LOCUS       sequence-456537-        9083 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral stable expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9083)
  AUTHORS   Wang S, Tsun ZY, Wolfson RL, Shen K, Wyant GA, Plovanich ME, Yuan 
            ED, Jones TD, Chantranupong L, Comb W, Wang T, Bar-Peled L, Zoncu R,
            Straub C, Kim C, Park J, Sabatini BL, Sabatini DM
  TITLE     Metabolism. Lysosomal amino acid transporter SLC38A9 signals 
            arginine sufficiency to mTORC1.
  JOURNAL   Science. 2015 Jan 9;347(6218):188-94. doi: 10.1126/science.1257132. 
            Epub 2015 Jan 7.
  PUBMED    25567906
REFERENCE   2  (bases 1 to 9083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1257132"; journalName: "Science"; date: "2015-01-9-
            9"; volume: "347"; issue: "6218"; pages: "188-94"
COMMENT     Sequence Label: pLJM60-FLAG-SLC38A9.1
FEATURES             Location/Qualifiers
     source          1..9083
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    2..23
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(38..43,44..61,62..68)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    76..92
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     81..103
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     100..116
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     100..116
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     135..155
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        137..155
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        183..409
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             410..590
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    637..762
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1259..1492
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1677..1721
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2028..2231
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2182..2202
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2228..2252
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2462..2480
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2462..2479
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2493..2512
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             2571..2594
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    4359..4476
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4525..5035
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4954..4972
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5057..5656
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5057..5076)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5553..5573
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5784..6017
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6089..6210
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6126..6145)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6180..6199
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6250..6385
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6405..6424)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6406..6424)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6434..6451)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6434..6450)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6443..6465)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6592..7047
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6679..6698)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6889..6910
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7073..7177
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7178..7246,7247..8038)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7396..7415)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8209..8797
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8698..8717
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8951..8968
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
       61 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
      121 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
      181 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
      241 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      301 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      361 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      421 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      481 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      541 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      601 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      661 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      721 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      781 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      841 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      901 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      961 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     1021 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     1081 aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga
     1141 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     1201 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     1261 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     1321 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     1381 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     1441 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     1501 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     1561 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     1621 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     1681 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     1741 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     1801 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     1861 cgtttcagac ccacctccca accccgaggg gaccccatat gccaagtacg ccccctattg
     1921 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1981 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     2041 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     2101 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     2161 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     2221 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     2281 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
     2341 cgcggattcc ccgtgccaag agtgacgtaa gtaccgccta tagagtctat aggcccaccc
     2401 ccttggcttc gttagaacgc ggctacaatt aatacataac cttatgtatc atacacatac
     2461 gatttaggtg acactataga ataacatcca ctttgccttt ctctccacag gtgtccactc
     2521 ccaggtccaa ctgcacctcg gttctatcga ttgaattcac catggaaatg gactacaagg
     2581 atgacgatga caagggtggt ggtggtggtg cgtcgacaat ggctaacatg aacagtgact
     2641 caagacatct gggaacaagc gaggtggacc acgagcggga ccctggccct atgaacattc
     2701 agtttgagcc ctccgacctg cggtctaaga gacccttctg catcgaacct accaacattg
     2761 tgaacgtcaa tcacgtgatc cagagggtca gtgatcatgc atcagccatg aataagcgaa
     2821 ttcactacta ttcccggctg accacacccg ctgacaaagc actgatcgcc cctgatcatg
     2881 tggtcccagc acccgaggaa tgttacgtgt atagccctct gggatccgcc tacaagctgc
     2941 agagctacac cgaggggtat ggaaaaaaca catccctggt gactattttt atgatctgga
     3001 ataccatgat ggggacaagt attctgtcaa tcccatgggg aatcaagcag gccgggttca
     3061 ctaccggaat gtgcgtgatc attctgatgg gcctgctgac cctgtactgc tgttataggg
     3121 tggtcaaatc tcgcacaatg atgttttcac tggacacaac tagctgggag taccccgatg
     3181 tgtgtcggca ctattttgga agcttcggcc agtggagctc cctgctgttc agcctggtgt
     3241 ccctgattgg cgctatgatc gtgtactggg tcctgatgag caacttcctg tttaataccg
     3301 ggaagttcat ctttaacttc atccaccata tcaacgacac cgatacaatc ctgtctacaa
     3361 acaatagtaa ccctgtgatt tgcccatctg ccggaagtgg cgggcatcca gacaactcta
     3421 gtatgatctt ttacgctaat gatactggcg cacagcagtt cgagaagtgg tgggacaaaa
     3481 gcagaaccgt gcccttttat ctggtcggcc tgctgctgcc tctgctgaac tttaagtccc
     3541 catctttctt tagtaaattc aatatcctgg ggacagtgtc agtcctgtac ctgatttttc
     3601 tggtgacttt caaagcagtc cgcctgggat tccacctgga atttcattgg ttcatcccaa
     3661 ctgagttctt tgtgcccgaa attcgatttc agttcccaca gctgactgga gtgctgaccc
     3721 tggccttctt tatccacaat tgcatcatta cactgctgaa gaacaataag aaacaggaga
     3781 acaatgtgcg ggacctgtgt atcgcttata tgctggtcac tctgacctac ctgtatattg
     3841 gggtgctggt ctttgcatca ttccctagcc cccctctgtc taaagattgt atcgaacaga
     3901 actttctgga caatttccca tcaagcgata cactgagttt cattgccaga atctttctgc
     3961 tgttccagat gatgactgtg taccccctgc tggggtatct ggctagggtc cagctgctgg
     4021 gccacatttt tggggatatc taccctagca ttttccatgt gctgatcctg aacctgatca
     4081 ttgtgggcgc cggggtcatc atggcttgct tctaccctaa tattggaggc atcattaggt
     4141 attccggagc cgcttgtggc ctggcttttg tgttcatcta cccatccctg atctacatca
     4201 tttctctgca ccaggaggaa cgcctgacct ggcccaagct gatctttcat gtgttcatca
     4261 ttatcctggg cgtggcaaac ctgattgtcc agttctttat gtgagcggcc gcagtgagaa
     4321 ttctcgacct cgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     4381 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     4441 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     4501 gatccacttt ggccgcggct cgagggggtt ggggttgcgc cttttccaag gcagccctgg
     4561 gtttgcgcag ggacgcggct gctctgggcg tggttccggg aaacgcagcg gcgccgaccc
     4621 tgggtctcgc acattcttca cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct
     4681 tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt
     4741 cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc actagtaccc tcgcagacgg
     4801 acagcgccag ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca atagcggctg
     4861 ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg
     4921 ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc gcattctgca agcctccgga
     4981 gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc gacctctctc cccaggggga
     5041 tccaccggag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     5101 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     5161 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     5221 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     5281 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     5341 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     5401 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     5461 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     5521 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     5581 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     5641 caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg
     5701 accccatgca tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     5761 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     5821 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     5881 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     5941 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     6001 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     6061 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     6121 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     6181 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     6241 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     6301 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     6361 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt
     6421 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     6481 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     6541 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
     6601 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     6661 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     6721 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     6781 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     6841 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     6901 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     6961 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     7021 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
     7081 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     7141 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     7201 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     7261 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     7321 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     7381 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     7441 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     7501 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     7561 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     7621 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     7681 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     7741 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     7801 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     7861 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     7921 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     7981 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     8041 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     8101 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     8161 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     8221 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     8281 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     8341 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     8401 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     8461 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     8521 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     8581 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     8641 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     8701 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     8761 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     8821 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     8881 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     8941 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     9001 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     9061 agcgggcagt gagcgcaacg caa
//
