573..5592)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6241..7032,7033..7101))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6864..6883
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7102..7206)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
      541 caagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
      601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
      661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
      721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
      781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
      841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt
      901 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
      961 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
     1021 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
     1081 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
     1141 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
     1201 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
     1261 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
     1321 cctcccttta tccagccctc actccttctc taggcgccgg ccggatccac tagtccagtg
     1381 tggtggaatt ctaatggagg cgctaattcc tgtcataaac aagctccagg acgtcttcaa
     1441 cacggtgggc gccgacatca tccagctgcc tcaaatcgtc gtagtgggaa cgcagagcag
     1501 cggagcgagc tcagtgctag aaagcctggt ggggagggac ctgcttccca gaggtactgg
     1561 aattgtcacc cggagacctc tcattctgca actggtccat gtttcacaag aagataaacg
     1621 gaaaacaaca ggagaagaaa atggggtgga agcagaagaa tggggtaaat ttcttcacac
     1681 caaaaataag ctttacacgg attttgatga aattcgacaa gaaattgaaa atgaaacaga
     1741 aagaatttca ggaaataata agggagtaag ccctgaacca attcatctta agattttttc
     1801 acccaacgtt gtcaatttga cacttgtgga tttgccagga atgaccaagg tgcctgtagg
     1861 tgatcaacct aaggatattg agcttcaaat cagagagctc attcttcggt tcatcagtaa
     1921 tcctaattcc attatcctcg ctgtcactgc tgctaataca gatatggcaa catcagaggc
     1981 acttaaaatt tcaagagagg tagatccaga tggtcgcaga accctagctg taatcactaa
     2041 acttgatctc atggatgcgg gtactgatgc catggatgta ttgatgggaa gggttattcc
     2101 agtcaaactt ggaataattg gagtagttaa caggagccag ctagatatta acaacaagaa
     2161 gagtgtaact gattcaatcc gtgatgagta tgcttttctt caaaagaaat atccatctct
     2221 ggccaataga aatggaacaa agtatcttgc taggactcta aacaggttac tgatgcatca
     2281 catcagagat tgtttaccag agttgaaaac aagaataaat gttctagctg ctcagtatca
     2341 gtctcttcta aatagctacg gtgaacccgt ggatgataaa agtgctactt tactccaact
     2401 tattaccaaa tttgccacag aatattgtaa cactattgaa ggaactgcaa aatatattga
     2461 aacttcggag ctatgcggtg gtgctagaat ttgttatatt ttccatgaga cttttgggcg
     2521 aaccttagaa tctgttgatc cacttggtgg ccttaacact attgacattt tgactgccat
     2581 tagaaatgct actggtcctc gtcctgcttt atttgtgcct gaggtttcat ttgagttact
     2641 ggtgaagcgg caaatcaaac gtctagaaga gcccagcctc cgctgtgtgg aactggttca
     2701 tgaggaaatg caaaggatca ttcagcactg tagcaattac agtacacagg aattgttacg
     2761 atttcctaaa cttcatgatg ccatagttga agtggtgact tgtcttcttc gtaaaaggtt
     2821 gcctgttaca aatgaaatgg tccataactt agtggcaatt gaactggctt atatcaacac
     2881 aaaacatcca gactttgctg atgcttgtgg gctaatgaac aataatatag aggaacaaag
     2941 gagaaacagg ctagccagag aattaccttc agctgtatca cgagacaagg ttgcatctgg
     3001 aggtggtggg gttggagatg gtgttcaaga accaaccaca ggcaactgga gaggaatgct
     3061 gaaaacttca aaagctgaag agttattagc agaagaaaaa tcaaaaccca ttccaattat
     3121 gccagccagt ccacaaaaag gtcatgccgt gaacctgcta gatgtgccag ttcctgttgc
     3181 acgaaaacta tctgctcggg aacagcgaga ttgtgaggtt attgaacgac tcattaaatc
     3241 atattttctc attgtcagaa agaatattca agacagtgtg ccaaaggcag taatgcattt
     3301 tttggttaat catgtgaaag acactcttca gagtgagcta gtaggccagc tgtataaatc
     3361 atccttattg gatgatcttc tgacagaatc tgaggacatg gcacagcgca ggaaagaagc
     3421 agctgatatg ctaaaggcat tacaaggagc cagtcaaatt attgctgaaa tccgggagac
     3481 tcatctttgg tgactcgagc atatgagatc ccagtgtggt ggtacgtagg aattcgccag
     3541 cacagtggtc gaccctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     3601 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     3661 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     3721 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     3781 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     3841 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctta
     3901 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     3961 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     4021 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     4081 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     4141 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     4201 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     4261 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     4321 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     4381 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     4441 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     4501 gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatcga
     4561 taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta
     4621 ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga
     4681 gaatagagaa gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca
     4741 ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg
     4801 aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa
     4861 cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc
     4921 cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg
     4981 cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc
     5041 tcactcgggg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa
     5101 accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga
     5161 gtgattgact acccgtcagc gggggtcttt cacatgcagc atgtatcaaa attaatttgg
     5221 ttttttttct taagtattta cattaaatgg ccatagttgc attaatgaat cggccaacgc
     5281 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     5341 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     5401 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     5461 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     5521 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     5581 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     5641 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     5701 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     5761 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     5821 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     5881 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     5941 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     6001 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     6061 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     6121 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     6181 tagatccttt tgcggccgca aatcaatcta aagtatatat gagtaaactt ggtctgacag
     6241 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     6301 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     6361 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     6421 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     6481 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     6541 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     6601 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     6661 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     6721 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     6781 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     6841 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     6901 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     6961 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     7021 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     7081 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     7141 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     7201 tccgcgcaca tttccccgaa aagtgccac
//
