LOCUS       sequence-456511-       15517 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15517)
  AUTHORS   Morin PJ, Sparks AB, Korinek V, Barker N, Clevers H, Vogelstein B, 
            Kinzler KW
  TITLE     Activation of beta-catenin-Tcf signaling in colon cancer by 
            mutations in beta-catenin or APC.
  JOURNAL   Science. 1997 Mar 21. 275(5307):1787-90.
  PUBMED    9065402
REFERENCE   2  (bases 1 to 15517)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            1997 Mar 21. 275(5307):1787-90."
COMMENT     Sequence Label: pCMV-Neo-Bam APC 1-2644
FEATURES             Location/Qualifiers
     source          1..15517
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(25..44)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    69..116
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        409..788
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        789..992
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     943..963
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     989..1013
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          1104..1676
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(10847..10866)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    10912..10967
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(10966..10985)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(11325..11341)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11325..11341)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11338..11360)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11349..11365
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11373..11379,11380..11397,11398..11403))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11418..11439
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11556..11573)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11727..12315)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11807..11826)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12486..13277,13278..13346))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13109..13128
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13347..13451)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     13519..13537
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(13575..13597)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     13697..13716
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     13910..13932
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     13924..13941
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     13925..13941
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        14352..14497
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             14554..15348
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(14608..14627)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     15218..15237
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 ccccgggaga tgggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg
       61 cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa
      121 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg
      181 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
      241 agggctcgca gccaacgtcg gggcggcaag ccctgccata gccacgggcc ccgtgggtta
      301 gggacggcgg atcgcggccc tctagagagc ttggcccatt gcatacgttg tatccatatc
      361 ataatatgta catttatatt ggctcatgtc caacattacc gccatgttga cattgattat
      421 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
      481 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      541 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      601 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      661 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      721 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      781 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      841 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      901 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      961 gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga
     1021 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccggt
     1081 cgaccgatcc tgagaacttc agggtgagtt tggggaccct tgattgttct ttctttttcg
     1141 ctattgtaaa attcatgtta tatggagggg gcaaagtttt cagggtgttg tttagaatgg
     1201 gaagatgtcc cttgtatcac catggaccct catgataatt ttgtttcttt cactttctac
