LOCUS       sequence-456529-        8454 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8454)
  AUTHORS   Sun Q, Westphal W, Wong KN, Tan I, Zhong Q
  TITLE     Rubicon controls endosome maturation as a Rab7 effector.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Nov 9. 107(45):19338-43.
  PUBMED    20974968
REFERENCE   2  (bases 1 to 8454)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Nov 9. 107(45):19338-43."
COMMENT     Sequence Label: EGFP-Rubicon deltaCT
FEATURES             Location/Qualifiers
     source          1..8454
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        234..437
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     388..408
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    439..457
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    460..478
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     488..512
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(603..605,606..608,609..1319)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(603..605,606..608,609..1319)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(648..669)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(909..928)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1256..1277
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3975..4040
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(4081..4098)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4087..4311
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4357..4785
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4444..4463)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4654..4675
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4794..4814)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4799..5128
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4979..5114
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5041..5060
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5176..5223
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5242..5616
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5746..5867
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5783..5802)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5837..5856
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5916..5932)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5916..5932)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5929..5951)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5940..5956
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5964..5970,5971..5988,5989..5994))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6009..6030
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6147..6164)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6318..6906)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6398..6417)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7077..7868,7869..7937))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7700..7719
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7938..8042)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8117..8136)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(8308..8454,1..233)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
       61 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
      121 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
      181 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      241 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      301 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      361 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
      421 tctatataag cagagctctc cctatcagtg atagagatct ccctatcagt gatagagatc
      481 gtcgacgagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
      541 acctccatag aagacaccgg gaccgatcca gcctccggac tctagcgttt aaacttaagc
      601 ttatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
      661 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
      721 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
      781 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
      841 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
      901 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
      961 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     1021 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     1081 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     1141 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     1201 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     1261 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg
     1321 gatccatgcg gccggagggc gcgggaatgg agctcggagg cggcgaggag cgcctgcctg
     1381 aggagagcag gagggagcac tggcagttgc tgggtaattt gaagacgacg gtggagggtt
     1441 tggtatcaac caacagcccc aacgtctggt ctaagtatgg tggcttggag cggctttgca
     1501 gggacatgca gagcatcctc tatcacgggc ttatccgtga ccaggcgtgc cgccgccaga
     1561 cggattactg gcagttcgtg aaagacatcc ggtggctcag tccccactca gcccttcacg
     1621 tggagaagtt catcagcgtg cacgagaacg accagagcag tgctgatggt gccagtgaac
     1681 gtgctgttgc cgagctgtgg ctgcagcaca gcctgcagta ccactgcctc tcagcccagc
     1741 tccggcccct gctcggggat agacagtata tcagaaaatt ctacacagat gctgccttcc
     1801 tgctaagtga cgctcatgtc acggccatgc tgcagtgcct ggaagccgtc gagcaaaata
     1861 atccccgcct cctggctcag atcgatgcgt ccatgtttgc cagaaagcac gagagcccgc
     1921 tcctggtgac aaagagccag agcctgacag ccctgcccag ttccacatac acccctccaa
     1981 acagctatgc tcagcattcc tactttgggt ccttctctag cctccaccaa tccgtgccca
     2041 acaatggctc agagagaaga tctacttcct ttccactctc tggccctccc cggaaacctc
     2101 aagaaagcag agggcacgtc tcaccagcag aggatcaaac catccaagcc cccccagttt
     2161 cagtctctgc actagccagg gattcccctt tgaccccaaa tgaaatgagc tccagtactc
     2221 tgaccagccc catagaggca tcctgggtca gcagccagaa tgattcccca ggtgatgcca
     2281 gtgaggggcc tgagtacctg gccattggca acttggaccc ccgaggccgg actgccagct
     2341 gtcagagtca cagcagcaat gccgagagca gcagttccaa tttgttctcc tccagcagct
     2401 cccagaagcc agattctgct gcctcttcct taggggacca ggaaggaggt ggggagagcc
     2461 agctgtccag tgtcctccgc aggtccagct tctcagaggg gcagacactc actgtcacca
     2521 gtggggcaaa gaaaagccac attcgctccc attcggatac cagcattgcc tccaggggag
     2581 ctccagaatc ctgcaatgat aaggcgaagt tgagaggccc tttgccctac tctggtcaaa
     2641 gcagtgaagt cagcacaccc agctctctgt acatggaata tgaaggtggt cggtacctgt
     2701 gctcagggga aggcatgttc cgaagaccat cagaaggaca gtccctcatc agctacctct
