LOCUS       sequence-456522-       15522 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15522)
  AUTHORS   Morin PJ, Sparks AB, Korinek V, Barker N, Clevers H, Vogelstein B, 
            Kinzler KW
  TITLE     Activation of beta-catenin-Tcf signaling in colon cancer by 
            mutations in beta-catenin or APC.
  JOURNAL   Science. 1997 Mar 21. 275(5307):1787-90.
  PUBMED    9065402
REFERENCE   2  (bases 1 to 15522)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            1997 Mar 21. 275(5307):1787-90."
COMMENT     Sequence Label: pCMV-Neo-Bam APC 1-1941
FEATURES             Location/Qualifiers
     source          1..15522
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(5364..5383)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5429..5484
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5483..5502)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5842..5858)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5842..5858)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5855..5877)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5866..5882
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5890..5896,5897..5914,5915..5920))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5935..5956
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6073..6090)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6244..6832)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6324..6343)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7003..7794,7795..7863))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7626..7645
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7864..7968)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8036..8054
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8092..8114)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8214..8233
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8427..8449
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8441..8458
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8442..8458
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8869..9014
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             9071..9865
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9125..9144)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9735..9754
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10059..10078)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10103..10150
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        10443..10822
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10823..11026
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10977..10997
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     11023..11047
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          11138..11710
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
ORIGIN
        1 acagagtaga agtggtcagc ctcaaaaggc tgccacttgc aaagtttctt ctattaacca
       61 agaaacaata cagacttatt gtgtagaaga tactccaata tgtttttcaa gatgtagttc
      121 attatcatct ttgtcatcag ctgaagatga aataggatgt aatcagacga cacaggaagc
      181 agattctgct aataccctgc aaatagcaga aataaaagaa aagattggaa ctaggtcagc
      241 tgaagatcct gtgagcgaag ttccagcagt gtcacagcac cctagaacca aatccagcag
      301 actgcagggt tctagtttat cttcagaatc agccaggcac aaagctgttg aattttcttc
      361 aggagcgaaa tctccctcca aaagtggtgc tcagacaccc aaaagtccac ctgaacacta
      421 tgttcaggag accccactca tgtttagcag atgtacttct gtcagttcac ttgatagttt
      481 tgagagtcgt tcgattgcca gctccgttca gagtgaacca tgcagtggaa tggtaagtgg
      541 cattataagc cccagtgatc ttccagatag ccctggacaa accatgccac caagcagaag
      601 taaaacacct ccaccacctc ctcaaacagc tcaaaccaag cgagaagtac ctaaaaataa
      661 agcacctact gctgaaaaga gagagagtgg acctaagcaa gctgcagtaa atgctgcagt
      721 tcagagggtc caggttcttc cagatgctga tactttatta cattttgcca cagaaagtac
      781 tccagatgga ttttcttgtt catccagcct gagtgctctg agcctcgatg agccatttat
      841 acagaaagat gtggaattaa gaataatgcc tccagttcag gaaaatgaca atgggaatga
      901 aacagaatca gagcagccta aagaatcaaa tgaaaaccaa gagaaagagg cagaaaaaac
      961 tattgattct gaaaaggacc tattagatga ttcagatgat gatgatattg aaatactaga
     1021 agaatgtatt atttctgcca tgccaacaaa gtcatcacgt aaagcaaaaa agccagccca
     1081 gactgcttca aaattacctc cacctgtggc aaggaaacca agtcagctgc ctgtgtacaa