     1261 tctgttgaca accattgtct cctcttattt tcttttcatt ttctgtaact ttttcgttaa
     1321 actttagctt gcatttgtaa cgaattttta aattcacttt tgtttatttg tcagattgta
     1381 agtactttct ctaatcactt ttttttcaag gcaatcaggg tatattatat tgtacttcag
     1441 cacagtttta gagaacaatt gttataatta aatgataagg tagaatattt ctgcatataa
     1501 attctggctg gcgtggaaat attcttattg gtagaaacaa ctacaccctg gtcatcatcc
     1561 tgcctttctc tttatggtta caatgatata cactgtttga gatgaggata aaatactctg
     1621 agtccaaacc gggcccctct gctaaccatg ttcatgcctt cttctctttc ctacagctcc
     1681 tgggcaacgt gctggttgtt gtgctgtctc atcattttgg caaagaattc ctcgacggat
     1741 ccgatcgaat tcccggaaat ggggtccaag ggtagccaag gatggctgca gcttcatatg
     1801 atcagttgtt aaagcaagtt gaggcactga agatggagaa ctcaaatctt cgacaagagc
     1861 tagaagataa ttccaatcat cttacaaaac tggaaactga ggcatctaat atgaaggaag
     1921 tacttaaaca actacaagga agtattgaag atgaagctat ggcttcttct ggacagattg
     1981 atttattaga gcgtcttaaa gagcttaact tagatagcag taatttccct ggagtaaaac
     2041 tgcggtcaaa aatgtccctc cgttcttatg gaagccggga aggatctgta tcaagccgtt
     2101 ctggagagtg cagtcctgtt cctatgggtt catttccaag aagagggttt gtaaatggaa
     2161 gcagagaaag tactggatat ttagaagaac ttgagaaaga gaggtcattg cttcttgctg
     2221 atcttgacaa agaagaaaag gaaaaagact ggtattacgc tcaacttcag aatctcacta
     2281 aaagaataga tagtcttcct ttaactgaaa atttttcctt acaaacagat atgaccagaa
     2341 ggcaattgga atatgaagca aggcaaatca gagttgcgat ggaagaacaa ctaggtacct
     2401 gccaggatat ggaaaaacga gcacagcgaa gaatagccag aattcagcaa atcgaaaagg
     2461 acatacttcg tatacgacag cttttacagt cccaagcaac agaagcagag aggtcatctc
     2521 agaacaagca tgaaaccggc tcacatgatg ctgagcggca gaatgaaggt caaggagtgg
     2581 gagaaatcaa catggcaact tctggtaatg gtcagggttc aactacacga atggaccatg
     2641 aaacagccag tgttttgagt tctagtagca cacactctgc acctcgaagg ctgacaagtc
     2701 atctgggaac caaggtggaa atggtgtatt cattgttgtc aatgcttggt actcatgata
     2761 aggatgatat gtcgcgaact ttgctagcta tgtctagctc ccaagacagc tgtatatcca
     2821 tgcgacagtc tggatgtctt cctctcctca tccagctttt acatggcaat gacaaagact
     2881 ctgtattgtt gggaaattcc cggggcagta aagaggctcg ggccagggcc agtgcagcac
     2941 tccacaacat cattcactca cagcctgatg acaagagagg caggcgtgaa atccgagtcc
     3001 ttcatctttt ggaacagata cgcgcttact gtgaaacctg ttgggagtgg caggaagctc
     3061 atgaaccagg catggaccag gacaaaaatc caatgccagc tcctgttgaa catcagatct
     3121 gtcctgctgt gtgtgttcta atgaaacttt catttgatga agagcataga catgcaatga
     3181 atgaactagg gggactacag gccattgcag aattattgca agtggactgt gaaatgtatg
     3241 ggcttactaa tgaccactac agtattacac taagacgata tgctggaatg gctttgacaa
     3301 acttgacttt tggagatgta gccaacaagg ctacgctatg ctctatgaaa ggctgcatga
     3361 gagcacttgt ggcccaacta aaatctgaaa gtgaagactt acagcaggtt attgcgagtg
     3421 ttttgaggaa tttgtcttgg cgagcagatg taaatagtaa aaagacgttg cgagaagttg
     3481 gaagtgtgaa agcattgatg gaatgtgctt tagaagttaa aaaggaatca accctcaaaa
     3541 gcgtattgag tgccttatgg aatttgtcag cacattgcac tgagaataaa gctgatatat
     3601 gtgctgtaga tggtgcactt gcatttttgg ttggcactct tacttaccgg agccagacaa
     3661 acactttagc cattattgaa agtggaggtg ggatattacg gaatgtgtcc agcttgatag
     3721 ctacaaatga ggaccacagg caaatcctaa gagagaacaa ctgtctacaa actttattac
     3781 aacacttaaa atctcatagt ttgacaatag tcagtaatgc atgtggaact ttgtggaatc
     3841 tctcagcaag aaatcctaaa gaccaggaag cattatggga catgggggca gttagcatgc
     3901 tcaagaacct cattcattca aagcacaaaa tgattgctat gggaagtgct gcagctttaa
     3961 ggaatctcat ggcaaatagg cctgcgaagt acaaggatgc caatattatg tctcctggct
     4021 caagcttgcc atctcttcat gttaggaaac aaaaagccct agaagcagaa ttagatgctc
     4081 agcacttatc agaaactttt gacaatatag acaatttaag tcccaaggca tctcatcgta
     4141 gtaagcagag acacaagcaa agtctctatg gtgattatgt ttttgacacc aatcgacatg
     4201 atgataatag gtcagacaat tttaatactg gcaacatgac tgtcctttca ccatatttga
     4261 atactacagt gttacccagc tcctcttcat caagaggaag cttagatagt tctcgttctg
     4321 aaaaagatag aagtttggag agagaacgcg gaattggtct aggcaactac catccagcaa
     4381 cagaaaatcc aggaacttct tcaaagcgag gtttgcagat ctccaccact gcagcccaga
     4441 ttgccaaagt catggaagaa gtgtcagcca ttcatacctc tcaggaagac agaagttctg
     4501 ggtctaccac tgaattacat tgtgtgacag atgagagaaa tgcacttaga agaagctctg
     4561 ctgcccatac acattcaaac acttacaatt tcactaagtc ggaaaattca aataggacat