     2761 ctgagcaaga cttcggcagc tgtgccgacc tggaaaagga gaatgcccac ttcagcatct
     2821 cagagtcctt aattgctgcc atcgagctaa tgaagtgcaa catgatgagc cagtgcctag
     2881 aggaggagga agtggaagag gaagacagtg atagagagat ccaggagctg aagcagaaga
     2941 tccgccttcg gcgccagcaa atccgcacca agaacctgct ccccatgtac caggaggctg
     3001 agcacggaag ctttcgggtc acctccagca gctcccagtt cagctcacgt gattcggcac
     3061 agctctctga ctctggctct gctgatgagg ttgatgaatt tgaaatccaa gatgctgaca
     3121 tcagaaggaa cacagcctca agcagcaaat ccttcgtttc ctcccagtcc ttctcccact
     3181 gcttcctgca ctccacgtct gctgaggcgg tggccatggg gctcctgaag cagtttgagg
     3241 ggatgcagct tccagccgcc tcggagctgg agtggcttgt cccggagcat gatgcccctc
     3301 agaagctcct gcccattcct gactcactgc ccatctcacc ggatgacggg cagcacgctg
     3361 acatctacaa gctgcggatt cgtgttcgtg gcaacttgga gtgggccccg ccccggcctc
     3421 agataatttt taatgttcat ccagccccaa cgaggaaaat tgccgtggcc aagcagaatt
     3481 accgctgtgc aggatgtggc atccggactg accctgatta catcaagcga ctgcggtact
     3541 gtgagtacct gggcaagtac ttctgccagt gctgccacga gaatgcccag atggccatcc
     3601 ccagccgggt tctgcgcaag tgggacttca gcaagtacta cgtcagcaac ttctccaagg
     3661 acctgctcat taagatctgg aatgatcctc tcttcaacgt gcaggacata aacagtgccc
     3721 tctataggaa ggtcaagctg ctcaatcaag tccggctgct gcgggtccag ctgtgtcaca
     3781 tgaagaacat gttcaagact tgccgactgg ccaaggagct tctggattcc tttgacacag
     3841 tcccaggcca cctgacagag gacctccacc tgtactcact gaatgacctg actgcgacca
     3901 ggaaggggga gctggggccc cggcttgctg agctcaccag ggcaggggct acccatgtgg
     3961 agagaggcgg ccgcgactac aaagaccatg acggtgatta taaagatcat gacatcgact
     4021 acaaggatga cgatgacaag tgactcgagt ctagagggcc cgtttaaacc cgctgatcag
     4081 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     4141 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     4201 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     4261 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     4321 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     4381 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     4441 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     4501 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     4561 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     4621 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     4681 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     4741 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     4801 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     4861 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     4921 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     4981 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     5041 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     5101 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     5161 atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac
     5221 gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg
     5281 cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc
     5341 gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc
     5401 caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag
     5461 ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc
     5521 atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc
     5581 aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc
     5641 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     5701 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     5761 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     5821 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     5881 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     5941 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     6001 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     6061 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     6121 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     6181 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     6241 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     6301 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     6361 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     6421 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     6481 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     6541 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     6601 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     6661 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     6721 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     6781 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     6841 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     6901 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     6961 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     7021 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     7081 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     7141 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     7201 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     7261 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     7321 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     7381 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     7441 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     7501 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     7561 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     7621 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     7681 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     7741 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     7801 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     7861 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     7921 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     7981 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     8041 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc
     8101 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
     8161 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
     8221 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
     8281 gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat taatagtaat
     8341 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     8401 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataa
//