     1141 acttctacca tcacaaaaca ggttgcaacc ccaaaagcat gttagtttta caccggggga
     1201 tgatatgcca cgggtgtatt gtgttgaagg gacacctata aacttttcca cagctacatc
     1261 tctaagtgat ctaacaatcg aatcccctcc aaatgagtta gctgctggag aaggagttag
     1321 aggaggagca cagtcaggtg aatttgaaaa acgagatacc attcctacag aaggcagaag
     1381 tacagatgag gctcaaggag gaaaaacctc atctgtaacc atacctgaat tggatgacaa
     1441 taaagcagag gaaggtgata ttcttgcaga atgcattaat tctgctatgc ccaaagggaa
     1501 aagtcacaag cctttccgtg tgaaaaagat aatggaccag gtccagcaag catctgcgtc
     1561 gtcttctgca cccaacaaaa atcagttaga tggtaagaaa aagaaaccaa cttcaccagt
     1621 aaaacctata ccacaaaata ctgaatatag gacacgtgta agaaaaaatg cagactcaaa
     1681 aaataattta aatgctgaga gagttttctc agacaacaaa gattcaaaga aacagaattt
     1741 gaaaaataat tccaaggact tcaatgataa gctcccaaat aatgaagata gagtcagagg
     1801 aagttttgct tttgattcac ctcatcatta cacgcctatt gaaggaactc cttactgttt
     1861 ttcacgaaat gattctttga gttctctaga ttttgatgat gatgatgttg acctttccag
     1921 ggaaaaggct gaattaagaa aggcaaaaga aaataaggaa tcagaggcta aagttaccag
     1981 ccacacagaa ctaacctcca accaacaatc agctaataag acacaagcta ttgcaaagca
     2041 gccaataaat cgaggtcagc ctaaacccat acttcagaaa caatccactt ttccccagtc
     2101 atccaaagac ataaccagac agaggggcag caactgatga aaagttacag aattttgcta
     2161 ttgaaaatac tccagtttgc ttttctcata attcctctct gagttctctc agtgacattg
     2221 accaagaaaa caacaataaa gaaaatgaac ctatcaaaga gactgagccc cctgactcac
     2281 agggagaacc aagtaaacct caagcatcag gctatgctcc taaatcattt catgttgaag
     2341 ataccccagt ttgtttctca agaaacagtt ctctcagttc tcttagtatt gactctgaag
     2401 atgacctgtt gcaggaatgt ataagctccg caatgccaaa aaagaaaaag ccttcaagac
     2461 tcaagggtga taatgaaaaa catagtccca gaaatatggg tggcatatta ggtgaagatc
     2521 tgacacttga tttgaaagat atacagagac cagattcaga acatggtcta tcccctgatt
     2581 cagaaaattt tgattggaaa gctattcagg aaggtgcaaa ttccatagta agtagtttac
     2641 atcaagctgc tgctgctgca tgtttatcta gacaagcttc gtctgattca gattccatcc
     2701 tttccctgaa atcaggaatc tctctgggat caccatttca tcttacacct gatcaagaag
     2761 aaaaaccctt tacaagtaat aaaggcccac gaattctaaa accaggggag aaaagtacat
     2821 tggaaactaa aaagatagaa tctgaaagta aaggaatcaa aggaggaaaa aaagtttata
     2881 aaagtttgat tactggaaaa gttcgatcta attcagaaat ttcaggccaa atgaaacagc
     2941 cccttcaagc aaacatgcct tcaatctctc gaggcaggac aatgattcat attccaggag
     3001 ttcgaaatag ctcctcaagt acaagtcctg tttctaaaaa aggcccaccc cttaagactc
     3061 cagcctccaa aagccctagt gaaggtcaaa cagccaccac ttctcctaga ggagccaagc
     3121 catctgtgaa atcagaatta agccctgttg ccaggcagac atcccaaata ggtgggtcaa
     3181 gtaaagcacc ttctagatca ggatctagag attcgacccc ttcaagacct gcccagcaac
     3241 cattaagtag acctatacag tctcctggcc gaaactcaat ttcccctggt agaaatggaa
     3301 taagtcctcc taacaaatta tctcaacttc caaggacatc atcccctagt actgcttcaa
     3361 ctaagtcctc aggttctgga aaaatgtcat atacatctcc aggtagacag atgagccaac
     3421 agaaccttac caaacaaaca ggtttatcca agaatgccag tagtattcca agaagtgagt
     3481 ctgcctccaa aggactaaat cagatgaata atggtaatgg agccaataaa aaggtagaac
     3541 tttctagaat gtcttcaact aaatcaagtg gaagtgaatc tgatagatca gaaagacctg
     3601 tattagtacg ccagtcaact ttcatcaaag aagctccaag cccaacctta agaagaaaat
     3661 tggaggaatc tgcttcattt gaatctcttt ctccatcatc tagaccagct tctcccacta
     3721 ggtcccaggc acaaactcca gttttaagtc cttcccttcc tgatatgtct ctatccacac
     3781 attcgtctgt tcaggctggt ggatggcgaa aactcccacc taatctcagt cccactatag
     3841 agtataatga tggaagacca gcaaagcgcc atgatattgc acggtctcat tctgaaagtc
     3901 cttctagact tccaatcaat aggtcaggaa cctggaaacg tgagcacagc aaacattcat
     3961 catcccttcc tcgagtaagc acttggagaa gaactggaag ttcatcttca attctttctg
     4021 cttcatcaga atccagtgaa aaagcaaaaa gtgaggatga aaaacatgtg aactctattt
     4081 caggaaccaa acaaagtaaa gaaaaccaag tatccgcaaa aggaacatgg agaaaaataa
     4141 aagaaaatga attttctccc acaaatagta cttctcagac cgtttcctca ggtgctacaa
     4201 atggtgctga atcaaagact ctaatttatc aaatggcacc tgctgtttct aaaacagagg
     4261 atgtttgggt gagaattgag gactgtccca ttaacaatcc tagatctgga agatctccca
     4321 caggtaatac tcccccggtg attgacagtg tttcagaaaa ggcaaatcca aacattaaag
     4381 attcaaaaga taatcaggca aaacaaaatg tgggtaatgg cagtgttccc atgcgtaccg
     4441 tgggtttgga aaatcgcctg aactccttta ttcaggtgga tgcccctgac caaaaaggaa
     4501 ctgagataaa accaggacaa aataatcctg tccctgtatc agagactaat gaaagttcta
