LOCUS       sequence-456525-        6099 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Cytokeratin, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6099)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 6099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Keratin14-C-4
FEATURES             Location/Qualifiers
     source          1..6099
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        43..234
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     191..211
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(236..340)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      367..822
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(454..473)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     664..685
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    829..950
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(840..859)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     894..913
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(2508..3218,3219..3221,3222..3224))
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2550..2571)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2899..2918
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3158..3179
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      3221..3230
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3269..3472)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3298..3318)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3473..3776
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(3951..4539)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4031..4050)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4868..4915
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4940..4959
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(5147..5941)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(5147..5941)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5258..5277)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5868..5887
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(join(5976..6099,1..234))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(5990..6099,1..26)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat ggttgctgac
       61 taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac
      121 ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg
      181 gactttccac accctaactg acacacattc cacagctggt tctttccgcc tcaggactct
      241 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
      301 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
      361 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
      421 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      481 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      541 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
      601 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
      661 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
      721 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
      781 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttacgcgtta agatacattg
      841 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
      901 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
      961 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
     1021 aaaacctcta caaatgtggt atggctgatt atgatcagtt atctagatcc ggtggatcct
     1081 cagttcttgg tgcgaaggac ctgctcgtgg gtggacacca ccttgccatc gtgcacatcc
     1141 atgaccttgg tgcggatttg gcggctggag gaggtcacat ctctggatga ctgcgatcca
     1201 gaggagaact gggaggagga gaggtgggcg tcctcgccct ccagcaggcg gcggtaggtg
     1261 gcgatctcct gctccagccg cgtcttcacg tccagcagga tcttgtactc ctggttctgc
     1321 tgctccatct cgcagcggag ctgggccagc tgctcctcca cgctgccaat catctcctgg
     1381 atctgggcca gctgcatgca gtagcgacct ttggtctcct ccaggctgtt ctccagggat
     1441 gctttcatgc tgagctggga ctgcagctca atctccaggt tctgcatggt gcgccggagc
     1501 tccgagatct cgctcttgcc gctctgcacc agctcgctgt tggtggccac ctcgcggttc
     1561 agctcctctg tcttggtgaa gaaccattcc tcggcatcct tgcggttctt ctctgccatc
     1621 ttctcatact ggtcacgcat ctcgttcaga atgcggctca ggtccacgcc aggtgcagcg
     1681 tccatctcca cattgacatc tccacccacc tggcctctca gggcattcat ctcctcctcg
     1741 tggttcttct tcaggtaggc cagctcctcc ttcaggctct caatctgcat ctccaggtca
     1801 gctctggcca gggtcagttc gtccagcacc ctgcgcaggc cattgatgtc ggcttccaca
     1861 ctcatgcgca ggttcaactc tgtctcatac ttggtgcgga agtcatccgc ggccagacgg
     1921 gcattgtcaa tctgcagaag gacattggca ttgtccactg tggctgtgag aatcttgttc
     1981 ctcaggtcct caatggtctt gaagtaggga ctgtagtctt tgatctcagc aggccgctgc
     2041 ctctggtacc agtcacggat cttcacttcc aggtcggcgt tggcctcctc cagagcacgc
     2101 accttgtcca ggtaggaggc caggcggtcg ttgaggttct gcatggtcac cttctcactg
     2161 cccaccagaa gcccatcacc accagcaaag ccaccaccaa agccaccacc caagccagca
     2221 ccaaggccac caccatatcc tcccccaaag ccactaccaa agctgctgct gctgctgctg
     2281 aagccaccgc catagccgcc ccccaaccca taggctcccc cagaggagaa gcgggaggat
     2341 gagacagaca ggccgccccc gtaggtgctg ggggcgcggc aggaccctcc ggccaggacg
     2401 gaggagatgc ggctggagcc gcccccgatg ccgcccccga tgccgcagga gcccttcatg
     2461 gagctggagg aggtgaactg gcggctgcag gtagtaccac ttccggactt gtacagctcg
     2521 tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc
     2581 ttctcgttgg ggtctttgct cagcttggac tgggtgctca ggtagtggtt gtcgggcagc
     2641 agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg
     2701 ccgtcctcga tgttgtggcg ggtcttgaag ttcaccttga tgccgttctt ctgcttgtcg
     2761 gcggtgatat agaccttgtg gctgttgtag ttgtactcca gcttgtgccc caggatgttg
     2821 ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg
     2881 aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc
     2941 tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg
     3001 tagcgggcga agcactgcac gccgtaggtc aaggtggtca cgagggtggg ccagggcacg
     3061 ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc
     3121 tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg
     3181 atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatggtggc gaccggtagc
     3241 gctagcggat ctgacggttc actaaaccag ctctgcttat atagacctcc caccgtacac
     3301 gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt
     3361 gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc
     3421 gtgagtcaaa ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa
     3481 tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact
     3541 gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca
     3601 tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac
     3661 gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg
     3721 ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga
     3781 actccatata tgggctatga actaatgacc ccgtaattga ttactattaa taactaatgc
     3841 atggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     3901 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     3961 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     4021 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     4081 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     4141 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     4201 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     4261 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     4321 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     4381 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     4441 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     4501 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     4561 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     4621 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     4681 agtatatatg agtaacctga ggctatggca gggcctgccg ccccgacgtt ggctgcgagc
     4741 cctgggcctt cacccgaact tggggggtgg ggtggggaaa aggaagaaac gcgggcgtat
     4801 tggccccaat ggggtctcgg tggggtatcg acagagtgcc agccctggga ccgaaccccg
     4861 cgtttatgaa caaacgaccc aacaccgtgc gttttattct gtctttttat tgccgtcata
     4921 gcgcgggttc cttccggtat tgtctccttc cgtgtttcag ttagcctccc cctagggtgg
     4981 gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc gtcccggaaa
     5041 acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc
     5101 aggttgggcg tcgcttggtc ggtcatttcg aaccccagag tcccgctcag aagaactcgt
     5161 caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga
     5221 ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta
     5281 tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc
     5341 cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc
     5401 cgtcgggcat gctcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct
     5461 cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga
     5521 tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc
     5581 gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat
     5641 cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga
     5701 gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt
     5761 gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg
     5821 ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc
     5881 cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca
     5941 tgcgaaacga tcctcatcct gtctcttgat cgatctttgc aaaagcctag gcctccaaaa
     6001 aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa
     6061 ataaaaaaaa ttagtcagcc atggggcgga gaatgggcg
//