     4621 gttctatgcc ttatgccaaa ttagaataca agagatcttc aaatgatagt ttaaatagtg
     4681 tcagtagtag tgatggttat ggtaaaagag gtcaaatgaa accctcgatt gaatcctatt
     4741 ctgaagatga tgaaagtaag ttttgcagtt atggtcaata cccagccgac ctagcccata
     4801 aaatacatag tgcaaatcat atggatgata atgatggaga actagataca ccaataaatt
     4861 atagtcttaa atattcagat gagcagttga actctggaag gcaaagtcct tcacagaatg
     4921 aaagatgggc aagacccaaa cacataatag aagatgaaat aaaacaaagt gagcaaagac
     4981 aatcaaggaa tcaaagtaca acttatcctg tttatactga gagcactgat gataaacacc
     5041 tcaagttcca accacatttt ggacagcagg aatgtgtttc tccatacagg tcacggggag
     5101 ccaatggttc agaaacaaat cgagtgggtt ctaatcatgg aattaatcaa aatgtaagcc
     5161 agtctttgtg tcaagaagat gactatgaag atgataagcc taccaattat agtgaacgtt
     5221 actctgaaga agaacagcat gaagaagaag agagaccaac aaattatagc ataaaatata
     5281 atgaagagaa acgtcatgtg gatcagccta ttgattatag tttaaaatat gccacagata
     5341 ttccttcatc acagaaacag tcattttcat tctcaaagag ttcatctgga caaagcagta
     5401 aaaccgaaca tatgtcttca agcagtgaga atacgtccac accttcatct aatgccaaga
     5461 ggcagaatca gctccatcca agttctgcac agagtagaag tggtcagcct caaaaggctg
     5521 ccacttgcaa agtttcttct attaaccaag aaacaataca gacttattgt gtagaagata
     5581 ctccaatatg tttttcaaga tgtagttcat tatcatcttt gtcatcagct gaagatgaaa
     5641 taggatgtaa tcagacgaca caggaagcag attctgctaa taccctgcaa atagcagaaa
     5701 taaaagaaaa gattggaact aggtcagctg aagatcctgt gagcgaagtt ccagcagtgt
     5761 cacagcaccc tagaaccaaa tccagcagac tgcagggttc tagtttatct tcagaatcag
     5821 ccaggcacaa agctgttgaa ttttcttcag gagcgaaatc tccctccaaa agtggtgctc
     5881 agacacccaa aagtccacct gaacactatg ttcaggagac cccactcatg tttagcagat
     5941 gtacttctgt cagttcactt gatagttttg agagtcgttc gattgccagc tccgttcaga
     6001 gtgaaccatg cagtggaatg gtaagtggca ttataagccc cagtgatctt ccagatagcc
     6061 ctggacaaac catgccacca agcagaagta aaacacctcc accacctcct caaacagctc
     6121 aaaccaagcg agaagtacct aaaaataaag cacctactgc tgaaaagaga gagagtggac
     6181 ctaagcaagc tgcagtaaat gctgcagttc agagggtcca ggttcttcca gatgctgata
     6241 ctttattaca ttttgccaca gaaagtactc cagatggatt ttcttgttca tccagcctga
     6301 gtgctctgag cctcgatgag ccatttatac agaaagatgt ggaattaaga ataatgcctc
     6361 cagttcagga aaatgacaat gggaatgaaa cagaatcaga gcagcctaaa gaatcaaatg
     6421 aaaaccaaga gaaagaggca gaaaaaacta ttgattctga aaaggaccta ttagatgatt
     6481 cagatgatga tgatattgaa atactagaag aatgtattat ttctgccatg ccaacaaagt
     6541 catcacgtaa agcaaaaaag ccagcccaga ctgcttcaaa attacctcca cctgtggcaa
     6601 ggaaaccaag tcagctgcct gtgtacaaac ttctaccatc acaaaacagg ttgcaacccc
     6661 aaaagcatgt tagttttaca ccgggggatg atatgccacg ggtgtattgt gttgaaggga
     6721 cacctataaa cttttccaca gctacatctc taagtgatct aacaatcgaa tcccctccaa
     6781 atgagttagc tgctggagaa ggagttagag gaggagcaca gtcaggtgaa tttgaaaaac
     6841 gagataccat tcctacagaa ggcagaagta cagatgaggc tcaaggagga aaaacctcat
     6901 ctgtaaccat acctgaattg gatgacaata aagcagagga aggtgatatt cttgcagaat
     6961 gcattaattc tgctatgccc aaagggaaaa gtcacaagcc tttccgtgtg aaaaagataa
     7021 tggaccaggt ccagcaagca tctgcgtcgt cttctgcacc caacaaaaat cagttagatg
     7081 gtaagaaaaa gaaaccaact tcaccagtaa aacctatacc acaaaatact gaatatagga
     7141 cacgtgtaag aaaaaatgca gactcaaaaa ataatttaaa tgctgagaga gttttctcag
     7201 acaacaaaga ttcaaagaaa cagaatttga aaaataattc caaggacttc aatgataagc
     7261 tcccaaataa tgaagataga gtcagaggaa gttttgcttt tgattcacct catcattaca
     7321 cgcctattga aggaactcct tactgttttt cacgaaatga ttctttgagt tctctagatt
     7381 ttgatgatga tgatgttgac ctttccaggg aaaaggctga attaagaaag gcaaaagaaa
     7441 ataaggaatc agaggctaaa gttaccagcc acacagaact aacctccaac caacaatcag
     7501 ctaataagac acaagctatt gcaaagcagc caataaatcg aggtcagcct aaacccatac
     7561 ttcagaaaca atccactttt ccccagtcat ccaaagacat accagacaga ggggcagcaa
     7621 ctgatgaaaa gttacagaat tttgctattg aaaatactcc agtttgcttt tctcataatt
     7681 cctctctgag ttctctcagt gacattgacc aagaaaacaa caataaagaa aatgaaccta
     7741 tcaaagagac tgagccccct gactcacagg gagaaccaag taaacctcaa gcatcaggct
     7801 atgctcctaa atcatttcat gttgaagata ccccagtttg tttctcaaga aacagttctc
     7861 tcagttctct tagtattgac tctgaagatg acctgttgca ggaatgtata agctccgcaa
     7921 tgccaaaaaa gaaaaagcct tcaagactca agggtgataa tgaaaaacat agtcccagaa
     7981 atatgggtgg catattaggt gaagatctga cacttgattt gaaagatata cagagaccag
