LOCUS       sequence-456533-        8829 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8829)
  AUTHORS   Stempin CC, Chi L, Giraldo-Vela JP, High AA, Hacker H, Redecke V
  TITLE     The E3 Ubiquitin Ligase Mind Bomb-2 (MIB2) Protein Controls B-cell 
            CLL/Lymphoma 10 (BCL10)-dependent NF-{kappa}B Activation.
  JOURNAL   J Biol Chem. 2011 Oct 28;286(43):37147-57. Epub 2011 Sep 6.
  PUBMED    21896478
REFERENCE   2  (bases 1 to 8829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2011-10-28"; volume: "286(43)"; pages: "37147-57. 
            Epub 2011 Sep 6"
COMMENT     Sequence Label: p3HA-hMIB1
FEATURES             Location/Qualifiers
     source          1..8829
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(40..59)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        231..610
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        611..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     765..785
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     811..835
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        1045..1063
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1076..1095
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     5'UTR           1136..1178
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             1184..1210
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1214..1240
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1244..1270
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(4352..4368)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4352..4368)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(4378..4396)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4416..4433)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4422..4646
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4692..5120
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4779..4798)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4989..5010
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5129..5149)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5134..5463
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5314..5449
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5376..5395
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5530..6324
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5584..5603)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6194..6213
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6498..6619
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6535..6554)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6589..6608
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6668..6684)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6668..6684)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6681..6703)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6692..6708
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6716..6722,6723..6740,6741..6746))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6761..6782
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6899..6916)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7070..7658)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7150..7169)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7829..8620,8621..8689))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8452..8471
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8690..8794)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc
       61 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
      121 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
      181 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt
      241 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
      301 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      361 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      421 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      481 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      541 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      601 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      661 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      721 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      781 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg
      841 gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg
      901 cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg taagtaccgc
      961 ctatagagtc tataggccca cccccttggc ttcgttagaa cgcggctaca attaatacat
     1021 aaccttatgt atcatacaca tacgatttag gtgacactat agaataacat ccactttgcc
     1081 tttctctcca caggtgtcca ctcccaggtc caactgcacc tcggttctaa gcttgcttgt
     1141 tctttttgca gaagctcaga ataaacgctc aactttggcc atgtacccat acgatgttcc
     1201 agattacgcc atgtacccat acgatgttcc agattacgcc atgtacccat acgatgttcc
     1261 agattacgcc atgggtcata tggctagcgg atatcgaatg agtaactccc ggaataaccg
     1321 ggtgatggtg gaaggggttg gcgctcgggt agtgcgcggc ccggactgga agtgggggaa
     1381 gcaggacggc ggcgagggcc atgtgggcac cgtccggagc ttcgagagcc ccgaggaggt
     1441 ggtggtagtg tgggacaacg gcacagctgc caactaccgc tgctccgggg cttacgacct
     1501 ccgcatcctg gacagcgcgc ccaccggcat caagcatgat ggaaccatgt gtgatacctg
     1561 ccgccagcaa ccaatcattg gcattcgatg gaagtgtgca gagtgtacaa attatgattt
     1621 gtgcacagtg tgttatcatg gagataaaca tcatttaaga catcgctttt accgaattac
     1681 tacaccggga agtgagaggg ttctgttaga gtctcgtagg aaatctaaga agattacagc
     1741 cagaggaatc tttgcaggtg ccagagtggt gcgaggagtg gactggcagt gggaagatca
     1801 agatggagga aatggacgta ggggaaaggt aacagaaatc caggactgga gtgcatcaag
     1861 cccacatagc gcagcatatg tcctctggga taatggtgct aagaaccttt acagagttgg
     1921 ctttgagggc atgtctgatc tgaaatgtgt ccaggatgcc aagggaggtt ctttctacag
     1981 agatcactgc cctgtgctag gtgagcagaa tggcaacagg aatcctggtg gattgcagat
     2041 tggtgacctg gtaaatatag atctcgacct cgaaattgta cagtctttgc agcatggtca
     2101 tggaggatgg actgatggaa tgtttgagac tttaactaca actggaactg tttgtggcat
     2161 tgatgaagat catgacattg tagtacagta tccaagtggc aataggtgga ccttcaatcc
     2221 tgctgttctc actaaagcga acattgtccg aagtggagat gctgctcagg gtgcagaagg
     2281 aggcacctcg cagtttcaag tgggtgatct tgtacaagtt tgttatgacc tggaacgaat
     2341 taaacttcta caaagaggac atggagaatg ggctgaagcg atgcttccaa ctttaggtaa
     2401 agttggccga gtacaacaga tttattcaga cagtgattta aaggtggaag tttgtggaac
     2461 atcttggaca tacaatccag cagcagtttc caaggtggca tctgcaggat cagccattag
     2521 caatgcatct ggtgaaagac tctcacaact cctgaagaaa ttatttgaaa cccaagaatc
     2581 tggtgacctc aatgaagaat tagttaaggc tgctgccaat ggagatgttg ctaaagtgga
     2641 agatttgctt aaaagaccag atgtggatgt aaatgggcaa tgtgctggcc acacagctat
     2701 gcaagctgct agtcagaatg gacatgttga cattttgaag ttacttttga agcaaaacgt
