LOCUS       sequence-456514-        9535 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9535)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 9535)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: AR-V7-pcw107
FEATURES             Location/Qualifiers
     source          1..9535
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        15..515
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     434..452
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    536..560
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2511..2535)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2567..3155
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2620..2640)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3038..3049)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3227..3460
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3538..3659
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3575..3594)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3629..3648
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3699..3834
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3854..3873)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3855..3873)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3883..3900)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3883..3899)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3892..3914)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4041..4496
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4128..4147)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4338..4359
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4522..4626
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4627..4695,4696..5487)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4845..4864)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5658..6246
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6147..6166
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6400..6417
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6534..6555
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6570..6575,6576..6593,6594..6600)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6608..6624
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6613..6635
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6632..6648
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6632..6648
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6667..6687
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6669..6687
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6715..6941
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6942..7122
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7169..7294
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7787..8020
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8205..8249
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8480..8690
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8502..8698
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8549..8684
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8730..9332
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8730..8749)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9226..9246
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9375..9492
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tcgaggcctg caggttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct
       61 gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc acattcttca
      121 cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc
      181 ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac
      241 aaacggaagc cgcacgtctc actagtaccc tcgcagacgg acagcgccag ggagcaatgg
      301 cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg cgcgccgaga
      361 gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt
      421 tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg gcagtcggct
      481 ccctcgttga ccgaatcacc gacctctctc cccaggggct agcatcgatg gatcaacaag
      541 tttgtacaaa aaagttggcg ctggcggcgg cggcaccatg gaagtgcagt tagggctggg
      601 aagggtctac cctcggccgc cgtccaagac ctaccgagga gctttccaga atctgttcca
      661 gagcgtgcgc gaagtgatcc agaacccggg ccccaggcac ccagaggccg cgagcgcagc
      721 acctcccggc gccagtttgc tgctgcagca gcagcagcag cagcagcagc agcagcagca
      781 gcagcagcag cagcagcagc agcagcagca gcagcagcag cagcaagaga ctagccccag
      841 gcagcagcag cagcagcagg gtgaggatgg ttctccccaa gcccatcgta gaggccccac
      901 aggctacctg gtcctggatg aggaacagca accttcacag ccgcagtcgg ccctggagtg
      961 ccaccccgag agaggttgcg tcccagagcc tggagccgcc gtggccgcca gcaaggggct
     1021 gccgcagcag ctgccagcac ctccggacga ggatgactca gctgccccat ccacgttgtc
     1081 cctgctgggc cccactttcc ccggcttaag cagctgctcc gctgacctta aagacatcct
     1141 gagcgaggcc agcaccatgc aactccttca gcaacagcag caggaagcag tatccgaagg
     1201 cagcagcagc gggagagcga gggaggcctc gggggctccc acttcctcca aggacaatta
     1261 cttagggggc acttcgacca tttctgacaa cgccaaggag ttgtgtaagg cagtgtcggt
     1321 gtccatgggc ctgggtgtgg aggcgttgga gcatctgagt ccaggggaac agcttcgggg
     1381 ggattgcatg tacgccccac ttttgggagt tccacccgct gtgcgtccca ctccttgtgc
     1441 cccattggcc gaatgcaaag gttctctgct agacgacagc gcaggcaaga gcactgaaga
     1501 tactgctgag tattcccctt tcaagggagg ttacaccaaa gggctagaag gcgagagcct
     1561 aggctgctct ggcagcgctg cagcagggag ctccgggaca cttgaactgc cgtctaccct
     1621 gtctctctac aagtccggag cactggacga ggcagctgcg taccagagtc gcgactacta
     1681 caactttcca ctggctctgg ccggaccgcc gccccctccg ccgcctcccc atccccacgc
     1741 tcgcatcaag ctggagaacc cgctggacta cggcagcgcc tgggcggctg cggcggcgca
     1801 gtgccgctat ggggacctgg cgagcctgca tggcgcgggt gcagcgggac ccggttctgg
     1861 gtcaccctca gccgccgctt cctcatcctg gcacactctc ttcacagccg aagaaggcca
     1921 gttgtatgga ccgtgtggtg gtggtggggg tggtggcggc ggcggcggcg gcggcggcgg
     1981 cggcggcggc ggcgaggcgg gagctgtagc cccctacggc tacactcggc cccctcaggg
     2041 gctggcgggc caggaaagcg acttcaccgc acctgatgtg tggtaccctg gcggcatggt
     2101 gagcagagtg ccctatccca gtcccacttg tgtcaaaagc gaaatgggcc cctggatgga
     2161 tagctactcc ggaccttacg gggacatgcg tttggagact gccagggacc atgttttgcc
     2221 cattgactat tactttccac cccagaagac ctgcctgatc tgtggagatg aagcttctgg
     2281 gtgtcactat ggagctctca catgtggaag ctgcaaggtc ttcttcaaaa gagccgctga
     2341 agggaaacag aagtacctgt gcgccagcag aaatgattgc actattgata aattccgaag
     2401 gaaaaattgt ccatcttgtc gtcttcggaa atgttatgaa gcagggatga ctctgggaga
     2461 aaaattccgg gttggcaatt gcaagcatct caaaatgacc agaccctgat tgccaacttt
     2521 cttgtacaaa gtggttaccg gttagtaatg aacgcgttaa gtcgacaatc aacctctgga
     2581 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     2641 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     2701 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     2761 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     2821 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     2881 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     2941 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     3001 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     3061 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     3121 gacgagtcgg atctcccttt gggccgcctc cccgcgtcga ctttaagacc aatgacttac
     3181 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     3241 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     3301 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     3361 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     3421 tccctcagac ccttttagtc agtgtggaaa atctctagca gtacgtatag tagttcatgt
     3481 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
     3541 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     3601 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     3661 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca
     3721 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     3781 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga
     3841 cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
     3901 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     3961 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     4021 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     4081 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     4141 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     4201 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     4261 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     4321 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     4381 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     4441 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
     4501 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     4561 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     4621 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     4681 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     4741 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     4801 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     4861 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     4921 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     4981 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     5041 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     5101 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     5161 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     5221 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     5281 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     5341 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     5401 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     5461 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     5521 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     5581 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     5641 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     5701 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     5761 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     5821 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     5881 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     5941 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6001 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6061 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6121 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6181 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6241 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6301 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     6361 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     6421 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     6481 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     6541 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     6601 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     6661 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt
     6721 cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac
     6781 aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct
     6841 tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt
     6901 gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc
     6961 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     7021 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     7081 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     7141 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     7201 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     7261 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     7321 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     7381 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     7441 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     7501 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     7561 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     7621 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     7681 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     7741 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     7801 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     7861 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     7921 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     7981 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     8041 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     8101 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     8161 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     8221 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     8281 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     8341 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     8401 acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa ccaggtgtgg
     8461 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca attagtcagc
     8521 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     8581 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     8641 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     8701 gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg gtgcgcctcg
     8761 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
     8821 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag
     8881 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     8941 ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     9001 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     9061 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     9121 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     9181 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     9241 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     9301 ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag acaaatggca
     9361 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     9421 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     9481 attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg ccggc
//
