LOCUS       sequence-456549-        8701 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses PLZF in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8701)
  AUTHORS   Cassady JP, D'Alessio AC, Sarkar S, Dani VS, Fan ZP, Ganz K, 
            Roessler R, Sur M, Young RA, Jaenisch R
  TITLE     Direct Lineage Conversion of Adult Mouse Liver Cells and B 
            Lymphocytes to Neural Stem Cells.
  JOURNAL   Stem Cell Reports. 2014 Nov 6. pii: S2213-6711(14)00307-5. doi: 
            10.1016/j.stemcr.2014.10.001.
  PUBMED    25454632
REFERENCE   2  (bases 1 to 8701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2014 Nov 6. pii: S2213-6711(14)00307-5. doi: 
            10.1016/j.stemcr.2014.10.001."
COMMENT     Sequence Label: TetO-FUW-PLZF
FEATURES             Location/Qualifiers
     source          1..8701
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..182
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(200..398)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        400..779
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     951..970
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1045..1149
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1150..1218,1219..2010)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1368..1387)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2181..2769
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2670..2689
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2923..2940
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3057..3078
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3093..3098,3099..3116,3117..3123)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3131..3147
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3136..3158
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3155..3171
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3155..3171
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    3220..3341
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3231..3250)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3285..3304
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(3471..3845)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(3864..3911)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(3959..4288)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3973..4108
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4027..4046)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     4273..4293
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(4302..4730)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4412..4433)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4624..4643
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    4776..5000
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     4989..5006
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             5032..5212
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     5717..5733
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     5718..5734
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    5737..6325
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5843..5854
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6252..6272
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     regulatory      7960..7969
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8368..8377
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(8456..8480)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(8477..8515)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    8548..8566
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8590..8608
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8632..8650
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8674..8692
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 ctgctagaga ttttccacac tgactaaaag ggtctgaggg atctctagtt accagagtca
       61 cacaacagac gggcacacac tacttgaagc actcaaggca agctttattg aggcttaagc
      121 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      181 ccagtacagg caaaacgcgc tgcttatata gacctcccac cgtacacgcc taccgcccat
      241 ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca
      301 aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg
      361 ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa
      421 tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca
      481 ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg
      541 atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt
      601 ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt
      661 gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg
      721 gctatgaact aatgaccccg taattgatta ctattaataa ctagtcaata atcaatgtca