     2761 ggatgtcgaa gcagaggata aagatggtga tagagcagtt caccatgcag cttttggaga
     2821 tgaaggcgct gttatagaag tactacatcg aggtagtgct gatttgaatg ctcgaaacaa
     2881 gcgccgacag acaccacttc atattgctgt caataaaggt catcttcaag ttgtgaagac
     2941 tttattggac tttggctgtc atcccagtct ccaggattct gaaggtgata cccctcttca
     3001 tgatgcaata agtaagaaac gtgatgatat cctagcagtt cttttggaag ctggagcaga
     3061 tgttaccatc acaaacaata atggatttaa tgctctgcat catgctgcac taaggggaaa
     3121 tcccagtgca atgcgtgttt tactatctaa attaccaaga ccatggattg tggatgagaa
     3181 gaaagatgat ggttatactg ccttacatct ggctgccctt aataatcacg tagaagtggc
     3241 tgaactgttg gtacatcagg gtaatgcaaa cctggatatc cagaatgtga accaacaaac
     3301 tgccctacac cttgctgttg aacgacagca tacccagatt gttaggcttt tggtccgtgc
     3361 aggtgccaag cttgatattc aggataagga tggggatact cctttgcatg aagctctaag
     3421 gcatcacact ttgtctcagc tacgtcagct ccaagatatg caagatgtgg ggaaggtgga
     3481 tgctgcctgg gagccatcca aaaacacgtt aataatggga cttggtaccc agggggcaga
     3541 gaagaagagt gcagcatcta ttgcctgttt cttggcagcc aatggtgctg acctgagcat
     3601 tcgaaataag aagggtcaat cgccacttga tctctgtcct gatccgaatc tctgcaaagc
     3661 actggcaaag tgtcataagg aaaaagtcag tggtcaagtg ggttctcgga gtccttctat
     3721 gattagtaat gattctgaaa ccttagaaga gtgtatggtg tgctcagata tgaagagaga
     3781 tactcttttt ggtccatgtg gacatattgc tacctgttct ttatgttctc cacgtgtcaa
     3841 gaaatgcctc atctgtaaag aacaggttca atccaggaca aagattgaag aatgtgtggt
     3901 atgctctgac aagaaagcag ctgttctttt tcaaccctgt ggccacatgt gtgcttgtga
     3961 gaactgtgct aacctgatga aaaagtgtgt gcagtgtcga gcagtagttg aacgaagagt
     4021 gcctttcatt atgtgctgtg gagggaaaag ttcagaagat gccactgatg atatctcaag
     4081 tgggaatatt ccagtattac aaaaggacaa ggataatacc aatgtcaatg cagatgtgca
     4141 aaagttgcag caacagttac aagacattaa agagcagaca atgtgccctg tgtgtctaga
     4201 tcgtctgaag aatatgattt tcctttgtgg tcacggaacc tgtcaactct gtggagaccg
     4261 catgagtgaa tgtcctatct gtcgcaaggc tattgaacga aggattcttt tgtattaaag
     4321 taaagaaagg gcgaattcct gcagcccggg ggatccacta gttctagagg gccctattct
     4381 atagtgtcac ctaaatgcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
     4441 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     4501 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     4561 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     4621 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     4681 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     4741 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     4801 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     4861 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     4921 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     4981 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     5041 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     5101 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     5161 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     5221 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     5281 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     5341 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     5401 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     5461 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga
     5521 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     5581 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     5641 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     5701 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     5761 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     5821 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     5881 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     5941 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     6001 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     6061 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     6121 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     6181 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     6241 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     6301 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
     6361 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     6421 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     6481 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     6541 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     6601 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     6661 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     6721 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     6781 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     6841 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     6901 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     6961 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     7021 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     7081 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     7141 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     7201 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     7261 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     7321 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     7381 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     7441 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     7501 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     7561 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     7621 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     7681 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     7741 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     7801 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     7861 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     7921 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     7981 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     8041 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     8101 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     8161 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     8221 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     8281 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     8341 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     8401 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     8461 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     8521 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     8581 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     8641 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     8701 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     8761 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     8821 acgtcgacg
//