     4561 tagtggaacg taccccattc agttctagca gctcaagcaa acacagttca cctagtggga
     4621 ctgttgctgc cagagtgact ccttttaatt acaacccaag ccctaggaaa agcagcgcag
     4681 atagcacttc agctcggcca tctcagatcc caactccagt gaataacaac acaaagaagc
     4741 gagattccaa aactgacagc acagaatcca gtggaaccca aagtcctaag cgccattctg
     4801 ggtcttacct tgtgacatct gtttaaaaga gaggaagaat gaaactaaga aaattctatg
     4861 ttaattacaa ctgctatata gacattttgt ttcaaatgaa actttaaaag actgaaaaat
     4921 tttgtaaata ggtttgattc ttgttagagg gtttttgttc tcgaagccat atttgatagt
     4981 atactttgtc ttcactggtc ttattttggg aggcactctt gatggttagg aaaaaaatag
     5041 taaagccaag tatgtttgta cagtatgttt tacatgtatt taaagtagca tcccatccca
     5101 acttccttta attattgctt gtcttaaaat aatgaacact acagatagaa aatatgatat
     5161 attgctgtta tcaatcattt ctagattata aactgactaa acttacatca gggaaaaatt
     5221 ggtatttatg caaaaaaaaa tgtttttgtg gaattcctgc agcccggggg atccgtcgag
     5281 gaattcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc
     5341 ctggctcaca aataccactg agatcttttt ccctctgcca aaaattatgg ggacatcatg
     5401 aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg
     5461 tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa
     5521 catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg
     5581 aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc
     5641 ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta
     5701 tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc
     5761 ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc cctcgacgga
     5821 tcggccgcaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac
     5881 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     5941 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     6001 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     6061 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     6121 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     6181 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     6241 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     6301 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     6361 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     6421 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     6481 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     6541 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     6601 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     6661 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     6721 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     6781 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     6841 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     6901 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     6961 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     7021 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     7081 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     7141 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     7201 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     7261 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     7321 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     7381 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     7441 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     7501 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     7561 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     7621 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     7681 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     7741 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     7801 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     7861 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     7921 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     7981 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     8041 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     8101 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     8161 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     8221 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     8281 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
     8341 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     8401 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc
     8461 caagcttcac gctgccgcaa gcactcaggg cgcaagggct gctaaaggaa gcggaacacg
     8521 tagaaagcca gtccgcagaa acggtgctga ccccggatga atgtcagcta ctgggctatc
     8581 tggacaaggg aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg
     8641 cgatagctag actgggcggt tttatggaca gcaagcgaac cggaattgcc agctggggcg
     8701 ccctctggta aggttgggaa gccctgcaaa gtaaactgga tggctttctt gccgccaagg
     8761 atctgatggc gcaggggatc aagatcctgc ttcatccccg tggcccgttg ctcgcgtttg
     8821 ctggcggtgt ccccggaaga aatatatttg catgtcttta gttctatgat gacacaaacc
     8881 ccgcccagcg tcttgtcatt ggcgaattcg aacacgcaga tgcagtcggg gcggcgcggt
     8941 cccaggtcca cttcgcatat taaggtgacg cgtgtggcct cgaacaccga gcgaccctgc
     9001 agcgacccgc ttaacagcgt caacagcgtg ccgcagatct gatcaagaga caggatgagg
     9061 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     9121 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     9181 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     9241 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     9301 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     9361 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     9421 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     9481 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     9541 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     9601 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     9661 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     9721 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     9781 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     9841 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     9901 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     9961 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
    10021 gagttcttcg cccaccccgg gagatggggg aggctaactg aaacacggaa ggagacaata
    10081 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg
    10141 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg
    10201 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt
    10261 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caagccctgc catagccacg
    10321 ggccccgtgg gttagggacg gcggatcgcg gccctctaga gagcttggcc cattgcatac
    10381 gttgtatcca tatcataata tgtacattta tattggctca tgtccaacat taccgccatg
    10441 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
    10501 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
    10561 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
    10621 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
    10681 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
    10741 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
    10801 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
    10861 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
    10921 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
    10981 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
    11041 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg
    11101 atccagcctc cggtcgaccg atcctgagaa cttcagggtg agtttgggga cccttgattg
    11161 ttctttcttt ttcgctattg taaaattcat gttatatgga gggggcaaag ttttcagggt
    11221 gttgtttaga atgggaagat gtcccttgta tcaccatgga ccctcatgat aattttgttt
    11281 ctttcacttt ctactctgtt gacaaccatt gtctcctctt attttctttt cattttctgt
    11341 aactttttcg ttaaacttta gcttgcattt gtaacgaatt tttaaattca cttttgttta
    11401 tttgtcagat tgtaagtact ttctctaatc actttttttt caaggcaatc agggtatatt
    11461 atattgtact tcagcacagt tttagagaac aattgttata attaaatgat aaggtagaat
    11521 atttctgcat ataaattctg gctggcgtgg aaatattctt attggtagaa acaactacac
    11581 cctggtcatc atcctgcctt tctctttatg gttacaatga tatacactgt ttgagatgag
    11641 gataaaatac tctgagtcca aaccgggccc ctctgctaac catgttcatg ccttcttctc
    11701 tttcctacag ctcctgggca acgtgctggt tgttgtgctg tctcatcatt ttggcaaaga
    11761 attcctcgac ggatccgatc gaattcccgg aaatggggtc caagggtagc caaggatggc
    11821 tgcagcttca tatgatcagt tgttaaagca agttgaggca ctgaagatgg agaactcaaa
    11881 tcttcgacaa gagctagaag ataattccaa tcatcttaca aaactggaaa ctgaggcatc
    11941 taatatgaag gaagtactta aacaactaca aggaagtatt gaagatgaag ctatggcttc
    12001 ttctggacag attgatttat tagagcgtct taaagagctt aacttagata gcagtaattt
    12061 ccctggagta aaactgcggt caaaaatgtc cctccgttct tatggaagcc gggaaggatc