      781 acgcgtatat ctggcccgta catcgcgaag cagcgcaaaa cgcctaaccc taagcagatt
      841 cttcatgcaa ttgtcggtca agccttgcct tgttgtagct taaattttgc tcgcgcacta
      901 ctcagcgacc tccaacacac aagcagggag cagatactgg cttaactatg cggcatcaga
      961 gcagattgta ctgagagtgc accatagggg atcgggagat ctcccgatcc gtcgacgtca
     1021 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     1081 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     1141 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     1201 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     1261 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     1321 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     1381 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     1441 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     1501 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     1561 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     1621 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     1681 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     1741 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     1801 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     1861 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     1921 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     1981 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     2041 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     2101 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     2161 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     2221 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     2281 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     2341 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     2401 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     2461 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     2521 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     2581 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     2641 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     2701 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     2761 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     2821 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     2881 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     2941 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     3001 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     3061 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     3121 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     3181 gccaagctct agctagaggt cgacggtata cagacatgat aagatacatt gatgagtttg
     3241 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     3301 ttgctttatt tgtaaccatt ataagctgca ataaacaagt tggggtgggc gaagaactcc
     3361 agcatgagat ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag
     3421 cccaaccttt catagaaggc ggcggtggaa tcgaaatctc gtagcacgtg tcagtcctgc
     3481 tcctcggcca cgaagtgcac gcagttgccg gccgggtcgc gcagggcgaa ctcccgcccc
     3541 cacggctgct cgccgatctc ggtcatggcc ggcccggagg cgtcccggaa gttcgtggac
     3601 acgacctccg accactcggc gtacagctcg tccaggccgc gcacccacac ccaggccagg
     3661 gtgttgtccg gcaccacctg gtcctggacc gcgctgatga acagggtcac gtcgtcccgg
     3721 accacaccgg cgaagtcgtc ctccacgaag tcccgggaga acccgagccg gtcggtccag
     3781 aactcgaccg ctccggcgac gtcgcgcgcg gtgagcaccg gaacggcact ggtcaacttg
     3841 gccatggttt agttcctcac cttgtcgtat tatactatgc cgatatacta tgccgatgat
     3901 taattgtcaa cacgtgctga tcagatccga aaatggatat acaagctccc gggagctttt
     3961 tgcaaaagcc taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggcag
     4021 aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg
     4081 cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg
     4141 actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac
     4201 acctggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg
     4261 gggactttcc acaccctaac tgacacacat tccacagaat taattcgcgt taaatttttg
     4321 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     4381 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     4441 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     4501 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     4561 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     4621 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     4681 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ggggataccc
     4741 cctagagccc cagctggttc tttccgcctc agaagccata gagcccaccg catccccagc
     4801 atgcctgcta ttgtcttccc aatcctcccc cttgctgtcc tgccccaccc caccccccag
     4861 aatagaatga cacctactca gacaatgcga tgcaatttcc tcattttatt aggaaaggac
     4921 agtgggagtg gcaccttcca gggtcaagga aggcacgggg gaggggcaaa caacagatgg
