LOCUS       sequence-456480-       10484 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10484)
  AUTHORS   Martinez-Noel G, Galligan JT, Sowa ME, Arndt V, Overton TM, Harper 
            JW, Howley PM
  TITLE     Identification and proteomic analysis of distinct UBE3A/E6AP protein
            complexes.
  JOURNAL   Mol Cell Biol. 2012 May 29.
  PUBMED    22645313
REFERENCE   2  (bases 1 to 10484)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2012 May 29."
COMMENT     Sequence Label: p6896 PHAGE-P CMVT N-HA-hE6AP II wt
FEATURES             Location/Qualifiers
     source          1..10484
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    52..169
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        221..524
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        525..728
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     679..699
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    730..748
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    751..769
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     779..803
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      890..899
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             902..928
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    944..968
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3639..3663)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3682..4181
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3717..3740)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4114..4133
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4202..4801
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4202..4221)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4698..4718
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4868..5456
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4921..4941)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5339..5350)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5531..5764
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5883..5901)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5969..6073
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6074..6142,6143..6934)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6292..6311)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7105..7693
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7594..7613
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7847..7864
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8104..8125
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8140..8145,8146..8163,8164..8170)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8178..8194
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8183..8205
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8202..8218
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8202..8218
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8268..8287
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8479..9112
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9159..9284
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9781..10014
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10199..10243
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 acggatctcg acggtatcgc cgaattcaca aatggcagta ttcatccaca attttaaaag
       61 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
      121 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
      181 cagggacagc agagatccag tttggactag tggagttccg cgttacataa cttacggtaa
      241 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      301 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      361 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      421 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      481 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
      541 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
      601 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
      661 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
      721 gcagagctct ccctatcagt gatagagatc tccctatcag tgatagagat cgtcgacgag
      781 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
      841 gaagacaccg ggaccgatcc agcctccgga ctctagcgtt taaacgctag ccaccatggc
      901 gtacccatac gatgttccag attacgctgc tcgaggttat caaacaagtt tgtacaaaaa
      961 agcaggctcc gcggccgccc ccttcaccat ggagaagctg caccagtgtt attggaaatc
     1021 aggagaacct cagtctgacg acattgaagc tagccgaatg aagcgagcag ctgcaaagca
     1081 tctaatagaa cgctactacc accagttaac tgagggctgt ggaaatgaag cctgcacgaa
     1141 tgagttttgt gcttcctgtc caacttttct tcgtatggat aataatgcag cagctattaa
     1201 agccctcgag ctttataaga ttaatgcaaa actctgtgat cctcatccct ccaagaaagg
     1261 agcaagctca gcttaccttg agaactcgaa aggtgccccc aacaactcct gctctgagat
     1321 aaaaatgaac aagaaaggcg ctagaattga ttttaaagat gtgacttact taacagaaga
     1381 gaaggtatat gaaattcttg aattatgtag agaaagagag gattattccc ctttaatccg
     1441 tgttattgga agagtttttt ctagtgctga ggcattggta cagagcttcc ggaaagttaa
     1501 acaacacacc aaggaagaac tgaaatctct tcaagcaaaa gatgaagaca aagatgaaga
     1561 tgaaaaggaa aaagctgcat gttctgctgc tgctatggaa gaagactcag aagcatcttc
     1621 ctcaaggata ggtgatagct cacagggaga caacaatttg caaaaattag gccctgatga
     1681 tgtgtctgtg gatattgatg ccattagaag ggtctacacc agattgctct ctaatgaaaa
     1741 aattgaaact gcctttctca atgcacttgt atatttgtca cctaacgtgg aatgtgactt
     1801 gacgtatcac aatgtatact ctcgagatcc taattatctg aatttgttca ttatcgtaat
     1861 ggagaataga aatctccaca gtcctgaata tctggaaatg gctttgccat tattttgcaa
     1921 agcgatgagc aagctacccc ttgcagccca aggaaaactg atcagactgt ggtctaaata
     1981 caatgcagac cagattcgga gaatgatgga gacatttcag caacttatta cttataaagt
     2041 cataagcaat gaatttaaca gtcgaaatct agtgaatgat gatgatgcca ttgttgctgc
     2101 ttcgaagtgc ttgaaaatgg tttactatgc aaatgtagtg ggaggggaag tggacacaaa
     2161 tcacaatgaa gaagatgatg aagagcccat ccctgagtcc agcgagctga cacttcagga
     2221 acttttggga gaagaaagaa gaaacaagaa aggtcctcga gtggaccccc tggaaactga
     2281 acttggtgtt aaaaccctgg attgtcgaaa accacttatc ccttttgaag agtttattaa
     2341 tgaaccactg aatgaggttc tagaaatgga taaagattat acttttttca aagtagaaac
     2401 agagaacaaa ttctctttta tgacatgtcc ctttatattg aatgctgtca caaagaattt
     2461 gggattatat tatgacaata gaattcgcat gtacagtgaa cgaagaatca ctgttctcta
     2521 cagcttagtt caaggacagc agttgaatcc atatttgaga ctcaaagtta gacgtgacca
     2581 tatcatagat gatgcacttg tccggctaga gatgatcgct atggaaaatc ctgcagactt
     2641 gaagaagcag ttgtatgtgg aatttgaagg agaacaagga gttgatgagg gaggtgtttc
     2701 caaagaattt tttcagctgg ttgtggagga aatcttcaat ccagatattg gtatgttcac
     2761 atacgatgaa tctacaaaat tgttttggtt taatccatct tcttttgaaa ctgagggtca
     2821 gtttactctg attggcatag tactgggtct ggctatttac aataactgta tactggatgt
     2881 acattttccc atggttgtct acaggaagct aatggggaaa aaaggaactt ttcgtgactt
     2941 gggagactct cacccagttc tatatcagag tttaaaagat ttattggagt atgaagggaa
     3001 tgtggaagat gacatgatga tcactttcca gatatcacag acagatcttt ttggtaaccc
     3061 aatgatgtat gatctaaagg aaaatggtga taaaattcca attacaaatg aaaacaggaa
     3121 ggaatttgtc aatctttatt ctgactacat tctcaataaa tcagtagaaa aacagttcaa
     3181 ggcttttcgg agaggttttc atatggtgac caatgaatct cccttaaagt acttattcag
     3241 accagaagaa attgaattgc ttatatgtgg aagccggaat ctagatttcc aagcactaga
     3301 agaaactaca gaatatgacg gtggctatac cagggactct gttctgatta gggagttctg
     3361 ggaaatcgtt cattcattta cagatgaaca gaaaagactc ttcttgcagt ttacaacggg
     3421 cacagacaga gcacctgtgg gaggactagg aaaattaaag atgattatag ccaaaaatgg
     3481 cccagacaca gaaaggttac ctacatctca tacttgcttt aatgtgcttt tacttccgga