     8041 attcagaaca tggtctatcc cctgattcag aaaattttga ttggaaagct attcaggaag
     8101 gtgcaaattc catagtaagt agtttacatc aagctgctgc tgctgcatgt ttatctagac
     8161 aagcttcgtc tgattcagat tccatccttt ccctgaaatc aggaatctct ctgggatcac
     8221 catttcatct tacacctgat caagaagaaa aaccctttac aagtaataaa ggcccacgaa
     8281 ttctaaaacc aggggagaaa agtacattgg aaactaaaaa gatagaatct gaaagtaaag
     8341 gaatcaaagg aggaaaaaaa gtttataaaa gtttgattac tggaaaagtt cgatctaatt
     8401 cagaaatttc aggccaaatg aaacagcccc ttcaagcaaa catgccttca atctctcgag
     8461 gcaggacaat gattcatatt ccaggagttc gaaatagctc ctcaagtaca agtcctgttt
     8521 ctaaaaaagg cccacccctt aagactccag cctccaaaag ccctagtgaa ggtcaaacag
     8581 ccaccacttc tcctagagga gccaagccat ctgtgaaatc agaattaagc cctgttgcca
     8641 ggcagacatc ccaaataggt gggtcaagta aagcaccttc tagatcagga tctagagatt
     8701 cgaccccttc aagacctgcc cagcaaccat taagtagacc tatacagtct cctggccgaa
     8761 actcaatttc ccctggtaga aatggaataa gtcctcctaa caaattatct caacttccaa
     8821 ggacatcatc ccctagtact gcttcaacta agtcctcagg ttctggaaaa atgtcatata
     8881 catctccagg tagacagatg agccaacaga accttaccaa acaaacaggt ttatccaaga
     8941 atgccagtag tattccaaga agtgagtctg cctccaaagg actaaatcag atgaataatg
     9001 gtaatggagc caataaaaag gtagaacttt ctagaatgtc ttcaactaaa tcaagtggaa
     9061 gtgaatctga tagatcagaa agacctgtat tagtacgcca gtcaactttc atcaaagaag
     9121 ctccaagccc aaccttaaga agaaaattgg aggaatctgc ttcatttgaa tctctttctc
     9181 catcatctag accagcttct cccactaggt cccaggcaca aactccagtt ttaagtcctt
     9241 cccttcctga tatgtctcta tccacacatt cgtctgttca ggctggtgga tggcgaaaac
     9301 tcccacctaa tctcagtccc actatagagt ataatgatgg aagaccagca aagcgccatg
     9361 atattgcacg gtctcattct gaaagtcctt ctagacttcc aatcaatagg tcaggaacct
     9421 ggaaacgtga gcacagcaaa cattcatcat cccttcctcg agtaagcact tggagaagaa
     9481 ctggaagttc atcttcaatt ctttctgctt catcagaatc cagtgaaaaa gcaaaaagtg
     9541 aggatgaaaa acatgtgaac tctatttcag gaaccaaaca aagtaaagaa aaccaagtat
     9601 ccgcaaaagg aacatggaga aaaataaaag aaaatgaatt ttctcccaca aatagtactt
     9661 ctcagaccgt ttcctcaggt gctacaaatg gtgctgaatc aaagactcta atcaaatggc
     9721 acctgctgtt tctaaaacag aggatgtttg ggtgagaatt gaggactgtc ccattaacaa
     9781 tcctagatct ggaagatctc ccacaggtaa tactcccccg gtgattgaca gtgtttcaga
     9841 aaaggcaaat ccaaacatta aagattcaaa agataatcag gcaaaacaaa atgtgggtaa
     9901 tggcagtgtt cccatgcgta ccgtgggttt ggaaaatcgc ctgaactcct ttattcaggt
     9961 ggatgcccct gaccaaaaag gaactgagat aaaaccagga caaaataatc ctgtccctgt
    10021 atcagagact aatgaaagtt ctatagtgga acgtacccca ttcagttcta gcagctcaag
    10081 caaacacagt tcacctagtg ggactgttgc tgccagagtg actcctttta attacaaccc
    10141 aagccctagg aaaagcagcg cagatagcac ttcagctcgg ccatctcaga tcccaactcc
    10201 agtgaataac aacacaaaga agcgagattc caaaactgac agcacagaat ccagtggaac
    10261 ccaaagtcct aagcgccatt ctgggtctta ccttgtgaca tctgtttaaa agagaggaag
    10321 aatgaaacta agaaaattct atgttaatta caactgctat atagacattt tgtttcaaat
    10381 gaaactttaa aagactgaaa aattttgtaa ataggtttga ttcttgttag agggtttttg
    10441 ttctcgaagc catatttgat agtatacttt gtcttcactg gtcttatttt gggaggcact
    10501 cttgatggtt aggaaaaaaa tagtaaagcc aagtatgttt gtacagtatg ttttacatgt
    10561 atttaaagta gcatcccatc ccaacttcct ttaattattg cttgtcttaa aataatgaac
    10621 actacagata gaaaatatga tatattgctg ttatcaatca tttctagatt ataaactgac
    10681 taaacttaca tcagggaaaa attggtattt atgcaaaaaa aaatgttttt gtggaattcc
    10741 tgcagcccgg gggatccgtc gaggaattca ctcctcaggt gcaggctgcc tatcagaagg
    10801 tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg
    10861 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga
    10921 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
    10981 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat
    11041 atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga
    11101 aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt
    11161 tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg
    11221 ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc
    11281 tcttatggag atccctcgac ggatcggccg caattcgtaa tcatgtcata gctgtttcct
    11341 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
    11401 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
    11461 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
    11521 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