     4981 ctggcaacta gaaggcacag tcgaggctga tcagcgggtt taaacgggcc ctgctagaga
     5041 ttttccacac tgactaaaag ggtctgaggg atctctagtt accagagtca cacaacagac
     5101 gggcacacac tacttgaagc actcaaggca agctttattg aggcttaagc agtgggttcc
     5161 ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac ccagtacagt
     5221 ccggatgcag ctctcgggcc atgtgatgaa atgctaggcg gctgtcaaac ctccactcta
     5281 atacttctct ctccgggtca tccatcccat gcaggctcac agggtgtaac aagcgggtgt
     5341 tctctccttc attggcttct tctaccttct cttgctcaac tggtactagc ttgtagcacc
     5401 atccaaaggt cagtggatat ctgatccctg gccctggtgt gtagttctgc caatcaggga
     5461 agtagccttg tgtgtggtag atccacagat caaggatatc ttgtcttcgt tgggagtgaa
     5521 ttagcccttc cagtcccccc ttttctttta aaaagtggct aagatctaca gctgccttgt
     5581 aagtcattgg tcttaaaggt acctgaggtg tgactggaaa acccacctcc tcctcctctt
     5641 gtgcttctag ccaggcacaa tcagcattgg tagctgctgt attgctactt gtgattgctc
     5701 catgtttttc taggtctcga ggtcgacggt atcgatgcgg ggaggcggcc caaagggaga
     5761 tccgactcgt ctgagggcga aggcgaagac gcggaagagg ccgcagagcc ggcagcaggc
     5821 cgcgggaagg aaggtccgct ggattgaggg ccgaagggac gtagcagaag gacgtcccgc
     5881 gcagaatcca ggtggcaaca caggcgagca gccaaggaaa ggacgatgat ttccccgaca
     5941 acaccacgga attgtcagtg cccaacagcc gagcccctgt ccagcagcgg gcaaggcagg
     6001 cggcgatgag ttccgccgtg gcaataggga gggggaaagc gaaagtcccg gaaaggagct
     6061 gacaggtggt ggcaatgccc caaccagtgg gggttgcgtc agcaaacaca gtgcacacca
     6121 cgccacgttg cctgacaacg ggccacaact cctcataaag agacagcaac caggatttat
     6181 acaaggagga gaaaatgaaa gccatacggg aagcaatagc atgatacaaa ggcattaaag
     6241 cagcgtatcc acatagcgta aaaggagcaa catagttaag aataccagtc aatctttcac
     6301 aaattttgta atccagaggt tgattatcga taagcttgat atcgaattct cacacatagc
     6361 acaggtagag gtacgtcttc tctatcctcc agtcgggcgg gatctcctcg ggcttgtggc
     6421 ccttcatgtg cttctgcatg gaggagaggc tggggcagta ctctgtgcag atggtgcact
     6481 ggtagggcga ggcgccgttg tgcgttctca ggtgcttgat catggccgag tagtcccggg
     6541 agcgctggtg gcagagctta cactcaaagg gcttctcacc tgtgtgcacc ctatagtgcg
     6601 tctccagctg atgcttgagg ctgaacttct tgtcacagcc attgcactcg tagggtttct
     6661 cacccgtgtg gatgcgtttg tggctcttga gtgtgctctc atcccggaag cagctgccac
     6721 agaactcaca ctcgtagggg tggtcgcctg tatgtgagcg caggtggcgt ttgagagccg
     6781 tgtggctggg gaaggtgcgg ttgcactcac tgcagatgta gctgcgcacg cccgcgtgga
     6841 cctccatgtg ctgctgcagt gcgctctgcg cctggaagcg cttcccacac agcagacaga
     6901 agacggccat gtcagtgcca gtatgggtct gcctgtgtgt ctccagggca tcctccttcg
     6961 aaaactgtgc accgcactga tcacagacaa aggctttggc acccgctgaa tgagccagta
     7021 agtgcattct cagccgcaaa ctatccagga accgcttccc gcagagctcg cacccgtacg
     7081 tcttcatccc actgtgcagc ttcctgtgct gctccacagc ctcgttatca ggaagctcga
     7141 ccccacacac gctgcactgc tctccgatgg tccggctctc tgacttcatg cccacagcca
     7201 gctcccccag cttgctgaac agctccctct gggtgaagcc ctggggcagc agccccccat
     7261 aggtgctgaa gtccatggag acagccaggg caggctgcac gtggaggcca gaggtcacgg
     7321 aagacggcat gctcaataca gcgtcagcct tgtggttggg caacacggag tagatgccca
     7381 gatgcttctc aggcgggggt gctgggtgct caggtcggcc agtgggggcc tggccagcct
     7441 cagctggggg tggcacctgc tcggcactct cctctcgccc atagtgtagc tccctagcac
     7501 tggtgatgac gctgcttcga gtcggggtcc caggcccctc tttgcctctt tcctcaacct
     7561 tgtcccccat ccctcctgag atgctggact cggctgcccc agggctgtcc tggctgggca
     7621 cctcatccac ctgcatcatc tccgtcttca cctcagccac cccagggtgc cgcccacccc
     7681 cagccagagt tggctcctcg ggccctgcag gtggctgaag agttccctgc aggagagact
     7741 gtcctatggt catcaaactg tccactgcag ccttggtggg actcatggct gaaagaccaa
     7801 atgaagtgga gactgaaggg ctctggtcca ccatgggccc agggaggctc tgtccagcca
     7861 cactggcata cccactctcc tcgctggaat gcttcgagat gaagatgttc ttgaggtacc
     7921 gagccttgcg gtcctcttct tcctcggccc cgccatcggc catggtggcc tccgtgtcat
     7981 tgtcgtctga ggcctggatg gtctccagca tcttcaggca ctgttcctcc aggtactcga
     8041 tctccaggat ctcggccgca tacagcaggt catccaggtc ctccgccttg gcttgcagcg
     8101 tggctgtata tgcatactcc agaatctgct ggaaggtctt tggcgagagg aagtccaaag
     8161 tatagtgttg actattgcgg tggaagagga tctcaaacat cttgctggtg caggccagca
     8221 ccgtccggtg ggcgtggaac tcctggctgt ccaccatgat gaccacatcg cacaaagtcc
     8281 cggccagccg catctggttg gccttgcaca gtagccccgt ggggtggcta gggttctgca
     8341 gctggatcat gcccattttt gtcagatcca tggtggcgaa ttcggggccg cggaggctgg
     8401 atcggtcccg gtgtcttcta tggaggtcaa aacagcgtgg atggcgtctc caggcgatct
     8461 gacggttcac taaacgagct ctgcttatat aggcctccca ccgtacacgc ctacctcgac
     8521 ccgggtaccg agctcgactt tcacttttct ctatcactga tagggagtgg taaactcgac
     8581 tttcactttt ctctatcact gatagggagt ggtaaactcg actttcactt ttctctatca
     8641 ctgataggga gtggtaaact cgactttcac ttttctctat cactgatagg gagtggtaaa
     8701 c
//