     3541 atactcaagc aaagaaaaac ttaaagagag attgttgaag gccatcacgt atgccaaagg
     3601 atttggcatg ctgtaaggat ccaagggtgg gcgcgccgac ccagctttct tgtacaaagt
     3661 ggttcgataa cgaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat
     3721 gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac
     3781 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
     3841 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
     3901 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
     3961 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
     4021 gggctcagag gctgggaagg ggkgggtccg ggggcgggct caggggcggg ctcaggggcg
     4081 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt
     4141 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac
     4201 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     4261 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     4321 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     4381 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     4441 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     4501 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     4561 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     4621 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     4681 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     4741 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     4801 acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atgcatcgat
     4861 agatcctaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     4921 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     4981 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     5041 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     5101 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     5161 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     5221 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     5281 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     5341 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     5401 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctga
     5461 gatcctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     5521 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     5581 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     5641 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     5701 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     5761 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     5821 tcagagagtg agaggcccgg gttaattaag gaaagggcta gatcattctt gaagacgaaa
     5881 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     5941 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     6001 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     6061 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     6121 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     6181 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     6241 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     6301 cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc
     6361 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     6421 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     6481 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     6541 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     6601 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     6661 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     6721 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     6781 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     6841 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     6901 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     6961 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     7021 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     7081 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     7141 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     7201 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     7261 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     7321 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     7381 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     7441 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     7501 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     7561 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     7621 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     7681 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     7741 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     7801 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     7861 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     7921 ttaatgcagc aagctcatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     7981 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     8041 aaaaagctcc ccgtggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     8101 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     8161 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacat
     8221 gattacgaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     8281 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca
     8341 tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact
     8401 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg
     8461 tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa gatatccttg
     8521 atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc
     8581 caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag
     8641 ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc
     8701 atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat
     8761 ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt
     8821 gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg
     8881 agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc
     8941 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     9001 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     9061 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     9121 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     9181 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     9241 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     9301 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     9361 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     9421 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     9481 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     9541 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     9601 gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat
     9661 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     9721 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     9781 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     9841 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     9901 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     9961 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
    10021 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
    10081 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
    10141 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
    10201 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
    10261 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
    10321 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
    10381 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
    10441 agaaggtgga gagagagaca gagacagatc cattcgatta gtga
//