    11581 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
    11641 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
    11701 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
    11761 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    11821 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    11881 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    11941 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    12001 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    12061 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    12121 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
    12181 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    12241 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    12301 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    12361 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    12421 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    12481 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    12541 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    12601 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    12661 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    12721 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    12781 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    12841 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    12901 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    12961 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    13021 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    13081 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    13141 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    13201 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    13261 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    13321 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    13381 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    13441 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
    13501 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
    13561 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
    13621 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
    13681 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
    13741 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg
    13801 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
    13861 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
    13921 cgttgtaaaa cgacggccag tgccaagctt cacgctgccg caagcactca gggcgcaagg
    13981 gctgctaaag gaagcggaac acgtagaaag ccagtccgca gaaacggtgc tgaccccgga
    14041 tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag agaaagcagg
    14101 tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg
    14161 aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact
    14221 ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaagatcc tgcttcatcc
    14281 ccgtggcccg ttgctcgcgt ttgctggcgg tgtccccgga agaaatatat ttgcatgtct
    14341 ttagttctat gatgacacaa accccgccca gcgtcttgtc attggcgaat tcgaacacgc
    14401 agatgcagtc ggggcggcgc ggtcccaggt ccacttcgca tattaaggtg acgcgtgtgg
    14461 cctcgaacac cgagcgaccc tgcagcgacc cgcttaacag cgtcaacagc gtgccgcaga
    14521 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
    14581 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
    14641 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
    14701 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
    14761 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
    14821 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
    14881 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
    14941 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
    15001 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
    15061 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
    15121 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
    15181 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
    15241 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
    15301 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
    15361 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
    15421 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
    15481 tccagcgcgg ggatctcatg ctggagttct tcgccca
//