    12121 tgtatcaagc cgttctggag agtgcagtcc tgttcctatg ggttcatttc caagaagagg
    12181 gtttgtaaat ggaagcagag aaagtactgg atatttagaa gaacttgaga aagagaggtc
    12241 attgcttctt gctgatcttg acaaagaaga aaaggaaaaa gactggtatt acgctcaact
    12301 tcagaatctc actaaaagaa tagatagtct tcctttaact gaaaattttt ccttacaaac
    12361 agatatgacc agaaggcaat tggaatatga agcaaggcaa atcagagttg cgatggaaga
    12421 acaactaggt acctgccagg atatggaaaa acgagcacag cgaagaatag ccagaattca
    12481 gcaaatcgaa aaggacatac ttcgtatacg acagctttta cagtcccaag caacagaagc
    12541 agagaggtca tctcagaaca agcatgaaac cggctcacat gatgctgagc ggcagaatga
    12601 aggtcaagga gtgggagaaa tcaacatggc aacttctggt aatggtcagg gttcaactac
    12661 acgaatggac catgaaacag ccagtgtttt gagttctagt agcacacact ctgcacctcg
    12721 aaggctgaca agtcatctgg gaaccaaggt ggaaatggtg tattcattgt tgtcaatgct
    12781 tggtactcat gataaggatg atatgtcgcg aactttgcta gctatgtcta gctcccaaga
    12841 cagctgtata tccatgcgac agtctggatg tcttcctctc ctcatccagc ttttacatgg
    12901 caatgacaaa gactctgtat tgttgggaaa ttcccggggc agtaaagagg ctcgggccag
    12961 ggccagtgca gcactccaca acatcattca ctcacagcct gatgacaaga gaggcaggcg
    13021 tgaaatccga gtccttcatc ttttggaaca gatacgcgct tactgtgaaa cctgttggga
    13081 gtggcaggaa gctcatgaac caggcatgga ccaggacaaa aatccaatgc cagctcctgt
    13141 tgaacatcag atctgtcctg ctgtgtgtgt tctaatgaaa ctttcatttg atgaagagca
    13201 tagacatgca atgaatgaac tagggggact acaggccatt gcagaattat tgcaagtgga
    13261 ctgtgaaatg tatgggctta ctaatgacca ctacagtatt acactaagac gatatgctgg
    13321 aatggctttg acaaacttga cttttggaga tgtagccaac aaggctacgc tatgctctat
    13381 gaaaggctgc atgagagcac ttgtggccca actaaaatct gaaagtgaag acttacagca
    13441 ggttattgcg agtgttttga ggaatttgtc ttggcgagca gatgtaaata gtaaaaagac
    13501 gttgcgagaa gttggaagtg tgaaagcatt gatggaatgt gctttagaag ttaaaaagga
    13561 atcaaccctc aaaagcgtat tgagtgcctt atggaatttg tcagcacatt gcactgagaa
    13621 taaagctgat atatgtgctg tagatggtgc acttgcattt ttggttggca ctcttactta
    13681 ccggagccag acaaacactt tagccattat tgaaagtgga ggtgggatat tacggaatgt
    13741 gtccagcttg atagctacaa atgaggacca caggcaaatc ctaagagaga acaactgtct
    13801 acaaacttta ttacaacact taaaatctca tagtttgaca atagtcagta atgcatgtgg
    13861 aactttgtgg aatctctcag caagaaatcc taaagaccag gaagcattat gggacatggg
    13921 ggcagttagc atgctcaaga acctcattca ttcaaagcac aaaatgattg ctatgggaag
    13981 tgctgcagct ttaaggaatc tcatggcaaa taggcctgcg aagtacaagg atgccaatat
    14041 tatgtctcct ggctcaagct tgccatctct tcatgttagg aaacaaaaag ccctagaagc
    14101 agaattagat gctcagcact tatcagaaac ttttgacaat atagacaatt taagtcccaa
    14161 ggcatctcat cgtagtaagc agagacacaa gcaaagtctc tatggtgatt atgtttttga
    14221 caccaatcga catgatgata ataggtcaga caattttaat actggcaaca tgactgtcct
    14281 ttcaccatat ttgaatacta cagtgttacc cagctcctct tcatcaagag gaagcttaga
    14341 tagttctcgt tctgaaaaag atagaagttt ggagagagaa cgcggaattg gtctaggcaa
    14401 ctaccatcca gcaacagaaa atccaggaac ttcttcaaag cgaggtttgc agatctccac
    14461 cactgcagcc cagattgcca aagtcatgga agaagtgtca gccattcata cctctcagga
    14521 agacagaagt tctgggtcta ccactgaatt acattgtgtg acagatgaga gaaatgcact
    14581 tagaagaagc tctgctgccc atacacattc aaacacttac aatttcacta agtcggaaaa
    14641 ttcaaatagg acatgttcta tgccttatgc caaattagaa tacaagagat cttcaaatga
    14701 tagtttaaat agtgtcagta gtagtgatgg ttatggtaaa agaggtcaaa tgaaaccctc
    14761 gattgaatcc tattctgaag atgatgaaag taagttttgc agttatggtc aatacccagc
    14821 cgacctagcc cataaaatac atagtgcaaa tcatatggat gataatgatg gagaactaga
    14881 tacaccaata aattatagtc ttaaatattc agatgagcag ttgaactctg gaaggcaaag
    14941 tccttcacag aatgaaagat gggcaagacc caaacacata atagaagatg aaataaaaca
    15001 aagtgagcaa agacaatcaa ggaatcaaag tacaacttat cctgtttata ctgagagcac
    15061 tgatgataaa cacctcaagt tccaaccaca ttttggacag caggaatgtg tttctccata
    15121 caggtcacgg ggagccaatg gttcagaaac aaatcgagtg ggttctaatc atggaattaa
    15181 tcaaaatgta agccagtctt tgtgtcaaga agatgactat gaagatgata agcctaccaa
    15241 ttatagtgaa cgttactctg aagaagaaca gcatgaagaa gaagagagac caacaaatta
    15301 tagcataaaa tataatgaag agaaacgtca tgtggatcag cctattgatt atagtttaaa
    15361 atatgccaca gatattcctt catcacagaa acagtcattt tcattctcaa agagttcatc
    15421 tggacaaagc agtaaaaccg aacatatgtc ttcaagcagt gagaatacgt ccacaccttc
    15481 atctaatgcc aagaggcaga atcagctcca tccaagttct gc
//
