LOCUS       sequence-45652-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Dillin A, Crawford DK, Kenyon C
  TITLE     Timing requirements for insulin/IGF-1 signaling in C. elegans.
  JOURNAL   Science. 2002 Oct 25;298(5594):830-4.
  PUBMED    12399591
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2002-10-25"; volume: "298(5594)"; pages: "830-4"
COMMENT     Sequence Label: pAD12
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..27
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9..27
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(120..136)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(121..137)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(162..180)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(190..207)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(190..206)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(199..221)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      348..803
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(435..454)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     645..666
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctcgcgta atacgactca
       61 ctataggggc gaagcactag tggatccccc gggctgcagg aattcgatat caagcttatc
      121 gataccgtcg acctcgaggg ggggcccggt acccaattcg ccctatagtg agtcgtatta
      181 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
      241 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
      301 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
      361 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
      421 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
      481 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
      541 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
      601 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
      661 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
      721 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
      781 caaaatatta acgcttacaa tttaggtggc acttttcgg
//
